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O15245 (S22A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 1
Alternative name(s):
Organic cation transporter 1
Short name=hOCT1
Gene names
Name:SLC22A1
Synonyms:OCT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase. Ref.1 Ref.2 Ref.3 Ref.8 Ref.9 Ref.10 Ref.12 Ref.14

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein Ref.11.

Tissue specificity

Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines. Ref.1 Ref.2 Ref.3

Induction

In the liver activated by HNF4A and suppressed by bile acids via NR0B2. Increased by cholesterol treatment in hepatocyte cells. Ref.13 Ref.15

Post-translational modification

Phosphorylated By similarity.

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=1.47 mM for metformin Ref.2 Ref.8 Ref.12

KM=229 µM for TEA

KM=14.6 µM for MPP

Vmax=396 pmol/min/mg enzyme for metformin uptake

Vmax=2.89 nmol/min/mg enzyme for TEA uptake

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdopamine transport

Inferred from electronic annotation. Source: Ensembl

drug transmembrane transport

Traceable author statement. Source: Reactome

epinephrine transport

Inferred from electronic annotation. Source: Ensembl

establishment or maintenance of transmembrane electrochemical gradient

Inferred from electronic annotation. Source: Ensembl

norepinephrine transport

Inferred from electronic annotation. Source: Ensembl

organic cation transport

Traceable author statement Ref.2. Source: UniProtKB

protein homooligomerization

Inferred from electronic annotation. Source: Ensembl

small molecule metabolic process

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentbasolateral plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of plasma membrane

Traceable author statement Ref.2. Source: UniProtKB

membrane

Traceable author statement Ref.2. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionacetylcholine transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

dopamine transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

norepinephrine transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

organic cation transmembrane transporter activity

Traceable author statement Ref.2. Source: UniProtKB

quaternary ammonium group transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

secondary active organic cation transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CERS2Q96G233EBI-1172714,EBI-1057080

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O15245-1)

Also known as: hOCT1G/L554;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O15245-2)

Also known as: hOCT1G/L506;

The sequence of this isoform differs from the canonical sequence as follows:
     462-506: RNLGVMVCSS...ILFAVLGLLA → SGVGPACRGS...SKAQRKHDLP
     507-554: Missing.
Isoform 3 (identifier: O15245-3)

Also known as: hOCT1G483;

The sequence of this isoform differs from the canonical sequence as follows:
     462-532: Missing.
Isoform 4 (identifier: O15245-4)

Also known as: hOCT1G353;

The sequence of this isoform differs from the canonical sequence as follows:
     354-554: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Solute carrier family 22 member 1
PRO_0000333875

Regions

Topological domain1 – 2121Cytoplasmic Potential
Transmembrane22 – 4221Helical; Potential
Topological domain43 – 149107Extracellular Potential
Transmembrane150 – 17021Helical; Potential
Topological domain171 – 1766Cytoplasmic Potential
Transmembrane177 – 19721Helical; Potential
Topological domain198 – 2069Extracellular Potential
Transmembrane207 – 22923Helical; Potential
Topological domain230 – 2356Cytoplasmic Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 2626Extracellular Potential
Transmembrane263 – 28321Helical; Potential
Topological domain284 – 34764Cytoplasmic Potential
Transmembrane348 – 36821Helical; Potential
Topological domain369 – 3768Extracellular Potential
Transmembrane377 – 39721Helical; Potential
Topological domain398 – 4025Cytoplasmic Potential
Transmembrane403 – 42321Helical; Potential
Topological domain424 – 4318Extracellular Potential
Transmembrane432 – 45221Helical; Potential
Topological domain453 – 46412Cytoplasmic Potential
Transmembrane465 – 48521Helical; Potential
Topological domain486 – 4927Extracellular Potential
Transmembrane493 – 51321Helical; Potential
Topological domain514 – 55441Cytoplasmic Potential

Sites

Site4501Involved in affinity and selectivity of cations as well as in translocation By similarity

