O15244 (S22A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 22 member 2

Alternative name(s):
Organic cation transporter 2
Short name=hOCT2

Gene names

Name:	SLC22A2
Synonyms:	OCT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, dopamine, noradrenaline (norepinephrine), serotonin, choline, famotidine, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP, amiloride, metformin, N-1-methylnicotinamide (NMN), tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity. Ref.1 Ref.2 Ref.8 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Mainly expressed in kidney. Localized at the luminal membrane and basolateral membrane of kidney distal tubule and proximal tubules. To a lower extent, expressed in neurons of the cerebral cortex and in various subcortical nuclei (at protein levels). Also detected in secretory phase endometrium; in scattered cells in the stroma. Ref.1 Ref.8 Ref.10 Ref.22
Induction	May be down-regulated in diabetic patients. Ref.13
Sequence similarities	Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]
Biophysicochemical properties	Kinetic parameters: KM=1 mM for agmatine Ref.2 Ref.8 Ref.9 Ref.11 Ref.14 Ref.15 Ref.16 Ref.17 KM=95 µM for amiloride KM=24 µM for ASP KM=34 µM for memantine KM=27 µM for amantadine KM=1.38 mM for metformin KM=1.9 mM for noradrenaline KM=1.9 mM for norepinephrine KM=1.3 mM for histamine KM=0.39 mM for dopamine KM=0.08 mM for serotonine KM=72.6 µM for cimetidine (at pH 7.4 and 37 degrees Celsius) KM=56.1 µM for famotidine KM=65.2 µM for ranitidine KM=431 µM for TEA (isoform 1) KM=63 µM for TEA (isoform 2) Vmax=3770 pmol/min/mg enzyme for TEA uptake (isoform 1) Vmax=314 pmol/min/mg enzyme for TEA uptake (isoform 2) Vmax=11.9 nmol/min/mg enzyme for metformin uptake Vmax=2170 pmol/min/mg enzyme for cimetidine uptake Vmax=204 pmol/min/mg enzyme for famotidine uptake Vmax=265 pmol/min/mg enzyme for ranitidine uptake

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	body fluid secretion Traceable author statement Ref.1. Source: ProtInc drug transmembrane transport Traceable author statement. Source: Reactome histamine transport Inferred from electronic annotation. Source: Compara neurotransmitter biosynthetic process Traceable author statement. Source: Reactome neurotransmitter secretion Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	choline transmembrane transporter activity Inferred from electronic annotation. Source: Compara organic cation transmembrane transporter activity Inferred from direct assay PubMed 16024787. Source: MGI secondary active organic cation transmembrane transporter activity Inferred from electronic annotation. Source: Compara steroid binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O15244-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O15244-2)

Also known as: OCT2-A;

The sequence of this isoform differs from the canonical sequence as follows:
     427-483: DLQWLKIIIS...CDIGGIITPF → GKFQVKLESY...SEQIHMASLL
     484-555: Missing.

Isoform 3 (identifier: O15244-3)

The sequence of this isoform differs from the canonical sequence as follows:
     225-242: ITEFVGRRYRRTVGIFYQ → SKNVCACNCENKATSLPK
     243-555: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Solute carrier family 22 member 2		PRO_0000320957
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 43	21	Helical; Potential
	Topological domain	44 – 150	107	Extracellular Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Topological domain	172 – 177	6	Cytoplasmic Potential
	Transmembrane	178 – 198	21	Helical; Potential
	Topological domain	199 – 208	10	Extracellular Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Topological domain	230 – 238	9	Cytoplasmic Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Topological domain	260 – 263	4	Extracellular Potential
	Transmembrane	264 – 284	21	Helical; Potential
	Topological domain	285 – 348	64	Cytoplasmic Potential
	Transmembrane	349 – 369	21	Helical; Potential
	Topological domain	370 – 375	6	Extracellular Potential
	Transmembrane	376 – 396	21	Helical; Potential
	Topological domain	397 – 414	18	Cytoplasmic Potential
	Transmembrane	415 – 435	21	Helical; Potential
	Topological domain	436 – 441	6	Extracellular Potential
	Transmembrane	442 – 462	21	Helical; Potential
	Topological domain	463 – 464	2	Cytoplasmic Potential
	Transmembrane	465 – 485	21	Helical; Potential
	Topological domain	486 – 494	9	Extracellular Potential
	Transmembrane	495 – 515	21	Helical; Potential
	Topological domain	516 – 555	40	Cytoplasmic Potential
Sites
	Site	451	1	Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations By similarity
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	225 – 242	18	ITEFV…GIFYQ → SKNVCACNCENKATSLPK in isoform 3.		VSP_031771
	Alternative sequence	243 – 555	313	Missing in isoform 3.		VSP_031772
	Alternative sequence	427 – 483	57	DLQWL…IITPF → GKFQVKLESYLQDPGERECH GPLIGKPCNLSSKSIWKDKL EGSIWDPSEQIHMASLL in isoform 2.		VSP_031773
	Alternative sequence	484 – 555	72	Missing in isoform 2.		VSP_031774
	Natural variant	54	1	P → S. Ref.23 Corresponds to variant rs8177504 [ dbSNP | Ensembl ].		VAR_039322
	Natural variant	165	1	M → I Lower Vmax. Ref.23		VAR_039323
	Natural variant	201	1	T → M. Ref.24		VAR_039324
	Natural variant	270	1	S → A Increased Ki value for TBA inhibition of MPP. Ref.1 Ref.2 Ref.3 Ref.6 Ref.23 Ref.24 Corresponds to variant rs316019 [ dbSNP | Ensembl ].		VAR_039325
	Natural variant	297	1	A → G. Corresponds to variant rs8177513 [ dbSNP | Ensembl ].		VAR_039326
	Natural variant	400	1	R → C Lower Vmax and reduced Ki value for TBA inhibition of MPP. Ref.23 Corresponds to variant rs8177516 [ dbSNP | Ensembl ].		VAR_039327
	Natural variant	432	1	K → Q Lower Km value for MPP and reduced Ki value for TBA inhibition of MPP. Ref.23 Corresponds to variant rs8177517 [ dbSNP | Ensembl ].		VAR_039328
	Natural variant	463	1	R → K. Corresponds to variant rs3907239 [ dbSNP | Ensembl ].		VAR_039329

