O15211 (RGL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ral guanine nucleotide dissociation stimulator-like 2
Short name=RalGDS-like 2

Alternative name(s):
RalGDS-like factor
Ras-associated protein RAB2L

Gene names

Name:	RGL2
Synonyms:	RAB2L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro By similarity.
Subunit structure	Interacts with SAMD9. Ref.9
Sequence similarities	Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-associating domain. Contains 1 Ras-GEF domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Non-traceable author statement Ref.6. Source: UniProtKB regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	Ras guanyl-nucleotide exchange factor activity Non-traceable author statement Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	Ral guanine nucleotide dissociation stimulator-like 2		PRO_0000068888
Regions
	Domain	88 – 212	125	N-terminal Ras-GEF
	Domain	243 – 513	271	Ras-GEF
	Domain	648 – 735	88	Ras-associating
Amino acid modifications
	Modified residue	617	1	Phosphoserine By similarity
Natural variations
	Natural variant	598	1	P → L. Corresponds to variant rs34022110 [ dbSNP | Ensembl ].		VAR_051903
	Natural variant	705	1	G → E. Corresponds to variant rs35273540 [ dbSNP | Ensembl ].		VAR_051904
Experimental info
	Sequence conflict	720	1	A → P in BAA75926. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O15211 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 91AFBAB4D6CEE4A1	FASTA	777	83,549
	10 20 30 40 50 60 MLPRPLRLLL DTSPPGGVVL SSFRSRDPEE GGGPGGLVVG GGQEEEEEEE EEAPVSVWDE 70 80 90 100 110 120 EEDGAVFTVT SRQYRPLDPL VPMPPPRSSR RLRAGTLEAL VRHLLDTRTS GTDVSFMSAF 130 140 150 160 170 180 LATHRAFTST PALLGLMADR LEALESHPTD ELERTTEVAI SVLSTWLASH PEDFGSEAKG 190 200 210 220 230 240 QLDRLESFLL QTGYAAGKGV GGGSADLIRN LRSRVDPQAP DLPKPLALPG DPPADPTDVL 250 260 270 280 290 300 VFLADHLAEQ LTLLDAELFL NLIPSQCLGG LWGHRDRPGH SHLCPSVRAT VTQFNKVAGA 310 320 330 340 350 360 VVSSVLGATS TGEGPGEVTI RPLRPPQRAR LLEKWIRVAE ECRLLRNFSS VYAVVSALQS 370 380 390 400 410 420 SPIHRLRAAW GEATRDSLRV FSSLCQIFSE EDNYSQSREL LVQEVKLQSP LEPHSKKAPR 430 440 450 460 470 480 SGSRGGGVVP YLGTFLKDLV MLDAASKDEL ENGYINFDKR RKEFAVLSEL RRLQNECRGY 490 500 510 520 530 540 NLQPDHDIQR WLQGLRPLTE AQSHRVSCEV EPPGSSDPPA PRVLRPTLVI SQWTEVLGSV 550 560 570 580 590 600 GVPTPLVSCD RPSTGGDEAP TTPAPLLTRL AQHMKWPSVS SLDSALESSP SLHSPADPSH 610 620 630 640 650 660 LSPPASSPRP SRGHRRSASC GSPLSGGAEE ASGGTGYGGE GSGPGASDCR IIRVQMELGE 670 680 690 700 710 720 DGSVYKSILV TSQDKAPSVI SRVLKKNNRD SAVASEYELV QLLPGERELT IPASANVFYA 730 740 750 760 770 MDGASHDFLL RQRRRSSTAT PGVTSGPSAS GTPPSEGGGG SFPRIKATGR KIARALF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TAPASIN, DAXX, RGL2, HKE2 and four new genes (BING 1, 3 to 5) form a dense cluster at the centromeric end of the MHC." Herberg J.A., Beck S., Trowsdale J. J. Mol. Biol. 277:839-857(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	"Isolation and mapping of RAB2L, a human cDNA that encodes a protein homologous to RalGDS." Isomura M., Okui K., Fujiwara T., Shin S., Nakamura Y. Cytogenet. Cell Genet. 74:263-265(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 656-777.
[7]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Functional characterization of SAMD9, a protein deficient in normophosphatemic familial tumoral calcinosis." Hershkovitz D., Gross Y., Nahum S., Yehezkel S., Sarig O., Uitto J., Sprecher E. J. Invest. Dermatol. 131:662-669(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SAMD9.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z97184 Genomic DNA. Translation: CAB09992.1. AL050259 mRNA. Translation: CAB43361.1. AL662827 Genomic DNA. Translation: CAI17521.1. AL662820 Genomic DNA. Translation: CAI18118.1. BX000343 Genomic DNA. Translation: CAI41832.1. CR759817 Genomic DNA. Translation: CAQ08027.1. CR759786 Genomic DNA. Translation: CAQ08257.1. CH471081 Genomic DNA. Translation: EAX03709.1. BC032681 mRNA. Translation: AAH32681.1. D85757 mRNA. Translation: BAA75926.1.
IPI	IPI00005656.
PIR	T08659.
RefSeq	NP_001230667.1. NM_001243738.1. NP_004752.1. NM_004761.4.
UniGene	Hs.509622.
3D structure databases
ProteinModelPortal	O15211.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15211. 13 interactions.
STRING	9606.ENSP00000407088.
PTM databases
PhosphoSite	O15211.
Proteomic databases
PaxDb	O15211.
PRIDE	O15211.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000383204; ENSP00000372691; ENSG00000206282. ENST00000413136; ENSP00000407088; ENSG00000224841. ENST00000416548; ENSP00000412152; ENSG00000228736. ENST00000452084; ENSP00000390098; ENSG00000237825. ENST00000497454; ENSP00000420211; ENSG00000237441.
GeneID	5863.
KEGG	hsa:5863.
UCSC	uc003odv.3. human.
Organism-specific databases
CTD	5863.
GeneCards	GC06M033259.
HGNC	HGNC:9769. RGL2.
HPA	HPA053444.
MIM	602306. gene.
neXtProt	NX_O15211.
PharmGKB	PA34120.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG298772.
HOGENOM	HOG000231592.
HOVERGEN	HBG005864.
InParanoid	O15211.
OMA	NLVPSQC.
OrthoDB	EOG4N30NR.
PhylomeDB	O15211.
Gene expression databases
ArrayExpress	O15211.
Bgee	O15211.
CleanEx	HS_RGL2.
Genevestigator	O15211.
GermOnline	ENSG00000204218. Homo sapiens.
Family and domain databases
Gene3D	1.10.840.10. 1 hit.
InterPro	IPR000159. Ras-assoc. IPR000651. Ras-like_Gua-exchang_fac_N. IPR019804. Ras_G-nucl-exch_fac_CS. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00788. RA. 1 hit. PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. [Graphical view]
SMART	SM00314. RA. 1 hit. SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit.
PROSITE	PS50200. RA. 1 hit. PS00720. RASGEF. 1 hit. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5863.
NextBio	22770.
SOURCE	Search...

Entry information

Entry name

RGL2_HUMAN

Accession

Primary (citable) accession number: O15211
Secondary accession number(s): Q5STK0, Q9Y3F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.