NF-kappa-B-repressing factor - Homo sapiens (Human)

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O15226 (NKRF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NF-kappa-B-repressing factor
Short name=NFkB-repressing factor

Alternative name(s):
Protein ITBA4
Transcription factor NRF

Gene names

Name:	NKRF
Synonyms:	ITBA4, NRF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. Ref.5
Subunit structure	Interacts with NF-kappa-B.
Subcellular location	Nucleus › nucleolus Ref.6.
Tissue specificity	Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.
Sequence similarities	Contains 1 G-patch domain. Contains 1 R3H domain.
Sequence caution	The sequence CAB56459.1 differs from that shown. Reason: Frameshift at positions 378, 405, 410, 441, 502, 516, 588, 599, 601 and 681.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DHX58	Q96C10	2	EBI-766011,EBI-744193

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 690

690

NF-kappa-B-repressing factor

PRO_0000096869

Regions

Domain

551 – 596

G-patch

Domain

600 – 664

R3H

DNA binding

296 – 388

Region

1 – 296

296

Active repression domain

Motif

25 – 45

Nuclear localization signal

Experimental info

Sequence conflict

R → G in CAB56459. Ref.1

Sequence conflict

453

C → G in CAA68976. Ref.4

Sequence conflict

614 – 615

AR → ES in CAB56459. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O15226 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: 3067A52671A7AE8F
Show »

FASTA

690

77,673

        10         20         30         40         50         60 
MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK FHARPRFEPV 

        70         80         90        100        110        120 
HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY TQDSFSIQDG NSQYCDSSGF 

       130        140        150        160        170        180 
ILTKDQPVTA NMYFDSGNPA PSTTSQQANS QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT 

       190        200        210        220        230        240 
IWKNLSNPEM TSGSDKINYT YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY 

       250        260        270        280        290        300 
ACEVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI 

       310        320        330        340        350        360 
GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI GILNNSASFN 

       370        380        390        400        410        420 
KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA AADEALKILQ KTQPTYPSVK 

       430        440        450        460        470        480 
SSQCHTGSSP RGSGKKKDIK DLVVYENSSN PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC 

       490        500        510        520        530        540 
KVILESEVIA EAVGVKKTVK YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS 

       550        560        570        580        590        600 
AEEAYKQQIK EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK 

       610        620        630        640        650        660 
IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH GVGHDRYLVV 

       670        680        690 
GRKRRKEDLL DQLKQEGQVG HYELVMPQAN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Constitutive silencing of IFN-beta promoter is mediated by NRF (NF-kB-repressing factor), a nuclear inhibitor of NF-kB." Nourbakhsh M., Hauser H. EMBO J. 18:6415-6425(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cervix carcinoma.
[2]	"Cloning and identification of human transcription factor NRF complete cDNA." Mao Y., Xie Y., Dai J. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Skin.
[4]	"Identification of a new member (ZNF183) of the Ring finger gene family in Xq24-25." Frattini A., Faranda S., Bagnasco L., Patrosso C., Nulli P., Zucchi I., Vezzoni P. Gene 192:291-298(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 453-690. Tissue: Brain.
[5]	"Identification of a negative response element in the human inducible nitric-oxide synthase (hiNOS) promoter: the role of NF-kappa B-repressing factor (NRF) in basal repression of the hiNOS gene." Feng X., Guo Z., Nourbakhsh M., Hauser H., Ganster R., Shao L., Geller D.A. Proc. Natl. Acad. Sci. U.S.A. 99:14212-14217(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ011812 mRNA. Translation: CAB56459.1. Frameshift. AY208891 mRNA. Translation: AAP43025.1. BC040379 mRNA. Translation: AAH40379.1. BC047878 mRNA. Translation: AAH47878.1. Y07707 mRNA. Translation: CAA68976.1.
IPI	IPI00005675.
RefSeq	NP_001166958.1. NM_001173487.1. NP_001166959.1. NM_001173488.1. NP_060014.2. NM_017544.3.
UniGene	Hs.437084.
3D structure databases
ProteinModelPortal	O15226.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-34570N.
IntAct	O15226. 5 interactions.
STRING	9606.ENSP00000304803.
PTM databases
PhosphoSite	O15226.
2D gel databases
SWISS-2DPAGE	O15226.
Proteomic databases
PaxDb	O15226.
PeptideAtlas	O15226.
PRIDE	O15226.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000304449; ENSP00000304803; ENSG00000186416. ENST00000371527; ENSP00000360582; ENSG00000186416.
GeneID	55922.
KEGG	hsa:55922.
UCSC	uc004erq.3. human.
Organism-specific databases
CTD	55922.
GeneCards	GC0XM118722.
HGNC	HGNC:19374. NKRF.
MIM	300440. gene.
neXtProt	NX_O15226.
PharmGKB	PA134990602.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG123689.
HOGENOM	HOG000230930.
HOVERGEN	HBG052630.
InParanoid	O15226.
OrthoDB	EOG4M65H5.
PhylomeDB	O15226.
Gene expression databases
ArrayExpress	O15226.
Bgee	O15226.
CleanEx	HS_NKRF.
Genevestigator	O15226.
GermOnline	ENSG00000186416. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR000467. G_patch_dom. IPR001374. R3H_ss-bd. [Graphical view]
Pfam	PF01585. G-patch. 1 hit. PF01424. R3H. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 2 hits. SM00443. G_patch. 1 hit. SM00393. R3H. 1 hit. [Graphical view]
PROSITE	PS50174. G_PATCH. 1 hit. PS51061. R3H. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL3163.
GenomeRNAi	55922.
NextBio	61327.
SOURCE	Search...

Entry information

Entry name

NKRF_HUMAN

Accession

Primary (citable) accession number: O15226
Secondary accession number(s): Q4VC41, Q9UJ91

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 15, 2003
Last modified:	May 1, 2013
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome X: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents