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O15427

- MOT4_HUMAN

UniProt

O15427 - MOT4_HUMAN

Protein

Monocarboxylate transporter 4

Gene

SLC16A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate By similarity.By similarity

    GO - Molecular functioni

    1. monocarboxylic acid transmembrane transporter activity Source: ProtInc
    2. poly(A) RNA binding Source: UniProtKB
    3. secondary active monocarboxylate transmembrane transporter activity Source: InterPro
    4. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. cellular metabolic process Source: Reactome
    3. leukocyte migration Source: Reactome
    4. monocarboxylic acid transport Source: ProtInc
    5. pyruvate metabolic process Source: Reactome
    6. small molecule metabolic process Source: Reactome
    7. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_12560. Basigin interactions.
    REACT_20515. Proton-coupled monocarboxylate transport.
    REACT_2071. Pyruvate metabolism.

    Protein family/group databases

    TCDBi2.A.1.13.6. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monocarboxylate transporter 4
    Short name:
    MCT 4
    Alternative name(s):
    Solute carrier family 16 member 3
    Gene namesi
    Name:SLC16A3
    Synonyms:MCT4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:10924. SLC16A3.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cytoskeleton Source: HPA
    2. integral component of plasma membrane Source: ProtInc
    3. membrane Source: ProtInc
    4. nuclear membrane Source: HPA
    5. plasma membrane Source: HPA

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35815.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 465464Monocarboxylate transporter 4PRO_0000211394Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei460 – 4601Phosphothreonine4 Publications
    Modified residuei464 – 4641Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO15427.
    PaxDbiO15427.
    PeptideAtlasiO15427.
    PRIDEiO15427.

    PTM databases

    PhosphoSiteiO15427.

    Expressioni

    Tissue specificityi

    Highly expressed in skeletal muscle.

    Gene expression databases

    ArrayExpressiO15427.
    BgeeiO15427.
    CleanExiHS_SLC16A3.
    GenevestigatoriO15427.

    Organism-specific databases

    HPAiCAB017490.
    HPA021451.

    Interactioni

    Protein-protein interaction databases

    BioGridi114571. 1 interaction.
    IntActiO15427. 1 interaction.
    MINTiMINT-4526381.
    STRINGi9606.ENSP00000376150.

    Structurei

    3D structure databases

    ProteinModelPortaliO15427.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1716CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini39 – 6123ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini83 – 842CytoplasmicSequence Analysis
    Topological domaini106 – 1094ExtracellularSequence Analysis
    Topological domaini131 – 14919CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini171 – 1799ExtracellularSequence Analysis
    Topological domaini201 – 22727CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini249 – 26416ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini286 – 2949CytoplasmicSequence Analysis
    Topological domaini316 – 3172ExtracellularSequence Analysis
    Topological domaini339 – 35113CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini373 – 38412ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini406 – 46560CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei18 – 3821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei150 – 17021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei180 – 20021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei228 – 24821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei295 – 31521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei318 – 33821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei352 – 37221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei385 – 40521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG321880.
    HOGENOMiHOG000280688.
    HOVERGENiHBG006384.
    InParanoidiO15427.
    KOiK08180.
    OMAiGNFFCIR.
    PhylomeDBiO15427.
    TreeFamiTF313792.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004743. Monocarb_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00892. 2A0113. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O15427-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGGAVVDEGP TGVKAPDGGW GWAVLFGCFV ITGFSYAFPK AVSVFFKELI    50
    QEFGIGYSDT AWISSILLAM LYGTGPLCSV CVNRFGCRPV MLVGGLFASL 100
    GMVAASFCRS IIQVYLTTGV ITGLGLALNF QPSLIMLNRY FSKRRPMANG 150
    LAAAGSPVFL CALSPLGQLL QDRYGWRGGF LILGGLLLNC CVCAALMRPL 200
    VVTAQPGSGP PRPSRRLLDL SVFRDRGFVL YAVAASVMVL GLFVPPVFVV 250
    SYAKDLGVPD TKAAFLLTIL GFIDIFARPA AGFVAGLGKV RPYSVYLFSF 300
    SMFFNGLADL AGSTAGDYGG LVVFCIFFGI SYGMVGALQF EVLMAIVGTH 350
    KFSSAIGLVL LMEAVAVLVG PPSGGKLLDA THVYMYVFIL AGAEVLTSSL 400
    ILLLGNFFCI RKKPKEPQPE VAAAEEEKLH KPPADSGVDL REVEHFLKAE 450
    PEKNGEVVHT PETSV 465
    Length:465
    Mass (Da):49,469
    Last modified:January 1, 1998 - v1
    Checksum:i7BF6384B0F14D927
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81800 mRNA. Translation: AAC52015.1.
    AK127319 mRNA. Translation: BAG54480.1.
    BC112267 mRNA. Translation: AAI12268.1.
    BC112269 mRNA. Translation: AAI12270.1.
    CCDSiCCDS11804.1.
    RefSeqiNP_001035887.1. NM_001042422.2.
    NP_001035888.1. NM_001042423.2.
    NP_001193879.1. NM_001206950.1.
    NP_001193880.1. NM_001206951.1.
    NP_001193881.1. NM_001206952.1.
    NP_004198.1. NM_004207.3.
    UniGeneiHs.500761.

