O15427 (MOT4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monocarboxylate transporter 4
Short name=MCT 4

Alternative name(s):
Solute carrier family 16 member 3

Gene names

Name:	SLC16A3
Synonyms:	MCT4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Highly expressed in skeletal muscle.
Sequence similarities	Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]
Caution	Was initially assigned as monocarboxylate transporter 3 (MCT3) (Ref.1). However, it was later shown that it corresponds to monocarboxylate transporter 4 (MCT4).

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome leukocyte migration Traceable author statement. Source: Reactome pyruvate metabolic process Traceable author statement. Source: Reactome
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: HPA integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc nuclear membrane Inferred from direct assay. Source: HPA
Molecular_function	monocarboxylic acid transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc secondary active monocarboxylate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 465	464	Monocarboxylate transporter 4		PRO_0000211394
Regions
	Topological domain	2 – 17	16	Cytoplasmic Potential
	Transmembrane	18 – 38	21	Helical; Potential
	Topological domain	39 – 61	23	Extracellular Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Topological domain	83 – 84	2	Cytoplasmic Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Topological domain	106 – 109	4	Extracellular Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Topological domain	131 – 149	19	Cytoplasmic Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Topological domain	171 – 179	9	Extracellular Potential
	Transmembrane	180 – 200	21	Helical; Potential
	Topological domain	201 – 227	27	Cytoplasmic Potential
	Transmembrane	228 – 248	21	Helical; Potential
	Topological domain	249 – 264	16	Extracellular Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Topological domain	286 – 294	9	Cytoplasmic Potential
	Transmembrane	295 – 315	21	Helical; Potential
	Topological domain	316 – 317	2	Extracellular Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Topological domain	339 – 351	13	Cytoplasmic Potential
	Transmembrane	352 – 372	21	Helical; Potential
	Topological domain	373 – 384	12	Extracellular Potential
	Transmembrane	385 – 405	21	Helical; Potential
	Topological domain	406 – 465	60	Cytoplasmic Potential
Sites
	Site	2	1	Not acetylated
Amino acid modifications
	Modified residue	460	1	Phosphothreonine Ref.5 Ref.6 Ref.7 Ref.8
	Modified residue	464	1	Phosphoserine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	O15427 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7BF6384B0F14D927	FASTA	465	49,469
	10 20 30 40 50 60 MGGAVVDEGP TGVKAPDGGW GWAVLFGCFV ITGFSYAFPK AVSVFFKELI QEFGIGYSDT 70 80 90 100 110 120 AWISSILLAM LYGTGPLCSV CVNRFGCRPV MLVGGLFASL GMVAASFCRS IIQVYLTTGV 130 140 150 160 170 180 ITGLGLALNF QPSLIMLNRY FSKRRPMANG LAAAGSPVFL CALSPLGQLL QDRYGWRGGF 190 200 210 220 230 240 LILGGLLLNC CVCAALMRPL VVTAQPGSGP PRPSRRLLDL SVFRDRGFVL YAVAASVMVL 250 260 270 280 290 300 GLFVPPVFVV SYAKDLGVPD TKAAFLLTIL GFIDIFARPA AGFVAGLGKV RPYSVYLFSF 310 320 330 340 350 360 SMFFNGLADL AGSTAGDYGG LVVFCIFFGI SYGMVGALQF EVLMAIVGTH KFSSAIGLVL 370 380 390 400 410 420 LMEAVAVLVG PPSGGKLLDA THVYMYVFIL AGAEVLTSSL ILLLGNFFCI RKKPKEPQPE 430 440 450 460 VAAAEEEKLH KPPADSGVDL REVEHFLKAE PEKNGEVVHT PETSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past." Price N.T., Jackson V.N., Halestrap A.P. Biochem. J. 329:321-328(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[4]	Bienvenut W.V., Lao L., Ryan K.L. Submitted (OCT-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-14 AND 429-441, CLEAVAGE OF INITIATOR METHIONINE, LACK OF N-TERMINAL ACETYLATION, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460 AND SER-464, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-460 AND SER-464, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	U81800 mRNA. Translation: AAC52015.1. AK127319 mRNA. Translation: BAG54480.1. BC112267 mRNA. Translation: AAI12268.1. BC112269 mRNA. Translation: AAI12270.1.
IPI	IPI00006666.
RefSeq	NP_001035887.1. NM_001042422.2. NP_001035888.1. NM_001042423.2. NP_001193879.1. NM_001206950.1. NP_001193880.1. NM_001206951.1. NP_001193881.1. NM_001206952.1. NP_004198.1. NM_004207.3.
UniGene	Hs.500761.
3D structure databases
ProteinModelPortal	O15427.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4526381.
STRING	9606.ENSP00000376150.
Protein family/group databases
TCDB	2.A.1.13.6. major facilitator superfamily (MFS).
PTM databases
PhosphoSite	O15427.
Proteomic databases
PaxDb	O15427.
PeptideAtlas	O15427.
PRIDE	O15427.
Protocols and materials databases
DNASU	9123.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000392339; ENSP00000376150; ENSG00000141526. ENST00000392341; ENSP00000376152; ENSG00000141526. ENST00000581287; ENSP00000463978; ENSG00000141526. ENST00000582743; ENSP00000462405; ENSG00000141526.
GeneID	9123.
KEGG	hsa:9123.
UCSC	uc002kea.3. human.
Organism-specific databases
CTD	9123.
GeneCards	GC17P080186.
HGNC	HGNC:10924. SLC16A3.
HPA	CAB017490. HPA021451.
MIM	603877. gene.
neXtProt	NX_O15427.
PharmGKB	PA35815.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG321880.
HOGENOM	HOG000280688.
HOVERGEN	HBG006384.
InParanoid	O15427.
KO	K08180.
OMA	WGCFVIT.
OrthoDB	EOG4TTGJ4.
PhylomeDB	O15427.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_15518. Transmembrane transport of small molecules. REACT_20633. Bile salt and organic anion SLC transporters. REACT_604. Hemostasis.
Gene expression databases
Bgee	O15427.
CleanEx	HS_SLC16A3.
Genevestigator	O15427.
GermOnline	ENSG00000141526. Homo sapiens.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004743. Monocarb_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00892. 2A0113. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC16A3. human.
DrugBank	DB00119. Pyruvic acid.
GenomeRNAi	9123.
NextBio	34193.
SOURCE	Search...

Entry information

Entry name

MOT4_HUMAN

Accession

Primary (citable) accession number: O15427
Secondary accession number(s): B3KXG8, Q2M1P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.