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Protein

Surfeit locus protein 4

Gene

SURF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the maintenance of the architecture of the endoplasmic reticulum-Golgi intermediate compartment and of the Golgi.1 Publication

GO - Biological processi

  • Golgi organization Source: UniProtKB
  • positive regulation of organelle organization Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Surfeit locus protein 4
Gene namesi
Name:SURF4
Synonyms:SURF-4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:11476. SURF4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei64 – 8421HelicalSequence analysisAdd
BLAST
Transmembranei92 – 11221HelicalSequence analysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence analysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence analysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi265 – 2673KKK → SSS: Targeted to the Golgi. 1 Publication

Organism-specific databases

PharmGKBiPA36261.

Polymorphism and mutation databases

BioMutaiSURF4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 269268Surfeit locus protein 4PRO_0000127664Add
BLAST

Proteomic databases

EPDiO15260.
MaxQBiO15260.
PaxDbiO15260.
PRIDEiO15260.
TopDownProteomicsiO15260-1. [O15260-1]

PTM databases

iPTMnetiO15260.
PhosphoSiteiO15260.
SwissPalmiO15260.

Expressioni

Gene expression databases

BgeeiO15260.
CleanExiHS_SURF4.
ExpressionAtlasiO15260. baseline and differential.
GenevisibleiO15260. HS.

Interactioni

Subunit structurei

Found in a complex composed at least of SURF4, TMED2 and TMED10. May interact with LMAN1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LMAN1P492573EBI-1044848,EBI-1057738
TMEM173Q86WV62EBI-1044848,EBI-2800345
Unc93b1Q8VCW42EBI-1044848,EBI-6116986From a different organism.

Protein-protein interaction databases

BioGridi112703. 53 interactions.
IntActiO15260. 23 interactions.
MINTiMINT-2998714.
STRINGi9606.ENSP00000361057.

Structurei

3D structure databases

ProteinModelPortaliO15260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi266 – 2694Di-lysine motif

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.

Sequence similaritiesi

Belongs to the SURF4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3998. Eukaryota.
COG2259. LUCA.
GeneTreeiENSGT00530000064123.
HOGENOMiHOG000206959.
HOVERGENiHBG054238.
InParanoidiO15260.
OMAiDQLWYLQ.
OrthoDBiEOG7GTT4K.
PhylomeDBiO15260.
TreeFamiTF300001.

Family and domain databases

InterProiIPR002995. Surf4.
[Graphical view]
PfamiPF02077. SURF4. 1 hit.
[Graphical view]
PROSITEiPS01339. SURF4. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15260-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQNDLMGTA EDFADQFLRV TKQYLPHVAR LCLISTFLED GIRMWFQWSE
60 70 80 90 100
QRDYIDTTWN CGYLLASSFV FLNLLGQLTG CVLVLSRNFV QYACFGLFGI
110 120 130 140 150
IALQTIAYSI LWDLKFLMRN LALGGGLLLL LAESRSEGKS MFAGVPTMRE
160 170 180 190 200
SSPKQYMQLG GRVLLVLMFM TLLHFDASFF SIVQNIVGTA LMILVAIGFK
210 220 230 240 250
TKLAALTLVV WLFAINVYFN AFWTIPVYKP MHDFLKYDFF QTMSVIGGLL
260
LVVALGPGGV SMDEKKKEW
Length:269
Mass (Da):30,394
Last modified:December 1, 2000 - v3
Checksum:i03366E29882B97A5
GO
Isoform 2 (identifier: O15260-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-159: MQL → LPG
     160-269: Missing.

Show »
Length:159
Mass (Da):17,970
Checksum:i0073439E58A7E274
GO
Isoform 3 (identifier: O15260-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     120-128: NLALGGGLL → LSRTSWAQL
     129-269: Missing.

Note: No experimental confirmation available.
Show »
Length:128
Mass (Da):14,854
Checksum:i109C00EB530DECD4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1301L → V in CAA75099 (PubMed:7540914).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei120 – 1289NLALGGGLL → LSRTSWAQL in isoform 3. 1 PublicationVSP_054686
Alternative sequencei129 – 269141Missing in isoform 3. 1 PublicationVSP_054687Add
BLAST
Alternative sequencei157 – 1593MQL → LPG in isoform 2. 1 PublicationVSP_006307
Alternative sequencei160 – 269110Missing in isoform 2. 1 PublicationVSP_006308Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078866 mRNA. Translation: AAD44498.1.
AF078867 mRNA. Translation: AAD44499.1.
AK300004 mRNA. Translation: BAH13190.1.
AK315488 mRNA. Translation: BAG37872.1.
AL158826 Genomic DNA. Translation: CAI12840.1.
CH471090 Genomic DNA. Translation: EAW88071.1.
CH471090 Genomic DNA. Translation: EAW88074.1.
BC018741 mRNA. Translation: AAH18741.1.
BC111023 mRNA. Translation: AAI11024.1.
Y14820 mRNA. Translation: CAA75099.1.
Y17214 Genomic DNA. Translation: CAA76692.1.
CCDSiCCDS65177.1. [O15260-3]
CCDS65178.1. [O15260-2]
CCDS6968.1. [O15260-1]
RefSeqiNP_001267718.1. NM_001280789.1. [O15260-2]
NP_001267721.1. NM_001280792.1. [O15260-3]
NP_149351.1. NM_033161.3. [O15260-1]
UniGeneiHs.512465.

Genome annotation databases

EnsembliENST00000371989; ENSP00000361057; ENSG00000148248. [O15260-1]
ENST00000485435; ENSP00000419853; ENSG00000148248. [O15260-2]
ENST00000545297; ENSP00000446061; ENSG00000148248. [O15260-3]
ENST00000626303; ENSP00000487537; ENSG00000280951. [O15260-2]
ENST00000629119; ENSP00000486869; ENSG00000280951. [O15260-3]
ENST00000629578; ENSP00000486049; ENSG00000280951. [O15260-1]
GeneIDi6836.
KEGGihsa:6836.
UCSCiuc004cdj.5. human. [O15260-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078866 mRNA. Translation: AAD44498.1.
AF078867 mRNA. Translation: AAD44499.1.
AK300004 mRNA. Translation: BAH13190.1.
AK315488 mRNA. Translation: BAG37872.1.
AL158826 Genomic DNA. Translation: CAI12840.1.
CH471090 Genomic DNA. Translation: EAW88071.1.
CH471090 Genomic DNA. Translation: EAW88074.1.
BC018741 mRNA. Translation: AAH18741.1.
BC111023 mRNA. Translation: AAI11024.1.
Y14820 mRNA. Translation: CAA75099.1.
Y17214 Genomic DNA. Translation: CAA76692.1.
CCDSiCCDS65177.1. [O15260-3]
CCDS65178.1. [O15260-2]
CCDS6968.1. [O15260-1]
RefSeqiNP_001267718.1. NM_001280789.1. [O15260-2]
NP_001267721.1. NM_001280792.1. [O15260-3]
NP_149351.1. NM_033161.3. [O15260-1]
UniGeneiHs.512465.

3D structure databases

ProteinModelPortaliO15260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112703. 53 interactions.
IntActiO15260. 23 interactions.
MINTiMINT-2998714.
STRINGi9606.ENSP00000361057.

PTM databases

iPTMnetiO15260.
PhosphoSiteiO15260.
SwissPalmiO15260.

Polymorphism and mutation databases

BioMutaiSURF4.

Proteomic databases

EPDiO15260.
MaxQBiO15260.
PaxDbiO15260.
PRIDEiO15260.
TopDownProteomicsiO15260-1. [O15260-1]

Protocols and materials databases

DNASUi6836.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371989; ENSP00000361057; ENSG00000148248. [O15260-1]
ENST00000485435; ENSP00000419853; ENSG00000148248. [O15260-2]
ENST00000545297; ENSP00000446061; ENSG00000148248. [O15260-3]
ENST00000626303; ENSP00000487537; ENSG00000280951. [O15260-2]
ENST00000629119; ENSP00000486869; ENSG00000280951. [O15260-3]
ENST00000629578; ENSP00000486049; ENSG00000280951. [O15260-1]
GeneIDi6836.
KEGGihsa:6836.
UCSCiuc004cdj.5. human. [O15260-1]

Organism-specific databases

CTDi6836.
GeneCardsiSURF4.
HGNCiHGNC:11476. SURF4.
MIMi185660. gene.
neXtProtiNX_O15260.
PharmGKBiPA36261.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3998. Eukaryota.
COG2259. LUCA.
GeneTreeiENSGT00530000064123.
HOGENOMiHOG000206959.
HOVERGENiHBG054238.
InParanoidiO15260.
OMAiDQLWYLQ.
OrthoDBiEOG7GTT4K.
PhylomeDBiO15260.
TreeFamiTF300001.

Enzyme and pathway databases

ReactomeiR-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiSURF4. human.
GeneWikiiSURF4.
GenomeRNAii6836.
PROiO15260.
SOURCEiSearch...

Gene expression databases

BgeeiO15260.
CleanExiHS_SURF4.
ExpressionAtlasiO15260. baseline and differential.
GenevisibleiO15260. HS.

Family and domain databases

InterProiIPR002995. Surf4.
[Graphical view]
PfamiPF02077. SURF4. 1 hit.
[Graphical view]
PROSITEiPS01339. SURF4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Pituitary.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  6. "The surf-4 gene encodes a novel 30 kDa integral membrane protein."
    Reeves J.E., Fried M.
    Mol. Membr. Biol. 12:201-208(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-261.
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 258-269.
  8. "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46."
    Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W., Hauri H.-P.
    J. Biol. Chem. 279:47242-47253(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  9. "The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi."
    Mitrovic S., Ben-Tekaya H., Koegler E., Gruenberg J., Hauri H.-P.
    Mol. Biol. Cell 19:1976-1990(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH TMED2 AND TMED10, INTERACTION WITH LMAN1, SUBCELLULAR LOCATION, MUTAGENESIS OF 265-LYS--LYS-267.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSURF4_HUMAN
AccessioniPrimary (citable) accession number: O15260
Secondary accession number(s): B7Z6A4
, O60923, Q5T8U6, Q9UNZ0, Q9UNZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 1, 2000
Last modified: June 8, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.