O15049 (N4BP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD4-binding protein 3
Short name=N4BP3

Gene names

Name:	N4BP3
Synonyms:	KIAA0341

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Binds NEDD4. Interacts with 14-3-3 proteins. Ref.5 Ref.6
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein Ref.5.
Sequence caution	The sequence BAA20799.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAC86891.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC86891.1 differs from that shown. Reason: Intron retention. The sequence BAG58525.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	NEDD4-binding protein 3		PRO_0000096682
Regions
	Coiled coil	294 – 530	237	Potential
	Compositional bias	242 – 247	6	Poly-Pro
Amino acid modifications
	Modified residue	176	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	29	1	L → P in BAA20799. Ref.1
	Sequence conflict	163	1	R → Q in AAH53323. Ref.3
	Sequence conflict	317	1	R → H in BAC86891. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O15049 [UniParc]. Last modified April 17, 2007. Version 3. Checksum: 40F2652D0848C27C	FASTA	544	60,470
	10 20 30 40 50 60 MATAPGPAGI AMGSVGSLLE RQDFSPEELR AALAGSRGSR QPDGLLRKGL GQREFLSYLH 70 80 90 100 110 120 LPKKDSKSTK NTKRAPRNEP ADYATLYYRE HSRAGDFSKT SLPERGRFDK CRIRPSVFKP 130 140 150 160 170 180 TAGNGKGFLS MQSLASHKGQ KLWRSNGSLH TLACHPPLSP GPRASQARAQ LLHALSLDEG 190 200 210 220 230 240 GPEPEPSLSD SSSGGSFGRS PGTGPSPFSS SLGHLNHLGG SLDRASQGPK EAGPPAVLSC 250 260 270 280 290 300 LPEPPPPYEF SCSSAEEMGA VLPETCEELK RGLGDEDGSN PFTQVLEERQ RLWLAELKRL 310 320 330 340 350 360 YVERLHEVTQ KAERSERNLQ LQLFMAQQEQ RRLRKELRAQ QGLAPEPRAP GTLPEADPSA 370 380 390 400 410 420 RPEEEARWEV CQKTAEISLL KQQLREAQAE LAQKLAEIFS LKTQLRGSRA QAQAQDAELV 430 440 450 460 470 480 RLREAVRSLQ EQAPREEAPG SCETDDCKSR GLLGEAGGSE ARDSAEQLRA ELLQERLRGQ 490 500 510 520 530 540 EQALRFEQER RTWQEEKERV LRYQREIQGG YMDMYRRNQA LEQELRALRE PPTPWSPRLE SSKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 285-544. Tissue: Hippocampus.
[5]	"Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase." Murillas R., Simms K.S., Hatakeyama S., Weissman A.M., Kuehn M.R. J. Biol. Chem. 277:2897-2907(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NEDD4.
[6]	"Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization." Jin J., Smith F.D., Stark C., Wells C.D., Fawcett J.P., Kulkarni S., Metalnikov P., O'Donnell P., Taylor P., Taylor L., Zougman A., Woodgett J.R., Langeberg L.K., Scott J.D., Pawson T. Curr. Biol. 14:1436-1450(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH 14-3-3 PROTEINS.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB002339 mRNA. Translation: BAA20799.1. Different initiation. CH471165 Genomic DNA. Translation: EAW53851.1. CH471165 Genomic DNA. Translation: EAW53852.1. CH471165 Genomic DNA. Translation: EAW53853.1. CH471165 Genomic DNA. Translation: EAW53854.1. BC053323 mRNA. Translation: AAH53323.2. AK127224 mRNA. Translation: BAC86891.1. Sequence problems. AK295663 mRNA. Translation: BAG58525.1. Different initiation.
IPI	IPI00004500.
RefSeq	NP_055926.1. NM_015111.1.
UniGene	Hs.101761.
3D structure databases
ProteinModelPortal	O15049.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15049. 1 interaction.
MINT	MINT-222654.
PTM databases
PhosphoSite	O15049.
Proteomic databases
PaxDb	O15049.
PRIDE	O15049.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274605; ENSP00000274605; ENSG00000145911.
GeneID	23138.
KEGG	hsa:23138.
UCSC	uc003mik.1. human.
Organism-specific databases
CTD	23138.
GeneCards	GC05P177540.
H-InvDB	HIX0005472.
HGNC	HGNC:29852. N4BP3.
HPA	HPA030973.
neXtProt	NX_O15049.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG75546.
HOGENOM	HOG000230889.
HOVERGEN	HBG052566.
InParanoid	O15049.
OMA	QALRFEQ.
OrthoDB	EOG42RD7V.
PhylomeDB	O15049.
Gene expression databases
Bgee	O15049.
Genevestigator	O15049.
GermOnline	ENSG00000145911. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	23138.
NextBio	44407.

Entry information

Entry name

N4BP3_HUMAN

Accession

Primary (citable) accession number: O15049
Secondary accession number(s): B4DIL3 , D3DWP3, Q6ZSQ6, Q7Z6I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	April 17, 2007
Last modified:	May 1, 2013
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.