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O15056 (SYNJ2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptojanin-2

EC=3.1.3.36
Alternative name(s):
Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2
Gene names
Name:SYNJ2
Synonyms:KIAA0348
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

Subunit structure

Binds to GRB2. Isoform 2A binds to SYNJ2BP/OMP25. Isoform 2B2 C-terminal proline-rich region binds to a variety of SH3 domain-containing proteins including SH3GL1, SH3GL2, SH3GL3 and GRB2.

Subcellular location

Membrane By similarity. Note: Predominantly associated with the particulate fractions By similarity. Ref.2

Sequence similarities

Belongs to the synaptojanin family.

In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.

Contains 1 RRM (RNA recognition motif) domain.

Contains 1 SAC domain.

Sequence caution

The sequence AAH43277.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 2B2 (identifier: O15056-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2B1 (identifier: O15056-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1070-1114: Missing.
Note: No experimental confirmation available.
Isoform 2A (identifier: O15056-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1249-1288: PLSPEEQFEQ...TAPRVPPVPK → IVFCSRSQAS...ASVDTSGSSV
     1289-1496: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14961496Synaptojanin-2
PRO_0000209733

Regions

Domain120 – 444325SAC
Domain889 – 96880RRM
Region450 – ?Catalytic By similarity
Compositional bias1099 – 111416Pro-rich
Compositional bias1209 – 1334126Pro-rich

Natural variations

Alternative sequence1070 – 111445Missing in isoform 2B1.
VSP_012911
Alternative sequence1249 – 128840PLSPE…PPVPK → IVFCSRSQASQPCLLLQRHE FVRTVAAQRLASVDTSGSSV in isoform 2A.
VSP_012912
Alternative sequence1289 – 1496208Missing in isoform 2A.
VSP_012913
Natural variant14681E → G. Ref.6
Corresponds to variant rs2502601 [ dbSNP | Ensembl ].
VAR_024507

Secondary structure

................. 1496
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 2B2 [UniParc].

Last modified March 1, 2005. Version 3.
Checksum: 7CC9E368585ACF21

FASTA1,496165,538
        10         20         30         40         50         60 
MALSKGLRLL GRLGAEGDCS VLLEARGRDD CLLFEAGTVA TLAPEEKEVI KGQYGKLTDA 

        70         80         90        100        110        120 
YGCLGELRLK SGGTSLSFLV LVTGCTSVGR IPDAEIYKIT ATDFYPLQEE AKEEERLIAL 

       130        140        150        160        170        180 
KKILSSGVFY FSWPNDGSRF DLTVRTQKQG DDSSEWGNSF FWNQLLHVPL RQHQVSCCDW 

       190        200        210        220        230        240 
LLKIICGVVT IRTVYASHKQ AKACLVSRVS CERTGTRFHT RGVNDDGHVS NFVETEQMIY 

       250        260        270        280        290        300 
MDDGVSSFVQ IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFDRHMVL LKEQYGQQVV 

       310        320        330        340        350        360 
VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGGKLEKLE TLLRPQLKLH 

       370        380        390        400        410        420 
WEDFDVFTKG ENVSPRFQKG TLRMNCLDCL DRTNTVQSFI ALEVLHLQLK TLGLSSKPIV 

       430        440        450        460        470        480 
DRFVESFKAM WSLNGHSLSK VFTGSRALEG KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA 

       490        500        510        520        530        540 
IKLLLVGDVY GEEVADKGGM LLDSTALLVT PRILKAMTER QSEFTNFKRI RIAMGTWNVN 

       550        560        570        580        590        600 
GGKQFRSNVL RTAELTDWLL DSPQLSGATD SQDDSSPADI FAVGFEEMVE LSAGNIVNAS 

       610        620        630        640        650        660 
TTNKKMWGEQ LQKAISRSHR YILLTSAQLV GVCLYIFVRP YHVPFIRDVA IDTVKTGMGG 

       670        680        690        700        710        720 
KAGNKGAVGI RFQFHSTSFC FICSHLTAGQ SQVKERNEDY KEITQKLCFP MGRNVFSHDY 

       730        740        750        760        770        780 
VFWCGDFNYR IDLTYEEVFY FVKRQDWKKL LEFDQLQLQK SSGKIFKDFH EGAINFGPTY 

       790        800        810        820        830        840 
KYDVGSAAYD TSDKCRTPAW TDRVLWWRKK HPFDKTAGEL NLLDSDLDVD TKVRHTWSPG 

       850        860        870        880        890        900 
ALQYYGRAEL QASDHRPVLA IVEVEVQEVD VGARERVFQE VSSFQGPLDA TVVVNLQSPT 

       910        920        930        940        950        960 
LEEKNEFPED LRTELMQTLG SYGTIVLVRI NQGQMLVTFA DSHSALSVLD VDGMKVKGRA 

       970        980        990       1000       1010       1020 
VKIRPKTKDW LKGLREEIIR KRDSMAPVSP TANSCLLEEN FDFTSLDYES EGDILEDDED 

      1030       1040       1050       1060       1070       1080 
YLVDEFNQPG VSDSELGGDD LSDVPGPTAL APPSKSPALT KKKQHPTYKD DADLVELKRE 

      1090       1100       1110       1120       1130       1140 
LEAVGEFRHR SPSRSLSVPN RPRPPQPPQR PPPPTGLMVK KSASDASISS GTHGQYSILQ 

      1150       1160       1170       1180       1190       1200 
TARLLPGAPQ QPPKARTGIS KPYNVKQIKT TNAQEAEAAI RCLLEARGGA SEEALSAVAP 

      1210       1220       1230       1240       1250       1260 
RDLEASSEPE PTPGAAKPET PQAPPLLPRR PPPRVPAIKK PTLRRTGKPL SPEEQFEQQT 

      1270       1280       1290       1300       1310       1320 
VHFTIGPPET SVEAPPVVTA PRVPPVPKPR TFQPGKAAER PSHRKPASDE APPGAGASVP 

      1330       1340       1350       1360       1370       1380 
PPLEAPPLVP KVPPRRKKSA PAAFHLQVLQ SNSQLLQGLT YNSSDSPSGH PPAAGTVFPQ 

      1390       1400       1410       1420       1430       1440 
GDFLSTSSAT SPDSDGTKAM KPEAAPLLGD YQDPFWNLLH HPKLLNNTWL SKSSDPLDSG 

      1450       1460       1470       1480       1490 
TRSPKRDPID PVSAGASAAK AELPPDHEHK TLGHWVTISD QEKRTALQVF DPLAKT 

« Hide

Isoform 2B1 [UniParc].

Checksum: 42E19A35A791EA6A
Show »

FASTA1,451160,434
Isoform 2A [UniParc].

Checksum: 9A4EAA3AA9AE2451
Show »

FASTA1,288143,445

References

« Hide 'large scale' references
[1]"cDNA cloning of human SH3 domain-containing proteins, synaptojanin 2A."
Jeong S.-Y., Youle R.J.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2A).
[2]"Synaptojanin 2, a novel Rac1 effector that regulates clathrin-mediated endocytosis."
Malecz N., McCabe P.C., Spaargaren C., Qiu R.-G., Chuang Y.-Y., Symons M.
Curr. Biol. 10:1383-1386(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2B2), SUBCELLULAR LOCATION.
[3]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals."
Nemoto Y., Wenk M.R., Watanabe M., Daniell L., Murakami T., Ringstad N., Yamada H., Takei K., De Camilli P.
J. Biol. Chem. 276:41133-41142(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 54-1496 (ISOFORM 2B2).
Tissue: Brain.
[5]"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-1496 (ISOFORM 2B2).
Tissue: Brain.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 647-1496 (ISOFORM 2B2), VARIANT GLY-1468.
Tissue: Testis.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Solution structure of RNP domain in synaptojanin 2."
RIKEN structural genomics initiative (RSGI)
Submitted (DEC-2003) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 819-969.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY152396 mRNA. Translation: AAN73051.1.
AF318616 mRNA. Translation: AAG46036.1.
AL139330 Genomic DNA. Translation: CAI12978.1.
AL139330 Genomic DNA. Translation: CAI12983.1.
AL139330 Genomic DNA. Translation: CAI12984.1.
AF039945 mRNA. Translation: AAD02178.1.
AB002346 mRNA. Translation: BAA20805.2.
BC043277 mRNA. Translation: AAH43277.1. Different initiation.
RefSeqNP_003889.1. NM_003898.3.
UniGeneHs.434494.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UFWNMR-A882-963[»]
ProteinModelPortalO15056.
SMRO15056. Positions 37-446, 517-881, 884-963.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114391. 10 interactions.
IntActO15056. 10 interactions.
MINTMINT-3976061.
STRING9606.ENSP00000347792.

PTM databases

PhosphoSiteO15056.

Proteomic databases

PaxDbO15056.
PRIDEO15056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000355585; ENSP00000347792; ENSG00000078269. [O15056-1]
ENST00000367121; ENSP00000356088; ENSG00000078269. [O15056-3]
GeneID8871.
KEGGhsa:8871.
UCSCuc003qqw.2. human. [O15056-3]
uc003qqx.2. human. [O15056-1]

Organism-specific databases

CTD8871.
GeneCardsGC06P158402.
HGNCHGNC:11504. SYNJ2.
HPAHPA031575.
HPA031953.
MIM609410. gene.
neXtProtNX_O15056.
PharmGKBPA36286.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5411.
HOVERGENHBG079225.
InParanoidO15056.
KOK01099.
OMAVIKGQYG.
OrthoDBEOG7N63KS.
PhylomeDBO15056.
TreeFamTF354311.

Enzyme and pathway databases

BioCycMetaCyc:HS01279-MONOMER.
ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressO15056.
BgeeO15056.
CleanExHS_SYNJ2.
GenevestigatorO15056.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
3.60.10.10. 1 hit.
InterProIPR015047. DUF1866.
IPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR002013. Syja_N.
[Graphical view]
PfamPF08952. DUF1866. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 2 hits.
PROSITEPS50102. RRM. 1 hit.
PS50275. SAC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO15056.
GenomeRNAi8871.
NextBio33305.
PROO15056.
SOURCESearch...

Entry information

Entry nameSYNJ2_HUMAN
AccessionPrimary (citable) accession number: O15056
Secondary accession number(s): Q5TA13 expand/collapse secondary AC list , Q5TA16, Q5TA19, Q86XK0, Q8IZA8, Q9H226
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM