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O15381 (NVL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear valosin-containing protein-like

Short name=NVLp
Short name=Nuclear VCP-like protein
Gene names
Name:NVL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with NCL/nucleolin. Ref.9

Subcellular location

Nucleusnucleolus Ref.1 Ref.5.

Tissue specificity

Widely expressed. Highest level of expression in heart, placenta, skeletal muscle, pancreas and retina. Ref.1

Sequence similarities

Belongs to the AAA ATPase family.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Traceable author statement Ref.1. Source: ProtInc

   Molecular_functionATP binding

Traceable author statement Ref.1. Source: ProtInc

nucleoside-triphosphatase activity

Inferred from electronic annotation. Source: InterPro

poly(A) RNA binding

Inferred from direct assay PubMed 22658674. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O15381-1)

Also known as: NVLp.2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O15381-2)

Also known as: NVLp.1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.
Isoform 3 (identifier: O15381-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.
     115-205: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: O15381-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.
     320-320: G → GAECSGMITAHCSFDFSGSNDPPASASQ
Isoform 5 (identifier: O15381-5)

The sequence of this isoform differs from the canonical sequence as follows:
     115-205: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 856856Nuclear valosin-containing protein-like
PRO_0000084588

Regions

Nucleotide binding305 – 3128ATP Potential
Nucleotide binding622 – 6298ATP Probable
Motif49 – 524Nucleolar localization signal By similarity

Amino acid modifications

Modified residue701N6-acetyllysine By similarity
Modified residue1341Phosphoserine Ref.6 Ref.7
Modified residue1381Phosphothreonine Ref.6 Ref.7
Modified residue1561N6-acetyllysine By similarity
Modified residue1911Phosphoserine By similarity
Modified residue2111Phosphoserine Ref.7 Ref.10
Modified residue2151Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 216216Missing in isoform 4.
VSP_045334
Alternative sequence1 – 106106Missing in isoform 2 and isoform 3.
VSP_007771
Alternative sequence115 – 20591Missing in isoform 3 and isoform 5.
VSP_007772
Alternative sequence3201G → GAECSGMITAHCSFDFSGSN DPPASASQ in isoform 4.
VSP_045335
Natural variant2951V → I.
Corresponds to variant rs12084919 [ dbSNP | Ensembl ].
VAR_048109
Natural variant3591C → G. Ref.1
VAR_015890
Natural variant4041V → I.
Corresponds to variant rs34631151 [ dbSNP | Ensembl ].
VAR_048110

Secondary structure

......................................... 856
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (NVLp.2) [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 7A8B373FCAFB99B7

FASTA85695,051
        10         20         30         40         50         60 
MKPRPAGFVD NKLKQRVIQY LTSNKCGKYV DIGVLASDLQ RVYSIDYGRR KRNAFRIQVE 

        70         80         90        100        110        120 
KVFSIISSEK ELKNLTELED EHLAKRARQG EEDNEYTESY SDDDSSMEDY PDPQSANHMN 

       130        140        150        160        170        180 
SSLLSLYRKG NPDSVSNTPE MEQRETTSST PRISSKTGSI PLKTPAKDSE GGWFIDKTPS 

       190        200        210        220        230        240 
VKKDSFFLDL SCEKSNPKKP ITEIQDSKDS SLLESDMKRK GKLKNKGSKR KKEDLQEVDG 

       250        260        270        280        290        300 
EIEAVLQKKA KARGLEFQIS NVKFEDVGGN DMTLKEVCKM LIHMRHPEVY HHLGVVPPRG 

       310        320        330        340        350        360 
VLLHGPPGCG KTLLAHAIAG ELDLPILKVA APEIVSGVSG ESEQKLRELF EQAVSNAPCI 

       370        380        390        400        410        420 
IFIDEIDAIT PKREVASKDM ERRIVAQLLT CMDDLNNVAA TARVLVIGAT NRPDSLDPAL 

       430        440        450        460        470        480 
RRAGRFDREI CLGIPDEASR ERILQTLCRK LRLPQAFDFC HLAHLTPGFV GADLMALCRE 

       490        500        510        520        530        540 
AAMCAVNRVL MKLQEQQKKN PEMEDLPSKG VQEERLGTEP TSETQDELQR LLGLLRDQDP 

       550        560        570        580        590        600 
LSEEQMQGLC IELNDFIVAL SSVQPSAKRE GFVTVPNVTW ADIGALEDIR EELTMAILAP 

       610        620        630        640        650        660 
VRNPDQFKAL GLVTPAGVLL AGPPGCGKTL LAKAVANESG LNFISVKGPE LLNMYVGESE 

       670        680        690        700        710        720 
RAVRQVFQRA KNSAPCVIFF DEVDALCPRR SDRETGASVR VVNQLLTEMD GLEARQQVFI 

       730        740        750        760        770        780 
MAATNRPDII DPAILRPGRL DKTLFVGLPP PADRLAILKT ITKNGTKPPL DADVNLEAIA 

       790        800        810        820        830        840 
GDLRCDCYTG ADLSALVREA SICALRQEMA RQKSGNEKGE LKVSHKHFEE AFKKVRSSIS 

       850 
KKDQIMYERL QESLSR 

« Hide

Isoform 2 (NVLp.1) [UniParc].

Checksum: 56AF88DF9129D618
Show »

FASTA75082,746
Isoform 3 [UniParc].

Checksum: 0728D502901260F5
Show »

FASTA65972,733
Isoform 4 [UniParc].

Checksum: E46F119F9E1C345A
Show »

FASTA66773,280
Isoform 5 [UniParc].

Checksum: A34DEE65ED8A82D6
Show »

FASTA76585,038

References

« Hide 'large scale' references
[1]"NVL: a new member of the AAA family of ATPases localized to the nucleus."
Germain-Lee E.L., Obie C., Valle D.
Genomics 44:22-34(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT GLY-359, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Kidney.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4 AND 5).
Tissue: Brain.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Eye.
[5]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134 AND THR-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134; THR-138; SER-211 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Structure and function of the N-terminal nucleolin binding domain of nuclear valosin-containing protein-like 2 (NVL2) harboring a nucleolar localization signal."
Fujiwara Y., Fujiwara K., Goda N., Iwaya N., Tenno T., Shirakawa M., Hiroaki H.
J. Biol. Chem. 286:21732-21741(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NCL/NUCLEOLIN.
[10]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-211, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Human nuclear valosin containing protein like (NVL), C-terminal AAA-ATPase domain."
Structural genomics consortium (SGC)
Submitted (MAR-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 574-845 IN COMPLEX WITH PHOSPHATE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U68140 mRNA. Translation: AAB70457.1.
U78772 mRNA. Translation: AAB70460.1.
AK297396 mRNA. Translation: BAG59836.1.
AK298244 mRNA. Translation: BAG60510.1.
AC092809 Genomic DNA. No translation available.
BC012105 mRNA. Translation: AAH12105.1.
CCDSCCDS1541.1. [O15381-1]
CCDS1542.1. [O15381-2]
CCDS58062.1. [O15381-4]
CCDS58063.1. [O15381-5]
RefSeqNP_001230075.1. NM_001243146.1. [O15381-4]
NP_001230076.1. NM_001243147.1. [O15381-5]
NP_002524.2. NM_002533.3. [O15381-1]
NP_996671.1. NM_206840.2. [O15381-2]
UniGeneHs.497867.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2X8AX-ray2.60A574-845[»]
ProteinModelPortalO15381.
SMRO15381. Positions 1-74, 256-854.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110985. 7 interactions.
IntActO15381. 4 interactions.
MINTMINT-1423251.
STRING9606.ENSP00000281701.

PTM databases

PhosphoSiteO15381.

2D gel databases

SWISS-2DPAGEO15381.

Proteomic databases

MaxQBO15381.
PaxDbO15381.
PRIDEO15381.

Protocols and materials databases

DNASU4931.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000281701; ENSP00000281701; ENSG00000143748. [O15381-1]
ENST00000340871; ENSP00000341362; ENSG00000143748. [O15381-4]
ENST00000391875; ENSP00000375747; ENSG00000143748. [O15381-2]
ENST00000469075; ENSP00000417826; ENSG00000143748. [O15381-5]
GeneID4931.
KEGGhsa:4931.
UCSCuc001hok.3. human. [O15381-1]
uc010pvd.2. human.
uc010pve.2. human.

Organism-specific databases

CTD4931.
GeneCardsGC01M224416.
HGNCHGNC:8070. NVL.
HPAHPA028207.
MIM602426. gene.
neXtProtNX_O15381.
PharmGKBPA31857.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0464.
HOGENOMHOG000223225.
HOVERGENHBG001226.
InParanoidO15381.
KOK14571.
OMAELHMCIV.
PhylomeDBO15381.
TreeFamTF314681.

Gene expression databases

ArrayExpressO15381.
BgeeO15381.
CleanExHS_NVL.
GenevestigatorO15381.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00674. AAA. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNVL. human.
EvolutionaryTraceO15381.
GeneWikiNVL_(gene).
GenomeRNAi4931.
NextBio19001.
PROO15381.
SOURCESearch...

Entry information

Entry nameNVL_HUMAN
AccessionPrimary (citable) accession number: O15381
Secondary accession number(s): B4DMC4, B4DP98, Q96EM7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM