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Protein

Secretory carrier-associated membrane protein 1

Gene

SCAMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.

GO - Biological processi

  • exocytosis Source: Ensembl
  • post-Golgi vesicle-mediated transport Source: ProtInc
  • protein transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Secretory carrier-associated membrane protein 1
Short name:
Secretory carrier membrane protein 1
Gene namesi
Name:SCAMP1
Synonyms:SCAMP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:10563. SCAMP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 155154CytoplasmicSequence analysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence analysisAdd
BLAST
Topological domaini177 – 1815LumenalSequence analysis
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Topological domaini203 – 21816CytoplasmicSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST
Topological domaini240 – 26122LumenalSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Topological domaini283 – 33856CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: HPA
  • clathrin-coated vesicle Source: ParkinsonsUK-UCL
  • integral component of membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA
  • recycling endosome membrane Source: UniProtKB
  • synaptic vesicle membrane Source: Ensembl
  • trans-Golgi network Source: UniProtKB
  • zymogen granule membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34976.

Polymorphism and mutation databases

BioMutaiSCAMP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 338337Secretory carrier-associated membrane protein 1PRO_0000191250Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei2 – 21PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO15126.
MaxQBiO15126.
PRIDEiO15126.

PTM databases

iPTMnetiO15126.
PhosphoSiteiO15126.
SwissPalmiO15126.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in brain.

Gene expression databases

CleanExiHS_SCAMP1.
ExpressionAtlasiO15126. baseline and differential.
GenevisibleiO15126. HS.

Interactioni

Subunit structurei

Interacts with SYNRG and ITSN1 (By similarity). Interacts with SLC9A7.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FAM208BQ5VWN6-23EBI-954338,EBI-10172380
ZMIZ2Q8NF64-23EBI-954338,EBI-10182121

Protein-protein interaction databases

BioGridi114899. 30 interactions.
IntActiO15126. 8 interactions.
MINTiMINT-2866902.

Structurei

3D structure databases

ProteinModelPortaliO15126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SCAMP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000014393.
HOGENOMiHOG000207630.
HOVERGENiHBG071938.
InParanoidiO15126.
KOiK19995.
OMAiQSAFKGN.
PhylomeDBiO15126.

Family and domain databases

InterProiIPR007273. SCAMP.
[Graphical view]
PANTHERiPTHR10687. PTHR10687. 1 hit.
PfamiPF04144. SCAMP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15126-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDFDSNPFA DPDLNNPFKD PSVTQVTRNV PPGLDEYNPF SDSRTPPPGG
60 70 80 90 100
VKMPNVPNTQ PAIMKPTEEH PAYTQIAKEH ALAQAELLKR QEELERKAAE
110 120 130 140 150
LDRREREMQN LSQHGRKNNW PPLPSNFPVG PCFYQDFSVD IPVEFQKTVK
160 170 180 190 200
LMYYLWMFHA VTLFLNIFGC LAWFCVDSAR AVDFGLSILW FLLFTPCSFV
210 220 230 240 250
CWYRPLYGAF RSDSSFRFFV FFFVYICQFA VHVLQAAGFH NWGNCGWISS
260 270 280 290 300
LTGLNQNIPV GIMMIIIAAL FTASAVISLV MFKKVHGLYR TTGASFEKAQ
310 320 330
QEFATGVMSN KTVQTAAANA ASTAASSAAQ NAFKGNQI
Length:338
Mass (Da):37,920
Last modified:June 20, 2002 - v2
Checksum:iC5745732744BE3C7
GO
Isoform 2 (identifier: O15126-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-338: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:157
Mass (Da):18,042
Checksum:i2A9462644E1AD684
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191N → I in AAC39864 (PubMed:9653160).Curated
Sequence conflicti136 – 1361D → E in AAC39864 (PubMed:9653160).Curated
Sequence conflicti247 – 2471W → L in AAB62722 (PubMed:9378760).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei158 – 338181Missing in isoform 2. 1 PublicationVSP_004380Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005037 mRNA. Translation: AAB62722.1.
AF038966 mRNA. Translation: AAC39864.1.
CR542286 mRNA. Translation: CAG47081.1.
BC009787 mRNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95814.1.
BC015065 mRNA. Translation: AAH15065.1.
BC034048 mRNA. Translation: AAH34048.1.
CCDSiCCDS75264.1. [O15126-1]
RefSeqiNP_001277158.1. NM_001290229.1.
NP_004857.4. NM_004866.5. [O15126-1]
UniGeneiHs.482587.

Genome annotation databases

EnsembliENST00000614488; ENSP00000478071; ENSG00000085365. [O15126-2]
ENST00000621999; ENSP00000481022; ENSG00000085365. [O15126-1]
GeneIDi9522.
KEGGihsa:9522.
UCSCiuc032uzx.2. human. [O15126-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005037 mRNA. Translation: AAB62722.1.
AF038966 mRNA. Translation: AAC39864.1.
CR542286 mRNA. Translation: CAG47081.1.
BC009787 mRNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95814.1.
BC015065 mRNA. Translation: AAH15065.1.
BC034048 mRNA. Translation: AAH34048.1.
CCDSiCCDS75264.1. [O15126-1]
RefSeqiNP_001277158.1. NM_001290229.1.
NP_004857.4. NM_004866.5. [O15126-1]
UniGeneiHs.482587.

3D structure databases

ProteinModelPortaliO15126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114899. 30 interactions.
IntActiO15126. 8 interactions.
MINTiMINT-2866902.

PTM databases

iPTMnetiO15126.
PhosphoSiteiO15126.
SwissPalmiO15126.

Polymorphism and mutation databases

BioMutaiSCAMP1.

Proteomic databases

EPDiO15126.
MaxQBiO15126.
PRIDEiO15126.

Protocols and materials databases

DNASUi9522.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000614488; ENSP00000478071; ENSG00000085365. [O15126-2]
ENST00000621999; ENSP00000481022; ENSG00000085365. [O15126-1]
GeneIDi9522.
KEGGihsa:9522.
UCSCiuc032uzx.2. human. [O15126-1]

Organism-specific databases

CTDi9522.
GeneCardsiSCAMP1.
HGNCiHGNC:10563. SCAMP1.
MIMi606911. gene.
neXtProtiNX_O15126.
PharmGKBiPA34976.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000014393.
HOGENOMiHOG000207630.
HOVERGENiHBG071938.
InParanoidiO15126.
KOiK19995.
OMAiQSAFKGN.
PhylomeDBiO15126.

Miscellaneous databases

GeneWikiiSCAMP1.
GenomeRNAii9522.
NextBioi35688.
PROiO15126.
SOURCEiSearch...

Gene expression databases

CleanExiHS_SCAMP1.
ExpressionAtlasiO15126. baseline and differential.
GenevisibleiO15126. HS.

Family and domain databases

InterProiIPR007273. SCAMP.
[Graphical view]
PANTHERiPTHR10687. PTHR10687. 1 hit.
PfamiPF04144. SCAMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions."
    Singleton D.R., Wu T.T., Castle J.D.
    J. Cell Sci. 110:2099-2107(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
    Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.
    Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Bone marrow, Brain and Prostate.
  6. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
  7. "Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7."
    Lin P.J., Williams W.P., Luu Y., Molday R.S., Orlowski J., Numata M.
    J. Cell Sci. 118:1885-1897(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLC9A7.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSCAM1_HUMAN
AccessioniPrimary (citable) accession number: O15126
Secondary accession number(s): O43587
, Q6FG23, Q96BX1, Q96QK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 20, 2002
Last modified: May 11, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.