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Protein

Glutathione S-transferase A4

Gene

GSTA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1
Binding sitei212SubstrateCurated1

GO - Molecular functioni

  • glutathione transferase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • glutathione derivative biosynthetic process Source: Reactome
  • glutathione metabolic process Source: UniProtKB
  • xenobiotic metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciZFISH:HS10203-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A4 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 4
Glutathione S-transferase A4-4
Gene namesi
Name:GSTA4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:4629. GSTA4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9Y → F: Reduces catalytic activity 70-fold. 1 Publication1
Mutagenesisi212Y → A, F or S: Strongly reduced activity towards 4-hydroxynon-2-enal and 1-chloro-2,4-dinitrobenzene. 1 Publication1

Organism-specific databases

DisGeNETi2941.
OpenTargetsiENSG00000170899.
PharmGKBiPA29019.

Chemistry databases

ChEMBLiCHEMBL4933.
DrugBankiDB00143. Glutathione.

Polymorphism and mutation databases

BioMutaiGSTA4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857861 – 222Glutathione S-transferase A4Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO15217.
PaxDbiO15217.
PeptideAtlasiO15217.
PRIDEiO15217.

PTM databases

iPTMnetiO15217.
PhosphoSitePlusiO15217.

Expressioni

Tissue specificityi

Expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver.

Gene expression databases

BgeeiENSG00000170899.
CleanExiHS_GSTA4.
ExpressionAtlasiO15217. baseline and differential.
GenevisibleiO15217. HS.

Organism-specific databases

HPAiHPA048934.

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-752440,EBI-752440
C2orf50Q96LR75EBI-752440,EBI-10290932
GSTA2P092105EBI-752440,EBI-10196201

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi109196. 7 interactors.
IntActiO15217. 8 interactors.
MINTiMINT-1466182.
STRINGi9606.ENSP00000359998.

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni14 – 16Combined sources3
Helixi17 – 25Combined sources9
Beta strandi31 – 34Combined sources4
Helixi38 – 46Combined sources9
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Helixi86 – 108Combined sources23
Helixi109 – 111Combined sources3
Helixi114 – 131Combined sources18
Helixi133 – 142Combined sources10
Beta strandi146 – 149Combined sources4
Helixi155 – 170Combined sources16
Turni172 – 177Combined sources6
Helixi179 – 189Combined sources11
Helixi192 – 198Combined sources7
Beta strandi199 – 203Combined sources5
Helixi210 – 220Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GULX-ray2.70A/B/C/D/E/F/G/H1-222[»]
1GUMX-ray3.00A/B/C/D/E/F/G/H1-222[»]
3IK7X-ray1.97A/B/C/D1-222[»]
ProteinModelPortaliO15217.
SMRiO15217.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15217.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 208GST C-terminalAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiO15217.
KOiK00799.
OMAiEMIIMHP.
OrthoDBiEOG091G0O3D.
PhylomeDBiO15217.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15217-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARPKLHYP NGRGRMESVR WVLAAAGVEF DEEFLETKEQ LYKLQDGNHL
60 70 80 90 100
LFQQVPMVEI DGMKLVQTRS ILHYIADKHN LFGKNLKERT LIDMYVEGTL
110 120 130 140 150
DLLELLIMHP FLKPDDQQKE VVNMAQKAII RYFPVFEKIL RGHGQSFLVG
160 170 180 190 200
NQLSLADVIL LQTILALEEK IPNILSAFPF LQEYTVKLSN IPTIKRFLEP
210 220
GSKKKPPPDE IYVRTVYNIF RP
Length:222
Mass (Da):25,704
Last modified:January 1, 1998 - v1
Checksum:i1BD98B41E413BB4E
GO
Isoform 2 (identifier: O15217-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Show »
Length:129
Mass (Da):14,884
Checksum:i746CA2AA5C2BCA24
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022210100L → P.1 PublicationCorresponds to variant rs45551133dbSNPEnsembl.1
Natural variantiVAR_022211163T → A.1 PublicationCorresponds to variant rs4147617dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564731 – 93Missing in isoform 2. 2 PublicationsAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13047 mRNA. Translation: CAA73482.1.
AF020918 mRNA. Translation: AAD04711.1.
AF025887 mRNA. Translation: AAC39695.1.
AF125271 mRNA. Translation: AAD27704.1.
AF125272 mRNA. Translation: AAD27705.1.
AF125273 mRNA. Translation: AAD27706.1.
AF050059
, AF050054, AF050055, AF050056, AF050057, AF050058 Genomic DNA. Translation: AAC72706.1.
AY878121 Genomic DNA. Translation: AAW56075.1.
AK315369 mRNA. Translation: BAG37762.1.
CR749474 mRNA. Translation: CAH18304.1.
AL121969, AL162581 Genomic DNA. Translation: CAI42451.1.
AL162581, AL121969 Genomic DNA. Translation: CAI19517.1.
CH471081 Genomic DNA. Translation: EAX04393.1.
CH471081 Genomic DNA. Translation: EAX04394.1.
CH471081 Genomic DNA. Translation: EAX04395.1.
CH471081 Genomic DNA. Translation: EAX04396.1.
CH471081 Genomic DNA. Translation: EAX04397.1.
BC015523 mRNA. Translation: AAH15523.1.
BC063439 mRNA. Translation: AAH63439.1.
CCDSiCCDS4948.1. [O15217-1]
RefSeqiNP_001503.1. NM_001512.3. [O15217-1]
XP_005249092.1. XM_005249035.3. [O15217-1]
UniGeneiHs.485557.

Genome annotation databases

EnsembliENST00000370959; ENSP00000359998; ENSG00000170899. [O15217-1]
ENST00000370960; ENSP00000359999; ENSG00000170899. [O15217-2]
ENST00000370963; ENSP00000360002; ENSG00000170899. [O15217-1]
GeneIDi2941.
KEGGihsa:2941.
UCSCiuc003pbf.4. human. [O15217-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13047 mRNA. Translation: CAA73482.1.
AF020918 mRNA. Translation: AAD04711.1.
AF025887 mRNA. Translation: AAC39695.1.
AF125271 mRNA. Translation: AAD27704.1.
AF125272 mRNA. Translation: AAD27705.1.
AF125273 mRNA. Translation: AAD27706.1.
AF050059
, AF050054, AF050055, AF050056, AF050057, AF050058 Genomic DNA. Translation: AAC72706.1.
AY878121 Genomic DNA. Translation: AAW56075.1.
AK315369 mRNA. Translation: BAG37762.1.
CR749474 mRNA. Translation: CAH18304.1.
AL121969, AL162581 Genomic DNA. Translation: CAI42451.1.
AL162581, AL121969 Genomic DNA. Translation: CAI19517.1.
CH471081 Genomic DNA. Translation: EAX04393.1.
CH471081 Genomic DNA. Translation: EAX04394.1.
CH471081 Genomic DNA. Translation: EAX04395.1.
CH471081 Genomic DNA. Translation: EAX04396.1.
CH471081 Genomic DNA. Translation: EAX04397.1.
BC015523 mRNA. Translation: AAH15523.1.
BC063439 mRNA. Translation: AAH63439.1.
CCDSiCCDS4948.1. [O15217-1]
RefSeqiNP_001503.1. NM_001512.3. [O15217-1]
XP_005249092.1. XM_005249035.3. [O15217-1]
UniGeneiHs.485557.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GULX-ray2.70A/B/C/D/E/F/G/H1-222[»]
1GUMX-ray3.00A/B/C/D/E/F/G/H1-222[»]
3IK7X-ray1.97A/B/C/D1-222[»]
ProteinModelPortaliO15217.
SMRiO15217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109196. 7 interactors.
IntActiO15217. 8 interactors.
MINTiMINT-1466182.
STRINGi9606.ENSP00000359998.

Chemistry databases

ChEMBLiCHEMBL4933.
DrugBankiDB00143. Glutathione.

PTM databases

iPTMnetiO15217.
PhosphoSitePlusiO15217.

Polymorphism and mutation databases

BioMutaiGSTA4.

Proteomic databases

MaxQBiO15217.
PaxDbiO15217.
PeptideAtlasiO15217.
PRIDEiO15217.

Protocols and materials databases

DNASUi2941.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370959; ENSP00000359998; ENSG00000170899. [O15217-1]
ENST00000370960; ENSP00000359999; ENSG00000170899. [O15217-2]
ENST00000370963; ENSP00000360002; ENSG00000170899. [O15217-1]
GeneIDi2941.
KEGGihsa:2941.
UCSCiuc003pbf.4. human. [O15217-1]

Organism-specific databases

CTDi2941.
DisGeNETi2941.
GeneCardsiGSTA4.
HGNCiHGNC:4629. GSTA4.
HPAiHPA048934.
MIMi605450. gene.
neXtProtiNX_O15217.
OpenTargetsiENSG00000170899.
PharmGKBiPA29019.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiO15217.
KOiK00799.
OMAiEMIIMHP.
OrthoDBiEOG091G0O3D.
PhylomeDBiO15217.
TreeFamiTF105321.

Enzyme and pathway databases

BioCyciZFISH:HS10203-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.

Miscellaneous databases

ChiTaRSiGSTA4. human.
EvolutionaryTraceiO15217.
GeneWikiiGSTA4.
GenomeRNAii2941.
PROiO15217.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170899.
CleanExiHS_GSTA4.
ExpressionAtlasiO15217. baseline and differential.
GenevisibleiO15217. HS.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA4_HUMAN
AccessioniPrimary (citable) accession number: O15217
Secondary accession number(s): B2RD15
, Q5T7Q8, Q6P4G1, Q9BX18, Q9H414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.