    Genome annotation databases

    EnsembliENST00000392339; ENSP00000376150; ENSG00000141526.
    ENST00000392341; ENSP00000376152; ENSG00000141526.
    ENST00000581287; ENSP00000463978; ENSG00000141526.
    ENST00000582743; ENSP00000462405; ENSG00000141526.
    GeneIDi9123.
    KEGGihsa:9123.
    UCSCiuc002kea.3. human.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81800 mRNA. Translation: AAC52015.1 .
    AK127319 mRNA. Translation: BAG54480.1 .
    BC112267 mRNA. Translation: AAI12268.1 .
    BC112269 mRNA. Translation: AAI12270.1 .
    CCDSi CCDS11804.1.
    RefSeqi NP_001035887.1. NM_001042422.2.
    NP_001035888.1. NM_001042423.2.
    NP_001193879.1. NM_001206950.1.
    NP_001193880.1. NM_001206951.1.
    NP_001193881.1. NM_001206952.1.
    NP_004198.1. NM_004207.3.
    UniGenei Hs.500761.

    3D structure databases

    ProteinModelPortali O15427.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114571. 1 interaction.
    IntActi O15427. 1 interaction.
    MINTi MINT-4526381.
    STRINGi 9606.ENSP00000376150.

    Chemistry

    ChEMBLi CHEMBL2073663.
    DrugBanki DB00119. Pyruvic acid.

    Protein family/group databases

    TCDBi 2.A.1.13.6. the major facilitator superfamily (mfs).

    PTM databases

    PhosphoSitei O15427.

    Proteomic databases

    MaxQBi O15427.
    PaxDbi O15427.
    PeptideAtlasi O15427.
    PRIDEi O15427.

    Protocols and materials databases

    DNASUi 9123.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000392339 ; ENSP00000376150 ; ENSG00000141526 .
    ENST00000392341 ; ENSP00000376152 ; ENSG00000141526 .
    ENST00000581287 ; ENSP00000463978 ; ENSG00000141526 .
    ENST00000582743 ; ENSP00000462405 ; ENSG00000141526 .
    GeneIDi 9123.
    KEGGi hsa:9123.
    UCSCi uc002kea.3. human.

    Organism-specific databases

    CTDi 9123.
    GeneCardsi GC17P080186.
    HGNCi HGNC:10924. SLC16A3.
    HPAi CAB017490.
    HPA021451.
    MIMi 603877. gene.
    neXtProti NX_O15427.
    PharmGKBi PA35815.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG321880.
    HOGENOMi HOG000280688.
    HOVERGENi HBG006384.
    InParanoidi O15427.
    KOi K08180.
    OMAi GNFFCIR.
    PhylomeDBi O15427.
    TreeFami TF313792.

    Enzyme and pathway databases

    Reactomei REACT_12560. Basigin interactions.
    REACT_20515. Proton-coupled monocarboxylate transport.
    REACT_2071. Pyruvate metabolism.

    Miscellaneous databases

    ChiTaRSi SLC16A3. human.
    GeneWikii SLC16A3.
    GenomeRNAii 9123.
    NextBioi 34193.
    PROi O15427.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O15427.
    Bgeei O15427.
    CleanExi HS_SLC16A3.
    Genevestigatori O15427.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004743. Monocarb_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00892. 2A0113. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past."
      Price N.T., Jackson V.N., Halestrap A.P.
      Biochem. J. 329:321-328(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Blood.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Hippocampus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    4. Bienvenut W.V., Lao L., Ryan K.L.
      Submitted (OCT-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-14 AND 429-441, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Cervix carcinoma.
    5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMOT4_HUMAN
    AccessioniPrimary (citable) accession number: O15427
    Secondary accession number(s): B3KXG8, Q2M1P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Was initially assigned as monocarboxylate transporter 3 (MCT3) (PubMed:9425115). However, it was later shown that it corresponds to monocarboxylate transporter 4 (MCT4).1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3