O15042 (SR140_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U2 snRNP-associated SURP motif-containing protein

Alternative name(s):
140 kDa Ser/Arg-rich domain protein
U2-associated protein SR140

Gene names

Name:	U2SURP
Synonyms:	KIAA0332, SR140

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1029 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with ERBB4. Ref.9
Subcellular location	Nucleus Ref.9.
Sequence similarities	Belongs to the splicing factor SR family. Contains 1 CID domain. Contains 1 RRM (RNA recognition motif) domain. Contains 1 SURP motif repeat.
Sequence caution	The sequence AAH16323.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAI05605.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from direct assay Ref.9. Source: UniProtKB
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O15042-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O15042-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: Missing.

Isoform 3 (identifier: O15042-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-409: Missing.
     410-424: KTLSQAIVKVVIPTE → MLLCYRHLKTKRILR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1029	1029	U2 snRNP-associated SURP motif-containing protein		PRO_0000280070
Regions
	Domain	274 – 355	82	RRM
	Repeat	430 – 473	44	SURP motif
	Domain	534 – 679	146	CID
	Coiled coil	92 – 121	30	Potential
	Coiled coil	192 – 232	41	Potential
	Coiled coil	837 – 915	79	Potential
	Compositional bias	357 – 402	46	Pro-rich
	Compositional bias	689 – 746	58	Asp-rich
	Compositional bias	762 – 917	156	Glu-rich
	Compositional bias	922 – 1001	80	Arg/Ser-rich
Amino acid modifications
	Modified residue	202	1	Phosphoserine Ref.12
	Modified residue	485	1	Phosphoserine Ref.10 Ref.12
	Modified residue	760	1	N6-acetyllysine Ref.8
	Modified residue	788	1	Phosphoserine Ref.6 Ref.7 Ref.12
	Modified residue	800	1	Phosphoserine Ref.6 Ref.7
	Modified residue	811	1	Phosphoserine Ref.5 Ref.10 Ref.12
	Modified residue	931	1	Phosphothreonine Ref.10
	Modified residue	946	1	Phosphoserine Ref.10
	Modified residue	948	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	1 – 409	409	Missing in isoform 3.		VSP_023522
	Alternative sequence	256	1	Missing in isoform 2.		VSP_023523
	Alternative sequence	410 – 424	15	KTLSQ…VIPTE → MLLCYRHLKTKRILR in isoform 3.		VSP_023524

