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Protein

Neurosecretory protein VGF

Gene

VGF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.By similarity
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
Antimicrobial peptide VGF[554-577]: Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Neurosecretory protein VGF
Cleaved into the following 3 chains:
Gene namesi
Name:VGF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:12684. VGF.

Subcellular locationi

  • Secreted 1 Publication
  • Cytoplasmic vesiclesecretory vesicle 1 Publication

  • Note: Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.

GO - Cellular componenti

  • cytoplasmic vesicle Source: UniProtKB
  • extracellular space Source: UniProtKB
  • transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37305.

Polymorphism and mutation databases

BioMutaiVGF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 615593Neurosecretory protein VGFPRO_0000022655Add
BLAST
Peptidei281 – 30626Neuroendocrine regulatory peptide-1PRO_0000403364Add
BLAST
Peptidei310 – 34738Neuroendocrine regulatory peptide-2PRO_0000403365Add
BLAST
Peptidei554 – 57724Antimicrobial peptide VGF[554-577]PRO_0000422072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei310 – 3101Pyrrolidone carboxylic acid1 Publication
Modified residuei577 – 5771Proline amide1 Publication

Post-translational modificationi

Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.By similarity

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiO15240.
PaxDbiO15240.
PeptideAtlasiO15240.
PRIDEiO15240.

PTM databases

PhosphoSiteiO15240.

Expressioni

Tissue specificityi

Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.1 Publication

Gene expression databases

BgeeiO15240.
CleanExiHS_VGF.
ExpressionAtlasiO15240. baseline and differential.
GenevisibleiO15240. HS.

Organism-specific databases

HPAiHPA055177.

Interactioni

Protein-protein interaction databases

BioGridi113268. 4 interactions.
IntActiO15240. 2 interactions.
STRINGi9606.ENSP00000249330.

Structurei

3D structure databases

ProteinModelPortaliO15240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi353 – 44795Asp/Glu-rich (acidic)Add
BLAST

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG75774.
GeneTreeiENSGT00390000017745.
HOGENOMiHOG000120127.
HOVERGENiHBG006806.
InParanoidiO15240.
OMAiNYIEHVL.
OrthoDBiEOG7W9RV0.
PhylomeDBiO15240.
TreeFamiTF338498.

Family and domain databases

InterProiIPR026128. VGF.
[Graphical view]
PANTHERiPTHR15159. PTHR15159. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O15240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALRLSASA LFCLLLINGL GAAPPGRPEA QPPPLSSEHK EPVAGDAVPG
60 70 80 90 100
PKDGSAPEVR GARNSEPQDE GELFQGVDPR ALAAVLLQAL DRPASPPAPS
110 120 130 140 150
GSQQGPEEEA AEALLTETVR SQTHSLPAPE SPEPAAPPRP QTPENGPEAS
160 170 180 190 200
DPSEELEALA SLLQELRDFS PSSAKRQQET AAAETETRTH TLTRVNLESP
210 220 230 240 250
GPERVWRASW GEFQARVPER APLPPPAPSQ FQARMPDSGP LPETHKFGEG
260 270 280 290 300
VSSPKTHLGE ALAPLSKAYQ GVAAPFPKAR RPESALLGGS EAGERLLQQG
310 320 330 340 350
LAQVEAGRRQ AEATRQAAAQ EERLADLASD LLLQYLLQGG ARQRGLGGRG
360 370 380 390 400
LQEAAEERES AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ
410 420 430 440 450
NALLFAEEED GEAGAEDKRS QEETPGHRRK EAEGTEEGGE EEDDEEMDPQ
460 470 480 490 500
TIDSLIELST KLHLPADDVV SIIEEVEEKR KRKKNAPPEP VPPPRAAPAP
510 520 530 540 550
THVRSPQPPP PAPAPARDEL PDWNEVLPPW DREEDEVYPP GPYHPFPNYI
560 570 580 590 600
RPRTLQPPSA LRRRHYHHAL PPSRHYPGRE AQARRAQEEA EAEERRLQEQ
610
EELENYIEHV LLRRP
Length:615
Mass (Da):67,258
Last modified:September 23, 2008 - v2
Checksum:i198097C5622AC087
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1313PES → AGE in CAA73210 (PubMed:9344675).Curated
Sequence conflicti282 – 2821P → A in CAA73210 (PubMed:9344675).Curated
Sequence conflicti387 – 3871E → EA in CAA73210 (PubMed:9344675).Curated
Sequence conflicti393 – 3931E → D in CAA73210 (PubMed:9344675).Curated
Sequence conflicti481 – 4811K → N in CAA73210 (PubMed:9344675).Curated
Sequence conflicti485 – 4851N → K in CAA73210 (PubMed:9344675).Curated
Sequence conflicti512 – 5154APAP → PPS in CAA73210 (PubMed:9344675).Curated
Sequence conflicti585 – 5862RA → HAQ in CAA73210 (PubMed:9344675).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12661 Genomic DNA. Translation: CAA73210.1.
AC004876 Genomic DNA. Translation: AAD45830.1.
CH471197 Genomic DNA. Translation: EAW50201.1.
BC063835 mRNA. Translation: AAH63835.1.
CCDSiCCDS5712.1.
RefSeqiNP_003369.2. NM_003378.3.
XP_005250618.1. XM_005250561.3.
XP_011514850.1. XM_011516548.1.
XP_011514851.1. XM_011516549.1.
UniGeneiHs.587325.

