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O15226

- NKRF_HUMAN

UniProt

O15226 - NKRF_HUMAN

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Protein
NF-kappa-B-repressing factor
Gene
NKRF, ITBA4, NRF
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi296 – 38893
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: UniProtKB
  3. protein binding Source: IntAct

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: UniProtKB
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B-repressing factor
Short name:
NFkB-repressing factor
Alternative name(s):
Protein ITBA4
Transcription factor NRF
Gene namesi
Name:NKRF
Synonyms:ITBA4, NRF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:19374. NKRF.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134990602.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690NF-kappa-B-repressing factor
PRO_0000096869Add
BLAST

Proteomic databases

MaxQBiO15226.
PaxDbiO15226.
PeptideAtlasiO15226.
PRIDEiO15226.

2D gel databases

SWISS-2DPAGEO15226.

PTM databases

PhosphoSiteiO15226.

Expressioni

Tissue specificityi

Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.

Gene expression databases

ArrayExpressiO15226.
BgeeiO15226.
CleanExiHS_NKRF.
GenevestigatoriO15226.

Interactioni

Subunit structurei

Interacts with NF-kappa-B.

Binary interactionsi

WithEntry#Exp.IntActNotes
DHX58Q96C102EBI-766011,EBI-744193

Protein-protein interaction databases

BioGridi121002. 22 interactions.
DIPiDIP-34570N.
IntActiO15226. 7 interactions.
MINTiMINT-4728497.
STRINGi9606.ENSP00000304803.

Structurei

3D structure databases

ProteinModelPortaliO15226.
SMRiO15226. Positions 348-411, 446-518, 604-663.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini551 – 59646G-patch
Add
BLAST
Domaini600 – 66465R3H
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Active repression domain
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi25 – 4521Nuclear localization signal
Add
BLAST

Sequence similaritiesi

Contains 1 G-patch domain.
Contains 1 R3H domain.

Phylogenomic databases

eggNOGiNOG123689.
HOGENOMiHOG000230930.
HOVERGENiHBG052630.
InParanoidiO15226.
OMAiWRYSHES.
OrthoDBiEOG7XWPN1.
PhylomeDBiO15226.
TreeFamiTF326321.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_ss-bd.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 2 hits.
SM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O15226-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK    50
FHARPRFEPV HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY 100
TQDSFSIQDG NSQYCDSSGF ILTKDQPVTA NMYFDSGNPA PSTTSQQANS 150
QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT 200
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY 250
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI 300
GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI 350
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA 400
AADEALKILQ KTQPTYPSVK SSQCHTGSSP RGSGKKKDIK DLVVYENSSN 450
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKTVK 500
YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS AEEAYKQQIK 550
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK 600
IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH 650
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN 690
Length:690
Mass (Da):77,673
Last modified:August 15, 2003 - v2
Checksum:i3067A52671A7AE8F
GO
Isoform 2 (identifier: O15226-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGFMLPLIFRYSPRLM

Note: No experimental confirmation available.

Show »
Length:705
Mass (Da):79,496
Checksum:i48B245E4929BD14B
GO

Sequence cautioni

The sequence AL539002 differs from that shown. Reason: Probable cloning artifact.
The sequence CAB56459.1 differs from that shown. Reason: Frameshift at positions 378, 405, 410, 441, 502, 516, 588, 599, 601 and 681.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MGFMLPLIFRYSPRLM in isoform 2.
VSP_047377

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711R → G in CAB56459. 1 Publication
Sequence conflicti453 – 4531C → G in CAA68976. 1 Publication
Sequence conflicti614 – 6152AR → ES in CAB56459. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011812 mRNA. Translation: CAB56459.1. Frameshift.
AY208891 mRNA. Translation: AAP43025.1.
AC004913 Genomic DNA. No translation available.
CH471161 Genomic DNA. Translation: EAW89859.1.
BC040379 mRNA. Translation: AAH40379.1.
BC047878 mRNA. Translation: AAH47878.1.
AL539002 mRNA. No translation available.
Y07707 mRNA. Translation: CAA68976.1.
CCDSiCCDS35375.1. [O15226-1]
CCDS55486.1. [O15226-2]
RefSeqiNP_001166958.1. NM_001173487.1. [O15226-2]
NP_001166959.1. NM_001173488.1. [O15226-1]
NP_060014.2. NM_017544.3. [O15226-1]
XP_005262500.1. XM_005262443.2. [O15226-2]
UniGeneiHs.437084.

Genome annotation databases

EnsembliENST00000304449; ENSP00000304803; ENSG00000186416. [O15226-1]
ENST00000371527; ENSP00000360582; ENSG00000186416. [O15226-1]
ENST00000542113; ENSP00000442308; ENSG00000186416. [O15226-2]
GeneIDi55922.
KEGGihsa:55922.
UCSCiuc004erq.3. human. [O15226-1]
uc022cdk.1. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011812 mRNA. Translation: CAB56459.1 . Frameshift.
AY208891 mRNA. Translation: AAP43025.1 .
AC004913 Genomic DNA. No translation available.
CH471161 Genomic DNA. Translation: EAW89859.1 .
BC040379 mRNA. Translation: AAH40379.1 .
BC047878 mRNA. Translation: AAH47878.1 .
AL539002 mRNA. No translation available.
Y07707 mRNA. Translation: CAA68976.1 .
CCDSi CCDS35375.1. [O15226-1 ]
CCDS55486.1. [O15226-2 ]
RefSeqi NP_001166958.1. NM_001173487.1. [O15226-2 ]
NP_001166959.1. NM_001173488.1. [O15226-1 ]
NP_060014.2. NM_017544.3. [O15226-1 ]
XP_005262500.1. XM_005262443.2. [O15226-2 ]
UniGenei Hs.437084.

3D structure databases

ProteinModelPortali O15226.
SMRi O15226. Positions 348-411, 446-518, 604-663.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121002. 22 interactions.
DIPi DIP-34570N.
IntActi O15226. 7 interactions.
MINTi MINT-4728497.
STRINGi 9606.ENSP00000304803.

Chemistry

ChEMBLi CHEMBL3163.

PTM databases

PhosphoSitei O15226.

2D gel databases

SWISS-2DPAGE O15226.

Proteomic databases

MaxQBi O15226.
PaxDbi O15226.
PeptideAtlasi O15226.
PRIDEi O15226.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000304449 ; ENSP00000304803 ; ENSG00000186416 . [O15226-1 ]
ENST00000371527 ; ENSP00000360582 ; ENSG00000186416 . [O15226-1 ]
ENST00000542113 ; ENSP00000442308 ; ENSG00000186416 . [O15226-2 ]
GeneIDi 55922.
KEGGi hsa:55922.
UCSCi uc004erq.3. human. [O15226-1 ]
uc022cdk.1. human.

Organism-specific databases

CTDi 55922.
GeneCardsi GC0XM118722.
HGNCi HGNC:19374. NKRF.
MIMi 300440. gene.
neXtProti NX_O15226.
PharmGKBi PA134990602.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG123689.
HOGENOMi HOG000230930.
HOVERGENi HBG052630.
InParanoidi O15226.
OMAi WRYSHES.
OrthoDBi EOG7XWPN1.
PhylomeDBi O15226.
TreeFami TF326321.

Miscellaneous databases

GeneWikii NKRF_(gene).
GenomeRNAii 55922.
NextBioi 61327.
PROi O15226.
SOURCEi Search...

Gene expression databases

ArrayExpressi O15226.
Bgeei O15226.
CleanExi HS_NKRF.
Genevestigatori O15226.

Family and domain databases

Gene3Di 3.30.1370.50. 1 hit.
3.30.160.20. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_ss-bd.
[Graphical view ]
Pfami PF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 2 hits.
SM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view ]
SUPFAMi SSF82708. SSF82708. 1 hit.
PROSITEi PS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Constitutive silencing of IFN-beta promoter is mediated by NRF (NF-kB-repressing factor), a nuclear inhibitor of NF-kB."
    Nourbakhsh M., Hauser H.
    EMBO J. 18:6415-6425(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Cervix carcinoma.
  2. "Cloning and identification of human transcription factor NRF complete cDNA."
    Mao Y., Xie Y., Dai J.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreas and Skin.
  6. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-258 (ISOFORM 2).
    Tissue: Fetal brain.
  7. "Identification of a new member (ZNF183) of the Ring finger gene family in Xq24-25."
    Frattini A., Faranda S., Bagnasco L., Patrosso C., Nulli P., Zucchi I., Vezzoni P.
    Gene 192:291-298(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 453-690 (ISOFORM 1/2).
    Tissue: Brain.
  8. "Identification of a negative response element in the human inducible nitric-oxide synthase (hiNOS) promoter: the role of NF-kappa B-repressing factor (NRF) in basal repression of the hiNOS gene."
    Feng X., Guo Z., Nourbakhsh M., Hauser H., Ganster R., Shao L., Geller D.A.
    Proc. Natl. Acad. Sci. U.S.A. 99:14212-14217(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNKRF_HUMAN
AccessioniPrimary (citable) accession number: O15226
Secondary accession number(s): G3V1N1, Q4VC41, Q9UJ91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 15, 2003
Last modified: September 3, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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