ID   MAGB2_HUMAN             Reviewed;         319 AA.
AC   O15479; O75860;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 3.
DT   27-MAR-2024, entry version 186.
DE   RecName: Full=Melanoma-associated antigen B2;
DE   AltName: Full=Cancer/testis antigen 3.2;
DE            Short=CT3.2;
DE   AltName: Full=DSS-AHC critical interval MAGE superfamily 6;
DE            Short=DAM6;
DE   AltName: Full=MAGE XP-2 antigen;
DE   AltName: Full=MAGE-B2 antigen;
GN   Name=MAGEB2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   PubMed=8535061; DOI=10.1007/bf00352360;
RA   Dabovic B., Zanaria E., Bardoni B., Lisa A., Bordignon C., Russo V.,
RA   Matessi C., Traversari C., Camerino G.;
RT   "A family of rapidly evolving genes from the sex reversal critical region
RT   in Xp21.";
RL   Mamm. Genome 6:571-580(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LYS-61.
RX   PubMed=9441743; DOI=10.1006/geno.1997.5052;
RA   Lurquin C., De Smet C., Brasseur F., Muscatelli F., Martelange V.,
RA   De Plaen E., Brasseur R., Monaco A.P., Boon T.;
RT   "Two members of the human MAGEB gene family located in Xp21.3 are expressed
RT   in tumors of various histological origins.";
RL   Genomics 46:397-408(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT LYS-61.
RX   PubMed=9538511; DOI=10.1006/mgme.1997.2639;
RA   McCurdy D.K., Tai L.-Q., Nguyen J., Wang Z., Yang H.M., Udar N., Naiem F.,
RA   Concannon P., Gatti R.A.;
RT   "MAGE Xp-2: a member of the MAGE gene family isolated from an expression
RT   library using systemic lupus erythematosus sera.";
RL   Mol. Genet. Metab. 63:3-13(1998).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15772651; DOI=10.1038/nature03440;
RA   Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA   Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA   Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA   Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA   Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA   Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA   Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA   Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA   Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA   Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA   Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA   Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA   Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA   Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA   Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA   Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA   Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA   Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA   Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA   Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA   Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA   Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA   Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA   Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA   Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA   Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA   Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA   Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA   Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA   Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA   McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA   Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA   Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA   Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA   Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA   Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA   Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA   Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA   Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA   Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA   d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA   Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA   Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA   Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA   Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA   Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA   Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA   Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA   Rogers J., Bentley D.R.;
RT   "The DNA sequence of the human X chromosome.";
RL   Nature 434:325-337(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LYS-61.
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-105, VARIANT
RP   [LARGE SCALE ANALYSIS] LYS-61, AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [7]
RP   FUNCTION, AND INTERACTION WITH TRIM28.
RX   PubMed=20864041; DOI=10.1016/j.molcel.2010.08.029;
RA   Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.;
RT   "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.";
RL   Mol. Cell 39:963-974(2010).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-105, VARIANT
RP   [LARGE SCALE ANALYSIS] LYS-61, AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RC   TISSUE=Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc
CC       finger-containing E3 ubiquitin-protein ligases. Proposed to act through
CC       recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at
CC       the E3:substrate complex. {ECO:0000269|PubMed:20864041}.
CC   -!- SUBUNIT: Interacts with TRIM28. {ECO:0000269|PubMed:20864041}.
CC   -!- INTERACTION:
CC       O15479; Q99856: ARID3A; NbExp=3; IntAct=EBI-1057615, EBI-5458244;
CC       O15479; Q96S94-5: CCNL2; NbExp=3; IntAct=EBI-1057615, EBI-12024864;
CC       O15479; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-1057615, EBI-742054;
CC       O15479; Q9BQ95: ECSIT; NbExp=3; IntAct=EBI-1057615, EBI-712452;
CC       O15479; P35637: FUS; NbExp=3; IntAct=EBI-1057615, EBI-400434;
CC       O15479; Q96KN7: RPGRIP1; NbExp=3; IntAct=EBI-1057615, EBI-1050213;
CC       O15479; Q99757: TXN2; NbExp=3; IntAct=EBI-1057615, EBI-2932492;
CC       O15479; O96006: ZBED1; NbExp=3; IntAct=EBI-1057615, EBI-740037;
CC   -!- TISSUE SPECIFICITY: Expressed in testis and placenta, and in a
CC       significant fraction of tumors of various histologic types.
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DR   EMBL; U93163; AAC23617.1; -; Genomic_DNA.
DR   EMBL; AF015766; AAD01565.1; -; mRNA.
DR   EMBL; AC005185; AAD10635.1; -; Genomic_DNA.
DR   EMBL; BC026071; AAH26071.1; -; mRNA.
DR   CCDS; CCDS14219.1; -.
DR   RefSeq; NP_002355.2; NM_002364.4.
DR   RefSeq; XP_011543814.1; XM_011545512.1.
DR   AlphaFoldDB; O15479; -.
DR   SMR; O15479; -.
DR   BioGRID; 110287; 359.
DR   IntAct; O15479; 25.
DR   MINT; O15479; -.
DR   STRING; 9606.ENSP00000368273; -.
DR   GlyGen; O15479; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; O15479; -.
DR   PhosphoSitePlus; O15479; -.
DR   BioMuta; MAGEB2; -.
