Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sialic acid-binding Ig-like lectin 5

Gene

SIGLEC5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191Sialic acid
Binding sitei127 – 1271Sialic acid
Binding sitei129 – 1291Sialic acid

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 5
Short name:
Siglec-5
Alternative name(s):
CD33 antigen-like 2
Obesity-binding protein 2
Short name:
OB-BP2
Short name:
OB-binding protein 2
CD_antigen: CD170
Gene namesi
Name:SIGLEC5
Synonyms:CD33L2, OBBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10874. SIGLEC5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 441425ExtracellularSequence AnalysisAdd
BLAST
Transmembranei442 – 46221HelicalSequence AnalysisAdd
BLAST
Topological domaini463 – 55189CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35775.

Polymorphism and mutation databases

BioMutaiSIGLEC5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 551535Sialic acid-binding Ig-like lectin 5PRO_0000014944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 170PROSITE-ProRule annotation1 Publication
Disulfide bondi41 ↔ 101PROSITE-ProRule annotation1 Publication
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi164 ↔ 213PROSITE-ProRule annotation1 Publication
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi269 ↔ 314PROSITE-ProRule annotation
Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi384 – 3841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO15389.

PTM databases

PhosphoSiteiO15389.

Expressioni

Tissue specificityi

Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

Gene expression databases

BgeeiO15389.
CleanExiHS_SIGLEC5.
GenevisibleiO15389. HS.

Organism-specific databases

HPAiCAB024900.
HPA009085.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIP13Q156454EBI-750381,EBI-358993
VAC14Q08AM63EBI-750381,EBI-2107455

Protein-protein interaction databases

BioGridi114308. 3 interactions.
IntActiO15389. 3 interactions.
MINTiMINT-1445881.
STRINGi9606.ENSP00000455510.

Structurei

Secondary structure

1
551
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 243Combined sources
Beta strandi27 – 326Combined sources
Beta strandi37 – 393Combined sources
Beta strandi42 – 443Combined sources
Beta strandi56 – 627Combined sources
Helixi67 – 693Combined sources
Beta strandi72 – 765Combined sources
Beta strandi78 – 803Combined sources
Turni84 – 896Combined sources
Beta strandi90 – 923Combined sources
Helixi96 – 983Combined sources
Beta strandi103 – 1053Combined sources
Helixi110 – 1123Combined sources
Beta strandi114 – 1229Combined sources
Turni123 – 1253Combined sources
Beta strandi126 – 1294Combined sources
Beta strandi135 – 1406Combined sources
Beta strandi147 – 1493Combined sources
Beta strandi160 – 1656Combined sources
Beta strandi171 – 1744Combined sources
Beta strandi177 – 1837Combined sources
Beta strandi186 – 1883Combined sources
Turni190 – 1934Combined sources
Beta strandi195 – 2006Combined sources
Turni204 – 2085Combined sources
Beta strandi210 – 2167Combined sources
Beta strandi225 – 2295Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZG1X-ray2.70A20-233[»]
2ZG2X-ray2.85A20-233[»]
2ZG3X-ray3.00A20-233[»]
ProteinModelPortaliO15389.
SMRiO15389. Positions 20-233, 259-335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15389.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 136118Ig-like V-typeAdd
BLAST
Domaini146 – 22984Ig-like C2-type 1Add
BLAST
Domaini236 – 33095Ig-like C2-type 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi518 – 5236ITIM motif
Motifi542 – 5476SLAM-like motif

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
HOVERGENiHBG036161.
InParanoidiO15389.
KOiK06549.
OMAiPEKMDDE.
OrthoDBiEOG7JDQXC.
PhylomeDBiO15389.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O15389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPLLLLPLL WGGSLQEKPV YELQVQKSVT VQEGLCVLVP CSFSYPWRSW
60 70 80 90 100
YSSPPLYVYW FRDGEIPYYA EVVATNNPDR RVKPETQGRF RLLGDVQKKN
110 120 130 140 150
CSLSIGDARM EDTGSYFFRV ERGRDVKYSY QQNKLNLEVT ALIEKPDIHF
160 170 180 190 200
LEPLESGRPT RLSCSLPGSC EAGPPLTFSW TGNALSPLDP ETTRSSELTL
210 220 230 240 250
TPRPEDHGTN LTCQMKRQGA QVTTERTVQL NVSYAPQTIT IFRNGIALEI
260 270 280 290 300
LQNTSYLPVL EGQALRLLCD APSNPPAHLS WFQGSPALNA TPISNTGILE
310 320 330 340 350
LRRVRSAEEG GFTCRAQHPL GFLQIFLNLS VYSLPQLLGP SCSWEAEGLH
360 370 380 390 400
CRCSFRARPA PSLCWRLEEK PLEGNSSQGS FKVNSSSAGP WANSSLILHG
410 420 430 440 450
GLSSDLKVSC KAWNIYGSQS GSVLLLQGRS NLGTGVVPAA LGGAGVMALL
460 470 480 490 500
CICLCLIFFL IVKARRKQAA GRPEKMDDED PIMGTITSGS RKKPWPDSPG
510 520 530 540 550
DQASPPGDAP PLEEQKELHY ASLSFSEMKS REPKDQEAPS TTEYSEIKTS

K
Length:551
Mass (Da):60,715
Last modified:January 1, 1998 - v1
Checksum:i2FEA2B6B341EFEAF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3091E → K in AAD50978 (PubMed:9731071).Curated
Sequence conflicti388 – 3881A → P in AAD50978 (PubMed:9731071).Curated
Sequence conflicti403 – 4031S → N in AAD50978 (PubMed:9731071).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721V → A.
Corresponds to variant rs1973019 [ dbSNP | Ensembl ].
VAR_014249
Natural varianti215 – 2151M → V.
Corresponds to variant rs1807124 [ dbSNP | Ensembl ].
VAR_014250
Natural varianti322 – 3221F → S.
Corresponds to variant rs2278831 [ dbSNP | Ensembl ].
VAR_014251
Natural varianti358 – 3581R → W.1 Publication
Corresponds to variant rs8108074 [ dbSNP | Ensembl ].
VAR_049929
Natural varianti499 – 4991P → A.1 Publication
Corresponds to variant rs3829655 [ dbSNP | Ensembl ].
VAR_020087

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170484 mRNA. Translation: AAD50978.1.
U71383 mRNA. Translation: AAB70703.1.
AC018755 Genomic DNA. Translation: AAF87846.1.
BC029896 mRNA. Translation: AAH29896.1.
CCDSiCCDS33088.1.
RefSeqiNP_003821.1. NM_003830.3.
UniGeneiHs.310333.

Genome annotation databases

EnsembliENST00000429354; ENSP00000415200; ENSG00000268500.
ENST00000534261; ENSP00000473238; ENSG00000105501.
ENST00000570106; ENSP00000455510; ENSG00000105501.
ENST00000599649; ENSP00000470259; ENSG00000268500.
GeneIDi8778.
KEGGihsa:8778.
UCSCiuc002pxe.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170484 mRNA. Translation: AAD50978.1.
U71383 mRNA. Translation: AAB70703.1.
AC018755 Genomic DNA. Translation: AAF87846.1.
BC029896 mRNA. Translation: AAH29896.1.
CCDSiCCDS33088.1.
RefSeqiNP_003821.1. NM_003830.3.
UniGeneiHs.310333.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZG1X-ray2.70A20-233[»]
2ZG2X-ray2.85A20-233[»]
2ZG3X-ray3.00A20-233[»]
ProteinModelPortaliO15389.
SMRiO15389. Positions 20-233, 259-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114308. 3 interactions.
IntActiO15389. 3 interactions.
MINTiMINT-1445881.
STRINGi9606.ENSP00000455510.

PTM databases

PhosphoSiteiO15389.

Polymorphism and mutation databases

BioMutaiSIGLEC5.

Proteomic databases

PRIDEiO15389.

Protocols and materials databases

DNASUi8778.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000429354; ENSP00000415200; ENSG00000268500.
ENST00000534261; ENSP00000473238; ENSG00000105501.
ENST00000570106; ENSP00000455510; ENSG00000105501.
ENST00000599649; ENSP00000470259; ENSG00000268500.
GeneIDi8778.
KEGGihsa:8778.
UCSCiuc002pxe.4. human.

Organism-specific databases

CTDi8778.
GeneCardsiGC19M052114.
HGNCiHGNC:10874. SIGLEC5.
HPAiCAB024900.
HPA009085.
MIMi604200. gene.
neXtProtiNX_O15389.
PharmGKBiPA35775.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
HOVERGENiHBG036161.
InParanoidiO15389.
KOiK06549.
OMAiPEKMDDE.
OrthoDBiEOG7JDQXC.
PhylomeDBiO15389.
TreeFamiTF332441.

Miscellaneous databases

EvolutionaryTraceiO15389.
GeneWikiiSIGLEC5.
GenomeRNAii8778.
NextBioi32916.
PROiO15389.
SOURCEiSearch...

Gene expression databases

BgeeiO15389.
CleanExiHS_SIGLEC5.
GenevisibleiO15389. HS.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33."
    Cornish A.L., Freeman S., Forbes G., Ni J., Zhang M., Cepeda M., Gentz R., Augustus M., Carter K.C., Crocker P.R.
    Blood 92:2123-2132(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT TRP-358.
    Tissue: Macrophage.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Erythroleukemia.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-499.
    Tissue: Brain.
  5. "Structural implications of Siglec-5-mediated sialoglycan recognition."
    Zhuravleva M.A., Trandem K., Sun P.D.
    J. Mol. Biol. 375:437-447(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 20-233 ALONE AND IN COMPLEX WITH ALPHA-LINKED SIALYLLACTOSES, DISULFIDE BONDS, SIALIC ACID BINDING SITES.

Entry informationi

Entry nameiSIGL5_HUMAN
AccessioniPrimary (citable) accession number: O15389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.