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: 7AB9235C63299714	FASTA	1,029	118,292
	10 20 30 40 50 60 MADKTPGGSQ KASSKTRSSD VHSSGSSDAH MDASGPSDSD MPSRTRPKSP RKHNYRNESA 70 80 90 100 110 120 RESLCDSPHQ NLSRPLLENK LKAFSIGKMS TAKRTLSKKE QEELKKKEDE KAAAEIYEEF 130 140 150 160 170 180 LAAFEGSDGN KVKTFVRGGV VNAAKEEHET DEKRGKIYKP SSRFADQKNP PNQSSNERPP 190 200 210 220 230 240 SLLVIETKKP PLKKGEKEKK KSNLELFKEE LKQIQEERDE RHKTKGRLSR FEPPQSDSDG 250 260 270 280 290 300 QRRSMDAPSR RNRSSGVLDD YAPGSHDVGD PSTTNLYLGN INPQMNEEML CQEFGRFGPL 310 320 330 340 350 360 ASVKIMWPRT DEERARERNC GFVAFMNRRD AERALKNLNG KMIMSFEMKL GWGKAVPIPP 370 380 390 400 410 420 HPIYIPPSMM EHTLPPPPSG LPFNAQPRER LKNPNAPMLP PPKNKEDFEK TLSQAIVKVV 430 440 450 460 470 480 IPTERNLLAL IHRMIEFVVR EGPMFEAMIM NREINNPMFR FLFENQTPAH VYYRWKLYSI 490 500 510 520 530 540 LQGDSPTKWR TEDFRMFKNG SFWRPPPLNP YLHGMSEEQE TEAFVEEPSK KGALKEEQRD 550 560 570 580 590 600 KLEEILRGLT PRKNDIGDAM VFCLNNAEAA EEIVDCITES LSILKTPLPK KIARLYLVSD 610 620 630 640 650 660 VLYNSSAKVA NASYYRKFFE TKLCQIFSDL NATYRTIQGH LQSENFKQRV MTCFRAWEDW 670 680 690 700 710 720 AIYPEPFLIK LQNIFLGLVN IIEEKETEDV PDDLDGAPIE EELDGAPLED VDGIPIDATP 730 740 750 760 770 780 IDDLDGVPIK SLDDDLDGVP LDATEDSKKN EPIFKVAPSK WEAVDESELE AQAVTTSKWE 790 800 810 820 830 840 LFDQHEESEE EENQNQEEES EDEEDTQSSK SEEHHLYSNP IKEEMTESKF SKYSEMSEEK 850 860 870 880 890 900 RAKLREIELK VMKFQDELES GKRPKKPGQS FQEQVEHYRD KLLQREKEKE LERERERDKK 910 920 930 940 950 960 DKEKLESRSK DKKEKDECTP TRKERKRRHS TSPSPSRSSS GRRVKSPSPK SERSERSERS 970 980 990 1000 1010 1020 HKESSRSRSS HKDSPRDVSK KAKRSPSGSR TPKRSRRSRS RSPKKSGKKS RSQSRSPHRS HKKSKKNKH
	Isoform 2 [UniParc]. Checksum: 626B4D73CC24A992	FASTA	1,028	118,235
	10 20 30 40 50 60 MADKTPGGSQ KASSKTRSSD VHSSGSSDAH MDASGPSDSD MPSRTRPKSP RKHNYRNESA 70 80 90 100 110 120 RESLCDSPHQ NLSRPLLENK LKAFSIGKMS TAKRTLSKKE QEELKKKEDE KAAAEIYEEF 130 140 150 160 170 180 LAAFEGSDGN KVKTFVRGGV VNAAKEEHET DEKRGKIYKP SSRFADQKNP PNQSSNERPP 190 200 210 220 230 240 SLLVIETKKP PLKKGEKEKK KSNLELFKEE LKQIQEERDE RHKTKGRLSR FEPPQSDSDG 250 260 270 280 290 300 QRRSMDAPSR RNRSSVLDDY APGSHDVGDP STTNLYLGNI NPQMNEEMLC QEFGRFGPLA 310 320 330 340 350 360 SVKIMWPRTD EERARERNCG FVAFMNRRDA ERALKNLNGK MIMSFEMKLG WGKAVPIPPH 370 380 390 400 410 420 PIYIPPSMME HTLPPPPSGL PFNAQPRERL KNPNAPMLPP PKNKEDFEKT LSQAIVKVVI 430 440 450 460 470 480 PTERNLLALI HRMIEFVVRE GPMFEAMIMN REINNPMFRF LFENQTPAHV YYRWKLYSIL 490 500 510 520 530 540 QGDSPTKWRT EDFRMFKNGS FWRPPPLNPY LHGMSEEQET EAFVEEPSKK GALKEEQRDK 550 560 570 580 590 600 LEEILRGLTP RKNDIGDAMV FCLNNAEAAE EIVDCITESL SILKTPLPKK IARLYLVSDV 610 620 630 640 650 660 LYNSSAKVAN ASYYRKFFET KLCQIFSDLN ATYRTIQGHL QSENFKQRVM TCFRAWEDWA 670 680 690 700 710 720 IYPEPFLIKL QNIFLGLVNI IEEKETEDVP DDLDGAPIEE ELDGAPLEDV DGIPIDATPI 730 740 750 760 770 780 DDLDGVPIKS LDDDLDGVPL DATEDSKKNE PIFKVAPSKW EAVDESELEA QAVTTSKWEL 790 800 810 820 830 840 FDQHEESEEE ENQNQEEESE DEEDTQSSKS EEHHLYSNPI KEEMTESKFS KYSEMSEEKR 850 860 870 880 890 900 AKLREIELKV MKFQDELESG KRPKKPGQSF QEQVEHYRDK LLQREKEKEL ERERERDKKD 910 920 930 940 950 960 KEKLESRSKD KKEKDECTPT RKERKRRHST SPSPSRSSSG RRVKSPSPKS ERSERSERSH 970 980 990 1000 1010 1020 KESSRSRSSH KDSPRDVSKK AKRSPSGSRT PKRSRRSRSR SPKKSGKKSR SQSRSPHRSH KKSKKNKH
	Isoform 3 [UniParc]. Checksum: BA78B39559885CBC	FASTA	620	72,523
