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O15335 (CHAD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chondroadherin
Alternative name(s):
Cartilage leucine-rich protein
Gene names
Name:CHAD
Synonyms:SLRR4A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Promotes attachment of chondrocytes, fibroblasts, and osteoblasts. This binding is mediated (at least for chondrocytes and fibroblasts) by the integrin alpha2beta1. May play an important role in the regulation of chondrocyte growth and proliferation By similarity.

Subunit structure

Mostly monomeric By similarity. Interacts with collagen type II.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Present in chondrocytes at all ages.

Sequence similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class IV subfamily.

Contains 9 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 LRRNT domain.

Ontologies

Keywords
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityPolymorphism
   DomainLeucine-rich repeat
Repeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcartilage condensation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentproteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 359337Chondroadherin
PRO_0000032773

Regions

Domain23 – 5230LRRNT
Repeat76 – 9722LRR 1
Repeat100 – 12122LRR 2
Repeat124 – 14522LRR 3
Repeat148 – 16922LRR 4
Repeat172 – 19322LRR 5
Repeat196 – 21722LRR 6
Repeat220 – 24122LRR 7
Repeat245 – 26622LRR 8
Repeat269 – 29022LRR 9
Domain300 – 34849LRRCT

Amino acid modifications

Glycosylation1441O-linked (GalNAc...) Potential
Disulfide bond23 ↔ 38 By similarity
Disulfide bond304 ↔ 346 By similarity
Disulfide bond306 ↔ 326 By similarity

Natural variations

Natural variant3121R → Q.
Corresponds to variant rs35218093 [ dbSNP | Ensembl ].
VAR_052019
Natural variant3501T → I.
Corresponds to variant rs2231510 [ dbSNP | Ensembl ].
VAR_030631

Experimental info

Sequence conflict1141L → V in AAC13410. Ref.1
Sequence conflict1661A → P in AAC13410. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O15335 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: CF24D2B5A5DFCF0C

FASTA35940,476
        10         20         30         40         50         60 
MVRPMLLLSL GLLAGLLPAL AACPQNCHCH SDLQHVICDK VGLQKIPKVS EKTKLLNLQR 

        70         80         90        100        110        120 
NNFPVLAANS FRAMPNLVSL HLQHCQIREV AAGAFRGLKQ LIYLYLSHND IRVLRAGAFD 

       130        140        150        160        170        180 
DLTELTYLYL DHNKVTELPR GLLSPLVNLF ILQLNNNKIR ELRAGAFQGA KDLRWLYLSE 

       190        200        210        220        230        240 
NALSSLQPGA LDDVENLAKF HVDRNQLSSY PSAALSKLRV VEELKLSHNP LKSIPDNAFQ 

       250        260        270        280        290        300 
SFGRYLETLW LDNTNLEKFS DGAFLGVTTL KHVHLENNRL NQLPSNFPFD SLETLALTNN 

       310        320        330        340        350 
PWKCTCQLRG LRRWLEAKAS RPDATCASPA KFKGQHIRDT DAFRSCKFPT KRSKKAGRH 

« Hide

References

« Hide 'large scale' references
[1]"The structure and chromosome location of the human chondroadherin gene (CHAD)."
Grover J., Chen X.-N., Korenberg J.R., Roughley P.J.
Genomics 45:379-385(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Association of chondroadherin with collagen type II."
Maansson B., Wenglen C., Moergelin M., Saxne T., Heinegaard D.
J. Biol. Chem. 276:32883-32888(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U96769, U96767, U96768 Genomic DNA. Translation: AAC13410.1.
AF371328 mRNA. Translation: AAK51556.1.
AK292177 mRNA. Translation: BAF84866.1.
CH471109 Genomic DNA. Translation: EAW94613.1.
BC036360 mRNA. Translation: AAH36360.1.
BC073974 mRNA. Translation: AAH73974.1.
CCDSCCDS11568.1.
RefSeqNP_001258.2. NM_001267.2.
UniGeneHs.97220.

3D structure databases

ProteinModelPortalO15335.
SMRO15335. Positions 23-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000258969.

Proteomic databases

PaxDbO15335.
PRIDEO15335.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000258969; ENSP00000258969; ENSG00000136457.
ENST00000508540; ENSP00000423812; ENSG00000136457.
GeneID1101.
KEGGhsa:1101.
UCSCuc010dbr.3. human.

Organism-specific databases

CTD1101.
GeneCardsGC17M048541.
HGNCHGNC:1909. CHAD.
HPAHPA018241.
MIM602178. gene.
neXtProtNX_O15335.
PharmGKBPA26445.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4886.
HOGENOMHOG000013150.
HOVERGENHBG005324.
InParanoidO15335.
KOK06248.
OMACHCHGGD.
PhylomeDBO15335.
TreeFamTF332659.

Gene expression databases

BgeeO15335.
CleanExHS_CHAD.
GenevestigatorO15335.

Family and domain databases

InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
[Graphical view]
PfamPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 2 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 5 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEPS51450. LRR. 10 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi1101.
NextBio4554.
PROO15335.
SOURCESearch...

Entry information

Entry nameCHAD_HUMAN
AccessionPrimary (citable) accession number: O15335
Secondary accession number(s): A8K812, Q6GTU0, Q96RJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM