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O15194

- CTDSL_HUMAN

UniProt

O15194 - CTDSL_HUMAN

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Protein

CTD small phosphatase-like protein

Gene

CTDSPL

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei112 – 11214-aspartylphosphate intermediateBy similarity
Metal bindingi112 – 1121MagnesiumBy similarity
Active sitei114 – 1141Proton donorBy similarity
Metal bindingi114 – 1141Magnesium; via carbonyl oxygenBy similarity
Sitei168 – 1681Transition state stabilizerBy similarity
Sitei206 – 2061Transition state stabilizerBy similarity
Metal bindingi223 – 2231MagnesiumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CTD small phosphatase-like protein (EC:3.1.3.16)
Short name:
CTDSP-like
Alternative name(s):
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
NIF-like protein
Nuclear LIM interactor-interacting factor 1
Short name:
NLI-interacting factor 1
Protein YA22
Short name:
hYA22
RBSP3
Small C-terminal domain phosphatase 3
Short name:
SCP3
Short name:
Small CTD phosphatase 3
Gene namesi
Name:CTDSPL
Synonyms:C3orf8, NIF1, NIFL, SCP3, YA22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:16890. CTDSPL.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394571.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276CTD small phosphatase-like proteinPRO_0000212569Add
BLAST

Proteomic databases

MaxQBiO15194.
PaxDbiO15194.
PRIDEiO15194.

PTM databases

PhosphoSiteiO15194.

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues.1 Publication

Gene expression databases

BgeeiO15194.
CleanExiHS_CTDSPL.
ExpressionAtlasiO15194. baseline and differential.
GenevestigatoriO15194.

Organism-specific databases

HPAiHPA048325.

Interactioni

Subunit structurei

Interacts with REST (By similarity). Monomer.By similarity1 Publication

Protein-protein interaction databases

BioGridi115512. 7 interactions.
STRINGi9606.ENSP00000273179.

Structurei

Secondary structure

1
276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi93 – 964Combined sources
Helixi101 – 1033Combined sources
Beta strandi108 – 1114Combined sources
Turni115 – 1173Combined sources
Beta strandi118 – 1236Combined sources
Beta strandi129 – 1368Combined sources
Beta strandi139 – 1479Combined sources
Helixi151 – 16111Combined sources
Beta strandi162 – 1676Combined sources
Helixi172 – 18211Combined sources
Beta strandi188 – 1925Combined sources
Helixi194 – 1963Combined sources
Beta strandi198 – 2003Combined sources
Beta strandi203 – 2053Combined sources
Helixi208 – 2103Combined sources
Beta strandi211 – 2133Combined sources
Helixi215 – 2173Combined sources
Beta strandi218 – 2236Combined sources
Helixi225 – 2284Combined sources
Helixi232 – 2343Combined sources
Beta strandi235 – 2373Combined sources
Helixi249 – 26113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HHLX-ray2.10A/B/C/D82-265[»]
ProteinModelPortaliO15194.
SMRiO15194. Positions 82-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15194.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 260159FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00390000017194.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiO15194.
KOiK15731.
OMAiHMSIITQ.
OrthoDBiEOG71P2C3.
PhylomeDBiO15194.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O15194-1) [UniParc]FASTAAdd to Basket

Also known as: HYA22B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGPAIITQV TNPKEDEGRL PGAGEKASQC NVSLKKQRSR SILSSFFCCF
60 70 80 90 100
RDYNVEAPPP SSPSVLPPLV EENGGLQKGD QRQVIPIPSP PAKYLLPEVT
110 120 130 140 150
VLDYGKKCVV IDLDETLVHS SFKPISNADF IVPVEIDGTI HQVYVLKRPH
160 170 180 190 200
VDEFLQRMGQ LFECVLFTAS LAKYADPVAD LLDRWGVFRA RLFRESCVFH
210 220 230 240 250
RGNYVKDLSR LGRELSKVII VDNSPASYIF HPENAVPVQS WFDDMTDTEL
260 270
LDLIPFFEGL SREDDVYSML HRLCNR
Length:276
Mass (Da):31,129
Last modified:August 31, 2004 - v2
Checksum:i5C7B32A1127B0F59
GO
Isoform 2 (identifier: O15194-2) [UniParc]FASTAAdd to Basket

Also known as: HYA22A

The sequence of this isoform differs from the canonical sequence as follows:
     79-89: Missing.