	10 20 30 40 50 60 MLLCYRHLKT KRILRRNLLA LIHRMIEFVV REGPMFEAMI MNREINNPMF RFLFENQTPA 70 80 90 100 110 120 HVYYRWKLYS ILQGDSPTKW RTEDFRMFKN GSFWRPPPLN PYLHGMSEEQ ETEAFVEEPS 130 140 150 160 170 180 KKGALKEEQR DKLEEILRGL TPRKNDIGDA MVFCLNNAEA AEEIVDCITE SLSILKTPLP 190 200 210 220 230 240 KKIARLYLVS DVLYNSSAKV ANASYYRKFF ETKLCQIFSD LNATYRTIQG HLQSENFKQR 250 260 270 280 290 300 VMTCFRAWED WAIYPEPFLI KLQNIFLGLV NIIEEKETED VPDDLDGAPI EEELDGAPLE 310 320 330 340 350 360 DVDGIPIDAT PIDDLDGVPI KSLDDDLDGV PLDATEDSKK NEPIFKVAPS KWEAVDESEL 370 380 390 400 410 420 EAQAVTTSKW ELFDQHEESE EEENQNQEEE SEDEEDTQSS KSEEHHLYSN PIKEEMTESK 430 440 450 460 470 480 FSKYSEMSEE KRAKLREIEL KVMKFQDELE SGKRPKKPGQ SFQEQVEHYR DKLLQREKEK 490 500 510 520 530 540 ELERERERDK KDKEKLESRS KDKKEKDECT PTRKERKRRH STSPSPSRSS SGRRVKSPSP 550 560 570 580 590 600 KSERSERSER SHKESSRSRS SHKDSPRDVS KKAKRSPSGS RTPKRSRRSR SRSPKKSGKK 610 620 SRSQSRSPHR SHKKSKKNKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1029 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-189 (ISOFORMS 1/2). Tissue: Lung and Urinary bladder.
[3]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1029 (ISOFORM 1). Tissue: Brain.
[4]	"Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein." Will C.L., Urlaub H., Achsel T., Gentzel M., Wilm M., Luehrmann R. EMBO J. 21:4978-4988(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-811, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-788 AND SER-800, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-788 AND SER-800, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-760, MASS SPECTROMETRY.
[9]	"Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways." Gilmore-Hebert M., Ramabhadran R., Stern D.F. Mol. Cancer Res. 8:1388-1398(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH ERBB4, SUBCELLULAR LOCATION.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485; SER-811; THR-931; SER-946 AND SER-948, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; SER-485; SER-788 AND SER-811, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC018450 Genomic DNA. No translation available. AC026304 Genomic DNA. No translation available. BC006474 mRNA. Translation: AAH06474.1. BC016323 mRNA. Translation: AAH16323.1. Sequence problems. BC105604 mRNA. Translation: AAI05605.1. Sequence problems. BC111692 mRNA. Translation: AAI11693.1. AB002330 mRNA. Translation: BAA20790.1. BK000564 mRNA. Translation: DAA00075.1.
IPI	IPI00143753. IPI00790699. IPI00829908.
RefSeq	NP_001073884.1. NM_001080415.1.
UniGene	Hs.596572.
3D structure databases
HSSP	HSSP built from PDB template 2CPD based on UniProtKB Q9NQ94.
ProteinModelPortal	O15042.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15042. 9 interactions.
PTM databases
PhosphoSite	O15042.
Proteomic databases
PaxDb	O15042.
PRIDE	O15042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319822; ENSP00000322376; ENSG00000163714. ENST00000397933; ENSP00000381027; ENSG00000163714. ENST00000473835; ENSP00000418563; ENSG00000163714.
GeneID	23350.
KEGG	hsa:23350.
UCSC	uc003evh.1. human. uc003evi.1. human. uc003evk.1. human.
Organism-specific databases
CTD	23350.
GeneCards	GC03P142720.
H-InvDB	HIX0003746.
HGNC	HGNC:30855. U2SURP.
HPA	HPA037545.
neXtProt	NX_O15042.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG307351.
HOGENOM	HOG000286037.
HOVERGEN	HBG093996.
InParanoid	O15042.
KO	K12842.
OMA	SILQANS.
OrthoDB	EOG47SSD9.
Gene expression databases
ArrayExpress	O15042.
Bgee	O15042.
Genevestigator	O15042.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR006569. CID_dom. IPR008942. ENTH_VHS. IPR013170. mRNA_splic_Cwf21. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR000061. Surp. [Graphical view]
Pfam	PF08312. cwf21. 1 hit. PF00076. RRM_1. 1 hit. PF01805. Surp. 1 hit. [Graphical view]
SMART	SM00582. RPR. 1 hit. SM00360. RRM. 1 hit. SM00648. SWAP. 1 hit. [Graphical view]
SUPFAM	SSF48464. ENTH_VHS. 1 hit. SSF109905. SSF109905. 1 hit.
PROSITE	PS51391. CID. 1 hit. PS50102. RRM. 1 hit. PS50128. SURP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	u2surp. human.
GenomeRNAi	23350.
NextBio	45335.
PMAP-CutDB	O15042.

Entry information

Entry name

SR140_HUMAN

Accession

Primary (citable) accession number: O15042
Secondary accession number(s): A0PJ60 , Q0D2M1, Q2NKQ7, Q9BR70

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.