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O15389

- SIGL5_HUMAN

UniProt

O15389 - SIGL5_HUMAN

Protein

Sialic acid-binding Ig-like lectin 5

Gene

SIGLEC5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei119 – 1191Sialic acid
    Binding sitei127 – 1271Sialic acid
    Binding sitei129 – 1291Sialic acid

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Lectin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialic acid-binding Ig-like lectin 5
    Short name:
    Siglec-5
    Alternative name(s):
    CD33 antigen-like 2
    Obesity-binding protein 2
    Short name:
    OB-BP2
    Short name:
    OB-binding protein 2
    CD_antigen: CD170
    Gene namesi
    Name:SIGLEC5
    Synonyms:CD33L2, OBBP2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:10874. SIGLEC5.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35775.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 551535Sialic acid-binding Ig-like lectin 5PRO_0000014944Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi36 ↔ 1701 PublicationPROSITE-ProRule annotation
    Disulfide bondi41 ↔ 1011 PublicationPROSITE-ProRule annotation
    Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi164 ↔ 2131 PublicationPROSITE-ProRule annotation
    Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi269 ↔ 314PROSITE-ProRule annotation
    Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi384 – 3841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO15389.

    PTM databases

    PhosphoSiteiO15389.

    Expressioni

    Tissue specificityi

    Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

    Gene expression databases

    BgeeiO15389.
    CleanExiHS_SIGLEC5.
    GenevestigatoriO15389.

    Organism-specific databases

    HPAiCAB024900.
    HPA009085.

    Interactioni

    Protein-protein interaction databases

    BioGridi114308. 2 interactions.
    IntActiO15389. 2 interactions.
    MINTiMINT-1445881.
    STRINGi9606.ENSP00000415200.

    Structurei

    Secondary structure

    1
    551
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi22 – 243
    Beta strandi27 – 326
    Beta strandi37 – 393
    Beta strandi42 – 443
    Beta strandi56 – 627
    Helixi67 – 693
    Beta strandi72 – 765
    Beta strandi78 – 803
    Turni84 – 896
    Beta strandi90 – 923
    Helixi96 – 983
    Beta strandi103 – 1053
    Helixi110 – 1123
    Beta strandi114 – 1229
    Turni123 – 1253
    Beta strandi126 – 1294
    Beta strandi135 – 1406
    Beta strandi147 – 1493
    Beta strandi160 – 1656
    Beta strandi171 – 1744
    Beta strandi177 – 1837
    Beta strandi186 – 1883
    Turni190 – 1934
    Beta strandi195 – 2006
    Turni204 – 2085
    Beta strandi210 – 2167
    Beta strandi225 – 2295

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZG1X-ray2.70A20-233[»]
    2ZG2X-ray2.85A20-233[»]
    2ZG3X-ray3.00A20-233[»]
    ProteinModelPortaliO15389.
    SMRiO15389. Positions 20-233.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO15389.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 441425ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini463 – 55189CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei442 – 46221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 136118Ig-like V-typeAdd
    BLAST
    Domaini146 – 22984Ig-like C2-type 1Add
    BLAST
    Domaini236 – 33095Ig-like C2-type 2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi518 – 5236ITIM motif
    Motifi542 – 5476SLAM-like motif

    Domaini

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000236324.
    HOVERGENiHBG036161.
    InParanoidiO15389.
    KOiK06549.
    OMAiPEKMDDE.
    OrthoDBiEOG7JDQXC.
    PhylomeDBiO15389.
    TreeFamiTF332441.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR013106. Ig_V-set.
    IPR013151. Immunoglobulin.
    [Graphical view]
    PfamiPF00047. ig. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    SM00408. IGc2. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 3 hits.
    PS00290. IG_MHC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O15389-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPLLLLPLL WGGSLQEKPV YELQVQKSVT VQEGLCVLVP CSFSYPWRSW    50
    YSSPPLYVYW FRDGEIPYYA EVVATNNPDR RVKPETQGRF RLLGDVQKKN 100
    CSLSIGDARM EDTGSYFFRV ERGRDVKYSY QQNKLNLEVT ALIEKPDIHF 150
    LEPLESGRPT RLSCSLPGSC EAGPPLTFSW TGNALSPLDP ETTRSSELTL 200
    TPRPEDHGTN LTCQMKRQGA QVTTERTVQL NVSYAPQTIT IFRNGIALEI 250
    LQNTSYLPVL EGQALRLLCD APSNPPAHLS WFQGSPALNA TPISNTGILE 300
    LRRVRSAEEG GFTCRAQHPL GFLQIFLNLS VYSLPQLLGP SCSWEAEGLH 350
    CRCSFRARPA PSLCWRLEEK PLEGNSSQGS FKVNSSSAGP WANSSLILHG 400
    GLSSDLKVSC KAWNIYGSQS GSVLLLQGRS NLGTGVVPAA LGGAGVMALL 450
    CICLCLIFFL IVKARRKQAA GRPEKMDDED PIMGTITSGS RKKPWPDSPG 500
    DQASPPGDAP PLEEQKELHY ASLSFSEMKS REPKDQEAPS TTEYSEIKTS 550
    K 551
    Length:551
    Mass (Da):60,715
    Last modified:January 1, 1998 - v1
    Checksum:i2FEA2B6B341EFEAF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti309 – 3091E → K in AAD50978. (PubMed:9731071)Curated
    Sequence conflicti388 – 3881A → P in AAD50978. (PubMed:9731071)Curated
    Sequence conflicti403 – 4031S → N in AAD50978. (PubMed:9731071)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti72 – 721V → A.
    Corresponds to variant rs1973019 [ dbSNP | Ensembl ].
    VAR_014249
    Natural varianti215 – 2151M → V.
    Corresponds to variant rs1807124 [ dbSNP | Ensembl ].
    VAR_014250
    Natural varianti322 – 3221F → S.
    Corresponds to variant rs2278831 [ dbSNP | Ensembl ].
    VAR_014251
    Natural varianti358 – 3581R → W.1 Publication
    Corresponds to variant rs8108074 [ dbSNP | Ensembl ].
    VAR_049929
    Natural varianti499 – 4991P → A.1 Publication
    Corresponds to variant rs3829655 [ dbSNP | Ensembl ].
    VAR_020087