Show »
Length:265
Mass (Da):29,937
Checksum:iD19C4461386C1C2F
GO

Sequence cautioni

The sequence BAA21667.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti121 – 1211S → P.1 Publication
VAR_019683
Natural varianti127 – 1271N → S.1 Publication
VAR_019684
Natural varianti132 – 1321V → G.1 Publication
VAR_019685

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 8911Missing in isoform 2. 4 PublicationsVSP_011541Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88153 mRNA. Translation: BAA21667.1. Different initiation.
AY279532 mRNA. Translation: AAP34400.1.
AJ575644 mRNA. Translation: CAE11804.1.
AJ575645 mRNA. Translation: CAE11805.1.
AY364238 mRNA. Translation: AAQ76797.1.
CCDSiCCDS33734.1. [O15194-1]
CCDS33735.1. [O15194-2]
PIRiJC5707.
RefSeqiNP_001008393.1. NM_001008392.1. [O15194-1]
NP_005799.2. NM_005808.2. [O15194-2]
UniGeneiHs.475963.

Genome annotation databases

EnsembliENST00000273179; ENSP00000273179; ENSG00000144677. [O15194-1]
ENST00000443503; ENSP00000398288; ENSG00000144677. [O15194-2]
GeneIDi10217.
KEGGihsa:10217.
UCSCiuc003chg.3. human. [O15194-1]
uc003chh.3. human. [O15194-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88153 mRNA. Translation: BAA21667.1 . Different initiation.
AY279532 mRNA. Translation: AAP34400.1 .
AJ575644 mRNA. Translation: CAE11804.1 .
AJ575645 mRNA. Translation: CAE11805.1 .
AY364238 mRNA. Translation: AAQ76797.1 .
CCDSi CCDS33734.1. [O15194-1 ]
CCDS33735.1. [O15194-2 ]
PIRi JC5707.
RefSeqi NP_001008393.1. NM_001008392.1. [O15194-1 ]
NP_005799.2. NM_005808.2. [O15194-2 ]
UniGenei Hs.475963.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HHL X-ray 2.10 A/B/C/D 82-265 [» ]
ProteinModelPortali O15194.
SMRi O15194. Positions 82-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115512. 7 interactions.
STRINGi 9606.ENSP00000273179.

PTM databases

PhosphoSitei O15194.

Proteomic databases

MaxQBi O15194.
PaxDbi O15194.
PRIDEi O15194.

Protocols and materials databases

DNASUi 10217.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000273179 ; ENSP00000273179 ; ENSG00000144677 . [O15194-1 ]
ENST00000443503 ; ENSP00000398288 ; ENSG00000144677 . [O15194-2 ]
GeneIDi 10217.
KEGGi hsa:10217.
UCSCi uc003chg.3. human. [O15194-1 ]
uc003chh.3. human. [O15194-2 ]

Organism-specific databases

CTDi 10217.
GeneCardsi GC03P037878.
HGNCi HGNC:16890. CTDSPL.
HPAi HPA048325.
MIMi 608592. gene.
neXtProti NX_O15194.
PharmGKBi PA128394571.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5190.
GeneTreei ENSGT00390000017194.
HOGENOMi HOG000236379.
HOVERGENi HBG053298.
InParanoidi O15194.
KOi K15731.
OMAi HMSIITQ.
OrthoDBi EOG71P2C3.
PhylomeDBi O15194.
TreeFami TF313556.

Miscellaneous databases

ChiTaRSi CTDSPL. human.
EvolutionaryTracei O15194.
GeneWikii CTDSPL.
GenomeRNAii 10217.
NextBioi 38686.
PROi O15194.
SOURCEi Search...

Gene expression databases

Bgeei O15194.
CleanExi HS_CTDSPL.
ExpressionAtlasi O15194. baseline and differential.
Genevestigatori O15194.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view ]
Pfami PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00577. CPDc. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR02251. HIF-SF_euk. 1 hit.
PROSITEi PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 685-kb genomic region on chromosome 3p22-p21.3 that is homozygously deleted in a lung carcinoma cell line."
    Ishikawa S., Kai M., Tamari M., Takei Y., Takeuchi K., Bandou H., Yamane Y., Ogawa M., Nakamura Y.
    DNA Res. 4:35-43(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Fetal brain, Lung and Pancreas.
  2. "A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5."
    Yeo M., Lin P.S., Dahmus M.E., Gill G.N.
    J. Biol. Chem. 278:26078-26085(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS PRO-121; SER-127 AND GLY-132.
    Tissue: Lung.
  4. "NovelFam3000 -- uncharacterized human protein domains conserved across model organisms."
    Kemmer D., Podowski R.M., Arenillas D., Lim J., Hodges E., Roth P., Sonnhammer E.L.L., Hoeoeg C., Wasserman W.W.
    BMC Genomics 7:48-48(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "Small CTD phosphatases function in silencing neuronal gene expression."
    Yeo M., Lee S.-K., Lee B., Ruiz E.C., Pfaff S.L., Gill G.N.
    Science 307:596-600(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 82-265, SUBUNIT.

Entry informationi

Entry nameiCTDSL_HUMAN
AccessioniPrimary (citable) accession number: O15194
Secondary accession number(s): Q3ZTU0, Q70KI4, Q7Z5Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: August 31, 2004
Last modified: November 26, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3