O15440 (MRP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug resistance-associated protein 5

Alternative name(s):
ATP-binding cassette sub-family C member 5
Multi-specific organic anion transporter C
Short name=MOAT-C
SMRP
pABC11

Gene names

Name:	ABCC5
Synonyms:	MRP5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1437 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a multispecific organic anion pump which can transport nucleotide analogs. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc membrane fraction Inferred from direct assay. Source: MGI
Molecular function	ATP binding Traceable author statement. Source: ProtInc ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro organic anion transmembrane transporter activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1437	1437	Multidrug resistance-associated protein 5		PRO_0000093363
Regions
	Transmembrane	179 – 199	21	Helical; Potential
	Transmembrane	219 – 239	21	Helical; Potential
	Transmembrane	296 – 316	21	Helical; Potential
	Transmembrane	317 – 337	21	Helical; Potential
	Transmembrane	400 – 420	21	Helical; Potential
	Transmembrane	434 – 454	21	Helical; Potential
	Transmembrane	608 – 628	21	Helical; Potential
	Transmembrane	848 – 868	21	Helical; Potential
	Transmembrane	917 – 937	21	Helical; Potential
	Transmembrane	997 – 1017	21	Helical; Potential
	Transmembrane	1018 – 1038	21	Helical; Potential
	Transmembrane	1104 – 1124	21	Helical; Potential
	Transmembrane	1127 – 1147	21	Helical; Potential
	Domain	179 – 459	281	ABC transmembrane type-1 1
	Domain	562 – 783	222	ABC transporter 1
	Domain	859 – 1155	297	ABC transmembrane type-1 2
	Domain	1193 – 1427	235	ABC transporter 2
	Nucleotide binding	595 – 602	8	ATP 1 Potential
	Nucleotide binding	1227 – 1234	8	ATP 2 Potential
Amino acid modifications
	Modified residue	505	1	Phosphoserine Ref.9
	Modified residue	509	1	Phosphoserine Ref.9
	Modified residue	513	1	Phosphothreonine Ref.9
	Glycosylation	494	1	N-linked (GlcNAc...) Potential
	Glycosylation	636	1	N-linked (GlcNAc...) Potential
	Glycosylation	684	1	N-linked (GlcNAc...) Potential
	Glycosylation	890	1	N-linked (GlcNAc...) Potential
	Glycosylation	897	1	N-linked (GlcNAc...) Potential
	Glycosylation	1044	1	N-linked (GlcNAc...) Potential
	Glycosylation	1329	1	N-linked (GlcNAc...) Potential
	Glycosylation	1417	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	176	1	R → P in BAA76608. Ref.3
	Sequence conflict	400	1	S → G in AAD04169. Ref.1
	Sequence conflict	581	1	I → V in AAD37716. Ref.2
	Sequence conflict	1383	1	T → N in BAA76608. Ref.3
	Sequence conflict	1383	1	T → N in BAA22887. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	O15440 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 00558076B3BB4C00	FASTA	1,437	160,660
	10 20 30 40 50 60 MKDIDIGKEY IIPSPGYRSV RERTSTSGTH RDREDSKFRR TRPLECQDAL ETAARAEGLS 70 80 90 100 110 120 LDASMHSQLR ILDEEHPKGK YHHGLSALKP IRTTSKHQHP VDNAGLFSCM TFSWLSSLAR 130 140 150 160 170 180 VAHKKGELSM EDVWSLSKHE SSDVNCRRLE RLWQEELNEV GPDAASLRRV VWIFCRTRLI 190 200 210 220 230 240 LSIVCLMITQ LAGFSGPAFM VKHLLEYTQA TESNLQYSLL LVLGLLLTEI VRSWSLALTW 250 260 270 280 290 300 ALNYRTGVRL RGAILTMAFK KILKLKNIKE KSLGELINIC SNDGQRMFEA AAVGSLLAGG 310 320 330 340 350 360 PVVAILGMIY NVIILGPTGF LGSAVFILFY PAMMFASRLT AYFRRKCVAA TDERVQKMNE 370 380 390 400 410 420 VLTYIKFIKM YAWVKAFSQS VQKIREEERR ILEKAGYFQS ITVGVAPIVV VIASVVTFSV 430 440 450 460 470 480 HMTLGFDLTA AQAFTVVTVF NSMTFALKVT PFSVKSLSEA SVAVDRFKSL FLMEEVHMIK 490 500 510 520 530 540 NKPASPHIKI EMKNATLAWD SSHSSIQNSP KLTPKMKKDK RASRGKKEKV RQLQRTEHQA 550 560 570 580 590 600 VLAEQKGHLL LDSDERPSPE EEEGKHIHLG HLRLQRTLHS IDLEIQEGKL VGICGSVGSG 610 620 630 640 650 660 KTSLISAILG QMTLLEGSIA ISGTFAYVAQ QAWILNATLR DNILFGKEYD EERYNSVLNS 670 680 690 700 710 720 CCLRPDLAIL PSSDLTEIGE RGANLSGGQR QRISLARALY SDRSIYILDD PLSALDAHVG 730 740 750 760 770 780 NHIFNSAIRK HLKSKTVLFV THQLQYLVDC DEVIFMKEGC ITERGTHEEL MNLNGDYATI 790 800 810 820 830 840 FNNLLLGETP PVEINSKKET SGSQKKSQDK GPKTGSVKKE KAVKPEEGQL VQLEEKGQGS 850 860 870 880 890 900 VPWSVYGVYI QAAGGPLAFL VIMALFMLNV GSTAFSTWWL SYWIKQGSGN TTVTRGNETS 910 920 930 940 950 960 VSDSMKDNPH MQYYASIYAL SMAVMLILKA IRGVVFVKGT LRASSRLHDE LFRRILRSPM 970 980 990 1000 1010 1020 KFFDTTPTGR ILNRFSKDMD EVDVRLPFQA EMFIQNVILV FFCVGMIAGV FPWFLVAVGP 1030 1040 1050 1060 1070 1080 LVILFSVLHI VSRVLIRELK RLDNITQSPF LSHITSSIQG LATIHAYNKG QEFLHRYQEL 1090 1100 1110 1120 1130 1140 LDDNQAPFFL FTCAMRWLAV RLDLISIALI TTTGLMIVLM HGQIPPAYAG LAISYAVQLT 1150 1160 1170 1180 1190 1200 GLFQFTVRLA SETEARFTSV ERINHYIKTL SLEAPARIKN KAPSPDWPQE GEVTFENAEM 1210 1220 1230 1240 1250 1260 RYRENLPLVL KKVSFTIKPK EKIGIVGRTG SGKSSLGMAL FRLVELSGGC IKIDGVRISD 1270 1280 1290 1300 1310 1320 IGLADLRSKL SIIPQEPVLF SGTVRSNLDP FNQYTEDQIW DALERTHMKE CIAQLPLKLE 1330 1340 1350 1360 1370 1380 SEVMENGDNF SVGERQLLCI ARALLRHCKI LILDEATAAM DTETDLLIQE TIREAFADCT 1390 1400 1410 1420 1430 MLTIAHRLHT VLGSDRIMVL AQGQVVEFDT PSVLLSNDSS RFYAMFAAAE NKVAVKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of MOAT-C and MOAT-D, new members of the MRP/cMOAT subfamily of transporter proteins." Belinsky M.G., Bain L.J., Balsara B.B., Testa J.R., Kruh G.D. J. Natl. Cancer Inst. 90:1735-1741(1998) [PubMed: 9827529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"pABC11 (also known as MOAT-C and MRP5), a member of the ABC family of proteins, has anion transporter activity but does not confer multidrug resistance when overexpressed in human embryonic kidney 293 cells." McAleer M.A., Breen M.A., White N.L., Matthews N. J. Biol. Chem. 274:23541-23548(1999) [PubMed: 10438534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Detailed structural analysis on both human MRP5 and mouse mrp5 transcripts." Suzuki T., Sasaki H., Kuh H.J., Agui M., Tatsumi Y., Tanabe S., Terada M., Saijo N., Nishio K. Gene 242:167-173(2000) [PubMed: 10721709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Multidrug-resistance protein 5 is a multispecific organic anion transporter able to transport nucleotide analogs." Wijnholds J., Mol C.A.A.M., van Deemter L., de Haas M., Scheffer G.L., Baas F., Beijnen J.H., Scheper R.J., Hatse S., De Clercq E., Balzarini J., Borst P. Proc. Natl. Acad. Sci. U.S.A. 97:7476-7481(2000) [PubMed: 10840050] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Brain and Ovarian carcinoma.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"cDNA cloning of a short type of multidrug resistance protein homologue, SMRP, from a human lung cancer cell line." Suzuki T., Nishio K., Sasaki H., Kurokawa H., Saito-Ohara F., Ikeuch T., Tanabe S., Terada M., Saijo N. Biochem. Biophys. Res. Commun. 238:790-794(1997) [PubMed: 9325169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 492-1437. Tissue: Bone marrow.
[8]	"Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines." Kool M., de Haas M., Scheffer G.L., Scheper R.J., van Eijk M.J., Juijn J.A., Baas F., Borst P. Cancer Res. 57:3537-3547(1997) [PubMed: 9270026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1216-1437. Tissue: Brain.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505; SER-509 AND THR-513, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF104942 mRNA. Translation: AAD04169.1. AF146074 mRNA. Translation: AAD37716.1. AB019002 mRNA. Translation: BAA76608.1. U83661 mRNA. Translation: AAB71758.2. CH471052 Genomic DNA. Translation: EAW78308.1. AB005659 mRNA. Translation: BAA22887.1. BC140771 mRNA. Translation: AAI40772.1.
IPI	IPI00385383.
PIR	JC5667.
RefSeq	NP_005679.2. NM_005688.2.
UniGene	Hs.368563. Hs.728765.
3D structure databases
ProteinModelPortal	O15440.
SMR	O15440. Positions 575-784, 843-1431.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15440. 5 interactions.
STRING	O15440.
Protein family/group databases
TCDB	3.A.1.208.15. ATP-binding cassette (ABC) superfamily.
PTM databases
PhosphoSite	O15440.
Proteomic databases
PRIDE	O15440.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334444; ENSP00000333926; ENSG00000114770.
GeneID	10057.
KEGG	hsa:10057.
UCSC	uc003fmg.1. human.
Organism-specific databases
CTD	10057.
GeneCards	GC03M183637.
H-InvDB	HIX0003906.
HGNC	HGNC:56. ABCC5.
MIM	605251. gene.
neXtProt	NX_O15440.
PharmGKB	PA395.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04546.
HOGENOM	HBG758042.
HOVERGEN	HBG108314.
InParanoid	O15440.
OMA	QEGEVTF.
OrthoDB	EOG408N77.
PhylomeDB	O15440.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	O15440.
Bgee	O15440.
CleanEx	HS_ABCC5.
Genevestigator	O15440.
GermOnline	ENSG00000114770. Homo sapiens.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K05668.
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	37997.
SOURCE	Search...

Entry information

Entry name

MRP5_HUMAN

Accession

Primary (citable) accession number: O15440
Secondary accession number(s): B9EIQ2 , O14517, Q9UN85, Q9UNP5, Q9UQC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.