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 06528F9519CE211E	FASTA	555	62,581
	10 20 30 40 50 60 MPTTVDDVLE HGGEFHFFQK QMFFLLALLS ATFAPIYVGI VFLGFTPDHR CRSPGVAELS 70 80 90 100 110 120 LRCGWSPAEE LNYTVPGPGP AGEASPRQCR RYEVDWNQST FDCVDPLASL DTNRSRLPLG 130 140 150 160 170 180 PCRDGWVYET PGSSIVTEFN LVCANSWMLD LFQSSVNVGF FIGSMSIGYI ADRFGRKLCL 190 200 210 220 230 240 LTTVLINAAA GVLMAISPTY TWMLIFRLIQ GLVSKAGWLI GYILITEFVG RRYRRTVGIF 250 260 270 280 290 300 YQVAYTVGLL VLAGVAYALP HWRWLQFTVS LPNFFFLLYY WCIPESPRWL ISQNKNAEAM 310 320 330 340 350 360 RIIKHIAKKN GKSLPASLQR LRLEEETGKK LNPSFLDLVR TPQIRKHTMI LMYNWFTSSV 370 380 390 400 410 420 LYQGLIMHMG LAGDNIYLDF FYSALVEFPA AFMIILTIDR IGRRYPWAAS NMVAGAACLA 430 440 450 460 470 480 SVFIPGDLQW LKIIISCLGR MGITMAYEIV CLVNAELYPT FIRNLGVHIC SSMCDIGGII 490 500 510 520 530 540 TPFLVYRLTN IWLELPLMVF GVLGLVAGGL VLLLPETKGK ALPETIEEAE NMQRPRKNKE 550 KMIYLQVQKL DIPLN
	Isoform 2 (OCT2-A) [UniParc]. Checksum: 0AD53D9697B4E7B2	FASTA	483	54,475
	10 20 30 40 50 60 MPTTVDDVLE HGGEFHFFQK QMFFLLALLS ATFAPIYVGI VFLGFTPDHR CRSPGVAELS 70 80 90 100 110 120 LRCGWSPAEE LNYTVPGPGP AGEASPRQCR RYEVDWNQST FDCVDPLASL DTNRSRLPLG 130 140 150 160 170 180 PCRDGWVYET PGSSIVTEFN LVCANSWMLD LFQSSVNVGF FIGSMSIGYI ADRFGRKLCL 190 200 210 220 230 240 LTTVLINAAA GVLMAISPTY TWMLIFRLIQ GLVSKAGWLI GYILITEFVG RRYRRTVGIF 250 260 270 280 290 300 YQVAYTVGLL VLAGVAYALP HWRWLQFTVS LPNFFFLLYY WCIPESPRWL ISQNKNAEAM 310 320 330 340 350 360 RIIKHIAKKN GKSLPASLQR LRLEEETGKK LNPSFLDLVR TPQIRKHTMI LMYNWFTSSV 370 380 390 400 410 420 LYQGLIMHMG LAGDNIYLDF FYSALVEFPA AFMIILTIDR IGRRYPWAAS NMVAGAACLA 430 440 450 460 470 480 SVFIPGGKFQ VKLESYLQDP GERECHGPLI GKPCNLSSKS IWKDKLEGSI WDPSEQIHMA SLL
	Isoform 3 [UniParc]. Checksum: 510EAB0C05C06B43	FASTA	242	26,685
	10 20 30 40 50 60 MPTTVDDVLE HGGEFHFFQK QMFFLLALLS ATFAPIYVGI VFLGFTPDHR CRSPGVAELS 70 80 90 100 110 120 LRCGWSPAEE LNYTVPGPGP AGEASPRQCR RYEVDWNQST FDCVDPLASL DTNRSRLPLG 130 140 150 160 170 180 PCRDGWVYET PGSSIVTEFN LVCANSWMLD LFQSSVNVGF FIGSMSIGYI ADRFGRKLCL 190 200 210 220 230 240 LTTVLINAAA GVLMAISPTY TWMLIFRLIQ GLVSKAGWLI GYILSKNVCA CNCENKATSL PK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of two human polyspecific organic cation transporters." Gorboulev V., Ulzheimer J.C., Akhoundova A., Ulzheimer-Teuber I., Karbach U., Quester S., Baumann C., Lang F., Busch A.E., Koepsell H. DNA Cell Biol. 16:871-881(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION, VARIANT ALA-270. Tissue: Kidney cortex.
[2]	"cDNA cloning, functional characterization, and tissue distribution of an alternatively spliced variant of organic cation transporter hOCT2 predominantly expressed in the human kidney." Urakami Y., Akazawa M., Saito H., Okuda M., Inui K. J. Am. Soc. Nephrol. 13:1703-1710(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, VARIANT ALA-270. Tissue: Kidney.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-270. Tissue: Kidney.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT ALA-270. Tissue: Colon and Kidney.
[7]	"Gene structures of the human non-neuronal monoamine transporters EMT and OCT2." Gruendemann D., Schoemig E. Hum. Genet. 106:627-635(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-138.
[8]	"Human neurons express the polyspecific cation transporter hOCT2, which translocates monoamine neurotransmitters, amantadine, and memantine." Busch A.E., Karbach U., Miska D., Gorboulev V., Akhoundova A., Volk C., Arndt P., Ulzheimer J.C., Sonders M.S., Baumann C., Waldegger S., Lang F., Koepsell H. Mol. Pharmacol. 54:342-352(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[9]	"Distinct characteristics of organic cation transporters, OCT1 and OCT2, in the basolateral membrane of renal tubules." Urakami Y., Okuda M., Masuda S., Akazawa M., Saito H., Inui K. Pharm. Res. 18:1528-1534(2001) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[10]	"Gene expression levels and immunolocalization of organic ion transporters in the human kidney." Motohashi H., Sakurai Y., Saito H., Masuda S., Urakami Y., Goto M., Fukatsu A., Ogawa O., Inui K. J. Am. Soc. Nephrol. 13:866-874(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[11]	"Agmatine is efficiently transported by non-neuronal monoamine transporters extraneuronal monoamine transporter (EMT) and organic cation transporter 2 (OCT2)." Grundemann D., Hahne C., Berkels R., Schomig E. J. Pharmacol. Exp. Ther. 304:810-817(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[12]	"Different transport properties between famotidine and cimetidine by human renal organic ion transporters (SLC22A)." Motohashi H., Uwai Y., Hiramoto K., Okuda M., Inui K. Eur. J. Pharmacol. 503:25-30(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Cisplatin nephrotoxicity is critically mediated via the human organic cation transporter 2." Ciarimboli G., Ludwig T., Lang D., Pavenstaedt H., Koepsell H., Piechota H.J., Haier J., Jaehde U., Zisowsky J., Schlatter E. Am. J. Pathol. 167:1477-1484(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[14]	"Metformin is a superior substrate for renal organic cation transporter OCT2 rather than hepatic OCT1." Kimura N., Masuda S., Tanihara Y., Ueo H., Okuda M., Katsura T., Inui K. Drug Metab. Pharmacokinet. 20:379-386(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[15]	"A species difference in the transport activities of H2 receptor antagonists by rat and human renal organic anion and cation transporters." Tahara H., Kusuhara H., Endou H., Koepsell H., Imaoka T., Fuse E., Sugiyama Y. J. Pharmacol. Exp. Ther. 315:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[16]	"Metformin transport by renal basolateral organic cation transporter hOCT2." Kimura N., Okuda M., Inui K. Pharm. Res. 22:255-259(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[17]	"Characterization of regulatory mechanisms and states of human organic cation transporter 2." Biermann J., Lang D., Gorboulev V., Koepsell H., Sindic A., Schroter R., Zvirbliene A., Pavenstadt H., Schlatter E., Ciarimboli G. Am. J. Physiol. 290:C1521-C1531(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[18]	"Organic cation transporters are determinants of oxaliplatin cytotoxicity." Zhang S., Lovejoy K.S., Shima J.E., Lagpacan L.L., Shu Y., Lapuk A., Chen Y., Komori T., Gray J.W., Chen X., Lippard S.J., Giacomini K.M. Cancer Res. 66:8847-8857(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[19]	"Interactions of fluoroquinolone antibacterials, DX-619 and levofloxacin, with creatinine transport by renal organic cation transporter hOCT2." Okuda M., Kimura N., Inui K. Drug Metab. Pharmacokinet. 21:432-436(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[20]	"Cisplatin and oxaliplatin, but not carboplatin and nedaplatin, are substrates for human organic cation transporters (SLC22A1-3 and multidrug and toxin extrusion family)." Yonezawa A., Masuda S., Yokoo S., Katsura T., Inui K. J. Pharmacol. Exp. Ther. 319:879-886(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[21]	"Differential contribution of organic cation transporters, OCT2 and MATE1, in platinum agent-induced nephrotoxicity." Yokoo S., Yonezawa A., Masuda S., Fukatsu A., Katsura T., Inui K. Biochem. Pharmacol. 