O15066 (KIF3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF3B

Alternative name(s):
HH0048
Microtubule plus end-directed kinesin motor 3B

Gene names

Name:	KIF3B
Synonyms:	KIAA0359

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro By similarity.
Subunit structure	Heterodimer of KIF3A and KIF3B. Interacts directly with IFT20 By similarity. Interacts with the SMC3 subunit of the cohesin complex. Ref.5
Subcellular location	Cytoplasm › cytoskeleton Probable.
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.
Sequence caution	The sequence BAA20815.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterograde axon cargo transport Traceable author statement PubMed 7559760. Source: ProtInc antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome blood coagulation Traceable author statement. Source: Reactome determination of left/right symmetry Traceable author statement PubMed 9865700. Source: ProtInc mitotic centrosome separation Traceable author statement PubMed 16298999. Source: BHF-UCL plus-end-directed vesicle transport along microtubule Traceable author statement PubMed 16298999. Source: BHF-UCL spindle assembly involved in mitosis Inferred from mutant phenotype PubMed 19635168. Source: BHF-UCL
Cellular_component	centrosome Non-traceable author statement PubMed 16298999. Source: BHF-UCL cytosol Traceable author statement. Source: Reactome kinesin II complex Inferred from direct assay PubMed 16298999. Source: BHF-UCL plus-end kinesin complex Traceable author statement PubMed 7559760. Source: ProtInc spindle microtubule Non-traceable author statement PubMed 16298999. Source: BHF-UCL
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW plus-end-directed microtubule motor activity Traceable author statement PubMed 7559760. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 747

747

Kinesin-like protein KIF3B

PRO_0000125395

Regions

Domain

1 – 345

345

Kinesin-motor

Nucleotide binding

96 – 103

8

ATP

Region

580 – 747

168

Globular

Coiled coil

346 – 579

234

Potential

Compositional bias

386 – 393

8

Poly-Gly

Compositional bias

394 – 406

13

Poly-Glu

Compositional bias

723 – 730

8

Poly-Ser

Secondary structure

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747

Helix Strand Turn

Details...

Beta strand

11 – 16

6

Helix

21 – 25

5

Beta strand

32 – 35

4

Turn

36 – 39

4

Beta strand

40 – 43

4

Turn

49 – 51

3

Beta strand

55 – 58

4

Beta strand

60 – 63

4

Helix

69 – 75

7

Helix

77 – 85

9

Beta strand

90 – 97

8

Helix

102 – 106

5

Helix

113 – 115

3

Helix

118 – 131

14

Beta strand

137 – 148

12

Beta strand

151 – 154

4

Beta strand

166 – 169

4

Turn

170 – 172

3

Beta strand

173 – 176

4

Helix

187 – 201

15

Turn

202 – 204

3

Helix

208 – 213

6

Beta strand

215 – 227

13

Beta strand

235 – 245

11

Beta strand

261 – 264

4

Helix

265 – 267

3

Helix

270 – 283

14

Helix

292 – 294

3

Helix

296 – 300

5

Turn

301 – 306

6

Beta strand

307 – 317

11

Helix

321 – 323

3

Helix

324 – 337

14

Sequences

	Sequence		Length	Mass (Da)
	O15066 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 97FA4573AFA87023	FASTA	747	85,125
	10 20 30 40 50 60 MSKLKSSESV RVVVRCRPMN GKEKAASYDK VVDVDVKLGQ VSVKNPKGTA HEMPKTFTFD 70 80 90 100 110 120 AVYDWNAKQF ELYDETFRPL VDSVLQGFNG TIFAYGQTGT GKTYTMEGIR GDPEKRGVIP 130 140 150 160 170 180 NSFDHIFTHI SRSQNQQYLV RASYLEIYQE EIRDLLSKDQ TKRLELKERP DTGVYVKDLS 190 200 210 220 230 240 SFVTKSVKEI EHVMNVGNQN RSVGATNMNE HSSRSHAIFV ITIECSEVGL DGENHIRVGK 250 260 270 280 290 300 LNLVDLAGSE RQAKTGAQGE RLKEATKINL SLSALGNVIS ALVDGKSTHI PYRDSKLTRL 310 320 330 340 350 360 LQDSLGGNAK TVMVANVGPA SYNVEETLTT LRYANRAKNI KNKPRVNEDP KDALLREFQE 370 380 390 400 410 420 EIARLKAQLE KRSIGRRKRR EKRREGGGSG GGGEEEEEEG EEGEEEGDDK DDYWREQQEK 430 440 450 460 470 480 LEIEKRAIVE DHSLVAEEKM RLLKEKEKKM EDLRREKDAA EMLGAKIKAM ESKLLVGGKN 490 500 510 520 530 540 IVDHTNEQQK ILEQKRQEIA EQKRREREIQ QQMESRDEET LELKETYSSL QQEVDIKTKK 550 560 570 580 590 600 LKKLFSKLQA VKAEIHDLQE EHIKERQELE QTQNELTREL KLKHLIIENF IPLEEKSKIM 610 620 630 640 650 660 NRAFFDEEED HWKLHPITRL ENQQMMKRPV SAVGYKRPLS QHARMSMMIR PEARYRAENI 670 680 690 700 710 720 VLLELDMPSR TTRDYEGPAI APKVQAALDA ALQDEDEIQV DASSFESTAN KKSKARPKSG 730 740 RKSGSSSSSS GTPASQLYPQ SRGLVPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis.
[5]	"Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide." Shimizu K., Shirataki H., Honda T., Minami S., Takai Y. J. Biol. Chem. 273:6591-6594(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH SMC3 AND KIFAP3B.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Motor domain of human kinesin family member 3B in complex with ADP." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 6-359IN COMPLEX WITH ADP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB002357 mRNA. Translation: BAA20815.2. Different initiation.
AL121897 Genomic DNA. Translation: CAC16425.1.
CH471077 Genomic DNA. Translation: EAW76381.1.
CH471077 Genomic DNA. Translation: EAW76382.1.
BC136310 mRNA. Translation: AAI36311.1.
BC136311 mRNA. Translation: AAI36312.1.

IPI

IPI00004533.

RefSeq

NP_004789.1. NM_004798.3.

UniGene

Hs.369670.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B6U	X-ray	1.80	A/B	6-359	[»]

ProteinModelPortal

O15066.

ModBase

Search...

Protein-protein interaction databases

IntAct

O15066. 1 interaction.

MINT

MINT-1185610.

STRING

9606.ENSP00000364864.

PTM databases

PhosphoSite

O15066.

Proteomic databases

PaxDb

O15066.

PeptideAtlas

O15066.

PRIDE

O15066.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000375712; ENSP00000364864; ENSG00000101350.

GeneID

9371.

KEGG

hsa:9371.

UCSC

uc002wxq.3. human.

Organism-specific databases

CTD

9371.

GeneCards

GC20P030865.

HGNC

HGNC:6320. KIF3B.

HPA

HPA007119.

MIM

603754. gene.

neXtProt

NX_O15066.

PharmGKB

PA30103.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5059.

HOGENOM

HOG000116164.

HOVERGEN

HBG052255.

InParanoid

O15066.

KO

K10394.

OMA

EIYMEEL.

OrthoDB

EOG4J3WGM.

PhylomeDB

O15066.

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.
REACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

ArrayExpress

O15066.

Bgee

O15066.

CleanEx

HS_KIF3B.

Genevestigator

O15066.

GermOnline

ENSG00000101350. Homo sapiens.

Family and domain databases

Gene3D

3.40.850.10. 1 hit.

InterPro

IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
[Graphical view]

Pfam

PF00225. Kinesin. 1 hit.
[Graphical view]

PRINTS

PR00380. KINESINHEAVY.

SMART

SM00129. KISc. 1 hit.
[Graphical view]

PROSITE

PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

O15066.

ChEMBL

CHEMBL6109.

ChiTaRS

KIF3B. human.

EvolutionaryTrace

O15066.

GenomeRNAi

9371.

NextBio

35098.

SOURCE

Search...

Entry information

Entry name

KIF3B_HUMAN

Accession

Primary (citable) accession number: O15066
Secondary accession number(s): B2RMP4, E1P5M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.