DR   EPD; O15479; -.
DR   jPOST; O15479; -.
DR   MassIVE; O15479; -.
DR   MaxQB; O15479; -.
DR   PaxDb; 9606-ENSP00000368273; -.
DR   PeptideAtlas; O15479; -.
DR   ProteomicsDB; 48684; -.
DR   Pumba; O15479; -.
DR   Antibodypedia; 24654; 223 antibodies from 27 providers.
DR   DNASU; 4113; -.
DR   Ensembl; ENST00000378988.5; ENSP00000368273.4; ENSG00000099399.6.
DR   GeneID; 4113; -.
DR   KEGG; hsa:4113; -.
DR   MANE-Select; ENST00000378988.5; ENSP00000368273.4; NM_002364.5; NP_002355.2.
DR   UCSC; uc004dbz.4; human.
DR   AGR; HGNC:6809; -.
DR   CTD; 4113; -.
DR   DisGeNET; 4113; -.
DR   GeneCards; MAGEB2; -.
DR   HGNC; HGNC:6809; MAGEB2.
DR   HPA; ENSG00000099399; Tissue enriched (testis).
DR   MIM; 300098; gene.
DR   neXtProt; NX_O15479; -.
DR   OpenTargets; ENSG00000099399; -.
DR   PharmGKB; PA30555; -.
DR   VEuPathDB; HostDB:ENSG00000099399; -.
DR   eggNOG; KOG4562; Eukaryota.
DR   GeneTree; ENSGT00940000155485; -.
DR   HOGENOM; CLU_039582_1_0_1; -.
DR   InParanoid; O15479; -.
DR   OMA; ATKMYIC; -.
DR   OrthoDB; 3128316at2759; -.
DR   PhylomeDB; O15479; -.
DR   TreeFam; TF328505; -.
DR   PathwayCommons; O15479; -.
DR   SignaLink; O15479; -.
DR   BioGRID-ORCS; 4113; 5 hits in 778 CRISPR screens.
DR   ChiTaRS; MAGEB2; human.
DR   GeneWiki; MAGEB2; -.
DR   GenomeRNAi; 4113; -.
DR   Pharos; O15479; Tbio.
DR   PRO; PR:O15479; -.
DR   Proteomes; UP000005640; Chromosome X.
DR   RNAct; O15479; Protein.
DR   Bgee; ENSG00000099399; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 34 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.1200; MAGE homology domain, winged helix WH1 motif; 1.
DR   Gene3D; 1.10.10.1210; MAGE homology domain, winged helix WH2 motif; 1.
DR   InterPro; IPR037445; MAGE.
DR   InterPro; IPR021072; MAGE_N.
DR   InterPro; IPR041898; MAGE_WH1.
DR   InterPro; IPR041899; MAGE_WH2.
DR   InterPro; IPR002190; MHD_dom.
DR   PANTHER; PTHR11736:SF152; MELANOMA-ASSOCIATED ANTIGEN B2; 1.
DR   PANTHER; PTHR11736; MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN; 1.
DR   Pfam; PF01454; MAGE; 1.
DR   Pfam; PF12440; MAGE_N; 1.
DR   SMART; SM01373; MAGE; 1.
DR   SMART; SM01392; MAGE_N; 1.
DR   PROSITE; PS50838; MAGE; 1.
DR   Genevisible; O15479; HS.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome; Tumor antigen; Ubl conjugation pathway.
FT   CHAIN           1..319
FT                   /note="Melanoma-associated antigen B2"
FT                   /id="PRO_0000156713"
FT   DOMAIN          111..310
FT                   /note="MAGE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT   REGION          1..112
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        44..58
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        90..112
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         77
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648,
FT                   ECO:0007744|PubMed:23186163"
FT   MOD_RES         105
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648,
FT                   ECO:0007744|PubMed:23186163"
FT   VARIANT         61
FT                   /note="E -> K (in dbSNP:rs2529541)"
FT                   /evidence="ECO:0000269|PubMed:15489334,
FT                   ECO:0000269|PubMed:9441743, ECO:0000269|PubMed:9538511,
FT                   ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163"
FT                   /id="VAR_027675"
FT   VARIANT         318
FT                   /note="G -> R (in dbSNP:rs5972090)"
FT                   /id="VAR_027676"
SQ   SEQUENCE   319 AA;  35277 MW;  CF0BD27A832D5CBB CRC64;
     MPRGQKSKLR AREKRRKARD ETRGLNVPQV TEAEEEEAPC CSSSVSGGAA SSSPAAGIPQ
     EPQRAPTTAA AAAAGVSSTK SKKGAKSHQG EKNASSSQAS TSTKSPSEDP LTRKSGSLVQ
     FLLYKYKIKK SVTKGEMLKI VGKRFREHFP EILKKASEGL SVVFGLELNK VNPNGHTYTF
     IDKVDLTDEE SLLSSWDFPR RKLLMPLLGV IFLNGNSATE EEIWEFLNML GVYDGEEHSV
     FGEPWKLITK DLVQEKYLEY KQVPSSDPPR FQFLWGPRAY AETSKMKVLE FLAKVNGTTP
     CAFPTHYEEA LKDEEKAGV
//