O15318 (RPC7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase III subunit RPC7
Short name=RNA polymerase III subunit C7

Alternative name(s):
DNA-directed RNA polymerase III subunit G
RNA polymerase III 32 kDa subunit
Short name=RPC32

Gene names

Name:

POLR3G

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Ref.8 Ref.9
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the eukaryotic RPC7 RNA polymerase subunit family.
Sequence caution	The sequence AAB63676.1 differs from that shown. Reason: Frameshift at position 214.

Ontologies

Keywords
Biological process	Antiviral defense Immunity Innate immunity Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	innate immune response Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of innate immune response Inferred from mutant phenotype Ref.9Ref.8. Source: UniProtKB positive regulation of interferon-beta production Inferred from mutant phenotype Ref.9Ref.8. Source: UniProtKB regulation of transcription from RNA polymerase III promoter Traceable author statement. Source: ProtInc response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA-directed RNA polymerase III complex Traceable author statement. Source: ProtInc
Molecular function	DNA-directed RNA polymerase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	DNA-directed RNA polymerase III subunit RPC7		PRO_0000073978
Regions
	Compositional bias	147 – 196	50	Glu-rich
	Compositional bias	175 – 181	7	Poly-Asp
Amino acid modifications
	Modified residue	133	1	Phosphothreonine Ref.7
	Modified residue	157	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	96	1	K → Q in AAB63676. Ref.1
	Sequence conflict	146	1	E → V in AAB63676. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O15318 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 95E506D527962DD9	FASTA	223	25,914
	10 20 30 40 50 60 MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT GEGEEYMLAL 70 80 90 100 110 120 KQELRETMKR MPYFIETPEE RQDIERYSKR YMKVYKEEWI PDWRRLPREM MPRNKCKKAG 130 140 150 160 170 180 PKPKKAKDAG KGTPLTNTED VLKKMEELEK RGDGEKSDEE NEEKEGSKEK SKEGDDDDDD 190 200 210 220 DAAEQEEYDE EEQEEENDYI NSYFEDGDDF GADSDDNMDE ATY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation." Wang Z., Roeder R.G. Genes Dev. 11:1315-1326(1997) [PubMed: 9171375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH POLR3C AND POLR3F. Tissue: Cervix carcinoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Corpus callosum.
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits." Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N. Mol. Cell. Biol. 22:8044-8055(2002) [PubMed: 12391170] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY.
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-133, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway." Chiu Y.-H., Macmillan J.B., Chen Z.J. Cell 138:576-591(2009) [PubMed: 19631370] [Abstract] Cited for: FUNCTION.
[9]	"RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate." Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V. Nat. Immunol. 10:1065-1072(2009) [PubMed: 19609254] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U93868 mRNA. Translation: AAB63676.1. Frameshift. AK295434 mRNA. Translation: BAG58376.1. AC027323 Genomic DNA. No translation available. AC093510 Genomic DNA. No translation available. CH471084 Genomic DNA. Translation: EAW95980.1.
IPI	IPI00968202.
RefSeq	NP_006458.2. NM_006467.2.
UniGene	Hs.282387.
3D structure databases
ProteinModelPortal	O15318.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59079N.
STRING	O15318.
PTM databases
PhosphoSite	O15318.
Proteomic databases
PRIDE	O15318.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000399107; ENSP00000382058; ENSG00000113356.
GeneID	10622.
KEGG	hsa:10622.
Organism-specific databases
CTD	10622.
GeneCards	GC05P089807.
H-InvDB	HIX0005024.
HGNC	HGNC:30075. POLR3G.
HPA	CAB010109.
neXtProt	NX_O15318.
PharmGKB	PA134986024.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17058.
GeneTree	ENSGT00390000002422.
HOGENOM	HBG446471.
HOVERGEN	HBG054922.
InParanoid	O15318.
OMA	VPDWRRL.
Gene expression databases
ArrayExpress	O15318.
Bgee	O15318.
CleanEx	HS_POLR3G.
Genevestigator	O15318.
GermOnline	ENSG00000113356. Homo sapiens.
Family and domain databases
InterPro	IPR024661. RNA_pol_III_Rpc31. [Graphical view]
KO	K03024.
Pfam	PF11705. RNA_pol_3_Rpc31. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC7_HUMAN

Accession

Primary (citable) accession number: O15318
Secondary accession number(s): A8MTH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.