O15127 (SCAM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Secretory carrier-associated membrane protein 2
Short name=Secretory carrier membrane protein 2

Gene names

Name:

SCAMP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
Subunit structure	Interacts with SLC6A4 and SLC9A7. Ref.6 Ref.7
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein Ref.6.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the SCAMP family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	post-Golgi vesicle-mediated transport Traceable author statement Ref.1. Source: ProtInc protein transport Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA integral to membrane Inferred from direct assay Ref.6. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA recycling endosome membrane Inferred from direct assay Ref.6. Source: UniProtKB trans-Golgi network membrane Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Secretory carrier-associated membrane protein 2		PRO_0000191254
Regions
	Topological domain	1 – 153	153	Cytoplasmic Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Topological domain	175 – 181	7	Lumenal Potential
	Transmembrane	182 – 202	21	Helical; Potential
	Topological domain	203 – 218	16	Cytoplasmic Potential
	Transmembrane	219 – 239	21	Helical; Potential
	Topological domain	240 – 262	23	Lumenal Potential
	Transmembrane	263 – 283	21	Helical; Potential
	Topological domain	284 – 329	46	Cytoplasmic Potential
	Region	203 – 218	16	Interaction with SLC9A7
Amino acid modifications
	Modified residue	319	1	Phosphoserine Ref.9
	Modified residue	320	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	O15127 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 046ACB926FD951DD	FASTA	329	36,649
	10 20 30 40 50 60 MSAFDTNPFA DPVDVNPFQD PSVTQLTNAP QGGLAEFNPF SETNAATTVP VTQLPGSSQP 70 80 90 100 110 120 AVLQPSVEPT QPTPQAVVSA AQAGLLRQQE ELDRKAAELE RKERELQNTV ANLHVRQNNW 130 140 150 160 170 180 PPLPSWCPVK PCFYQDFSTE IPADYQRICK MLYYLWMLHS VTLFLNLLAC LAWFSGNSSK 190 200 210 220 230 240 GVDFGLSILW FLIFTPCAFL CWYRPIYKAF RSDNSFSFFV FFFVFFCQIG IYIIQLVGIP 250 260 270 280 290 300 GLGDSGWIAA LSTLDNHSLA ISVIMMVVAG FFTLCAVLSV FLLQRVHSLY RRTGASFQQA 310 320 QEEFSQGIFS SRTFHRAASS AAQGAFQGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions." Singleton D.R., Wu T.T., Castle J.D. J. Cell Sci. 110:2099-2107(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Singleton D.R., Wu T.T., Castle J.D. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7." Lin P.J., Williams W.P., Luu Y., Molday R.S., Orlowski J., Numata M. J. Cell Sci. 118:1885-1897(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLC9A7.
[7]	"Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2." Mueller H.K., Wiborg O., Haase J. J. Biol. Chem. 281:28901-28909(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SLC6A4.
[8]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Platelet.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005038 mRNA. Translation: AAB62723.2. AK315516 mRNA. Translation: BAG37897.1. CH471136 Genomic DNA. Translation: EAW99300.1. BC001376 mRNA. Translation: AAH01376.1. BC004385 mRNA. Translation: AAH04385.1.
IPI	IPI00218850.
RefSeq	NP_005688.2. NM_005697.3.
UniGene	Hs.458917.
3D structure databases
ProteinModelPortal	O15127.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15127. 3 interactions.
MINT	MINT-5003778.
STRING	9606.ENSP00000268099.
PTM databases
PhosphoSite	O15127.
Proteomic databases
PaxDb	O15127.
PeptideAtlas	O15127.
PRIDE	O15127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000268099; ENSP00000268099; ENSG00000140497.
GeneID	10066.
KEGG	hsa:10066.
UCSC	uc002azb.1. human.
Organism-specific databases
CTD	10066.
GeneCards	GC15M075136.
HGNC	HGNC:10564. SCAMP2.
HPA	HPA014699. HPA019194.
MIM	606912. gene.
neXtProt	NX_O15127.
PharmGKB	PA34977.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG285953.
HOGENOM	HOG000294221.
HOVERGEN	HBG071938.
InParanoid	O15127.
OMA	QGIFSNR.
OrthoDB	EOG4Z8XXB.
PhylomeDB	O15127.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6_traffickingpathway. Arf6 trafficking events.
Gene expression databases
ArrayExpress	O15127.
Bgee	O15127.
CleanEx	HS_SCAMP2.
Genevestigator	O15127.
GermOnline	ENSG00000140497. Homo sapiens.
Family and domain databases
InterPro	IPR007273. SCAMP. [Graphical view]
PANTHER	PTHR10687. PTHR10687. 1 hit.
Pfam	PF04144. SCAMP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SCAMP2. human.
GenomeRNAi	10066.
NextBio	38041.
SOURCE	Search...

Entry information

Entry name

SCAM2_HUMAN

Accession

Primary (citable) accession number: O15127
Secondary accession number(s): B2RDF0, Q9BQE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 11, 2001
Last modified:	April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.