O15155 (BET1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
BET1 homolog
Short name=hBET1

Alternative name(s):
Golgi vesicular membrane-trafficking protein p18

Gene names

Name:

BET1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the BET1 family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Traceable author statement PubMed 8621431. Source: ProtInc protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle fusion with Golgi apparatus Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi cisterna Inferred from electronic annotation. Source: Compara Golgi membrane Traceable author statement PubMed 8621431. Source: ProtInc cis-Golgi network Inferred from electronic annotation. Source: Compara endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
USHBP1	Q8N6Y0	2	EBI-749204,EBI-739895

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	BET1 homolog		PRO_0000206884
Regions
	Topological domain	1 – 94	94	Cytoplasmic Potential
	Transmembrane	95 – 115	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	116 – 118	3	Vesicular Potential
	Domain	26 – 88	63	t-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	50	1	Phosphoserine Ref.5 Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	O15155 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B83B76ACBB3DA57E	FASTA	118	13,289
	10 20 30 40 50 60 MRRAGLGEGV PPGNYGNYGY ANSGYSACEE ENERLTESLR SKVTAIKSLS IEIGHEVKTQ 70 80 90 100 110 NKLLAEMDSQ FDSTTGFLGK TMGKLKILSR GSQTKLLCYM MLFSLFVFFI IYWIIKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mammalian protein (rbet1) homologous to yeast Bet1p is primarily associated with the pre-Golgi intermediate compartment and is involved in vesicular transport from the endoplasmic reticulum to the Golgi apparatus." Zhang T., Wong S.H., Tang B.L., Xu Y., Peter F., Subramaniam V.N., Hong W. J. Cell Biol. 139:1157-1168(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF007551 mRNA. Translation: AAB62941.1.
AC006378 Genomic DNA. Translation: AAD47132.1.
BC000899 mRNA. Translation: AAH00899.1.

IPI

IPI00740027.

RefSeq

NP_005859.1. NM_005868.4.

UniGene

Hs.489132.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EGX	X-ray	3.30	D	24-32	[»]

ProteinModelPortal

O15155.

SMR

O15155. Positions 33-95.

ModBase

Search...

Protein-protein interaction databases

IntAct

O15155. 1 interaction.

STRING

9606.ENSP00000222547.

PTM databases

PhosphoSite

O15155.

Proteomic databases

PaxDb

O15155.

PRIDE

O15155.

Protocols and materials databases

DNASU

10282.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000222547; ENSP00000222547; ENSG00000105829.

GeneID

10282.

KEGG

hsa:10282.

UCSC

uc003unf.1. human.

Organism-specific databases

CTD

10282.

GeneCards

GC07M093592.

HGNC

HGNC:14562. BET1.

HPA

HPA042778.

MIM

605456. gene.

neXtProt

NX_O15155.

PharmGKB

PA25338.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG292170.

HOGENOM

HOG000290697.

HOVERGEN

HBG029661.

KO

K08504.

PhylomeDB

O15155.

Gene expression databases

ArrayExpress

O15155.

Bgee

O15155.

CleanEx

HS_BET1.

Genevestigator

O15155.

GermOnline

ENSG00000105829. Homo sapiens.

Family and domain databases

InterPro

IPR000727. T_SNARE_dom.
[Graphical view]

Pfam

PF05739. SNARE. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

PROSITE

PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O15155.

GenomeRNAi

10282.

NextBio

38956.

SOURCE

Search...

Entry information

Entry name

BET1_HUMAN

Accession

Primary (citable) accession number: O15155

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.