O15016 (TRI66_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tripartite motif-containing protein 66

Gene names

Name:	TRIM66
Synonyms:	C11orf29, KIAA0298

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1216 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association By similarity.
Subunit structure	Can form homodimers and heterodimers. Interacts with CBX5, CBX1 and CBX3 via PxVxL motif By similarity.
Subcellular location	Nucleus. Note: Forms discrete foci within the centromeric chromocenter and surrounding nucleoplasm By similarity.
Sequence similarities	Contains 2 B box-type zinc fingers. Contains 1 bromo domain. Contains 1 PHD-type zinc finger.
Sequence caution	The sequence BAA20758.2 differs from that shown. Reason: Frameshift at position 797.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Bromodomain Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	aggresome Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O15016-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O15016-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1216	1216	Tripartite motif-containing protein 66		PRO_0000220375
Regions
	Domain	1056 – 1128	73	Bromo
	Zinc finger	1 – 46	46	B box-type 1; atypical
	Zinc finger	60 – 101	42	B box-type 2
	Zinc finger	970 – 1017	48	PHD-type
	Coiled coil	130 – 200	71	Potential
	Motif	860 – 864	5	PxVxL motif
	Compositional bias	384 – 571	188	Gln-rich
	Compositional bias	453 – 499	47	Pro-rich
Natural variations
	Alternative sequence	97 – 98	2	Missing in isoform 2.		VSP_038386

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 4. Checksum: 5E0F4953230617D1	FASTA	1,216	134,663
	10 20 30 40 50 60 MARNCSECKE KRAAHILCTY CNRWLCSSCT EEHRHSPVPG GPFFPRAQKG SPGVNGGPGD 70 80 90 100 110 120 FTLYCPLHTQ EVLKLFCETC DMLTCHSCLV VEHKEHRCRH VEEVLQNQRM LLEGVTTQVA 130 140 150 160 170 180 HKKSSLQTSA KQIEDRIFEV KHQHRKVENQ IKMAKMVLMN ELNKQANGLI EELEGITNER 190 200 210 220 230 240 KRKLEQQLQS IMVLNRQFEH VQNFINWAVC SKTSVPFLFS KELIVFQMQR LLETSCNTDP 250 260 270 280 290 300 GSPWSIRFTW EPNFWTKQLA SLGCITTEGG QMSRADAPAY GGLQGSSPFY QSHQSPVAQQ 310 320 330 340 350 360 EALSHPSHKF QSPAVCSSSV CCSHCSPVSP SLKGQVPPPS IHPAHSFRQP PEMVPQQLGS 370 380 390 400 410 420 LQCSALLPRE KELACSPHPP KLLQPWLETQ PPVEQESTSQ RLGQQLTSQP VCIVPPQDVQ 430 440 450 460 470 480 QGAHAQPTLQ TPSIQVQFGH HQKLKLSHFQ QQPQQQLPPP PPPLPHPPPP LPPPPQQPHP 490 500 510 520 530 540 PLPPSQHLAS SQHESPPGPA CSQNMDIMHH KFELEEMQKD LELLLQAQQP SLQLSQTKSP 550 560 570 580 590 600 QHLQQTIVGQ INYIVRQPAP VQSQSQEETL QATDEPPASQ GSKPALPLDK NTAAALPQAS 610 620 630 640 650 660 GEETPLSVPP VDSTIQHSSP NVVRKHSTSL SIMGFSNTLE MELSSTRLER PLEPQIQSVS 670 680 690 700 710 720 NLTAGAPQAV PSLLSAPPKM VSSLTSVQNQ AMPSLTTSHL QTVPSLVHST FQSMPNLISD 730 740 750 760 770 780 SPQAMASLAS DHPQAGPSLM SGHTQAVPSL ATCPLQSIPP VSDMQPETGS SSSSGRTSGS 790 800 810 820 830 840 LCPRDGADPS LENALCKVKL EEPINLSVKK PPLAPVVSTS TALQQYQNPK ECENFEQGAL 850 860 870 880 890 900 ELDAKENQSI RAFNSEHKIP YVRLERLKIC AASSGEMPVF KLKPQKNDQD GSFLLIIECG 910 920 930 940 950 960 TESSSMSIKV SQDRLSEATQ APGLEGRKVT VTSLAGQRPP EVEGTSPEEH RLIPRTPGAK 970 980 990 1000 1010 1020 KGPPAPIENE DFCAVCLNGG ELLCCDRCPK VFHLSCHVPA LLSFPGGEWV CTLCRSLTQP 1030 1040 1050 1060 1070 1080 EMEYDCENAC YNQPGMRASP GLSMYDQKKC EKLVLSLCCN NLSLPFHEPV SPLARHYYQI 1090 1100 1110 1120 1130 1140 IKRPMDLSII RRKLQKKDPA HYTTPEEVVS DVRLMFWNCA KFNYPDSEVA EAGRCLEVFF 1150 1160 1170 1180 1190 1200 EGWLKEIYPE KRFAQPRQED SDSEEVSSES GCSTPQGFPW PPYMQEGIQP KRRRRHMENE 1210 RAKRMSFRLA NSISQV
	Isoform 2 [UniParc]. Checksum: A3973C0EA2D47C3A	FASTA	1,214	134,404
	10 20 30 40 50 60 MARNCSECKE KRAAHILCTY CNRWLCSSCT EEHRHSPVPG GPFFPRAQKG SPGVNGGPGD 70 80 90 100 110 120 FTLYCPLHTQ EVLKLFCETC DMLTCHSCLV VEHKEHRHVE EVLQNQRMLL EGVTTQVAHK 130 140 150 160 170 180 KSSLQTSAKQ IEDRIFEVKH QHRKVENQIK MAKMVLMNEL NKQANGLIEE LEGITNERKR 190 200 210 220 230 240 KLEQQLQSIM VLNRQFEHVQ NFINWAVCSK TSVPFLFSKE LIVFQMQRLL ETSCNTDPGS 250 260 270 280 290 300 PWSIRFTWEP NFWTKQLASL GCITTEGGQM SRADAPAYGG LQGSSPFYQS HQSPVAQQEA 310 320 330 340 350 360 LSHPSHKFQS PAVCSSSVCC SHCSPVSPSL KGQVPPPSIH PAHSFRQPPE MVPQQLGSLQ 370 380 390 400 410 420 CSALLPREKE LACSPHPPKL LQPWLETQPP VEQESTSQRL GQQLTSQPVC IVPPQDVQQG 430 440 450 460 470 480 AHAQPTLQTP SIQVQFGHHQ KLKLSHFQQQ PQQQLPPPPP PLPHPPPPLP PPPQQPHPPL 490 500 510 520 530 540 PPSQHLASSQ HESPPGPACS QNMDIMHHKF ELEEMQKDLE LLLQAQQPSL QLSQTKSPQH 550 560 570 580 590 600 LQQTIVGQIN YIVRQPAPVQ SQSQEETLQA TDEPPASQGS KPALPLDKNT AAALPQASGE 610 620 630 640 650 660 ETPLSVPPVD STIQHSSPNV VRKHSTSLSI MGFSNTLEME LSSTRLERPL EPQIQSVSNL 670 680 690 700 710 720 TAGAPQAVPS LLSAPPKMVS SLTSVQNQAM PSLTTSHLQT VPSLVHSTFQ SMPNLISDSP 730 740 750 760 770 780 QAMASLASDH PQAGPSLMSG HTQAVPSLAT CPLQSIPPVS DMQPETGSSS SSGRTSGSLC 790 800 810 820 830 840 PRDGADPSLE NALCKVKLEE PINLSVKKPP LAPVVSTSTA LQQYQNPKEC ENFEQGALEL 850 860 870 880 890 900 DAKENQSIRA FNSEHKIPYV RLERLKICAA SSGEMPVFKL KPQKNDQDGS FLLIIECGTE 910 920 930 940 950 960 SSSMSIKVSQ DRLSEATQAP GLEGRKVTVT SLAGQRPPEV EGTSPEEHRL IPRTPGAKKG 970 980 990 1000 1010 1020 PPAPIENEDF CAVCLNGGEL LCCDRCPKVF HLSCHVPALL SFPGGEWVCT LCRSLTQPEM 1030 1040 1050 1060 1070 1080 EYDCENACYN QPGMRASPGL SMYDQKKCEK LVLSLCCNNL SLPFHEPVSP LARHYYQIIK 1090 1100 1110 1120 1130 1140 RPMDLSIIRR KLQKKDPAHY TTPEEVVSDV RLMFWNCAKF NYPDSEVAEA GRCLEVFFEG 1150 1160 1170 1180 1190 1200 WLKEIYPEKR FAQPRQEDSD SEEVSSESGC STPQGFPWPP YMQEGIQPKR RRRHMENERA 1210 KRMSFRLANS ISQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7." Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R. Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1).
[2]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-868 (ISOFORM 2). Tissue: Brain.
[3]	Nagase T., Ishikawa K., Seki N., Nakajima D., Ohira M., Miyajima N., Kotani H., Nomura N., Ohara O. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ400879 Genomic DNA. Translation: CAC35389.1. AB002296 mRNA. Translation: BAA20758.2. Frameshift. AC091053 Genomic DNA. No translation available. AC104360 Genomic DNA. No translation available.
IPI	IPI00004420. IPI00941834.
RefSeq	NP_055633.1. NM_014818.1.
UniGene	Hs.130836.
3D structure databases
ProteinModelPortal	O15016.
SMR	O15016. Positions 59-103, 969-1149.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000299550.
PTM databases
PhosphoSite	O15016.
Proteomic databases
PaxDb	O15016.
PRIDE	O15016.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299550; ENSP00000299550; ENSG00000166436.
GeneID	9866.
KEGG	hsa:9866.
UCSC	uc010rbo.2. human.
Organism-specific databases
CTD	9866.
GeneCards	GC11M008595.
H-InvDB	HIX0201585.
HGNC	HGNC:29005. TRIM66.
HPA	HPA027420.
MIM	612000. gene.
neXtProt	NX_O15016.
PharmGKB	PA134954583.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5076.
HOGENOM	HOG000074104.
HOVERGEN	HBG057849.
KO	K12032.
Gene expression databases
ArrayExpress	O15016.
Bgee	O15016.
CleanEx	HS_TRIM66.
Genevestigator	O15016.
GermOnline	ENSG00000166436. Homo sapiens.
Family and domain databases
Gene3D	1.20.920.10. 2 hits. 3.30.40.10. 1 hit.
InterPro	IPR003649. Bbox_C. IPR001487. Bromodomain. IPR019786. Zinc_finger_PHD-type_CS. IPR000315. Znf_B-box. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF00439. Bromodomain. 1 hit. PF00628. PHD. 1 hit. PF00643. zf-B_box. 1 hit. [Graphical view]
SMART	SM00502. BBC. 1 hit. SM00297. BROMO. 1 hit. SM00249. PHD. 2 hits. [Graphical view]
SUPFAM	SSF47370. Bromodomain. 1 hit. SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS00633. BROMODOMAIN_1. False negative. PS50014. BROMODOMAIN_2. 1 hit. PS50119. ZF_BBOX. 2 hits. PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9866.
NextBio	37189.
SOURCE	Search...

Entry information

Entry name

TRI66_HUMAN

Accession

Primary (citable) accession number: O15016
Secondary accession number(s): Q9BQQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 100 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.