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O15399 (NMDE4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate receptor ionotropic, NMDA 2D

Short name=GluN2D
Alternative name(s):
EB11
Glutamate [NMDA] receptor subunit epsilon-4
N-methyl D-aspartate receptor subtype 2D
Short name=NMDAR2D
Short name=NR2D
Gene names
Name:GRIN2D
Synonyms:GluN2D, NMDAR2D
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1336 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Subunit structure

Interacts with PDZ domains of INADL and DLG4 By similarity. Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B).

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   LigandCalcium
Magnesium
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadult locomotory behavior

Inferred from electronic annotation. Source: Ensembl

ion transmembrane transport

Inferred from electronic annotation. Source: GOC

ionotropic glutamate receptor signaling pathway

Inferred from electronic annotation. Source: GOC

regulation of sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

startle response

Inferred from electronic annotation. Source: Ensembl

synaptic transmission

Traceable author statement. Source: Reactome

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionN-methyl-D-aspartate selective glutamate receptor activity

Inferred from electronic annotation. Source: Ensembl

extracellular-glutamate-gated ion channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg3Q629362EBI-1754030,EBI-349596From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 13361309Glutamate receptor ionotropic, NMDA 2D
PRO_0000011583

Regions

Topological domain28 – 583556Extracellular Potential
Transmembrane584 – 60421Helical; Potential
Topological domain605 – 65652Cytoplasmic Potential
Transmembrane657 – 67721Helical; Potential
Topological domain678 – 844167Extracellular Potential
Transmembrane845 – 86521Helical; Potential
Topological domain866 – 1336471Cytoplasmic Potential
Motif1334 – 13363PDZ-binding By similarity
Compositional bias281 – 2866Poly-Gly
Compositional bias908 – 9169Poly-Pro
Compositional bias1035 – 10406Poly-Ala
Compositional bias1209 – 12135Poly-Pro
Compositional bias1244 – 12474Poly-Ala

Sites

Site6421Functional determinant of NMDA receptors By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential
Glycosylation3661N-linked (GlcNAc...) Potential
Glycosylation4671N-linked (GlcNAc...) Potential
Glycosylation5691N-linked (GlcNAc...) Potential
Disulfide bond455 ↔ 483 By similarity
Disulfide bond462 ↔ 484 By similarity
Disulfide bond773 ↔ 828 By similarity

Natural variations

Natural variant1401P → S in a breast cancer sample; somatic mutation. Ref.3
VAR_035698
Natural variant2861G → R in a breast cancer sample; somatic mutation. Ref.3
VAR_035699
Natural variant5271E → G in a breast cancer sample; somatic mutation. Ref.3
VAR_035700

Experimental info

Sequence conflict9241R → G in AAC15910. Ref.1
Sequence conflict10051P → A in AAC15910. Ref.1
Sequence conflict10971R → C in AAC15910. Ref.1
Sequence conflict11301E → D in AAC15910. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O15399 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 0DB7559056AE4593

FASTA1,336143,752
        10         20         30         40         50         60 
MRGAGGPRGP RGPAKMLLLL ALACASPFPE EAPGPGGAGG PGGGLGGARP LNVALVFSGP 

        70         80         90        100        110        120 
AYAAEAARLG PAVAAAVRSP GLDVRPVALV LNGSDPRSLV LQLCDLLSGL RVHGVVFEDD 

       130        140        150        160        170        180 
SRAPAVAPIL DFLSAQTSLP IVAVHGGAAL VLTPKEKGST FLQLGSSTEQ QLQVIFEVLE 

       190        200        210        220        230        240 
EYDWTSFVAV TTRAPGHRAF LSYIEVLTDG SLVGWEHRGA LTLDPGAGEA VLSAQLRSVS 

       250        260        270        280        290        300 
AQIRLLFCAR EEAEPVFRAA EEAGLTGSGY VWFMVGPQLA GGGGSGAPGE PPLLPGGAPL 

       310        320        330        340        350        360 
PAGLFAVRSA GWRDDLARRV AAGVAVVARG AQALLRDYGF LPELGHDCRA QNRTHRGESL 

       370        380        390        400        410        420 
HRYFMNITWD NRDYSFNEDG FLVNPSLVVI SLTRDRTWEV VGSWEQQTLR LKYPLWSRYG 

       430        440        450        460        470        480 
RFLQPVDDTQ HLTVATLEER PFVIVEPADP ISGTCIRDSV PCRSQLNRTH SPPPDAPRPE 

       490        500        510        520        530        540 
KRCCKGFCID ILKRLAHTIG FSYDLYLVTN GKHGKKIDGV WNGMIGEVFY QRADMAIGSL 

       550        560        570        580        590        600 
TINEERSEIV DFSVPFVETG ISVMVARSNG TVSPSAFLEP YSPAVWVMMF VMCLTVVAVT 

       610        620        630        640        650        660 
VFIFEYLSPV GYNRSLATGK RPGGSTFTIG KSIWLLWALV FNNSVPVENP RGTTSKIMVL 

       670        680        690        700        710        720 
VWAFFAVIFL ASYTANLAAF MIQEEYVDTV SGLSDRKFQR PQEQYPPLKF GTVPNGSTEK 

       730        740        750        760        770        780 
NIRSNYPDMH SYMVRYNQPR VEEALTQLKA GKLDAFIYDA AVLNYMARKD EGCKLVTIGS 

       790        800        810        820        830        840 
GKVFATTGYG IALHKGSRWK RPIDLALLQF LGDDEIEMLE RLWLSGICHN DKIEVMSSKL 

       850        860        870        880        890        900 
DIDNMAGVFY MLLVAMGLSL LVFAWEHLVY WRLRHCLGPT HRMDFLLAFS RGMYSCCSAE 

       910        920        930        940        950        960 
AAPPPAKPPP PPQPLPSPAY PAPRPAPGPA PFVPRERASV DRWRRTKGAG PPGGAGLADG 

       970        980        990       1000       1010       1020 
FHRYYGPIEP QGLGLGLGEA RAAPRGAAGR PLSPPAAQPP QKPPPSYFAI VRDKEPAEPP 

      1030       1040       1050       1060       1070       1080 
AGAFPGFPSP PAPPAAAATA VGPPLCRLAF EDESPPAPAR WPRSDPESQP LLGPGAGGAG 

      1090       1100       1110       1120       1130       1140 
GTGGAGGGAP AAPPPCRAAP PPCPYLDLEP SPSDSEDSES LGGASLGGLE PWWFADFPYP 

      1150       1160       1170       1180       1190       1200 
YAERLGPPPG RYWSVDKLGG WRAGSWDYLP PRSGPAAWHC RHCASLELLP PPRHLSCSHD 

      1210       1220       1230       1240       1250       1260 
GLDGGWWAPP PPPWAAGPLP RRRARCGCPR SHPHRPRASH RTPAAAAPHH HRHRRAAGGW 

      1270       1280       1290       1300       1310       1320 
DLPPPAPTSR SLEDLSSCPR AAPARRLTGP SRHARRCPHA AHWGPPLPTA SHRRHRGGDL 

      1330 
GTRRGSAHFS SLESEV 

« Hide

References

« Hide 'large scale' references
[1]"Functional characterization of human N-methyl-D-aspartate subtype 1A/2D receptors."
Hess S.D., Daggett L.P., Deal C., Lu C.-C., Johnson E.C., Velicelebi G.
J. Neurochem. 70:1269-1279(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal brain.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-140; ARG-286 AND GLY-527.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77783 mRNA. Translation: AAC15910.1.
AC008403 Genomic DNA. No translation available.
AC011527 Genomic DNA. No translation available.
RefSeqNP_000827.2. NM_000836.2.
UniGeneHs.445015.

3D structure databases

ProteinModelPortalO15399.
SMRO15399. Positions 50-418, 429-829.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109163. 4 interactions.
IntActO15399. 4 interactions.
MINTMINT-130143.
STRING9606.ENSP00000263269.

Chemistry

BindingDBO15399.
ChEMBLCHEMBL2094124.
DrugBankDB00142. L-Glutamic Acid.
DB00836. Loperamide.
DB01173. Orphenadrine.
GuidetoPHARMACOLOGY459.

PTM databases

PhosphoSiteO15399.

Proteomic databases

PaxDbO15399.
PRIDEO15399.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263269; ENSP00000263269; ENSG00000105464.
GeneID2906.
KEGGhsa:2906.
UCSCuc002pjc.4. human.

Organism-specific databases

CTD2906.
GeneCardsGC19P048898.
H-InvDBHIX0040164.
HGNCHGNC:4588. GRIN2D.
HPAHPA038269.
MIM602717. gene.
neXtProtNX_O15399.
PharmGKBPA28982.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG282132.
HOGENOMHOG000113803.
HOVERGENHBG052637.
InParanoidO15399.
KOK05212.
OMAWDYLPPR.
OrthoDBEOG72ZCD1.
PhylomeDBO15399.
TreeFamTF314731.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.
SignaLinkO15399.

Gene expression databases

BgeeO15399.
CleanExHS_GRIN2D.
GenevestigatorO15399.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
ProtoNetSearch...

Other

GeneWikiGRIN2D.
GenomeRNAi2906.
NextBio11507.
PROO15399.
SOURCESearch...

Entry information

Entry nameNMDE4_HUMAN
AccessionPrimary (citable) accession number: O15399
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 5, 2009
Last modified: March 19, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM