Reviewed, UniProtKB/Swiss-Prot O15234 (CASC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein CASC3
Alternative name(s):
    Cancer susceptibility candidate gene 3 protein
    Metastatic lymph node protein 51
      Short name=Protein MLN 51
    Protein barentsz
      Short name=Btz

Gene names

Name:	CASC3
Synonyms:	MLN51

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport.
Subunit structure	Interacts with STAU in an RNA-dependent manner By similarity. Forms homo-oligomers.
Subcellular location	Cytoplasm › perinuclear region By similarity. Nucleus By similarity. Note: Predominantly found in the perinuclear region By similarity. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner By similarity.
Tissue specificity	Widely expressed. Overexpressed in breast cancers and metastasis, as well as in gastric cancers. Ref.2
Domain	The coiled coil domain may be involved in oligomerization.
Post-translational modification	Phosphorylated. Ref.2 Ref.6 Ref.7

Ontologies

Keywords
Biological process	Transport mRNA transport
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Ref.2 Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF4A3	P38919	2	EBI-299118,EBI-299104
MAGOH	P61326	1	EBI-299118,EBI-299134

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 703

703

Protein CASC3

PRO_0000089324

Regions

Coiled coil

95 – 131

37

Potential

Motif

204 – 210

7

Nuclear localization signal 1 Potential

Motif

254 – 262

9

Nuclear localization signal 2 Potential

Motif

462 – 466

5

Nuclear export signal

Compositional bias

41 – 46

6

Poly-Gly

Compositional bias

392 – 395

4

Poly-Pro

Compositional bias

425 – 428

4

Poly-Pro

Compositional bias

643 – 648

6

Poly-Pro

Compositional bias

692 – 695

4

Poly-Pro

Amino acid modifications

Modified residue

148

1

Phosphoserine Ref.7

Modified residue

363

1

Phosphoserine Ref.6

Modified residue

373

1

Phosphoserine Ref.6

Modified residue

477

1

Phosphoserine Ref.7

Secondary structure

1

.

.

.

.

.

.

.

703

Helix Strand Turn

Details...

Helix

172 – 174

3

Helix

226 – 228

3

Helix

233 – 240

8

Sequences

	Sequence		Length	Mass (Da)
	O15234-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 642A4C01C8DD3BE0	FASTA	703	76,278
	10 20 30 40 50 60 MADRRRQRAS QDTEDEESGA SGSDSGGSPL RGGGSCSGSA GGGGSGSLPS QRGGRTGALH 70 80 90 100 110 120 LRRVESGGAK SAEESECESE DGIEGDAVLS DYESAEDSEG EEGEYSEEEN SKVELKSEAN 130 140 150 160 170 180 DAVNSSTKEE KGEEKPDTKS TVTGERQSGD GQESTEPVEN KVGKKGPKHL DDDEDRKNPA 190 200 210 220 230 240 YIPRKGLFFE HDLRGQTQEE EVRPKGRQRK LWKDEGRWEH DKFREDEQAP KSRQELIALY 250 260 270 280 290 300 GYDIRSAHNP DDIKPRRIRK PRYGSPPQRD PNWNGERLNK SHRHQGLGGT LPPRTFINRN 310 320 330 340 350 360 AAGTGRMSAP RNYSRSGGFK EGRAGFRPVE AGGQHGGRSG ETVKHEISYR SRRLEQTSVR 370 380 390 400 410 420 DPSPEADAPV LGSPEKEEAA SEPPAAAPDA APPPPDRPIE KKSYSRARRT RTKVGDAVKL 430 440 450 460 470 480 AEEVPPPPEG LIPAPPVPET TPTPPTKTGT WEAPVDSSTS GLEQDVAQLN IAEQNWSPGQ 490 500 510 520 530 540 PSFLQPRELR GMPNHIHMGA GPPPQFNRME EMGVQGGRAK RYSSQRQRPV PEPPAPPVHI 550 560 570 580 590 600 SIMEGHYYDP LQFQGPIYTH GDSPAPLPPQ GMLVQPGMNL PHPGLHPHQT PAPLPNPGLY 610 620 630 640 650 660 PPPVSMSPGQ PPPQQLLAPT YFSAPGVMNF GNPSYPYAPG ALPPPPPPHL YPNTQAPSQV 670 680 690 700 YGGVTYYNPA QQQVQPKPSP PRRTPQPVTI KPPPPEVVSR GSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of four novel human genes amplified and overexpressed in breast carcinoma and localized to the q11-q21.3 region of chromosome 17." Tomasetto C.L., Regnier C.H., Moog-Lutz C., Mattei M.-G., Chenard M.-P., Lidereau R., Basset P., Rio M.-C. Genomics 28:367-376(1995) [PubMed: 7490069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary carcinoma.
[2]	"Metastatic lymph node 51, a novel nucleo-cytoplasmic protein overexpressed in breast cancer." Degot S.F., Regnier C.H., Wendling C., Chenard M.-P., Rio M.-C., Tomasetto C.L. Oncogene 21:4422-4434(2002) [PubMed: 12080473] [Abstract] Cited for: SEQUENCE REVISION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION. Tissue: Mammary carcinoma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Duodenum and Skin.
[5]	"Targets of gene amplification and overexpression at 17q in gastric cancer." Varis A., Wolf M., Monni O., Vakkari M.-L., Kokkola A., Moskaluk C., Frierson H.F. Jr., Powell S.M., Knuutila S., Kallioniemi A., El-Rifai W. Cancer Res. 62:2625-2629(2002) [PubMed: 11980659] [Abstract] Cited for: OVEREXPRESSION IN GASTRIC CANCERS.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-363 AND SER-373, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148 AND SER-477, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

X80199 mRNA. Translation: CAC27699.1.
AK292425 mRNA. Translation: BAF85114.1.
BC044656 mRNA. Translation: AAH44656.1.
BC050526 mRNA. Translation: AAH50526.1.

IPI

IPI00289491.

RefSeq

NP_031385.2.

UniGene

Hs.705465

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HYI	X-ray	2.30	D/J	170-246	[»]
2J0Q	X-ray	3.20	I/T	137-286	[»]
2J0S	X-ray	2.21	T	137-286	[»]
2J0U	X-ray	3.00	T	137-250	[»]
3EX7	X-ray	2.30	D/I	138-283	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O15234. 4 interactions.

Protein family/group databases

TCDB

3.A.18.1.1. nuclear mRNA exporter (mRNA-E) family.

PTM databases

PhosphoSite

O15234.

Proteomic databases

PRIDE

O15234.

Genome annotation databases

Ensembl

ENSG00000108349. Homo sapiens. [Contig view]

GeneID

22794.

KEGG

hsa:22794.

Organism-specific databases

GeneCards

GC17P035550.

H-InvDB

HIX0013791.

HGNC

HGNC:17040. CASC3.

MIM

606504. gene.

PharmGKB

PA134948596.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O15234.

HOVERGEN

O15234.

OMA

O15234. KSHRHQG.

Gene expression databases

ArrayExpress

O15234.

Bgee

O15234.

CleanEx

HS_CASC3.

GermOnline

ENSG00000108349. Homo sapiens.

Family and domain databases

InterPro

IPR018545. CASC3/Barentsz_eIF4AIII-bd.
[Graphical view]

Pfam

PF09405. Btz. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

43124.

PMAP-CutDB

O15234.

SOURCE

Search...

Entry information

Entry name

CASC3_HUMAN

Accession

Primary (citable) accession number: O15234
Secondary accession number(s): A8K8R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 14, 2001
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)