Reviewed, UniProtKB/Swiss-Prot O15355 (PPM1G_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 1G
    EC=3.1.3.16
Alternative name(s):
    Protein phosphatase 2C isoform gamma
      Short name=PP2C-gamma
    Protein phosphatase magnesium-dependent 1 gamma
    Protein phosphatase 1C

Gene names

Name:	PPM1G
Synonyms:	PPM1C

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Widely expressed. Most abundant in testis, skeletal muscle, and heart.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.3 Ref.4
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle arrest Traceable author statement. Source: ProtInc protein amino acid dephosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Traceable author statement. Source: ProtInc protein serine/threonine phosphatase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Protein phosphatase 1G		PRO_0000057750
Regions
	Compositional bias	258 – 326	69	Asp/Glu-rich (acidic)
	Compositional bias	266 – 272	7	Poly-Glu
	Compositional bias	306 – 311	6	Poly-Glu
	Compositional bias	539 – 544	6	Poly-Lys
Sites
	Metal binding	60	1	Manganese 1 By similarity
	Metal binding	60	1	Manganese 2 By similarity
	Metal binding	61	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	441	1	Manganese 2 By similarity
	Metal binding	496	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	183	1	Phosphoserine Ref.4
	Modified residue	527	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O15355-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 084C16F8252330D9	FASTA	546	59,272
	10 20 30 40 50 60 MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD 70 80 90 100 110 120 GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR 130 140 150 160 170 180 PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE 190 200 210 220 230 240 PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS 250 260 270 280 290 300 CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE 310 320 330 340 350 360 AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL 370 380 390 400 410 420 DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA 430 440 450 460 470 480 LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL 490 500 510 520 530 540 DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PP2C gamma: a human protein phosphatase with a unique acidic domain." Travis S.M., Welsh M.J. FEBS Lett. 412:415-419(1997) [PubMed: 9276438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Skin.
[3]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, MASS SPECTROMETRY.
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183, MASS SPECTROMETRY.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y13936 mRNA. Translation: CAA74245.1. BC022061 mRNA. Translation: AAH22061.1. BC000057 mRNA. Translation: AAH00057.1.
IPI	IPI00006167.
RefSeq	NP_002698.1. NP_817092.1.
UniGene	Hs.643951
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15355. 3 interactions.
PTM databases
PhosphoSite	O15355.
Proteomic databases
PeptideAtlas	O15355.
PRIDE	O15355.
Genome annotation databases
Ensembl	ENSG00000115241. Homo sapiens. [Contig view]
GeneID	5496.
KEGG	hsa:5496.
Organism-specific databases
GeneCards	GC02M027457.
H-InvDB	HIX0001922.
HGNC	HGNC:9278. PPM1G.
MIM	605119. gene.
PharmGKB	PA33606.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O15355.
HOVERGEN	O15355.
OMA	O15355. HSKSGAG.
Enzyme and pathway databases
BRENDA	3.1.3.16. 247.
Gene expression databases
ArrayExpress	O15355.
Bgee	O15355.
CleanEx	HS_PPM1G.
GermOnline	ENSG00000115241. Homo sapiens.
Family and domain databases
InterPro	IPR015655. PP2C. IPR001932. PP2C-related. IPR000222. PP2C_Mn2_Asp60_BS. IPR014045. PP2C_N. [Graphical view]
Gene3D	G3DSA:3.60.40.10. PP2C-related. 1 hit.
PANTHER	PTHR13832. PP2C. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	21262.
SOURCE	Search...

Entry information

Entry name

PPM1G_HUMAN

Accession

Primary (citable) accession number: O15355

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)