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF170484 mRNA. Translation: AAD50978.1.
    U71383 mRNA. Translation: AAB70703.1.
    AC018755 Genomic DNA. Translation: AAF87846.1.
    BC029896 mRNA. Translation: AAH29896.1.
    CCDSiCCDS33088.1.
    RefSeqiNP_003821.1. NM_003830.3.
    UniGeneiHs.310333.

    Genome annotation databases

    EnsembliENST00000534261; ENSP00000473238; ENSG00000105501.
    ENST00000570106; ENSP00000455510; ENSG00000105501.
    GeneIDi8778.
    KEGGihsa:8778.
    UCSCiuc002pxe.4. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Siglec-5

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF170484 mRNA. Translation: AAD50978.1 .
    U71383 mRNA. Translation: AAB70703.1 .
    AC018755 Genomic DNA. Translation: AAF87846.1 .
    BC029896 mRNA. Translation: AAH29896.1 .
    CCDSi CCDS33088.1.
    RefSeqi NP_003821.1. NM_003830.3.
    UniGenei Hs.310333.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZG1 X-ray 2.70 A 20-233 [» ]
    2ZG2 X-ray 2.85 A 20-233 [» ]
    2ZG3 X-ray 3.00 A 20-233 [» ]
    ProteinModelPortali O15389.
    SMRi O15389. Positions 20-233.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114308. 2 interactions.
    IntActi O15389. 2 interactions.
    MINTi MINT-1445881.
    STRINGi 9606.ENSP00000415200.

    PTM databases

    PhosphoSitei O15389.

    Proteomic databases

    PRIDEi O15389.

    Protocols and materials databases

    DNASUi 8778.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000534261 ; ENSP00000473238 ; ENSG00000105501 .
    ENST00000570106 ; ENSP00000455510 ; ENSG00000105501 .
    GeneIDi 8778.
    KEGGi hsa:8778.
    UCSCi uc002pxe.4. human.

    Organism-specific databases

    CTDi 8778.
    GeneCardsi GC19M052114.
    HGNCi HGNC:10874. SIGLEC5.
    HPAi CAB024900.
    HPA009085.
    MIMi 604200. gene.
    neXtProti NX_O15389.
    PharmGKBi PA35775.
    GenAtlasi Search...

    Phylogenomic databases

    HOGENOMi HOG000236324.
    HOVERGENi HBG036161.
    InParanoidi O15389.
    KOi K06549.
    OMAi PEKMDDE.
    OrthoDBi EOG7JDQXC.
    PhylomeDBi O15389.
    TreeFami TF332441.

    Miscellaneous databases

    EvolutionaryTracei O15389.
    GeneWikii SIGLEC5.
    GenomeRNAii 8778.
    NextBioi 32916.
    PROi O15389.
    SOURCEi Search...

    Gene expression databases

    Bgeei O15389.
    CleanExi HS_SIGLEC5.
    Genevestigatori O15389.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR013106. Ig_V-set.
    IPR013151. Immunoglobulin.
    [Graphical view ]
    Pfami PF00047. ig. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    SM00408. IGc2. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 3 hits.
    PS00290. IG_MHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33."
      Cornish A.L., Freeman S., Forbes G., Ni J., Zhang M., Cepeda M., Gentz R., Augustus M., Carter K.C., Crocker P.R.
      Blood 92:2123-2132(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT TRP-358.
      Tissue: Macrophage.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Erythroleukemia.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-499.
      Tissue: Brain.
    5. "Structural implications of Siglec-5-mediated sialoglycan recognition."
      Zhuravleva M.A., Trandem K., Sun P.D.
      J. Mol. Biol. 375:437-447(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 20-233 ALONE AND IN COMPLEX WITH ALPHA-LINKED SIALYLLACTOSES, DISULFIDE BONDS, SIALIC ACID BINDING SITES.

    Entry informationi

    Entry nameiSIGL5_HUMAN
    AccessioniPrimary (citable) accession number: O15389
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3