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence354 – 554201Missing in isoform 4.
VSP_033587
Alternative sequence462 – 53271Missing in isoform 3.
VSP_033588
Alternative sequence462 – 50645RNLGV…LGLLA → SGVGPACRGSDATSSRDQGG RFARDHEGRREPWEKSKAQR KHDLP in isoform 2.
VSP_033589
Alternative sequence507 – 55448Missing in isoform 2.
VSP_033590
Natural variant141S → F Exclusively found in the African American population; increase of the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs34447885 [ dbSNP | Ensembl ].
VAR_043319
Natural variant411F → L. Ref.19
Corresponds to variant rs2297373 [ dbSNP | Ensembl ].
VAR_043320
Natural variant611R → C Reduction of the MPP uptake. Reduction of the MPP uptake; when associated with V-408. Ref.16 Ref.17
Corresponds to variant rs12208357 [ dbSNP | Ensembl ].
VAR_043321
Natural variant851L → F No changes in the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs35546288 [ dbSNP | Ensembl ].
VAR_043322
Natural variant881C → R. Ref.16
Corresponds to variant rs55918055 [ dbSNP | Ensembl ].
VAR_043323
Natural variant1601L → F No changes in both TEA and MPP uptake. No MPP uptake; when associated with S-401. Largely localized in the plasma membrane. Ref.1 Ref.5 Ref.7 Ref.16 Ref.17 Ref.18 Ref.19
Corresponds to variant rs683369 [ dbSNP | Ensembl ].
VAR_043324
Natural variant1891S → L No changes in the MPP uptake. No changes in the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs34104736 [ dbSNP | Ensembl ].
VAR_043325
Natural variant2201G → V No MPP uptake. Reduction of the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs36103319 [ dbSNP | Ensembl ].
VAR_043326
Natural variant2831P → L. Ref.18
Corresponds to variant rs4646277 [ dbSNP | Ensembl ].
VAR_043327
Natural variant2871R → G. Ref.18
Corresponds to variant rs4646278 [ dbSNP | Ensembl ].
VAR_043328
Natural variant3411P → L Reduction of the MPP uptake. Reduction of the MPP uptake; when associated with V-408. Partly reduction of TEA uptake. Largely localized in the plasma membrane. Ref.17 Ref.18 Ref.19
Corresponds to variant rs2282143 [ dbSNP | Ensembl ].
VAR_043329
Natural variant3421R → H No changes in the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs34205214 [ dbSNP | Ensembl ].
VAR_043330
Natural variant4011G → S No MPP uptake. Reduction of the serotonin uptake. No MPP uptake; when associated with L-160. Ref.16 Ref.17
Corresponds to variant rs34130495 [ dbSNP | Ensembl ].
VAR_043331
Natural variant4081M → V No changes in the MPP uptake. No changes in the MPP uptake; when associated with F-14. No changes in the MPP uptake; when associated with F-85. No changes in the MPP uptake; when associated with L-189. No changes in the MPP uptake; when associated with His-342. No changes in the MPP uptake; when associated with M-420 del. No changes in the MPP uptake; when associated with I-440. No changes in the MPP uptake; when associated with I-461. No changes in the MPP uptake; when associated with M-488. Reduction of the MPP uptake; when associated with C-61. No MPP uptake; when associated with V-220. Reduction of the MPP uptake; when associated with L-341. No MPP uptake; when associated with S-401. No MPP uptake; when associated with R-465. Ref.5 Ref.17 Ref.19
Corresponds to variant rs628031 [ dbSNP | Ensembl ].
VAR_043332
Natural variant4201Missing No changes in the MPP uptake. No changes in the MPP uptake; when associated with V-408. Ref.5 Ref.16 Ref.17
VAR_043333
Natural variant4401M → I. Ref.17
Corresponds to variant rs35956182 [ dbSNP | Ensembl ].
VAR_043334
Natural variant4611V → I No changes in the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs34295611 [ dbSNP | Ensembl ].
VAR_043335
Natural variant4651G → R Reduction of the localization to the basolateral membrane. No MPP uptake; when associated with V-408. Ref.5 Ref.17
Corresponds to variant rs34059508 [ dbSNP | Ensembl ].
VAR_043336
Natural variant4881R → M No changes in the MPP uptake; when associated with V-408. Ref.17
Corresponds to variant rs35270274 [ dbSNP | Ensembl ].
VAR_043337

Experimental info

Mutagenesis4651G → A: No changes in the MPP uptake. Ref.17

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (hOCT1G/L554) [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 55206B897DE32202

FASTA55461,154
        10         20         30         40         50         60 
MPTVDDILEQ VGESGWFQKQ AFLILCLLSA AFAPICVGIV FLGFTPDHHC QSPGVAELSQ 

        70         80         90        100        110        120 
RCGWSPAEEL NYTVPGLGPA GEAFLGQCRR YEVDWNQSAL SCVDPLASLA TNRSHLPLGP 

       130        140        150        160        170        180 
CQDGWVYDTP GSSIVTEFNL VCADSWKLDL FQSCLNAGFL FGSLGVGYFA DRFGRKLCLL 

       190        200        210        220        230        240 
GTVLVNAVSG VLMAFSPNYM SMLLFRLLQG LVSKGNWMAG YTLITEFVGS GSRRTVAIMY 

       250        260        270        280        290        300 
QMAFTVGLVA LTGLAYALPH WRWLQLAVSL PTFLFLLYYW CVPESPRWLL SQKRNTEAIK 

       310        320        330        340        350        360 
IMDHIAQKNG KLPPADLKML SLEEDVTEKL SPSFADLFRT PRLRKRTFIL MYLWFTDSVL 

       370        380        390        400        410        420 
YQGLILHMGA TSGNLYLDFL YSALVEIPGA FIALITIDRV GRIYPMAMSN LLAGAACLVM 

       430        440        450        460        470        480 
IFISPDLHWL NIIIMCVGRM GITIAIQMIC LVNAELYPTF VRNLGVMVCS SLCDIGGIIT 

       490        500        510        520        530        540 
PFIVFRLREV WQALPLILFA VLGLLAAGVT LLLPETKGVA LPETMKDAEN LGRKAKPKEN 

       550 
TIYLKVQTSE PSGT 

« Hide

Isoform 2 (hOCT1G/L506) [UniParc].

Checksum: FDD376F7F8547B5D
Show »

FASTA50656,094
Isoform 3 (hOCT1G483) [UniParc].

Checksum: 843CF89CFB1D9827
Show »

FASTA48353,593
Isoform 4 (hOCT1G353) [UniParc].

Checksum: 88073B911A5E5633
Show »

FASTA35339,224

References

« Hide 'large scale' references
[1]"Cloning and characterization of two human polyspecific organic cation transporters."
Gorboulev V., Ulzheimer J.C., Akhoundova A., Ulzheimer-Teuber I., Karbach U., Quester S., Baumann C., Lang F., Busch A.E., Koepsell H.
DNA Cell Biol. 16:871-881(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANT PHE-160.
Tissue: Liver.
[2]"Cloning and functional expression of a human liver organic cation transporter."
Zhang L., Dresser M.J., Gray A.T., Yost S.C., Terashita S., Giacomini K.M.
Mol. Pharmacol. 51:913-921(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[3]"Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1 (hOCT1/SLC22A1)."
Hayer M., Boenisch H., Bruess M.
Ann. Hum. Genet. 63:473-482(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3 AND 4).
[4]Erratum
Hayer M., Bonisch H., Bruss M.
Ann. Hum. Genet. 64:267-267(2000)
Cited for: SEQUENCE REVISION.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS PHE-160; VAL-408; MET-420 DEL AND ARG-465.
Tissue: Caudate nucleus.
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PHE-160.
[8]"Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa)."
Zhang L., Schaner M.E., Giacomini K.M.
J. Pharmacol. Exp. Ther. 286:354-361(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[9]"Comparison of 'type I' and 'type II' organic cation transport by organic cation transporters and organic anion-transporting polypeptides."
van Montfoort J.E., Mueller M., Groothuis G.M.M., Meijer D.K.F., Koepsell H., Meier P.J.
J. Pharmacol. Exp. Ther. 298:110-115(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Regulation of the human organic cation transporter hOCT1."
Ciarimboli G., Struwe K., Arndt P., Gorboulev V., Koepsell H., Schlatter E., Hirsch J.R.
J. Cell. Physiol. 201:420-428(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Drug specificity and intestinal membrane localization of human organic cation transporters (OCT)."
Mueller J., Lips K.S., Metzner L., Neubert R.H.H., Koepsell H., Brandsch M.
Biochem. Pharmacol. 70:1851-1860(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[12]"Metformin is a superior substrate for renal organic cation transporter OCT2 rather than hepatic OCT1."
Kimura N., Masuda S., Tanihara Y., Ueo H., Okuda M., Katsura T., Inui K.
Drug Metab. Pharmacokinet. 20:379-386(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[13]"The human organic cation transporter-1 gene is transactivated by hepatocyte nuclear factor-4alpha."
Saborowski M., Kullak-Ublick G.A., Eloranta J.J.
J. Pharmacol. Exp. Ther. 317:778-785(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[14]"Differential pharmacological in vitro properties of organic cation transporters and regional distribution in rat brain."
Amphoux A., Vialou V., Drescher E., Bruess M., Mannoury La Cour C., Rochat C., Millan M.J., Giros B., Boenisch H., Gautron S.
Neuropharmacology 50:941-952(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"The expression of the solute carriers NTCP and OCT-1 is regulated by cholesterol in HepG2 cells."
Dias V., Ribeiro V.
Fundam. Clin. Pharmacol. 21:445-450(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[16]"Identification of genetic variations of the human organic cation transporter hOCT1 and their functional consequences."
Kerb R., Brinkmann U., Chatskaia N., Gorbunov D., Gorboulev V., Mornhinweg E., Keil A., Eichelbaum M., Koepsell H.
Pharmacogenetics 12:591-595(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS CYS-61; ARG-88; PHE-160; SER-401 AND MET-420 DEL, CHARACTERIZATION OF VARIANTS CYS-61; ARG-88; PHE-160; SER-401 AND MET-420 DEL.
[17]"Evolutionary conservation predicts function of variants of the human organic cation transporter, OCT1."
Shu Y., Leabman M.K., Feng B., Mangravite L.M., Huang C.C., Stryke D., Kawamoto M., Johns S.J., DeYoung J., Carlson E., Ferrin T.E., Herskowitz I., Giacomini K.M.
Proc. Natl. Acad. Sci. U.S.A. 100:5902-5907(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS PHE-14; CYS-61; PHE-85; PHE-160; LEU-189; VAL-220; LEU-341; HIS-342; SER-401; VAL-408; MET-420 DEL; ILE-440; ILE-461; ARG-465 AND MET-488, CHARACTERIZATION OF VARIANTS PHE-14; CYS-61; PHE-85; PHE-160; LEU-189; VAL-220; LEU-341; HIS-342; SER-401; VAL-408; MET-420 DEL; ILE-440; ILE-461; ARG-465 AND MET-488, MUTAGENESIS OF GLY-465.
[18]"Novel single nucleotide polymorphisms of organic cation transporter 1 (SLC22A1) affecting transport functions."
Sakata T., Anzai N., Shin H.J., Noshiro R., Hirata T., Yokoyama H., Kanai Y., Endou H.
Biochem. Biophys. Res. Commun. 313:789-793(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS PHE-160; LEU-283; GLY-287 AND LEU-341.
[19]"Seven novel single nucleotide polymorphisms in the human SLC22A1 gene encoding organic cation transporter 1 (OCT1)."
Itoda M., Saito Y., Maekawa K., Hichiya H., Komamura K., Kamakura S., Kitakaze M., Tomoike H., Ueno K., Ozawa S., Sawada J.
Drug Metab. Pharmacokinet. 19:308-312(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS LEU-41; PHE-160; LEU-341 AND VAL-408.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98332 mRNA. Translation: CAA66977.1.
U77086 mRNA. Translation: AAB67703.1.
AJ243995 expand/collapse EMBL AC list , AJ243996, AJ243998, AJ243999, AJ244000, AJ245460, AJ276051, AJ276052, AJ276053 Genomic DNA. Translation: CAB95971.1.
AK289887 mRNA. Translation: BAF82576.1.
AL353625 Genomic DNA. Translation: CAH72016.1.
BC126364 mRNA. Translation: AAI26365.1.
RefSeqNP_003048.1. NM_003057.2.
NP_694857.1. NM_153187.1.
UniGeneHs.117367.

