O15444 (CCL25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C motif chemokine 25

Alternative name(s):
Chemokine TECK
Small-inducible cytokine A25
Thymus-expressed chemokine

Gene names

Name:	CCL25
Synonyms:	SCYA25, TECK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
Subcellular location	Secreted.
Tissue specificity	Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 10544196. Source: ProtInc chemotaxis Traceable author statement PubMed 10640743. Source: ProtInc immune response Traceable author statement PubMed 10640743. Source: ProtInc inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW leukocyte migration Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	CCR10 chemokine receptor binding Inferred from direct assay PubMed 10706668. Source: BHF-UCL chemokine activity Traceable author statement PubMed 10640743. Source: ProtInc hormone activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O15444-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O15444-2)

Also known as: TECKvar;

The sequence of this isoform differs from the canonical sequence as follows:
     65-150: FYLPKRHRKV...SLLISANSGL → RPSCCKEVEFWKLQVIIIQV

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 3 (identifier: O15444-3)

The sequence of this isoform differs from the canonical sequence as follows:
     109-109: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 150	127	C-C motif chemokine 25		PRO_0000005235
Amino acid modifications
	Disulfide bond	30 ↔ 58		By similarity
	Disulfide bond	31 ↔ 75		By similarity
Natural variations
	Alternative sequence	65 – 150	86	FYLPK…ANSGL → RPSCCKEVEFWKLQVIIIQV in isoform 2.		VSP_001064
	Alternative sequence	109	1	Missing in isoform 3.		VSP_043199
	Natural variant	23	1	T → A. Corresponds to variant rs960173 [ dbSNP | Ensembl ].		VAR_044519
	Natural variant	101	1	H → R. Corresponds to variant rs2032887 [ dbSNP | Ensembl ].		VAR_044520
	Natural variant	104	1	T → M. Ref.1 Corresponds to variant rs1129763 [ dbSNP | Ensembl ].		VAR_044521

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 91D0810F137FBC7B	FASTA	150	16,609
	10 20 30 40 50 60 MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL 70 80 90 100 110 120 PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL HHNTQTFQAG PHAVKKLSSG 130 140 150 NSKLSSSKFS NPISSSKRNV SLLISANSGL
	Isoform 2 (TECKvar) [UniParc]. Checksum: 11307E0137B394D4	FASTA	84	9,496
	10 20 30 40 50 60 MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL 70 80 PAAIRPSCCK EVEFWKLQVI IIQV
	Isoform 3 [UniParc]. Checksum: AC5468E6EF71986E	FASTA	149	16,538
	10 20 30 40 50 60 MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL 70 80 90 100 110 120 PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL HHNTQTFQGP HAVKKLSSGN 130 140 SKLSSSKFSN PISSSKRNVS LLISANSGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development." Vicari A.P., Figueroa D.J., Hedrick J.A., Foster J.S., Singh K.P., Menon S., Copeland N.G., Gilbert D.J., Jenkins N.A., Bacon K.B., Zlotnik A. Immunity 7:291-301(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT MET-104. Tissue: Thymus.
[2]	"Molecular cloning and characterization of a splicing variant of chemokine TECK: a natural antagonist of human TECK." Hieshima K., Nakayama T., Fujisawa R., Izawa D., Yoshie O. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Thymus.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Lung.
[7]	"Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK." Zaballos A., Gutierrez J., Varona R., Ardavin C., Marquez G. J. Immunol. 162:5671-5675(1999) [PubMed] [Europe PMC] [Abstract] Cited for: RECEPTOR INTERACTION.
+	Additional computationally mapped references.

Web resources

Wikipedia

CCL25 entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86358 mRNA. Translation: AAB69981.1. AB046579 mRNA. Translation: BAB62257.1. AK292662 mRNA. Translation: BAF85351.1. AC008946 Genomic DNA. No translation available. CH471139 Genomic DNA. Translation: EAW68952.1. BC130561 mRNA. Translation: AAI30562.1. BC144463 mRNA. Translation: AAI44464.1.
IPI	IPI00292032. IPI00292033. IPI00792660.
RefSeq	NP_001188288.1. NM_001201359.1. NP_005615.2. NM_005624.3.
UniGene	Hs.310511.
3D structure databases
ProteinModelPortal	O15444.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5883N.
MINT	MINT-106224.
STRING	9606.ENSP00000375086.
PTM databases
PhosphoSite	O15444.
Proteomic databases
PaxDb	O15444.
PRIDE	O15444.
Protocols and materials databases
DNASU	6370.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253451; ENSP00000253451; ENSG00000131142. ENST00000390669; ENSP00000375086; ENSG00000131142.
GeneID	6370.
KEGG	hsa:6370.
UCSC	uc002mjc.4. human. uc002mjd.3. human.
Organism-specific databases
CTD	6370.
GeneCards	GC19P008117.
H-InvDB	HIX0039931.
HGNC	HGNC:10624. CCL25.
HPA	HPA055883.
MIM	602565. gene.
neXtProt	NX_O15444.
PharmGKB	PA35556.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39655.
HOGENOM	HOG000293281.
HOVERGEN	HBG050832.
KO	K13072.
OMA	QGVFEDC.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	O15444.
Bgee	O15444.
CleanEx	HS_CCL25.
Genevestigator	O15444.
Family and domain databases
InterPro	IPR001811. Chemokine_IL8-like_dom. [Graphical view]
Pfam	PF00048. IL8. 1 hit. [Graphical view]
SMART	SM00199. SCY. 1 hit. [Graphical view]
SUPFAM	SSF54117. Chemokine_IL8. 1 hit.
PROSITE	PS00472. SMALL_CYTOKINES_CC. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6370.
NextBio	24752.
SOURCE	Search...

Entry information

Entry name

CCL25_HUMAN

Accession

Primary (citable) accession number: O15444
Secondary accession number(s): A1L4J4 , A6NI52, A8K9E7, B5MCA5, Q96KJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 22, 2008
Last modified:	May 29, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.