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O15427

- MOT4_HUMAN

UniProt

O15427 - MOT4_HUMAN

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Protein

Monocarboxylate transporter 4

Gene

SLC16A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).By similarity

GO - Molecular functioni

  1. monocarboxylic acid transmembrane transporter activity Source: ProtInc
  2. poly(A) RNA binding Source: UniProtKB
  3. secondary active monocarboxylate transmembrane transporter activity Source: InterPro
  4. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. cellular metabolic process Source: Reactome
  3. leukocyte migration Source: Reactome
  4. monocarboxylic acid transport Source: ProtInc
  5. pyruvate metabolic process Source: Reactome
  6. small molecule metabolic process Source: Reactome
  7. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_12560. Basigin interactions.
REACT_20515. Proton-coupled monocarboxylate transport.
REACT_2071. Pyruvate metabolism.

Protein family/group databases

TCDBi2.A.1.13.6. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 4
Short name:
MCT 4
Alternative name(s):
Solute carrier family 16 member 3
Gene namesi
Name:SLC16A3
Synonyms:MCT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:10924. SLC16A3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1716CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence AnalysisAdd
BLAST
Topological domaini39 – 6123ExtracellularSequence AnalysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Topological domaini83 – 842CytoplasmicSequence Analysis
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST
Topological domaini106 – 1094ExtracellularSequence Analysis
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Topological domaini131 – 14919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Topological domaini171 – 1799ExtracellularSequence Analysis
Transmembranei180 – 20021HelicalSequence AnalysisAdd
BLAST
Topological domaini201 – 22727CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei228 – 24821HelicalSequence AnalysisAdd
BLAST
Topological domaini249 – 26416ExtracellularSequence AnalysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence AnalysisAdd
BLAST
Topological domaini286 – 2949CytoplasmicSequence Analysis
Transmembranei295 – 31521HelicalSequence AnalysisAdd
BLAST
Topological domaini316 – 3172ExtracellularSequence Analysis
Transmembranei318 – 33821HelicalSequence AnalysisAdd
BLAST
Topological domaini339 – 35113CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei352 – 37221HelicalSequence AnalysisAdd
BLAST
Topological domaini373 – 38412ExtracellularSequence AnalysisAdd
BLAST
Transmembranei385 – 40521HelicalSequence AnalysisAdd
BLAST
Topological domaini406 – 46560CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. actin cytoskeleton Source: HPA
  2. integral component of plasma membrane Source: ProtInc
  3. membrane Source: ProtInc
  4. nuclear membrane Source: HPA
  5. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35815.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 465464Monocarboxylate transporter 4PRO_0000211394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei460 – 4601Phosphothreonine4 Publications
Modified residuei464 – 4641Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO15427.
PaxDbiO15427.
PeptideAtlasiO15427.
PRIDEiO15427.

PTM databases

PhosphoSiteiO15427.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle.

Gene expression databases

BgeeiO15427.
CleanExiHS_SLC16A3.
ExpressionAtlasiO15427. baseline and differential.
GenevestigatoriO15427.

Organism-specific databases

HPAiCAB017490.
HPA021451.

Interactioni

Protein-protein interaction databases

BioGridi114571. 1 interaction.
IntActiO15427. 1 interaction.
MINTiMINT-4526381.
STRINGi9606.ENSP00000376150.

Structurei

3D structure databases

ProteinModelPortaliO15427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG321880.
GeneTreeiENSGT00760000118926.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiO15427.
KOiK08180.
OMAiGNFFCIR.
PhylomeDBiO15427.
TreeFamiTF313792.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004743. Monocarb_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O15427-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGGAVVDEGP TGVKAPDGGW GWAVLFGCFV ITGFSYAFPK AVSVFFKELI
60 70 80 90 100
QEFGIGYSDT AWISSILLAM LYGTGPLCSV CVNRFGCRPV MLVGGLFASL
110 120 130 140 150
GMVAASFCRS IIQVYLTTGV ITGLGLALNF QPSLIMLNRY FSKRRPMANG
160 170 180 190 200
LAAAGSPVFL CALSPLGQLL QDRYGWRGGF LILGGLLLNC CVCAALMRPL
210 220 230 240 250
VVTAQPGSGP PRPSRRLLDL SVFRDRGFVL YAVAASVMVL GLFVPPVFVV
260 270 280 290 300
SYAKDLGVPD TKAAFLLTIL GFIDIFARPA AGFVAGLGKV RPYSVYLFSF
310 320 330 340 350
SMFFNGLADL AGSTAGDYGG LVVFCIFFGI SYGMVGALQF EVLMAIVGTH
360 370 380 390 400
KFSSAIGLVL LMEAVAVLVG PPSGGKLLDA THVYMYVFIL AGAEVLTSSL
410 420 430 440 450
ILLLGNFFCI RKKPKEPQPE VAAAEEEKLH KPPADSGVDL REVEHFLKAE
460
PEKNGEVVHT PETSV
Length:465
Mass (Da):49,469
Last modified:January 1, 1998 - v1
Checksum:i7BF6384B0F14D927
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81800 mRNA. Translation: AAC52015.1.
AK127319 mRNA. Translation: BAG54480.1.
BC112267 mRNA. Translation: AAI12268.1.
BC112269 mRNA. Translation: AAI12270.1.
CCDSiCCDS11804.1.
RefSeqiNP_001035887.1. NM_001042422.2.
NP_001035888.1. NM_001042423.2.
NP_001193879.1. NM_001206950.1.
NP_001193880.1. NM_001206951.1.
NP_001193881.1. NM_001206952.1.
NP_004198.1. NM_004207.3.
UniGeneiHs.500761.

Genome annotation databases

EnsembliENST00000392339; ENSP00000376150; ENSG00000141526.
ENST00000392341; ENSP00000376152; ENSG00000141526.
ENST00000581287; ENSP00000463978; ENSG00000141526.
ENST00000582743; ENSP00000462405; ENSG00000141526.
ENST00000617373; ENSP00000483212; ENSG00000141526.
ENST00000619321; ENSP00000482013; ENSG00000141526.
GeneIDi9123.
KEGGihsa:9123.
UCSCiuc002kea.3. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81800 mRNA. Translation: AAC52015.1 .
AK127319 mRNA. Translation: BAG54480.1 .
BC112267 mRNA. Translation: AAI12268.1 .
BC112269 mRNA. Translation: AAI12270.1 .
CCDSi CCDS11804.1.
RefSeqi NP_001035887.1. NM_001042422.2.
NP_001035888.1. NM_001042423.2.
NP_001193879.1. NM_001206950.1.
NP_001193880.1. NM_001206951.1.
NP_001193881.1. NM_001206952.1.
NP_004198.1. NM_004207.3.
UniGenei Hs.500761.

3D structure databases

ProteinModelPortali O15427.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114571. 1 interaction.
IntActi O15427. 1 interaction.
MINTi MINT-4526381.
STRINGi 9606.ENSP00000376150.

Chemistry

BindingDBi O15427.
ChEMBLi CHEMBL2073663.
DrugBanki DB01440. Gamma Hydroxybutyric Acid.
DB00627. Niacin.
DB00119. Pyruvic acid.

Protein family/group databases

TCDBi 2.A.1.13.6. the major facilitator superfamily (mfs).

PTM databases

PhosphoSitei O15427.

Proteomic databases

MaxQBi O15427.
PaxDbi O15427.
PeptideAtlasi O15427.
PRIDEi O15427.

Protocols and materials databases

DNASUi 9123.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000392339 ; ENSP00000376150 ; ENSG00000141526 .
ENST00000392341 ; ENSP00000376152 ; ENSG00000141526 .
ENST00000581287 ; ENSP00000463978 ; ENSG00000141526 .
ENST00000582743 ; ENSP00000462405 ; ENSG00000141526 .
ENST00000617373 ; ENSP00000483212 ; ENSG00000141526 .
ENST00000619321 ; ENSP00000482013 ; ENSG00000141526 .
GeneIDi 9123.
KEGGi hsa:9123.
UCSCi uc002kea.3. human.

Organism-specific databases

CTDi 9123.
GeneCardsi GC17P080186.
HGNCi HGNC:10924. SLC16A3.
HPAi CAB017490.
HPA021451.
MIMi 603877. gene.
neXtProti NX_O15427.
PharmGKBi PA35815.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG321880.
GeneTreei ENSGT00760000118926.
HOGENOMi HOG000280688.
HOVERGENi HBG006384.
InParanoidi O15427.
KOi K08180.
OMAi GNFFCIR.
PhylomeDBi O15427.
TreeFami TF313792.

Enzyme and pathway databases

Reactomei REACT_12560. Basigin interactions.
REACT_20515. Proton-coupled monocarboxylate transport.
REACT_2071. Pyruvate metabolism.

Miscellaneous databases

ChiTaRSi SLC16A3. human.
GeneWikii SLC16A3.
GenomeRNAii 9123.
NextBioi 34193.
PROi O15427.
SOURCEi Search...

Gene expression databases

Bgeei O15427.
CleanExi HS_SLC16A3.
ExpressionAtlasi O15427. baseline and differential.
Genevestigatori O15427.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004743. Monocarb_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 1 hit.
TIGRFAMsi TIGR00892. 2A0113. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past."
    Price N.T., Jackson V.N., Halestrap A.P.
    Biochem. J. 329:321-328(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Blood.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Hippocampus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  4. Bienvenut W.V., Lao L., Ryan K.L.
    Submitted (OCT-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-14 AND 429-441, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Cervix carcinoma.
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMOT4_HUMAN
AccessioniPrimary (citable) accession number: O15427
Secondary accession number(s): B3KXG8, Q2M1P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was initially assigned as monocarboxylate transporter 3 (MCT3) (PubMed:9425115). However, it was later shown that it corresponds to monocarboxylate transporter 4 (MCT4).1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3