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O15440 (MRP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance-associated protein 5
Alternative name(s):
ATP-binding cassette sub-family C member 5
Multi-specific organic anion transporter C
Short name=MOAT-C
SMRP
pABC11
Gene names
Name:ABCC5
Synonyms:MRP5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1437 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a multispecific organic anion pump which can transport nucleotide analogs. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

All isoforms are equally expressed in retina. Ref.5

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O15440-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O15440-2)

Also known as: SV1;

The sequence of this isoform differs from the canonical sequence as follows:
     198-225: AFMVKHLLEYTQATESNLQYSLLLVLGL → PSFGDCSISAEVCGNRLHCTAILLSCFT
     226-1437: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: O15440-3)

Also known as: SV2;

The sequence of this isoform differs from the canonical sequence as follows:
     198-212: AFMVKHLLEYTQATE → LAWCCQDLDLGGVSL
     213-225: Missing.
     226-1437: Missing.
Note: Predominant isoform in retinal pigment epithelium, bladder, and stomach.
Isoform 4 (identifier: O15440-4)

Also known as: SV3;

The sequence of this isoform differs from the canonical sequence as follows:
     198-208: AFMVKHLLEYT → NFQDGCILRSE
     209-225: Missing.
     226-1437: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14371437Multidrug resistance-associated protein 5
PRO_0000093363

Regions

Transmembrane179 – 19921Helical; Potential
Transmembrane219 – 23921Helical; Potential
Transmembrane296 – 31621Helical; Potential
Transmembrane317 – 33721Helical; Potential
Transmembrane400 – 42021Helical; Potential
Transmembrane434 – 45421Helical; Potential
Transmembrane608 – 62821Helical; Potential
Transmembrane848 – 86821Helical; Potential
Transmembrane917 – 93721Helical; Potential
Transmembrane997 – 101721Helical; Potential
Transmembrane1018 – 103821Helical; Potential
Transmembrane1104 – 112421Helical; Potential
Transmembrane1127 – 114721Helical; Potential
Domain179 – 459281ABC transmembrane type-1 1
Domain562 – 783222ABC transporter 1
Domain859 – 1155297ABC transmembrane type-1 2
Domain1193 – 1427235ABC transporter 2
Nucleotide binding595 – 6028ATP 1 Potential
Nucleotide binding1227 – 12348ATP 2 Potential

Amino acid modifications

Modified residue5091Phosphoserine Ref.11 Ref.12
Modified residue5131Phosphothreonine Ref.11
Glycosylation4941N-linked (GlcNAc...) Potential
Glycosylation6361N-linked (GlcNAc...) Potential
Glycosylation6841N-linked (GlcNAc...) Potential
Glycosylation8901N-linked (GlcNAc...) Potential
Glycosylation8971N-linked (GlcNAc...) Potential
Glycosylation10441N-linked (GlcNAc...) Potential
Glycosylation13291N-linked (GlcNAc...) Potential
Glycosylation14171N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence198 – 22528AFMVK…LVLGL → PSFGDCSISAEVCGNRLHCT AILLSCFT in isoform 2.
VSP_043397
Alternative sequence198 – 21215AFMVK…TQATE → LAWCCQDLDLGGVSL in isoform 3.
VSP_043398
Alternative sequence198 – 20811AFMVKHLLEYT → NFQDGCILRSE in isoform 4.
VSP_043399
Alternative sequence209 – 22517Missing in isoform 4.
VSP_043400
Alternative sequence213 – 22513Missing in isoform 3.
VSP_043401
Alternative sequence226 – 14371212Missing in isoform 2, isoform 3 and isoform 4.
VSP_043402

Experimental info

Sequence conflict1761R → P in BAA76608. Ref.3
Sequence conflict4001S → G in AAD04169. Ref.1
Sequence conflict5811I → V in AAD37716. Ref.2
Sequence conflict13831T → N in BAA76608. Ref.3
Sequence conflict13831T → N in BAA22887. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 00558076B3BB4C00

FASTA1,437160,660
        10         20         30         40         50         60 
MKDIDIGKEY IIPSPGYRSV RERTSTSGTH RDREDSKFRR TRPLECQDAL ETAARAEGLS 

        70         80         90        100        110        120 
LDASMHSQLR ILDEEHPKGK YHHGLSALKP IRTTSKHQHP VDNAGLFSCM TFSWLSSLAR 

       130        140        150        160        170        180 
VAHKKGELSM EDVWSLSKHE SSDVNCRRLE RLWQEELNEV GPDAASLRRV VWIFCRTRLI 

       190        200        210        220        230        240 
LSIVCLMITQ LAGFSGPAFM VKHLLEYTQA TESNLQYSLL LVLGLLLTEI VRSWSLALTW 

