O15263 (DFB4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-defensin 4A

Alternative name(s):
Beta-defensin 2
Short name=BD-2
Short name=hBD-2
Defensin, beta 2
Skin-antimicrobial peptide 1
Short name=SAP1

Gene names

Name:	DEFB4A
Synonyms:	DEFB102, DEFB2, DEFB4
AND
Name:	DEFB4B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	64 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has antibacterial activity Potential.
Subcellular location	Secreted.
Tissue specificity	Expressed in the skin and respiratory tract.
Induction	By inflammation.
Sequence similarities	Belongs to the beta-defensin family. LAP/TAP subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Defensin
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 10521347. Source: ProtInc chemotaxis Traceable author statement PubMed 10521347. Source: ProtInc defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Traceable author statement. Source: Reactome
Cellular_component	Golgi lumen Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Potential

Peptide

24 – 64

41

Beta-defensin 4A

PRO_0000006968

Amino acid modifications

Disulfide bond

31 ↔ 60

Disulfide bond

38 ↔ 53

Disulfide bond

43 ↔ 61

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

64

Helix Strand Turn

Details...

Beta strand

25 – 27

3

Helix

28 – 33

6

Beta strand

37 – 41

5

Beta strand

48 – 52

5

Beta strand

53 – 55

3

Beta strand

59 – 62

4

Sequences

	Sequence		Length	Mass (Da)
	O15263 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 05D6454CE7ACD10E	FASTA	64	7,038
	10 20 30 40 50 60 MRVLYLLFSF LFIFLMPLPG VFGGIGDPVT CLKSGAICHP VFCPRRYKQI GTCGLPGTKC CKKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A peptide antibiotic from human skin." Harder J., Bartels J.H., Christophers E., Schroeder J.-M. Nature 387:861-861(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin.
[2]	"Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation." Liu L., Wang L., Jia H.P., Zhao C., Heng H.H.Q., Schutte B.C., McCray P.B. Jr., Ganz T. Gene 222:237-244(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[3]	"Transcriptional regulation of beta-defensin gene expression in tracheal epithelial cells." Diamond G., Kaiser V., Rhodes J., Russell J.P., Bevins C.L. Infect. Immun. 68:113-119(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia." Harder J., Meyer-Hoffert U., Teran L.M., Schwichtenberg L., Bartels J., Maune S., Schroeder J.-M. Am. J. Respir. Cell Mol. Biol. 22:714-721(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Chemical synthesis of beta-defensins and LEAP-1/hepcidin." Kluever E., Schulz A., Forssmann W.-G., Adermann K. J. Pept. Res. 59:241-248(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SYNTHESIS OF 24-64.
[7]	"The structure of human beta-defensin-2 shows evidence of higher order oligomerization." Hoover D.M., Rajashankar K.R., Blumenthal R., Puri A., Oppenheim J.J., Chertov O., Lubkowski J. J. Biol. Chem. 275:32911-32918(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS).
[8]	"Structure determination of human and murine beta-defensins reveals structural conservation in the absence of significant sequence similarity." Bauer F., Schweimer K., Kluever E., Conejo-Garcia J.-R., Forssmann W.-G., Roesch P., Adermann K., Sticht H. Protein Sci. 10:2470-2479(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 28-64.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z71389 mRNA. Translation: CAA95992.1.
AF040153 Genomic DNA. Translation: AAC33549.1.
AF071216 Genomic DNA. Translation: AAC69554.1.
AJ000152 Genomic DNA. Translation: CAB65126.1.
BC069285 mRNA. Translation: AAH69285.1.
BC093983 mRNA. Translation: AAH93983.1.
BC093985 mRNA. Translation: AAH93985.1.

IPI

IPI00005739.

RefSeq

NP_001192195.1. NM_001205266.1.
NP_004933.1. NM_004942.2.

UniGene

Hs.105924.
Hs.740237.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E4Q	NMR	-	A	28-64	[»]
1FD3	X-ray	1.35	A/B/C/D	24-64	[»]
1FD4	X-ray	1.70	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P	24-64	[»]
1FQQ	NMR	-	A	24-64	[»]

ProteinModelPortal

O15263.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000303532.

Protein family/group databases

TCDB

1.C.85.1.2. pore-forming beta,-defensin (beta,-Defensin) family.

Proteomic databases

PRIDE

O15263.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000302247; ENSP00000303532; ENSG00000171711.
ENST00000318157; ENSP00000424598; ENSG00000177257.

GeneID

100289462.
1673.

KEGG

hsa:100289462.
hsa:1673.

UCSC

uc003wsd.3. human.

Organism-specific databases

CTD

100289462.
1673.

GeneCards

GC08M007272.
GC08P007752.

HGNC

HGNC:2767. DEFB4A.
HGNC:30193. DEFB4B.

MIM

602215. gene.

neXtProt

NX_O15263.

PharmGKB

PA27249.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG84369.

HOGENOM

HOG000015465.

HOVERGEN

HBG004834.

InParanoid

O15263.

OMA

SGAICHP.

OrthoDB

EOG4WM4WG.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

CleanEx

HS_DEFB4.

Genevestigator

O15263.

GermOnline

ENSG00000171711. Homo sapiens.
ENSG00000177257. Homo sapiens.

Family and domain databases

InterPro

IPR001855. Defensin_beta-typ.
IPR006080. Defensin_beta/neutrophil.
[Graphical view]

Pfam

PF00711. Defensin_beta. 1 hit.
[Graphical view]

SMART

SM00048. DEFSN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O15263.

NextBio

20779203.

SOURCE

Search...

Entry information

Entry name

DFB4A_HUMAN

Accession

Primary (citable) accession number: O15263
Secondary accession number(s): Q52LC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.