Genome annotation databases

EnsembliENST00000249330; ENSP00000249330; ENSG00000128564.
ENST00000445482; ENSP00000400884; ENSG00000128564.
GeneIDi7425.
KEGGihsa:7425.
UCSCiuc003uxx.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12661 Genomic DNA. Translation: CAA73210.1.
AC004876 Genomic DNA. Translation: AAD45830.1.
CH471197 Genomic DNA. Translation: EAW50201.1.
BC063835 mRNA. Translation: AAH63835.1.
CCDSiCCDS5712.1.
RefSeqiNP_003369.2. NM_003378.3.
XP_005250618.1. XM_005250561.3.
XP_011514850.1. XM_011516548.1.
XP_011514851.1. XM_011516549.1.
UniGeneiHs.587325.

3D structure databases

ProteinModelPortaliO15240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113268. 4 interactions.
IntActiO15240. 2 interactions.
STRINGi9606.ENSP00000249330.

PTM databases

PhosphoSiteiO15240.

Polymorphism and mutation databases

BioMutaiVGF.

Proteomic databases

MaxQBiO15240.
PaxDbiO15240.
PeptideAtlasiO15240.
PRIDEiO15240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249330; ENSP00000249330; ENSG00000128564.
ENST00000445482; ENSP00000400884; ENSG00000128564.
GeneIDi7425.
KEGGihsa:7425.
UCSCiuc003uxx.4. human.

Organism-specific databases

CTDi7425.
GeneCardsiGC07M100805.
HGNCiHGNC:12684. VGF.
HPAiHPA055177.
MIMi602186. gene.
neXtProtiNX_O15240.
PharmGKBiPA37305.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG75774.
GeneTreeiENSGT00390000017745.
HOGENOMiHOG000120127.
HOVERGENiHBG006806.
InParanoidiO15240.
OMAiNYIEHVL.
OrthoDBiEOG7W9RV0.
PhylomeDBiO15240.
TreeFamiTF338498.

Miscellaneous databases

GenomeRNAii7425.
NextBioi29086.
PROiO15240.
SOURCEiSearch...

Gene expression databases

BgeeiO15240.
CleanExiHS_VGF.
ExpressionAtlasiO15240. baseline and differential.
GenevisibleiO15240. HS.

Family and domain databases

InterProiIPR026128. VGF.
[Graphical view]
PANTHERiPTHR15159. PTHR15159. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, structural organization analysis and chromosomal assignment of the human gene for neurosecretory protein VGF."
    Canu N., Possenti R., Ricco A.S., Rocchi M., Levi A.
    Genomics 45:443-446(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: PNS.
  5. "Large-scale identification of endogenous secretory peptides using electron transfer dissociation mass spectrometry."
    Sasaki K., Osaki T., Minamino N.
    Mol. Cell. Proteomics 12:700-709(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 554-577, FUNCTION, AMIDATION AT PRO-577.
  6. "Neuroendocrine regulatory peptide-1 and -2: novel bioactive peptides processed from VGF."
    Toshinai K., Nakazato M.
    Cell. Mol. Life Sci. 66:1939-1945(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PEPTIDES NERP-1 AND NERP-2, PYROGLUTAMATE FORMATION AT GLN-310, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVGF_HUMAN
AccessioniPrimary (citable) accession number: O15240
Secondary accession number(s): Q9UDW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: September 23, 2008
Last modified: July 22, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.