3D structure databases

ProteinModelPortalO15245.
SMRO15245. Positions 153-518.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112467. 1 interaction.
IntActO15245. 1 interaction.
STRING9606.ENSP00000355930.

Chemistry

BindingDBO15245.
ChEMBLCHEMBL5685.

Protein family/group databases

TCDB2.A.1.19.29. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteO15245.

Proteomic databases

PaxDbO15245.
PRIDEO15245.

Protocols and materials databases

DNASU6580.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000324965; ENSP00000318103; ENSG00000175003. [O15245-2]
ENST00000366963; ENSP00000355930; ENSG00000175003. [O15245-1]
ENST00000457470; ENSP00000409557; ENSG00000175003. [O15245-3]
ENST00000460902; ENSP00000439274; ENSG00000175003. [O15245-4]
GeneID6580.
KEGGhsa:6580.
UCSCuc003qtc.3. human. [O15245-1]
uc003qtd.3. human. [O15245-2]

Organism-specific databases

CTD6580.
GeneCardsGC06P160542.
HGNCHGNC:10963. SLC22A1.
HPAHPA029846.
MIM602607. gene.
neXtProtNX_O15245.
PharmGKBPA329.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000234568.
HOVERGENHBG061545.
InParanoidO15245.
KOK08198.
OMAVCADSWK.
OrthoDBEOG78PV8N.
PhylomeDBO15245.
TreeFamTF315847.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_20679. Amine compound SLC transporters.

Gene expression databases

ArrayExpressO15245.
BgeeO15245.
CleanExHS_SLC22A1.
GenevestigatorO15245.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00898. 2A0119. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSLC22A1.
GenomeRNAi6580.
NextBio25603.
PROO15245.
SOURCESearch...

Entry information

Entry nameS22A1_HUMAN
AccessionPrimary (citable) accession number: O15245
Secondary accession number(s): A6NFF3 expand/collapse secondary AC list , A8K1H2, C9JSU6, O15395, Q9NQD4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 30, 2010
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM