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Protein

NF-kappa-B-repressing factor

Gene

NKRF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi296 – 388Add BLAST93

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B-repressing factor
Short name:
NFkB-repressing factor
Alternative name(s):
Protein ITBA4
Transcription factor NRF
Gene namesi
Name:NKRF
Synonyms:ITBA4, NRF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000186416.12.
HGNCiHGNC:19374. NKRF.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55922.
OpenTargetsiENSG00000186416.
PharmGKBiPA134990602.

Chemistry databases

ChEMBLiCHEMBL3163.

Polymorphism and mutation databases

BioMutaiNKRF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968691 – 690NF-kappa-B-repressing factorAdd BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei618PhosphoserineCombined sources1
Cross-linki666Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki674Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO15226.
MaxQBiO15226.
PaxDbiO15226.
PeptideAtlasiO15226.
PRIDEiO15226.

2D gel databases

SWISS-2DPAGEiO15226.

PTM databases

iPTMnetiO15226.
PhosphoSitePlusiO15226.

Expressioni

Tissue specificityi

Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.

Gene expression databases

BgeeiENSG00000186416.
CleanExiHS_NKRF.
ExpressionAtlasiO15226. baseline and differential.
GenevisibleiO15226. HS.

Organism-specific databases

HPAiHPA001476.

Interactioni

Subunit structurei

Interacts with NF-kappa-B. Interacts with XRN2 (PubMed:24462208).1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121002. 50 interactors.
DIPiDIP-34570N.
IntActiO15226. 36 interactors.
MINTiMINT-4728497.
STRINGi9606.ENSP00000442308.

Structurei

3D structure databases

ProteinModelPortaliO15226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 596G-patchPROSITE-ProRule annotationAdd BLAST46
Domaini600 – 664R3HPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 296Active repression domainAdd BLAST296

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi25 – 45Nuclear localization signalAdd BLAST21

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000230930.
HOVERGENiHBG052630.
InParanoidiO15226.
OMAiVCQIGMP.
OrthoDBiEOG091G0HNZ.
PhylomeDBiO15226.
TreeFamiTF326321.

Family and domain databases

CDDicd02640. R3H_NRF. 1 hit.
Gene3Di3.30.1370.50. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_dom.
IPR036867. R3H_dom_sf.
IPR034071. R3H_NRF.
PfamiView protein in Pfam
PF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 2 hits.
SM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiView protein in PROSITE
PS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15226-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK
60 70 80 90 100
FHARPRFEPV HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY
110 120 130 140 150
TQDSFSIQDG NSQYCDSSGF ILTKDQPVTA NMYFDSGNPA PSTTSQQANS
160 170 180 190 200
QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT
210 220 230 240 250
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY
260 270 280 290 300
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI
310 320 330 340 350
GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI
360 370 380 390 400
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA
410 420 430 440 450
AADEALKILQ KTQPTYPSVK SSQCHTGSSP RGSGKKKDIK DLVVYENSSN
460 470 480 490 500
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKTVK
510 520 530 540 550
YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS AEEAYKQQIK
560 570 580 590 600
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
610 620 630 640 650
IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH
660 670 680 690
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
Length:690
Mass (Da):77,673
Last modified:August 15, 2003 - v2
Checksum:i3067A52671A7AE8F
GO
Isoform 2 (identifier: O15226-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGFMLPLIFRYSPRLM

Note: No experimental confirmation available.
Show »
Length:705
Mass (Da):79,496
Checksum:i48B245E4929BD14B
GO

Sequence cautioni

The sequence AL539002 differs from that shown. Probable cloning artifact.Curated
The sequence CAB56459 differs from that shown. Reason: Frameshift at positions 378, 405, 410, 441, 502, 516, 588, 599, 601 and 681.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71R → G in CAB56459 (PubMed:10562553).Curated1
Sequence conflicti453C → G in CAA68976 (PubMed:9224902).Curated1
Sequence conflicti614 – 615AR → ES in CAB56459 (PubMed:10562553).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0473771M → MGFMLPLIFRYSPRLM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011812 mRNA. Translation: CAB56459.1. Frameshift.
AY208891 mRNA. Translation: AAP43025.1.
AC004913 Genomic DNA. No translation available.
CH471161 Genomic DNA. Translation: EAW89859.1.
BC040379 mRNA. Translation: AAH40379.1.
BC047878 mRNA. Translation: AAH47878.1.
AL539002 mRNA. No translation available.
Y07707 mRNA. Translation: CAA68976.1.
CCDSiCCDS35375.1. [O15226-1]
CCDS55486.1. [O15226-2]
RefSeqiNP_001166958.1. NM_001173487.1. [O15226-2]
NP_001166959.1. NM_001173488.1. [O15226-1]
NP_060014.2. NM_017544.3. [O15226-1]
XP_011529667.1. XM_011531365.2. [O15226-1]
UniGeneiHs.437084.

Genome annotation databases

EnsembliENST00000304449; ENSP00000304803; ENSG00000186416. [O15226-1]
ENST00000371527; ENSP00000360582; ENSG00000186416. [O15226-1]
ENST00000542113; ENSP00000442308; ENSG00000186416. [O15226-2]
GeneIDi55922.
KEGGihsa:55922.
UCSCiuc004erq.3. human. [O15226-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNKRF_HUMAN
AccessioniPrimary (citable) accession number: O15226
Secondary accession number(s): G3V1N1, Q4VC41, Q9UJ91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 15, 2003
Last modified: October 25, 2017
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot