O15240 (VGF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurosecretory protein VGF

Cleaved into the following 2 chains:

1. Neuroendocrine regulatory peptide-1
   Short name=NERP-1
2. Neuroendocrine regulatory peptide-2
   Short name=NERP-2

Gene names

Name:

VGF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system By similarity. Ref.5 NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. Ref.5
Subcellular location	Secreted. Cytoplasmic vesicle › secretory vesicle. Note: Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. Ref.5
Tissue specificity	Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. Ref.5
Post-translational modification	Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure By similarity.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to cAMP Inferred from expression pattern. Source: UniProtKB
Cellular component	extracellular space Inferred from direct assay. Source: UniProtKB transport vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 615	593	Neurosecretory protein VGF		PRO_0000022655
	Peptide	281 – 306	26	Neuroendocrine regulatory peptide-1		PRO_0000403364
	Peptide	310 – 347	38	Neuroendocrine regulatory peptide-2		PRO_0000403365
Regions
	Compositional bias	353 – 447	95	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	310	1	Pyrrolidone carboxylic acid
Experimental info
	Sequence conflict	129 – 131	3	PES → AGE in CAA73210. Ref.1
	Sequence conflict	282	1	P → A in CAA73210. Ref.1
	Sequence conflict	387	1	E → EA in CAA73210. Ref.1
	Sequence conflict	393	1	E → D in CAA73210. Ref.1
	Sequence conflict	481	1	K → N in CAA73210. Ref.1
	Sequence conflict	485	1	N → K in CAA73210. Ref.1
	Sequence conflict	512 – 515	4	APAP → PPS in CAA73210. Ref.1
	Sequence conflict	585 – 586	2	RA → HAQ in CAA73210. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O15240 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: 198097C5622AC087	FASTA	615	67,258
	10 20 30 40 50 60 MKALRLSASA LFCLLLINGL GAAPPGRPEA QPPPLSSEHK EPVAGDAVPG PKDGSAPEVR 70 80 90 100 110 120 GARNSEPQDE GELFQGVDPR ALAAVLLQAL DRPASPPAPS GSQQGPEEEA AEALLTETVR 130 140 150 160 170 180 SQTHSLPAPE SPEPAAPPRP QTPENGPEAS DPSEELEALA SLLQELRDFS PSSAKRQQET 190 200 210 220 230 240 AAAETETRTH TLTRVNLESP GPERVWRASW GEFQARVPER APLPPPAPSQ FQARMPDSGP 250 260 270 280 290 300 LPETHKFGEG VSSPKTHLGE ALAPLSKAYQ GVAAPFPKAR RPESALLGGS EAGERLLQQG 310 320 330 340 350 360 LAQVEAGRRQ AEATRQAAAQ EERLADLASD LLLQYLLQGG ARQRGLGGRG LQEAAEERES 370 380 390 400 410 420 AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ NALLFAEEED GEAGAEDKRS 430 440 450 460 470 480 QEETPGHRRK EAEGTEEGGE EEDDEEMDPQ TIDSLIELST KLHLPADDVV SIIEEVEEKR 490 500 510 520 530 540 KRKKNAPPEP VPPPRAAPAP THVRSPQPPP PAPAPARDEL PDWNEVLPPW DREEDEVYPP 550 560 570 580 590 600 GPYHPFPNYI RPRTLQPPSA LRRRHYHHAL PPSRHYPGRE AQARRAQEEA EAEERRLQEQ 610 EELENYIEHV LLRRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, structural organization analysis and chromosomal assignment of the human gene for neurosecretory protein VGF." Canu N., Possenti R., Ricco A.S., Rocchi M., Levi A. Genomics 45:443-446(1997) [PubMed: 9344675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS.
[5]	"Neuroendocrine regulatory peptide-1 and -2: novel bioactive peptides processed from VGF." Toshinai K., Nakazato M. Cell. Mol. Life Sci. 66:1939-1945(2009) [PubMed: 19194657] [Abstract] Cited for: FUNCTION OF PEPTIDES NERP-1 AND NERP-2, PYROGLUTAMATE FORMATION AT GLN-310, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y12661 Genomic DNA. Translation: CAA73210.1. AC004876 Genomic DNA. Translation: AAD45830.1. CH471197 Genomic DNA. Translation: EAW50201.1. BC063835 mRNA. Translation: AAH63835.1.
IPI	IPI00289501.
RefSeq	NP_003369.2. NM_003378.3.
UniGene	Hs.587325.
3D structure databases
ProteinModelPortal	O15240.
ModBase	Search...
Protein-protein interaction databases
STRING	O15240.
PTM databases
PhosphoSite	O15240.
Proteomic databases
PeptideAtlas	O15240.
PRIDE	O15240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000249330; ENSP00000249330; ENSG00000128564.
GeneID	7425.
KEGG	hsa:7425.
Organism-specific databases
CTD	7425.
GeneCards	GC07M100805.
H-InvDB	HIX0033605.
HGNC	HGNC:12684. VGF.
MIM	602186. gene.
neXtProt	NX_O15240.
PharmGKB	PA37305.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12930.
GeneTree	ENSGT00390000017745.
HOGENOM	HBG126508.
HOVERGEN	HBG006806.
InParanoid	O15240.
OMA	EDKRSQE.
OrthoDB	EOG4ZPDVP.
PhylomeDB	O15240.
Gene expression databases
ArrayExpress	O15240.
Bgee	O15240.
CleanEx	HS_VGF.
Genevestigator	O15240.
Family and domain databases
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

VGF_HUMAN

Accession

Primary (citable) accession number: O15240
Secondary accession number(s): Q9UDW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.