       250        260        270        280        290        300 
ALNYRTGVRL RGAILTMAFK KILKLKNIKE KSLGELINIC SNDGQRMFEA AAVGSLLAGG 

       310        320        330        340        350        360 
PVVAILGMIY NVIILGPTGF LGSAVFILFY PAMMFASRLT AYFRRKCVAA TDERVQKMNE 

       370        380        390        400        410        420 
VLTYIKFIKM YAWVKAFSQS VQKIREEERR ILEKAGYFQS ITVGVAPIVV VIASVVTFSV 

       430        440        450        460        470        480 
HMTLGFDLTA AQAFTVVTVF NSMTFALKVT PFSVKSLSEA SVAVDRFKSL FLMEEVHMIK 

       490        500        510        520        530        540 
NKPASPHIKI EMKNATLAWD SSHSSIQNSP KLTPKMKKDK RASRGKKEKV RQLQRTEHQA 

       550        560        570        580        590        600 
VLAEQKGHLL LDSDERPSPE EEEGKHIHLG HLRLQRTLHS IDLEIQEGKL VGICGSVGSG 

       610        620        630        640        650        660 
KTSLISAILG QMTLLEGSIA ISGTFAYVAQ QAWILNATLR DNILFGKEYD EERYNSVLNS 

       670        680        690        700        710        720 
CCLRPDLAIL PSSDLTEIGE RGANLSGGQR QRISLARALY SDRSIYILDD PLSALDAHVG 

       730        740        750        760        770        780 
NHIFNSAIRK HLKSKTVLFV THQLQYLVDC DEVIFMKEGC ITERGTHEEL MNLNGDYATI 

       790        800        810        820        830        840 
FNNLLLGETP PVEINSKKET SGSQKKSQDK GPKTGSVKKE KAVKPEEGQL VQLEEKGQGS 

       850        860        870        880        890        900 
VPWSVYGVYI QAAGGPLAFL VIMALFMLNV GSTAFSTWWL SYWIKQGSGN TTVTRGNETS 

       910        920        930        940        950        960 
VSDSMKDNPH MQYYASIYAL SMAVMLILKA IRGVVFVKGT LRASSRLHDE LFRRILRSPM 

       970        980        990       1000       1010       1020 
KFFDTTPTGR ILNRFSKDMD EVDVRLPFQA EMFIQNVILV FFCVGMIAGV FPWFLVAVGP 

      1030       1040       1050       1060       1070       1080 
LVILFSVLHI VSRVLIRELK RLDNITQSPF LSHITSSIQG LATIHAYNKG QEFLHRYQEL 

      1090       1100       1110       1120       1130       1140 
LDDNQAPFFL FTCAMRWLAV RLDLISIALI TTTGLMIVLM HGQIPPAYAG LAISYAVQLT 

      1150       1160       1170       1180       1190       1200 
GLFQFTVRLA SETEARFTSV ERINHYIKTL SLEAPARIKN KAPSPDWPQE GEVTFENAEM 

      1210       1220       1230       1240       1250       1260 
RYRENLPLVL KKVSFTIKPK EKIGIVGRTG SGKSSLGMAL FRLVELSGGC IKIDGVRISD 

      1270       1280       1290       1300       1310       1320 
IGLADLRSKL SIIPQEPVLF SGTVRSNLDP FNQYTEDQIW DALERTHMKE CIAQLPLKLE 

      1330       1340       1350       1360       1370       1380 
SEVMENGDNF SVGERQLLCI ARALLRHCKI LILDEATAAM DTETDLLIQE TIREAFADCT 

      1390       1400       1410       1420       1430 
MLTIAHRLHT VLGSDRIMVL AQGQVVEFDT PSVLLSNDSS RFYAMFAAAE NKVAVKG 

« Hide

Isoform 2 (SV1) [UniParc].

Checksum: 771FBD2C32E08B8C
Show »

FASTA22525,341
Isoform 3 (SV2) [UniParc].

Checksum: D562C6FBF01922D1
Show »

FASTA21223,988
Isoform 4 (SV3) [UniParc].

Checksum: 3FF375FBCE128270
Show »

FASTA20823,677

References

« Hide 'large scale' references
[1]"Characterization of MOAT-C and MOAT-D, new members of the MRP/cMOAT subfamily of transporter proteins."
Belinsky M.G., Bain L.J., Balsara B.B., Testa J.R., Kruh G.D.
J. Natl. Cancer Inst. 90:1735-1741(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"pABC11 (also known as MOAT-C and MRP5), a member of the ABC family of proteins, has anion transporter activity but does not confer multidrug resistance when overexpressed in human embryonic kidney 293 cells."
McAleer M.A., Breen M.A., White N.L., Matthews N.
J. Biol. Chem. 274:23541-23548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Detailed structural analysis on both human MRP5 and mouse mrp5 transcripts."
Suzuki T., Sasaki H., Kuh H.J., Agui M., Tatsumi Y., Tanabe S., Terada M., Saijo N., Nishio K.
Gene 242:167-173(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Multidrug-resistance protein 5 is a multispecific organic anion transporter able to transport nucleotide analogs."
Wijnholds J., Mol C.A.A.M., van Deemter L., de Haas M., Scheffer G.L., Baas F., Beijnen J.H., Scheper R.J., Hatse S., De Clercq E., Balzarini J., Borst P.
Proc. Natl. Acad. Sci. U.S.A. 97:7476-7481(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
Tissue: Brain and Ovarian carcinoma.
[5]"Three novel ABCC5 splice variants in human retina and their role as regulators of ABCC5 gene expression."
Stojic J., Stohr H., Weber B.H.
BMC Mol. Biol. 8:42-42(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
Tissue: Retina.
[6]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Pancreas.
[9]"cDNA cloning of a short type of multidrug resistance protein homologue, SMRP, from a human lung cancer cell line."
Suzuki T., Nishio K., Sasaki H., Kurokawa H., Saito-Ohara F., Ikeuch T., Tanabe S., Terada M., Saijo N.
Biochem. Biophys. Res. Commun. 238:790-794(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 492-1437 (ISOFORM 1).
Tissue: Bone marrow.
[10]"Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines."
Kool M., de Haas M., Scheffer G.L., Scheper R.J., van Eijk M.J., Juijn J.A., Baas F., Borst P.
Cancer Res. 57:3537-3547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1216-1437 (ISOFORM 1).
Tissue: Brain.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND THR-513, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Web resources

ABCMdb

Database for mutations in ABC proteins

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF104942 mRNA. Translation: AAD04169.1.
AF146074 mRNA. Translation: AAD37716.1.
AB019002 mRNA. Translation: BAA76608.1.
U83661 mRNA. Translation: AAB71758.2.
AY754874 mRNA. Translation: AAW82948.1.
AY754875 mRNA. Translation: AAW82949.1.
AY754876 mRNA. Translation: AAW82950.1.
AC068644 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78308.1.
BC050744 mRNA. Translation: AAH50744.1.
BC140771 mRNA. Translation: AAI40772.1.
BC142719 mRNA. Translation: AAI42720.1.
AB005659 mRNA. Translation: BAA22887.1.
PIRJC5667.
RefSeqNP_001018881.1. NM_001023587.1.
NP_005679.2. NM_005688.2.
XP_005247115.1. XM_005247058.2.
XP_005247116.1. XM_005247059.2.
UniGeneHs.368563.
Hs.743953.

3D structure databases

ProteinModelPortalO15440.
SMRO15440. Positions 532-1432.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115368. 5 interactions.
IntActO15440. 5 interactions.
STRING9606.ENSP00000333926.

Chemistry

ChEMBLCHEMBL2046258.

Protein family/group databases

TCDB3.A.1.208.15. the atp-binding cassette (abc) superfamily.

PTM databases

PhosphoSiteO15440.

Proteomic databases

PaxDbO15440.
PRIDEO15440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000334444; ENSP00000333926; ENSG00000114770. [O15440-1]
ENST00000382494; ENSP00000371934; ENSG00000114770. [O15440-4]
ENST00000392579; ENSP00000376358; ENSG00000114770. [O15440-2]
ENST00000443376; ENSP00000416840; ENSG00000114770. [O15440-3]
GeneID10057.
KEGGhsa:10057.
UCSCuc003fmg.3. human. [O15440-1]
uc003fmh.3. human. [O15440-4]
uc010hxn.3. human. [O15440-2]

Organism-specific databases

CTD10057.
GeneCardsGC03M183637.
HGNCHGNC:56. ABCC5.
HPAHPA052295.
MIM605251. gene.
neXtProtNX_O15440.
PharmGKBPA395.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1132.
HOGENOMHOG000116132.
HOVERGENHBG108314.
InParanoidO15440.
KOK05668.
OMALFSVLHI.
OrthoDBEOG7MWGW0.
PhylomeDBO15440.
TreeFamTF105202.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressO15440.
BgeeO15440.
CleanExHS_ABCC5.
GenevestigatorO15440.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSABCC5. human.
GeneWikiABCC5.
GenomeRNAi10057.
NextBio37997.
PROO15440.
SOURCESearch...

Entry information

Entry nameMRP5_HUMAN
AccessionPrimary (citable) accession number: O15440
Secondary accession number(s): B9EIQ2 expand/collapse secondary AC list , O14517, Q29ZA9, Q29ZB1, Q86W30, Q9UN85, Q9UNP5, Q9UQC3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM