O15389 (SIGL5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialic acid-binding Ig-like lectin 5
Short name=Siglec-5

Alternative name(s):
CD33 antigen-like 2
Obesity-binding protein 2
Short name=OB-BP2
Short name=OB-binding protein 2
CD_antigen=CD170

Gene names

Name:	SIGLEC5
Synonyms:	CD33L2, OBBP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
Domain	Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Sequence similarities	Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Potential

Chain

17 – 551

535

Sialic acid-binding Ig-like lectin 5

PRO_0000014944

Regions

Topological domain

17 – 441

425

Extracellular Potential

Transmembrane

442 – 462

21

Helical; Potential

Topological domain

463 – 551

89

Cytoplasmic Potential

Domain

19 – 136

118

Ig-like V-type

Domain

146 – 229

84

Ig-like C2-type 1

Domain

236 – 330

95

Ig-like C2-type 2

Motif

518 – 523

6

ITIM motif

Motif

542 – 547

6

SLAM-like motif

Sites

Binding site

119

1

Sialic acid

Binding site

127

1

Sialic acid

Binding site

129

1

Sialic acid

Amino acid modifications

Glycosylation

100

1

N-linked (GlcNAc...) Potential

Glycosylation

210

1

N-linked (GlcNAc...) Potential

Glycosylation

231

1

N-linked (GlcNAc...) Potential

Glycosylation

253

1

N-linked (GlcNAc...) Potential

Glycosylation

328

1

N-linked (GlcNAc...) Potential

Glycosylation

375

1

N-linked (GlcNAc...) Potential

Glycosylation

384

1

N-linked (GlcNAc...) Potential

Glycosylation

393

1

N-linked (GlcNAc...) Potential

Disulfide bond

36 ↔ 170

Ref.6

Disulfide bond

41 ↔ 101

Ref.6

Disulfide bond

164 ↔ 213

Ref.6

Disulfide bond

269 ↔ 314

By similarity

Natural variations

Natural variant

72

1

V → A.
Corresponds to variant rs1973019 [ dbSNP | Ensembl ].

VAR_014249

Natural variant

215

1

M → V.
Corresponds to variant rs1807124 [ dbSNP | Ensembl ].

VAR_014250

Natural variant

322

1

F → S.
Corresponds to variant rs2278831 [ dbSNP | Ensembl ].

VAR_014251

Natural variant

358

1

R → W. Ref.1
Corresponds to variant rs8108074 [ dbSNP | Ensembl ].

VAR_049929

Natural variant

499

1

P → A. Ref.5
Corresponds to variant rs3829655 [ dbSNP | Ensembl ].

VAR_020087

Experimental info

Sequence conflict

309

1

E → K in AAD50978. Ref.1

Sequence conflict

388

1

A → P in AAD50978. Ref.1

Sequence conflict

403

1

S → N in AAD50978. Ref.1

Secondary structure

1

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551

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Beta strand

27 – 32

6

Beta strand

37 – 39

3

Beta strand

42 – 44

3

Beta strand

56 – 62

7

Helix

67 – 69

3

Beta strand

72 – 76

5

Beta strand

78 – 80

3

Turn

84 – 89

6

Beta strand

90 – 92

3

Helix

96 – 98

3

Beta strand

103 – 105

3

Helix

110 – 112

3

Beta strand

114 – 122

9

Turn

123 – 125

3

Beta strand

126 – 129

4

Beta strand

135 – 140

6

Beta strand

147 – 149

3

Beta strand

160 – 165

6

Beta strand

171 – 174

4

Beta strand

177 – 183

7

Beta strand

186 – 188

3

Turn

190 – 193

4

Beta strand

195 – 200

6

Turn

204 – 208

5

Beta strand

210 – 216

7

Beta strand

225 – 229

5

Sequences

	Sequence		Length	Mass (Da)
	O15389 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2FEA2B6B341EFEAF	FASTA	551	60,715
	10 20 30 40 50 60 MLPLLLLPLL WGGSLQEKPV YELQVQKSVT VQEGLCVLVP CSFSYPWRSW YSSPPLYVYW 70 80 90 100 110 120 FRDGEIPYYA EVVATNNPDR RVKPETQGRF RLLGDVQKKN CSLSIGDARM EDTGSYFFRV 130 140 150 160 170 180 ERGRDVKYSY QQNKLNLEVT ALIEKPDIHF LEPLESGRPT RLSCSLPGSC EAGPPLTFSW 190 200 210 220 230 240 TGNALSPLDP ETTRSSELTL TPRPEDHGTN LTCQMKRQGA QVTTERTVQL NVSYAPQTIT 250 260 270 280 290 300 IFRNGIALEI LQNTSYLPVL EGQALRLLCD APSNPPAHLS WFQGSPALNA TPISNTGILE 310 320 330 340 350 360 LRRVRSAEEG GFTCRAQHPL GFLQIFLNLS VYSLPQLLGP SCSWEAEGLH CRCSFRARPA 370 380 390 400 410 420 PSLCWRLEEK PLEGNSSQGS FKVNSSSAGP WANSSLILHG GLSSDLKVSC KAWNIYGSQS 430 440 450 460 470 480 GSVLLLQGRS NLGTGVVPAA LGGAGVMALL CICLCLIFFL IVKARRKQAA GRPEKMDDED 490 500 510 520 530 540 PIMGTITSGS RKKPWPDSPG DQASPPGDAP PLEEQKELHY ASLSFSEMKS REPKDQEAPS 550 TTEYSEIKTS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33." Cornish A.L., Freeman S., Forbes G., Ni J., Zhang M., Cepeda M., Gentz R., Augustus M., Carter K.C., Crocker P.R. Blood 92:2123-2132(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT TRP-358. Tissue: Macrophage.
[2]	"OB-BP1/Siglec-6. A leptin- and sialic acid-binding protein of the immunoglobulin superfamily." Patel N., Brinkman-Van der Linden E.C.M., Altmann S.W., Gish K.C., Balasubramanian S., Timans J.C., Peterson D., Bell M.P., Bazan J.F., Varki A., Kastelein R.A. J. Biol. Chem. 274:22729-22738(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Erythroleukemia.
[3]	Erratum Patel N., Brinkman-Van der Linden E.C.M., Altmann S.W., Gish K.C., Balasubramanian S., Timans J.C., Peterson D., Bell M.P., Bazan J.F., Varki A., Kastelein R.A. J. Biol. Chem. 274:28058-28058(1999)
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-499. Tissue: Brain.
[6]	"Structural implications of Siglec-5-mediated sialoglycan recognition." Zhuravleva M.A., Trandem K., Sun P.D. J. Mol. Biol. 375:437-447(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 20-233 ALONE AND IN COMPLEX WITH ALPHA-LINKED SIALYLLACTOSES, DISULFIDE BONDS, SIALIC ACID BINDING SITES.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Siglec-5

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF170484 mRNA. Translation: AAD50978.1.
U71383 mRNA. Translation: AAB70703.1.
AC018755 Genomic DNA. Translation: AAF87846.1.
BC029896 mRNA. Translation: AAH29896.1.

IPI

IPI00021935.

RefSeq

NP_003821.1. NM_003830.3.

UniGene

Hs.310333.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZG1	X-ray	2.70	A	20-233	[»]
2ZG2	X-ray	2.85	A	20-233	[»]
2ZG3	X-ray	3.00	A	20-233	[»]

ProteinModelPortal

O15389.

ModBase

Search...

Protein-protein interaction databases

IntAct

O15389. 2 interactions.

MINT

MINT-1445881.

STRING

9606.ENSP00000415200.

PTM databases

PhosphoSite

O15389.

Proteomic databases

PRIDE

O15389.

Protocols and materials databases

DNASU

8778.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000222107; ENSP00000222107; ENSG00000105501.
ENST00000534261; ENSP00000473238; ENSG00000105501.
ENST00000570106; ENSP00000455510; ENSG00000105501.

GeneID

8778.

KEGG

hsa:8778.

UCSC

uc002pxe.3. human.

Organism-specific databases

CTD

8778.

GeneCards

GC19M052114.

HGNC

HGNC:10874. SIGLEC5.

HPA

CAB024900.
HPA009085.

MIM

604200. gene.

neXtProt

NX_O15389.

PharmGKB

PA35775.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

HOG000236324.

HOVERGEN

HBG036161.

InParanoid

O15389.

KO

K06549.

OMA

PEKMDDE.

OrthoDB

EOG43BMP7.

PhylomeDB

O15389.

Gene expression databases

ArrayExpress

O15389.

Bgee

O15389.

CleanEx

HS_SIGLEC5.

Genevestigator

O15389.

GermOnline

ENSG00000105501. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 3 hits.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]

Pfam

PF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O15389.

GenomeRNAi

8778.

NextBio

32916.

SOURCE

Search...

Entry information

Entry name

SIGL5_HUMAN

Accession

Primary (citable) accession number: O15389

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.