Reviewed, UniProtKB/Swiss-Prot O15394 (NCAM2_HUMAN)

Names and origin

Protein names

Recommended name:
    Neural cell adhesion molecule 2
      Short name=N-CAM 2

Gene names

Name:	NCAM2
Synonyms:	NCAM21

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed most strongly in adult and fetal brain.
Sequence similarities	Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	neuron adhesion Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Ref.1 Traceable author statement. Source: ProtInc plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 837

818

Neural cell adhesion molecule 2

PRO_0000015018

Regions

Topological domain

20 – 697

678

Extracellular Potential

Transmembrane

698 – 718

21

Potential

Topological domain

719 – 837

119

Cytoplasmic Potential

Domain

21 – 108

88

Ig-like C2-type 1

Domain

113 – 202

90

Ig-like C2-type 2

Domain

208 – 297

90

Ig-like C2-type 3

Domain

302 – 396

95

Ig-like C2-type 4

Domain

401 – 491

91

Ig-like C2-type 5

Domain

496 – 588

93

Fibronectin type-III 1

Domain

590 – 684

95

Fibronectin type-III 2

Amino acid modifications

Modified residue

780

1

Phosphothreonine By similarity

Glycosylation

177

1

N-linked (GlcNAc...) Potential

Glycosylation

219

1

N-linked (GlcNAc...) Potential

Glycosylation

309

1

N-linked (GlcNAc...) Potential

Glycosylation

406

1

N-linked (GlcNAc...) Potential

Glycosylation

419

1

N-linked (GlcNAc...) Potential

Glycosylation

445

1

N-linked (GlcNAc...) Ref.4

Glycosylation

474

1

N-linked (GlcNAc...) Potential

Glycosylation

562

1

N-linked (GlcNAc...) Ref.4

Disulfide bond

42 ↔ 93

Probable

Disulfide bond

136 ↔ 186

Probable

Disulfide bond

232 ↔ 281

Probable

Disulfide bond

322 ↔ 380

Probable

Disulfide bond

422 ↔ 475

Probable

Natural variations

Natural variant

347

1

D → N: dbSNP rs35654962.

VAR_047897

Natural variant

350

1

L → P: dbSNP rs232518. Ref.1

VAR_047898

Experimental info

Sequence conflict

49

1

E → R in AAB80803. Ref.1

Sequence conflict

72

1

E → G in AAB80803. Ref.1

Sequence conflict

163

1

F → L in AAB80803. Ref.1

Sequence conflict

374

1

D → G in AAB80803. Ref.1

Sequence conflict

662 – 667

6

YEVQIT → MKFRLP in AAB80803. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

837

Helix Strand Turn

Details...

Beta strand

500 – 507

8

Beta strand

512 – 517

6

Beta strand

529 – 538

10

Beta strand

543 – 546

4

Beta strand

548 – 550

3

Beta strand

552 – 556

5

Beta strand

564 – 573

10

Beta strand

576 – 580

5

Beta strand

584 – 587

4

Sequences

	Sequence		Length	Mass (Da)
	O15394-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 878EC1110562B3F3	FASTA	837	93,046
	10 20 30 40 50 60 MSLLLSFYLL GLLVSSGQAL LQVTISLSKV ELSVGESKFF TCTAIGEPES IDWYNPQGEK 70 80 90 100 110 120 IISTQRVVVQ KEGVRSRLTI YNANIEDAGI YRCQATDAKG QTQEATVVLE IYQKLTFREV 130 140 150 160 170 180 VSPQEFKQGE DAEVVCRVSS SPAPAVSWLY HNEEVTTISD NRFAMLANNN LQILNINKSD 190 200 210 220 230 240 EGIYRCEGRV EARGEIDFRD IIVIVNVPPA ISMPQKSFNA TAERGEEMTF SCRASGSPEP 250 260 270 280 290 300 AISWFRNGKL IEENEKYILK GSNTELTVRN IINSDGGPYV CRATNKAGED EKQAFLQVFV 310 320 330 340 350 360 QPHIIQLKNE TTYENGQVTL VCDAEGEPIP EITWKRAVDG FTFTEGDKSL DGRIEVKGQH 370 380 390 400 410 420 GSSSLHIKDV KLSDSGRYDC EAASRIGGHQ KSMYLDIEYA PKFISNQTIY YSWEGNPINI 430 440 450 460 470 480 SCDVKSNPPA SIHWRRDKLV LPAKNTTNLK TYSTGRKMIL EIAPTSDNDF GRYNCTATNH 490 500 510 520 530 540 IGTRFQEYIL ALADVPSSPY GVKIIELSQT TAKVSFNKPD SHGGVPIHHY QVDVKEVASE 550 560 570 580 590 600 IWKIVRSHGV QTMVVLNNLE PNTTYEIRVA AVNGKGQGDY SKIEIFQTLP VREPSPPSIH 610 620 630 640 650 660 GQPSSGKSFK LSITKQDDGG APILEYIVKY RSKDKEDQWL EKKVQGNKDH IILEHLQWTM 670 680 690 700 710 720 GYEVQITAAN RLGYSEPTVY EFSMPPKPNI IKDTLFNGLG LGAVIGLGVA ALLLILVVTD 730 740 750 760 770 780 VSCFFIRQCG LLMCITRRMC GKKSGSSGKS KELEEGKAAY LKDGSKEPIV EMRTEDERVT 790 800 810 820 830 NHEDGSPVNE PNETTPLTEP EKLPLKEEDG KEALNPETIE IKVSNDIIQS KEDDSKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a novel human neural cell adhesion molecule gene (NCAM2) that maps to chromosome region 21q21 and is potentially involved in Down syndrome." Paoloni-Giacobino A., Chen H., Antonarakis S.E. Genomics 43:43-51(1997) [PubMed: 9226371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-350. Tissue: Brain.
[2]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed: 12754519] [Abstract] Cited for: GLYCOSYLATION AT ASN-445 AND ASN-562.
[5]	"Solution structure of the fibronectin type-III domain of human neural cell adhesion molecule 2." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 486-591.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U75330 mRNA. Translation: AAB80803.1.
AP001138 Genomic DNA. No translation available.
BC052946 mRNA. Translation: AAH52946.1.

IPI

IPI00376427.

RefSeq

NP_004531.2.

UniGene

Hs.473450

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DOC	NMR	-	A	486-591	[»]
2V44	X-ray	1.77	A	19-207	[»]
2V5T	X-ray	2.00	A	115-301	[»]
2VA4	X-ray	1.90	A	209-398	[»]
2VAJ	X-ray	2.70	A	21-113	[»]

SMR

O15394. Positions 482-595.

ModBase

Search...

PTM databases

PhosphoSite

O15394.

Proteomic databases

PRIDE

O15394.

Genome annotation databases

Ensembl

ENSG00000154654. Homo sapiens. [Contig view]

GeneID

4685.

KEGG

hsa:4685.

Organism-specific databases

GeneCards

GC21P021292.

H-InvDB

HIX0027799.

HGNC

HGNC:7657. NCAM2.

MIM

602040. gene.

PharmGKB

PA31460.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O15394.

HOVERGEN

O15394.

OMA

O15394. TWKIVRS.

Gene expression databases

ArrayExpress

O15394.

Bgee

O15394.

CleanEx

HS_NCAM2.

GermOnline

ENSG00000154654. Homo sapiens.

Family and domain databases

InterPro

IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR013151. Ig.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR009138. Neural_cell_adh.
[Graphical view]

Gene3D

G3DSA:2.60.40.30. FN_III-like. 2 hits.
G3DSA:2.60.40.10. Ig-like_fold. 5 hits.

Pfam

PF00041. fn3. 2 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 3 hits.
[Graphical view]

PRINTS

PR01838. NCAMFAMILY.

SMART

SM00060. FN3. 2 hits.
SM00408. IGc2. 5 hits.
[Graphical view]

PROSITE

PS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

18068.

SOURCE

Search...

Entry information

Entry name

NCAM2_HUMAN

Accession

Primary (citable) accession number: O15394
Secondary accession number(s): A8MQ06, Q7Z7F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)