##gff-version 3 O15244 UniProtKB Chain 1 555 . . . ID=PRO_0000320957;Note=Solute carrier family 22 member 2 O15244 UniProtKB Topological domain 1 22 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 23 43 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 44 150 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 151 171 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 172 177 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 178 198 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 199 208 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 209 229 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 230 238 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 239 259 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 260 263 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 264 284 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 285 348 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 349 369 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 370 375 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 376 396 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 397 414 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 415 435 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 436 441 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 442 462 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 463 464 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 465 485 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 486 494 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Transmembrane 495 515 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Topological domain 516 555 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Motif 284 288 . . . Note=Proline-rich sequence;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Site 451 451 . . . Note=Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9R0W2 O15244 UniProtKB Glycosylation 72 72 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255 O15244 UniProtKB Alternative sequence 225 242 . . . ID=VSP_031771;Note=In isoform 3. ITEFVGRRYRRTVGIFYQ->SKNVCACNCENKATSLPK;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:15489334;Dbxref=PMID:15489334 O15244 UniProtKB Alternative sequence 243 555 . . . ID=VSP_031772;Note=In isoform 3. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:15489334;Dbxref=PMID:15489334 O15244 UniProtKB Alternative sequence 427 483 . . . ID=VSP_031773;Note=In isoform 2. DLQWLKIIISCLGRMGITMAYEIVCLVNAELYPTFIRNLGVHICSSMCDIGGIITPF->GKFQVKLESYLQDPGERECHGPLIGKPCNLSSKSIWKDKLEGSIWDPSEQIHMASLL;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:12089365;Dbxref=PMID:12089365 O15244 UniProtKB Alternative sequence 484 555 . . . ID=VSP_031774;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:12089365;Dbxref=PMID:12089365 O15244 UniProtKB Natural variant 54 54 . . . ID=VAR_039322;Note=P->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12142729;Dbxref=dbSNP:rs8177504,PMID:12142729 O15244 UniProtKB Natural variant 165 165 . . . ID=VAR_039323;Note=Lower Vmax for MPP(+) transport%3B no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol. M->I;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12142729,ECO:0000269|PubMed:17460754;Dbxref=dbSNP:rs8177507,PMID:12142729,PMID:17460754 O15244 UniProtKB Natural variant 201 201 . . . ID=VAR_039324;Note=T->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17111267;Dbxref=dbSNP:rs145450955,PMID:17111267 O15244 UniProtKB Natural variant 270 270 . . . ID=VAR_039325;Note=Decreased Ki value for TBA inhibition of MPP(+)%3B no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol. S->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12089365,ECO:0000269|PubMed:12142729,ECO:0000269|PubMed:14702039,ECO:0000269|PubMed:15489334,ECO:0000269|PubMed:17111267,ECO:0000269|PubMed:17460754,ECO:0000269|PubMed:9260930;Dbxref=dbSNP:rs316019,PMID:12089365,PMID:12142729,PMID:14702039,PMID:15489334,PMID:17111267,PMID:17460754,PMID:9260930 O15244 UniProtKB Natural variant 297 297 . . . ID=VAR_039326;Note=A->G;Dbxref=dbSNP:rs8177513 O15244 UniProtKB Natural variant 400 400 . . . ID=VAR_039327;Note=Lower Vmax and reduced Ki value for TBA inhibition of MPP(+)%3B lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro)%3B no change in transport efficiency (Vmax/Km) and clearance of salsolinol. R->C;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12142729,ECO:0000269|PubMed:17460754;Dbxref=dbSNP:rs8177516,PMID:12142729,PMID:17460754 O15244 UniProtKB Natural variant 432 432 . . . ID=VAR_039328;Note=Lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP%3B no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol. K->Q;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12142729,ECO:0000269|PubMed:17460754;Dbxref=dbSNP:rs8177517,PMID:12142729,PMID:17460754 O15244 UniProtKB Natural variant 463 463 . . . ID=VAR_039329;Note=R->K;Dbxref=dbSNP:rs3907239 O15244 UniProtKB Mutagenesis 73 73 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 92 92 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 128 128 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 169 169 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 241 241 . . . Note=Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake%3B when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation%3B when associated with F-362 and F-377. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 257 257 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 279 279 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 280 280 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 284 284 . . . Note=Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation. P->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 286 286 . . . Note=No change in TEA and metformin uptake. No change in tyrosine phosphorylation. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 287 287 . . . Note=Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation. P->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 362 362 . . . Note=Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake%3B when associated with F-241. Strong decrease in TEA uptake%3B when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation%3B when associated with F-241 and F-377. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 377 377 . . . Note=Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake%3B when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation%3B when associated with F-241 and F-362. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 458 458 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622 O15244 UniProtKB Mutagenesis 544 544 . . . Note=No change in TEA uptake. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26979622;Dbxref=PMID:26979622