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O15240 (VGF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurosecretory protein VGF

Cleaved into the following 3 chains:

  1. Neuroendocrine regulatory peptide-1
    Short name=NERP-1
  2. Neuroendocrine regulatory peptide-2
    Short name=NERP-2
  3. Antimicrobial peptide VGF[554-577]
Gene names
Name:VGF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system By similarity. Ref.5 Ref.6

NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. Ref.5 Ref.6

Antimicrobial peptide VGF[554-577]: Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris. Ref.5 Ref.6

Subcellular location

Secreted. Cytoplasmic vesiclesecretory vesicle. Note: Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. Ref.6

Tissue specificity

Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. Ref.6

Post-translational modification

Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure By similarity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 615593Neurosecretory protein VGF
PRO_0000022655
Peptide281 – 30626Neuroendocrine regulatory peptide-1
PRO_0000403364
Peptide310 – 34738Neuroendocrine regulatory peptide-2
PRO_0000403365
Peptide554 – 57724Antimicrobial peptide VGF[554-577] Ref.5
PRO_0000422072

Regions

Compositional bias353 – 44795Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue3101Pyrrolidone carboxylic acid
Modified residue5771Proline amide Ref.5

Experimental info

Sequence conflict129 – 1313PES → AGE in CAA73210. Ref.1
Sequence conflict2821P → A in CAA73210. Ref.1
Sequence conflict3871E → EA in CAA73210. Ref.1
Sequence conflict3931E → D in CAA73210. Ref.1
Sequence conflict4811K → N in CAA73210. Ref.1
Sequence conflict4851N → K in CAA73210. Ref.1
Sequence conflict512 – 5154APAP → PPS in CAA73210. Ref.1
Sequence conflict585 – 5862RA → HAQ in CAA73210. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O15240 [UniParc].

Last modified September 23, 2008. Version 2.
Checksum: 198097C5622AC087

FASTA61567,258
        10         20         30         40         50         60 
MKALRLSASA LFCLLLINGL GAAPPGRPEA QPPPLSSEHK EPVAGDAVPG PKDGSAPEVR 

        70         80         90        100        110        120 
GARNSEPQDE GELFQGVDPR ALAAVLLQAL DRPASPPAPS GSQQGPEEEA AEALLTETVR 

       130        140        150        160        170        180 
SQTHSLPAPE SPEPAAPPRP QTPENGPEAS DPSEELEALA SLLQELRDFS PSSAKRQQET 

       190        200        210        220        230        240 
AAAETETRTH TLTRVNLESP GPERVWRASW GEFQARVPER APLPPPAPSQ FQARMPDSGP 

       250        260        270        280        290        300 
LPETHKFGEG VSSPKTHLGE ALAPLSKAYQ GVAAPFPKAR RPESALLGGS EAGERLLQQG 

       310        320        330        340        350        360 
LAQVEAGRRQ AEATRQAAAQ EERLADLASD LLLQYLLQGG ARQRGLGGRG LQEAAEERES 

       370        380        390        400        410        420 
AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ NALLFAEEED GEAGAEDKRS 

       430        440        450        460        470        480 
QEETPGHRRK EAEGTEEGGE EEDDEEMDPQ TIDSLIELST KLHLPADDVV SIIEEVEEKR 

       490        500        510        520        530        540 
KRKKNAPPEP VPPPRAAPAP THVRSPQPPP PAPAPARDEL PDWNEVLPPW DREEDEVYPP 

       550        560        570        580        590        600 
GPYHPFPNYI RPRTLQPPSA LRRRHYHHAL PPSRHYPGRE AQARRAQEEA EAEERRLQEQ 

       610 
EELENYIEHV LLRRP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, structural organization analysis and chromosomal assignment of the human gene for neurosecretory protein VGF."
Canu N., Possenti R., Ricco A.S., Rocchi M., Levi A.
Genomics 45:443-446(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[2]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: PNS.
[5]"Large-scale identification of endogenous secretory peptides using electron transfer dissociation mass spectrometry."
Sasaki K., Osaki T., Minamino N.
Mol. Cell. Proteomics 12:700-709(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 554-577, FUNCTION, AMIDATION AT PRO-577.
[6]"Neuroendocrine regulatory peptide-1 and -2: novel bioactive peptides processed from VGF."
Toshinai K., Nakazato M.
Cell. Mol. Life Sci. 66:1939-1945(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF PEPTIDES NERP-1 AND NERP-2, PYROGLUTAMATE FORMATION AT GLN-310, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y12661 Genomic DNA. Translation: CAA73210.1.
AC004876 Genomic DNA. Translation: AAD45830.1.
CH471197 Genomic DNA. Translation: EAW50201.1.
BC063835 mRNA. Translation: AAH63835.1.
CCDSCCDS5712.1.
RefSeqNP_003369.2. NM_003378.3.
XP_005250617.1. XM_005250560.2.
XP_005250618.1. XM_005250561.2.
UniGeneHs.587325.

3D structure databases

ProteinModelPortalO15240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113268. 4 interactions.
STRING9606.ENSP00000249330.

PTM databases

PhosphoSiteO15240.

Proteomic databases

MaxQBO15240.
PaxDbO15240.
PeptideAtlasO15240.
PRIDEO15240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000249330; ENSP00000249330; ENSG00000128564.
ENST00000445482; ENSP00000400884; ENSG00000128564.
GeneID7425.
KEGGhsa:7425.
UCSCuc003uxx.4. human.

Organism-specific databases

CTD7425.
GeneCardsGC07M100805.
HGNCHGNC:12684. VGF.
HPAHPA055177.
MIM602186. gene.
neXtProtNX_O15240.
PharmGKBPA37305.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG75774.
HOGENOMHOG000120127.
HOVERGENHBG006806.
InParanoidO15240.
OMALPEAHQF.
OrthoDBEOG7W9RV0.
PhylomeDBO15240.
TreeFamTF338498.

Gene expression databases

BgeeO15240.
CleanExHS_VGF.
GenevestigatorO15240.

Family and domain databases

InterProIPR026128. VGF.
[Graphical view]
PANTHERPTHR15159. PTHR15159. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi7425.
NextBio29086.
PROO15240.
SOURCESearch...

Entry information

Entry nameVGF_HUMAN
AccessionPrimary (citable) accession number: O15240
Secondary accession number(s): Q9UDW8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM