O15355 (PPM1G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1G
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 1C
Protein phosphatase 2C isoform gamma
Short name=PP2C-gamma
Protein phosphatase magnesium-dependent 1 gamma

Gene names

Name:	PPM1G
Synonyms:	PPM1C

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Widely expressed. Most abundant in testis, skeletal muscle, and heart.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest Traceable author statement PubMed 9271424. Source: ProtInc peptidyl-threonine dephosphorylation Inferred from direct assay PubMed 20801214. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 20801214. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tat	P04608	3	EBI-725702,EBI-6164389	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Protein phosphatase 1G		PRO_0000057750
Regions
	Compositional bias	258 – 326	69	Asp/Glu-rich (acidic)
	Compositional bias	266 – 272	7	Poly-Glu
	Compositional bias	306 – 311	6	Poly-Glu
	Compositional bias	539 – 544	6	Poly-Lys
Sites
	Metal binding	60	1	Manganese 1 By similarity
	Metal binding	60	1	Manganese 2 By similarity
	Metal binding	61	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	441	1	Manganese 2 By similarity
	Metal binding	496	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	183	1	Phosphoserine Ref.3
	Modified residue	383	1	N6-acetyllysine Ref.6
	Modified residue	527	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	O15355 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 084C16F8252330D9	FASTA	546	59,272
	10 20 30 40 50 60 MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD 70 80 90 100 110 120 GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR 130 140 150 160 170 180 PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE 190 200 210 220 230 240 PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS 250 260 270 280 290 300 CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE 310 320 330 340 350 360 AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL 370 380 390 400 410 420 DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA 430 440 450 460 470 480 LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL 490 500 510 520 530 540 DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PP2C gamma: a human protein phosphatase with a unique acidic domain." Travis S.M., Welsh M.J. FEBS Lett. 412:415-419(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Skin.
[3]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-383, MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13936 mRNA. Translation: CAA74245.1. BC022061 mRNA. Translation: AAH22061.1. BC000057 mRNA. Translation: AAH00057.1.
IPI	IPI00006167.
RefSeq	NP_817092.1. NM_177983.2.
UniGene	Hs.643951.
3D structure databases
ProteinModelPortal	O15355.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29404N.
IntAct	O15355. 12 interactions.
MINT	MINT-5003792.
STRING	9606.ENSP00000264714.
PTM databases
PhosphoSite	O15355.
Proteomic databases
PaxDb	O15355.
PeptideAtlas	O15355.
PRIDE	O15355.
Protocols and materials databases
DNASU	5496.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000344034; ENSP00000342778; ENSG00000115241. ENST00000350803; ENSP00000264714; ENSG00000115241.
GeneID	5496.
KEGG	hsa:5496.
UCSC	uc002rkl.3. human.
Organism-specific databases
CTD	5496.
GeneCards	GC02M027604.
HGNC	HGNC:9278. PPM1G.
HPA	HPA035530. HPA035531.
MIM	605119. gene.
neXtProt	NX_O15355.
PharmGKB	PA33606.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
HOVERGEN	HBG053647.
InParanoid	O15355.
KO	K01090.
OMA	MISAMPD.
OrthoDB	EOG4TQM8V.
PhylomeDB	O15355.
Gene expression databases
ArrayExpress	O15355.
Bgee	O15355.
CleanEx	HS_PPM1G.
Genevestigator	O15355.
GermOnline	ENSG00000115241. Homo sapiens.
Family and domain databases
Gene3D	3.60.40.10. 2 hits.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 2 hits. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PPM1G. human.
GenomeRNAi	5496.
NextBio	21262.
SOURCE	Search...

Entry information

Entry name

PPM1G_HUMAN

Accession

Primary (citable) accession number: O15355

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.