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O15240

- VGF_HUMAN

UniProt

O15240 - VGF_HUMAN

Protein

Neurosecretory protein VGF

Gene

VGF

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.By similarity
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
    Antimicrobial peptide VGF[554-577]: Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris.

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. generation of precursor metabolites and energy Source: Ensembl
    3. glucose homeostasis Source: Ensembl
    4. insulin secretion Source: Ensembl
    5. ovarian follicle development Source: Ensembl
    6. response to cAMP Source: UniProtKB
    7. response to cold Source: Ensembl
    8. response to dietary excess Source: Ensembl
    9. response to insulin Source: Ensembl
    10. sexual reproduction Source: Ensembl

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Growth factor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurosecretory protein VGF
    Cleaved into the following 3 chains:
    Gene namesi
    Name:VGF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:12684. VGF.

    Subcellular locationi

    Secreted 1 Publication. Cytoplasmic vesiclesecretory vesicle 1 Publication
    Note: Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: UniProtKB
    2. extracellular space Source: UniProtKB
    3. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37305.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 615593Neurosecretory protein VGFPRO_0000022655Add
    BLAST
    Peptidei281 – 30626Neuroendocrine regulatory peptide-1PRO_0000403364Add
    BLAST
    Peptidei310 – 34738Neuroendocrine regulatory peptide-2PRO_0000403365Add
    BLAST
    Peptidei554 – 57724Antimicrobial peptide VGF[554-577]PRO_0000422072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei310 – 3101Pyrrolidone carboxylic acid1 Publication
    Modified residuei577 – 5771Proline amide1 Publication

    Post-translational modificationi

    Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.By similarity

    Keywords - PTMi

    Amidation, Cleavage on pair of basic residues, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiO15240.
    PaxDbiO15240.
    PeptideAtlasiO15240.
    PRIDEiO15240.

    PTM databases

    PhosphoSiteiO15240.

    Expressioni

    Tissue specificityi

    Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.1 Publication

    Gene expression databases

    BgeeiO15240.
    CleanExiHS_VGF.
    GenevestigatoriO15240.

    Organism-specific databases

    HPAiHPA055177.

    Interactioni

    Protein-protein interaction databases

    BioGridi113268. 4 interactions.
    IntActiO15240. 2 interactions.
    STRINGi9606.ENSP00000249330.

    Structurei

    3D structure databases

    ProteinModelPortaliO15240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi353 – 44795Asp/Glu-rich (acidic)Add
    BLAST

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG75774.
    HOGENOMiHOG000120127.
    HOVERGENiHBG006806.
    InParanoidiO15240.
    OMAiLPEAHQF.
    OrthoDBiEOG7W9RV0.
    PhylomeDBiO15240.
    TreeFamiTF338498.

    Family and domain databases

    InterProiIPR026128. VGF.
    [Graphical view]
    PANTHERiPTHR15159. PTHR15159. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O15240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKALRLSASA LFCLLLINGL GAAPPGRPEA QPPPLSSEHK EPVAGDAVPG    50
    PKDGSAPEVR GARNSEPQDE GELFQGVDPR ALAAVLLQAL DRPASPPAPS 100
    GSQQGPEEEA AEALLTETVR SQTHSLPAPE SPEPAAPPRP QTPENGPEAS 150
    DPSEELEALA SLLQELRDFS PSSAKRQQET AAAETETRTH TLTRVNLESP 200
    GPERVWRASW GEFQARVPER APLPPPAPSQ FQARMPDSGP LPETHKFGEG 250
    VSSPKTHLGE ALAPLSKAYQ GVAAPFPKAR RPESALLGGS EAGERLLQQG 300
    LAQVEAGRRQ AEATRQAAAQ EERLADLASD LLLQYLLQGG ARQRGLGGRG 350
    LQEAAEERES AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ 400
    NALLFAEEED GEAGAEDKRS QEETPGHRRK EAEGTEEGGE EEDDEEMDPQ 450
    TIDSLIELST KLHLPADDVV SIIEEVEEKR KRKKNAPPEP VPPPRAAPAP 500
    THVRSPQPPP PAPAPARDEL PDWNEVLPPW DREEDEVYPP GPYHPFPNYI 550
    RPRTLQPPSA LRRRHYHHAL PPSRHYPGRE AQARRAQEEA EAEERRLQEQ 600
    EELENYIEHV LLRRP 615
    Length:615
    Mass (Da):67,258
    Last modified:September 23, 2008 - v2
    Checksum:i198097C5622AC087
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1313PES → AGE in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti282 – 2821P → A in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti387 – 3871E → EA in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti393 – 3931E → D in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti481 – 4811K → N in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti485 – 4851N → K in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti512 – 5154APAP → PPS in CAA73210. (PubMed:9344675)Curated
    Sequence conflicti585 – 5862RA → HAQ in CAA73210. (PubMed:9344675)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y12661 Genomic DNA. Translation: CAA73210.1.
    AC004876 Genomic DNA. Translation: AAD45830.1.
    CH471197 Genomic DNA. Translation: EAW50201.1.
    BC063835 mRNA. Translation: AAH63835.1.
    CCDSiCCDS5712.1.
    RefSeqiNP_003369.2. NM_003378.3.
    XP_005250617.1. XM_005250560.2.
    XP_005250618.1. XM_005250561.2.
    UniGeneiHs.587325.

    Genome annotation databases

    EnsembliENST00000249330; ENSP00000249330; ENSG00000128564.
    ENST00000445482; ENSP00000400884; ENSG00000128564.
    GeneIDi7425.
    KEGGihsa:7425.
    UCSCiuc003uxx.4. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y12661 Genomic DNA. Translation: CAA73210.1 .
    AC004876 Genomic DNA. Translation: AAD45830.1 .
    CH471197 Genomic DNA. Translation: EAW50201.1 .
    BC063835 mRNA. Translation: AAH63835.1 .
    CCDSi CCDS5712.1.
    RefSeqi NP_003369.2. NM_003378.3.
    XP_005250617.1. XM_005250560.2.
    XP_005250618.1. XM_005250561.2.
    UniGenei Hs.587325.

    3D structure databases

    ProteinModelPortali O15240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113268. 4 interactions.
    IntActi O15240. 2 interactions.
    STRINGi 9606.ENSP00000249330.

    PTM databases

    PhosphoSitei O15240.

    Proteomic databases

    MaxQBi O15240.
    PaxDbi O15240.
    PeptideAtlasi O15240.
    PRIDEi O15240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000249330 ; ENSP00000249330 ; ENSG00000128564 .
    ENST00000445482 ; ENSP00000400884 ; ENSG00000128564 .
    GeneIDi 7425.
    KEGGi hsa:7425.
    UCSCi uc003uxx.4. human.

    Organism-specific databases

    CTDi 7425.
    GeneCardsi GC07M100805.
    HGNCi HGNC:12684. VGF.
    HPAi HPA055177.
    MIMi 602186. gene.
    neXtProti NX_O15240.
    PharmGKBi PA37305.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG75774.
    HOGENOMi HOG000120127.
    HOVERGENi HBG006806.
    InParanoidi O15240.
    OMAi LPEAHQF.
    OrthoDBi EOG7W9RV0.
    PhylomeDBi O15240.
    TreeFami TF338498.

    Miscellaneous databases

    GenomeRNAii 7425.
    NextBioi 29086.
    PROi O15240.
    SOURCEi Search...

    Gene expression databases

    Bgeei O15240.
    CleanExi HS_VGF.
    Genevestigatori O15240.

    Family and domain databases

    InterProi IPR026128. VGF.
    [Graphical view ]
    PANTHERi PTHR15159. PTHR15159. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, structural organization analysis and chromosomal assignment of the human gene for neurosecretory protein VGF."
      Canu N., Possenti R., Ricco A.S., Rocchi M., Levi A.
      Genomics 45:443-446(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Placenta.
    2. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: PNS.
    5. "Large-scale identification of endogenous secretory peptides using electron transfer dissociation mass spectrometry."
      Sasaki K., Osaki T., Minamino N.
      Mol. Cell. Proteomics 12:700-709(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 554-577, FUNCTION, AMIDATION AT PRO-577.
    6. "Neuroendocrine regulatory peptide-1 and -2: novel bioactive peptides processed from VGF."
      Toshinai K., Nakazato M.
      Cell. Mol. Life Sci. 66:1939-1945(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF PEPTIDES NERP-1 AND NERP-2, PYROGLUTAMATE FORMATION AT GLN-310, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiVGF_HUMAN
    AccessioniPrimary (citable) accession number: O15240
    Secondary accession number(s): Q9UDW8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3