74:477-487(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[22]	"The organic cation transporters (OCT1, OCT2, EMT) and the plasma membrane monoamine transporter (PMAT) show differential distribution and cyclic expression pattern in human endometrium and early pregnancy decidua." Bottalico B., Noskova V., Pilka R., Larsson I., Domanski H., Casslen B., Hansson S.R. Mol. Reprod. Dev. 74:1303-1311(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[23]	"Polymorphisms in a human kidney xenobiotic transporter, OCT2, exhibit altered function." Leabman M.K., Huang C.C., Kawamoto M., Johns S.J., Stryke D., Ferrin T.E., DeYoung J., Taylor T., Clark A.G., Herskowitz I., Giacomini K.M. Pharmacogenetics 12:395-405(2002) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT SER-54, CHARACTERIZATION OF VARIANTS ILE-165; ALA-270; CYS-400 AND GLN-432.
[24]	"Human organic cation transporter (OCT1 and OCT2) gene polymorphisms and therapeutic effects of metformin." Shikata E., Yamamoto R., Takane H., Shigemasa C., Ikeda T., Otsubo K., Ieiri I. J. Hum. Genet. 52:117-122(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS MET-201 AND ALA-270.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98333 mRNA. Translation: CAA66978.1. AB075951 mRNA. Translation: BAC02720.1. AK290787 mRNA. Translation: BAF83476.1. AL162582 Genomic DNA. Translation: CAI20003.1. CH471051 Genomic DNA. Translation: EAW47602.1. BC030978 mRNA. Translation: AAH30978.1. BC039899 mRNA. Translation: AAH39899.1. AJ251885 Genomic DNA. Translation: CAB96133.1.
IPI	IPI00017625. IPI00448803. IPI00885027.
RefSeq	NP_003049.2. NM_003058.3.
UniGene	Hs.436385.
3D structure databases
ProteinModelPortal	O15244.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15244. 8 interactions.
STRING	9606.ENSP00000355920.
PTM databases
PhosphoSite	O15244.
Proteomic databases
PaxDb	O15244.
PRIDE	O15244.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000366953; ENSP00000355920; ENSG00000112499.
GeneID	6582.
KEGG	hsa:6582.
UCSC	uc003qtf.3. human. uc003qth.2. human.
Organism-specific databases
CTD	6582.
GeneCards	GC06M160592.
H-InvDB	HIX0025014.
HGNC	HGNC:10966. SLC22A2.
HPA	HPA008567.
MIM	602608. gene.
neXtProt	NX_O15244.
PharmGKB	PA331.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000234568.
HOVERGEN	HBG061545.
InParanoid	O15244.
KO	K08199.
OMA	KAGWLIS.
OrthoDB	EOG4S4PG6.
PhylomeDB	O15244.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_13685. Neuronal System. REACT_15518. Transmembrane transport of small molecules. REACT_20679. Amine compound SLC transporters.
Gene expression databases
ArrayExpress	O15244.
Bgee	O15244.
CleanEx	HS_SLC22A2.
Genevestigator	O15244.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004749. Orgcat_transp. IPR005828. Sub_transporter. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00898. 2A0119. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 2 hits. PS00217. SUGAR_TRANSPORT_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1743122.
GenomeRNAi	6582.
NextBio	25613.
SOURCE	Search...

Entry information

Entry name

S22A2_HUMAN

Accession

Primary (citable) accession number: O15244
Secondary accession number(s): Q5T7Q6 , Q6PIQ8, Q8NG62, Q9NQB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.