O15479 (MAGB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-associated antigen B2

Alternative name(s):
Cancer/testis antigen 3.2
Short name=CT3.2
DSS-AHC critical interval MAGE superfamily 6
Short name=DAM6
MAGE XP-2 antigen
MAGE-B2 antigen

Gene names

Name:

MAGEB2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Ref.7
Subunit structure	Interacts with TRIM28. Ref.7
Tissue specificity	Expressed in testis and placenta, and in a significant fraction of tumors of various histologic types.
Sequence similarities	Contains 1 MAGE domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Polymorphism
Molecular function	Tumor antigen
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Melanoma-associated antigen B2		PRO_0000156713
Regions
	Domain	111 – 310	200	MAGE
Amino acid modifications
	Modified residue	77	1	Phosphoserine Ref.6
	Modified residue	105	1	Phosphoserine Ref.6
Natural variations
	Natural variant	61	1	E → K. Ref.2 Ref.3 Ref.5 Ref.6 Corresponds to variant rs2529541 [ dbSNP | Ensembl ].		VAR_027675
	Natural variant	318	1	G → R. Corresponds to variant rs5972090 [ dbSNP | Ensembl ].		VAR_027676

Sequences

	Sequence		Length	Mass (Da)
	O15479 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: CF0BD27A832D5CBB	FASTA	319	35,277
	10 20 30 40 50 60 MPRGQKSKLR AREKRRKARD ETRGLNVPQV TEAEEEEAPC CSSSVSGGAA SSSPAAGIPQ 70 80 90 100 110 120 EPQRAPTTAA AAAAGVSSTK SKKGAKSHQG EKNASSSQAS TSTKSPSEDP LTRKSGSLVQ 130 140 150 160 170 180 FLLYKYKIKK SVTKGEMLKI VGKRFREHFP EILKKASEGL SVVFGLELNK VNPNGHTYTF 190 200 210 220 230 240 IDKVDLTDEE SLLSSWDFPR RKLLMPLLGV IFLNGNSATE EEIWEFLNML GVYDGEEHSV 250 260 270 280 290 300 FGEPWKLITK DLVQEKYLEY KQVPSSDPPR FQFLWGPRAY AETSKMKVLE FLAKVNGTTP 310 CAFPTHYEEA LKDEEKAGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of rapidly evolving genes from the sex reversal critical region in Xp21." Dabovic B., Zanaria E., Bardoni B., Lisa A., Bordignon C., Russo V., Matessi C., Traversari C., Camerino G. Mamm. Genome 6:571-580(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Two members of the human MAGEB gene family located in Xp21.3 are expressed in tumors of various histological origins." Lurquin C., De Smet C., Brasseur F., Muscatelli F., Martelange V., De Plaen E., Brasseur R., Monaco A.P., Boon T. Genomics 46:397-408(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-61.
[3]	"MAGE Xp-2: a member of the MAGE gene family isolated from an expression library using systemic lupus erythematosus sera." McCurdy D.K., Tai L.-Q., Nguyen J., Wang Z., Yang H.M., Udar N., Naiem F., Concannon P., Gatti R.A. Mol. Genet. Metab. 63:3-13(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-61.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-61. Tissue: Testis.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-105, VARIANT [LARGE SCALE ANALYSIS] LYS-61, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases." Doyle J.M., Gao J., Wang J., Yang M., Potts P.R. Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRIM28.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U93163 Genomic DNA. Translation: AAC23617.1. AF015766 mRNA. Translation: AAD01565.1. AC005185 Genomic DNA. Translation: AAD10635.1. BC026071 mRNA. Translation: AAH26071.1.
IPI	IPI00006726.
RefSeq	NP_002355.2. NM_002364.4.
UniGene	Hs.113824.
3D structure databases
ProteinModelPortal	O15479.
SMR	O15479. Positions 100-311.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15479. 2 interactions.
STRING	9606.ENSP00000368273.
PTM databases
PhosphoSite	O15479.
Proteomic databases
PaxDb	O15479.
PeptideAtlas	O15479.
PRIDE	O15479.
Protocols and materials databases
DNASU	4113.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378988; ENSP00000368273; ENSG00000099399.
GeneID	4113.
KEGG	hsa:4113.
UCSC	uc004dbz.3. human.
Organism-specific databases
CTD	4113.
GeneCards	GC0XP030233.
H-InvDB	HIX0016713.
HGNC	HGNC:6809. MAGEB2.
MIM	300098. gene.
neXtProt	NX_O15479.
PharmGKB	PA30555.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG320712.
HOGENOM	HOG000231161.
HOVERGEN	HBG006315.
InParanoid	O15479.
OMA	SSWDFPR.
OrthoDB	EOG42RD86.
PhylomeDB	O15479.
Gene expression databases
Bgee	O15479.
CleanEx	HS_MAGEB2.
Genevestigator	O15479.
GermOnline	ENSG00000099399. Homo sapiens.
Family and domain databases
InterPro	IPR002190. MAGE. IPR021072. Melanoma_ass_antigen_N. [Graphical view]
PANTHER	PTHR11736. PTHR11736. 1 hit.
Pfam	PF01454. MAGE. 1 hit. PF12440. MAGE_N. 1 hit. [Graphical view]
PROSITE	PS50838. MAGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4113.
NextBio	16150.
SOURCE	Search...

Entry information

Entry name

MAGB2_HUMAN

Accession

Primary (citable) accession number: O15479
Secondary accession number(s): O75860

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.