O15156 (ZBT7B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger and BTB domain-containing protein 7B

Alternative name(s):
Krueppel-related zinc finger protein cKrox
Short name=hcKrox
T-helper-inducing POZ/Krueppel-like factor
Zinc finger and BTB domain-containing protein 15
Zinc finger protein 67 homolog
Short name=Zfp-67
Zinc finger protein 857B
Zinc finger protein Th-POK

Gene names

Name:	ZBTB7B
Synonyms:	ZBTB15, ZFP67, ZNF857B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes By similarity. Ref.1
Subcellular location	Nucleus.
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 4 C2H2-type zinc fingers.
Sequence caution	The sequence CAH18158.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW ectoderm development Traceable author statement. Source: ProtInc multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from direct assay. Source: HPA
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Zinc finger and BTB domain-containing protein 7B		PRO_0000047719
Regions
	Domain	34 – 115	82	BTB
	Zinc finger	346 – 368	23	C2H2-type 1
	Zinc finger	374 – 396	23	C2H2-type 2
	Zinc finger	402 – 424	23	C2H2-type 3
	Zinc finger	430 – 454	25	C2H2-type 4; atypical
	Compositional bias	181 – 196	16	Pro-rich
Amino acid modifications
	Modified residue	369	1	Phosphothreonine Ref.6
Experimental info
	Sequence conflict	437	1	K → R in AAC51847. Ref.1
	Sequence conflict	480	1	S → F in AAC51847. Ref.1
	Sequence conflict	509 – 510	2	GP → WA in AAC51847. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O15156 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 86B3616504B05B7E	FASTA	539	58,027
	10 20 30 40 50 60 MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA VLAACSHYFK 70 80 90 100 110 120 KLFTEGGGGA VMGAGGSGTA TGGAGAGVCE LDFVGPEALG ALLEFAYTAT LTTSSANMPA 130 140 150 160 170 180 VLQAARLLEI PCVIAACMEI LQGSGLEAPS PDEDDCERAR QYLEAFATAT ASGVPNGEDS 190 200 210 220 230 240 PPQVPLPPPP PPPPRPVARR SRKPRKAFLQ TKGARANHLV PEVPTVPAHP LTYEEEEVAG 250 260 270 280 290 300 RVGSSGGSGP GDSYSPPTGT ASPPEGPQSY EPYEGEEEEE ELVYPPAYGL AQGGGPPLSP 310 320 330 340 350 360 EELGSDEDAI DPDLMAYLSS LHQDNLAPGL DSQDKLVRKR RSQMPQECPV CHKIIHGAGK 370 380 390 400 410 420 LPRHMRTHTG EKPFACEVCG VRFTRNDKLK IHMRKHTGER PYSCPHCPAR FLHSYDLKNH 430 440 450 460 470 480 MHLHTGDRPY ECHLCHKAFA KEDHLQRHLK GQNCLEVRTR RRRKDDAPPH YPPPSTAAAS 490 500 510 520 530 PAGLDLSNGH LDTFRLSLAR FWEQSAPTGP PVSTPGPPDD DEEEGAPTTP QAEGAMESS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of hcKrox, a transcriptional regulator of extracellular matrix gene expression." Widom R.L., Culic I., Lee J.Y., Korn J.H. Gene 198:407-420(1997) [PubMed: 9370309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Skin fibroblast.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Salivary gland.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-369, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF007833 mRNA. Translation: AAC51847.1. CR749303 mRNA. Translation: CAH18158.1. Different initiation. AL451085 Genomic DNA. Translation: CAI13266.1. CH471121 Genomic DNA. Translation: EAW53149.1. CH471121 Genomic DNA. Translation: EAW53150.1. CH471121 Genomic DNA. Translation: EAW53151.1. BC012070 mRNA. Translation: AAH12070.1.
IPI	IPI00026277.
RefSeq	NP_001239334.1. NM_001252405.1. NP_056956.2. NM_015872.3.
UniGene	Hs.729279.
3D structure databases
ProteinModelPortal	O15156.
SMR	O15156. Positions 7-143, 332-454.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15156. 2 interactions.
MINT	MINT-1451723.
STRING	O15156.
PTM databases
PhosphoSite	O15156.
Proteomic databases
PRIDE	O15156.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000292176; ENSP00000292176; ENSG00000160685. ENST00000368426; ENSP00000357411; ENSG00000160685. ENST00000417934; ENSP00000406286; ENSG00000160685.
GeneID	51043.
KEGG	hsa:51043.
UCSC	uc001fgj.2. human.
Organism-specific databases
CTD	51043.
GeneCards	GC01P154975.
H-InvDB	HIX0028581.
HGNC	HGNC:18668. ZBTB7B.
HPA	HPA006811. HPA025820.
MIM	607646. gene.
neXtProt	NX_O15156.
PharmGKB	PA38630.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14380.
GeneTree	ENSGT00600000084004.
HOVERGEN	HBG059461.
InParanoid	O15156.
OrthoDB	EOG47PX6M.
PhylomeDB	O15156.
Gene expression databases
ArrayExpress	O15156.
Bgee	O15156.
CleanEx	HS_ZBTB7B.
Genevestigator	O15156.
GermOnline	ENSG00000160685. Homo sapiens.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit. G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 4 hits.
KO	K10494.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 3 hits. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 4 hits. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	53600.
SOURCE	Search...

Entry information

Entry name

ZBT7B_HUMAN

Accession

Primary (citable) accession number: O15156
Secondary accession number(s): D3DV83, Q68DR2, Q96EP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.