O15397 (IPO8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Importin-8
Short name=Imp8

Alternative name(s):
Ran-binding protein 8
Short name=RanBP8

Gene names

Name:	IPO8
Synonyms:	RANBP8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1037 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19. Ref.1 Ref.4
Subunit structure	Forms a heterodimer with KPNB1. Interacts with SRP19. Binds directly to nuclear pore complexes. Ref.1 Ref.4
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the importin beta family. Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore Inferred from electronic annotation. Source: InterPro
Molecular_function	Ran GTPase binding Traceable author statement Ref.1. Source: ProtInc protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF2C1	Q9UL18	2	EBI-358808,EBI-527363
EIF2C2	Q9UKV8	3	EBI-358808,EBI-528269
EIF2C3	Q9H9G7	5	EBI-358808,EBI-2267883
EIF2C4	Q9HCK5	3	EBI-358808,EBI-2269696

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O15397-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O15397-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-213: MDLNRIIQAL...ILKIFYALVQ → MESLTLK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1037	1037	Importin-8		PRO_0000120752
Regions
	Domain	22 – 102	81	Importin N-terminal
	Compositional bias	923 – 928	6	Poly-Glu
Amino acid modifications
	Modified residue	902	1	Phosphoserine Ref.6 Ref.7 Ref.9
	Modified residue	903	1	Phosphoserine Ref.6 Ref.7 Ref.9
Natural variations
	Alternative sequence	1 – 213	213	MDLNR…YALVQ → MESLTLK in isoform 2.		VSP_042574
	Natural variant	6	1	I → F. Ref.1 Corresponds to variant rs1054423 [ dbSNP | Ensembl ].		VAR_055118
	Natural variant	640	1	I → V. Corresponds to variant rs34119940 [ dbSNP | Ensembl ].		VAR_055119
Experimental info
	Sequence conflict	91	1	Q → R in AAB67052. Ref.1
	Sequence conflict	279	1	P → A in AAB67052. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 3D7195FD57D7D9AF	FASTA	1,037	119,938
	10 20 30 40 50 60 MDLNRIIQAL KGTIDPKLRI AAENELNQSY KIINFAPSLL RIIVSDHVEF PVRQAAAIYL 70 80 90 100 110 120 KNMVTQYWPD REPPPGEAIF PFNIHENDRQ QIRDNIVEGI IRSPDLVRVQ LTMCLRAIIK 130 140 150 160 170 180 HDFPGHWPGV VDKIDYYLQS QSSASWLGSL LCLYQLVKTY EYKKAEEREP LIIAMQIFLP 190 200 210 220 230 240 RIQQQIVQLL PDSSYYSVLL QKQILKIFYA LVQYALPLQL VNNQTMTTWM EIFRTIIDRT 250 260 270 280 290 300 VPPETLHIDE DDRPELVWWK CKKWALHIVA RLFERYGSPG NVTKEYFEFS EFFLKTYAVG 310 320 330 340 350 360 IQQVLLKILD QYRQKEYVAP RVLQQAFNYL NQGVVHSITW KQMKPHIQNI SEDVIFSVMC 370 380 390 400 410 420 YKDEDEELWQ EDPYEYIRMK FDIFEDYASP TTAAQTLLYT AAKKRKEVLP KMMAFCYQIL 430 440 450 460 470 480 TDPNFDPRKK DGALHVIGSL AEILLKKSLF KDQMELFLQN HVFPLLLSNL GYLRARSCWV 490 500 510 520 530 540 LHAFSSLKFH NELNLRNAVE LAKKSLIEDK EMPVKVEAAL ALQSLISNQI QAKEYMKPHV 550 560 570 580 590 600 RPIMQELLHI VRETENDDVT NVIQKMICEY SQEVASIAVD MTQHLAEIFG KVLQSDEYEE 610 620 630 640 650 660 VEDKTVMAMG ILHTIDTILT VVEDHKEITQ QLENICLRII DLVLQKHVIE FYEEILSLAY 670 680 690 700 710 720 SLTCHSISPQ MWQLLGILYE VFQQDCFEYF TDMMPLLHNY VTIDTDTLLS NAKHLEILFT 730 740 750 760 770 780 MCRKVLCGDA GEDAECHAAK LLEVIILQCK GRGIDQCIPL FVQLVLERLT RGVKTSELRT 790 800 810 820 830 840 MCLQVAIAAL YYNPDLLLHT LERIQLPHNP GPITVQFINQ WMNDTDCFLG HHDRKMCIIG 850 860 870 880 890 900 LSILLELQNR PPAVDAVVGQ IVPSILFLFL GLKQVCATRQ LVNREDRSKA EKADMEENEE 910 920 930 940 950 960 ISSDEEETNV TAQAMQSNNG RGEDEEEEDD DWDEEVLEET ALEGFSTPLD LDNSVDEYQF 970 980 990 1000 1010 1020 FTQALITVQS RDAAWYQLLM APLSEDQRTA LQEVYTLAEH RRTVAEAKKK IEQQGGFTFE 1030 NKGVLSAFNF GTVPSNN
	Isoform 2 [UniParc]. Checksum: 80607ACB36848EEB	FASTA	831	95,979
	10 20 30 40 50 60 MESLTLKYAL PLQLVNNQTM TTWMEIFRTI IDRTVPPETL HIDEDDRPEL VWWKCKKWAL 70 80 90 100 110 120 HIVARLFERY GSPGNVTKEY FEFSEFFLKT YAVGIQQVLL KILDQYRQKE YVAPRVLQQA 130 140 150 160 170 180 FNYLNQGVVH SITWKQMKPH IQNISEDVIF SVMCYKDEDE ELWQEDPYEY IRMKFDIFED 190 200 210 220 230 240 YASPTTAAQT LLYTAAKKRK EVLPKMMAFC YQILTDPNFD PRKKDGALHV IGSLAEILLK 250 260 270 280 290 300 KSLFKDQMEL FLQNHVFPLL LSNLGYLRAR SCWVLHAFSS LKFHNELNLR NAVELAKKSL 310 320 330 340 350 360 IEDKEMPVKV EAALALQSLI SNQIQAKEYM KPHVRPIMQE LLHIVRETEN DDVTNVIQKM 370 380 390 400 410 420 ICEYSQEVAS IAVDMTQHLA EIFGKVLQSD EYEEVEDKTV MAMGILHTID TILTVVEDHK 430 440 450 460 470 480 EITQQLENIC LRIIDLVLQK HVIEFYEEIL SLAYSLTCHS ISPQMWQLLG ILYEVFQQDC 490 500 510 520 530 540 FEYFTDMMPL LHNYVTIDTD TLLSNAKHLE ILFTMCRKVL CGDAGEDAEC HAAKLLEVII 550 560 570 580 590 600 LQCKGRGIDQ CIPLFVQLVL ERLTRGVKTS ELRTMCLQVA IAALYYNPDL LLHTLERIQL 610 620 630 640 650 660 PHNPGPITVQ FINQWMNDTD CFLGHHDRKM CIIGLSILLE LQNRPPAVDA VVGQIVPSIL 670 680 690 700 710 720 FLFLGLKQVC ATRQLVNRED RSKAEKADME ENEEISSDEE ETNVTAQAMQ SNNGRGEDEE 730 740 750 760 770 780 EEDDDWDEEV LEETALEGFS TPLDLDNSVD EYQFFTQALI TVQSRDAAWY QLLMAPLSED 790 800 810 820 830 QRTALQEVYT LAEHRRTVAE AKKKIEQQGG FTFENKGVLS AFNFGTVPSN N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel class of RanGTP binding proteins." Goerlich D., Dabrowski M., Bischoff F.R., Kutay U., Bork P., Hartmann E., Prehn S., Izaurralde E. J. Cell Biol. 138:65-80(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH KPNB1, VARIANT PHE-6.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin." Dean K.A., von Ahsen O., Goerlich D., Fried H.M. J. Cell Sci. 114:3479-3485(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SRP19.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-902 AND SER-903, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-902 AND SER-903, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-902 AND SER-903, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77494 mRNA. Translation: AAB67052.1. AK302260 mRNA. Translation: BAH13659.1. AC012673 Genomic DNA. No translation available. AC023426 Genomic DNA. No translation available.
IPI	IPI00007401. IPI00793997.
RefSeq	NP_001177924.1. NM_001190995.1. NP_006381.2. NM_006390.3.
UniGene	Hs.505136.
3D structure databases
ProteinModelPortal	O15397.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15397. 10 interactions.
MINT	MINT-1150774.
STRING	9606.ENSP00000256079.
PTM databases
PhosphoSite	O15397.
Proteomic databases
PaxDb	O15397.
PRIDE	O15397.
Protocols and materials databases
DNASU	10526.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000256079; ENSP00000256079; ENSG00000133704. ENST00000544829; ENSP00000444520; ENSG00000133704.
GeneID	10526.
KEGG	hsa:10526.
UCSC	uc001rjd.3. human. uc001rje.1. human.
Organism-specific databases
CTD	10526.
GeneCards	GC12M030682.
HGNC	HGNC:9853. IPO8.
HPA	HPA018439. HPA023765.
MIM	605600. gene.
neXtProt	NX_O15397.
PharmGKB	PA34214.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5656.
HOGENOM	HOG000006586.
HOVERGEN	HBG006824.
InParanoid	O15397.
OMA	KYDFPGH.
OrthoDB	EOG4MKNFJ.
PhylomeDB	O15397.
Gene expression databases
ArrayExpress	O15397.
Bgee	O15397.
CleanEx	HS_IPO8.
Genevestigator	O15397.
GermOnline	ENSG00000133704. Homo sapiens.
Family and domain databases
Gene3D	1.25.10.10. 2 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR013713. Exportin/Importin_Cse1-like. IPR001494. Importin-beta_N. [Graphical view]
Pfam	PF08506. Cse1. 1 hit. PF03810. IBN_N. 1 hit. [Graphical view]
SMART	SM00913. IBN_N. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50166. IMPORTIN_B_NT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10526.
NextBio	39934.
SOURCE	Search...

Entry information

Entry name

IPO8_HUMAN

Accession

Primary (citable) accession number: O15397
Secondary accession number(s): B7Z7M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.