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Protein

Tripartite motif-containing protein 66

Gene

TRIM66

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 4646B box-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri60 – 10142B box-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri970 – 101748PHD-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 66
Gene namesi
Name:TRIM66
Synonyms:C11orf29, KIAA0298
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:29005. TRIM66.

Subcellular locationi

  • Nucleus

  • Note: Forms discrete foci within the centromeric chromocenter and surrounding nucleoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134954583.

Polymorphism and mutation databases

BioMutaiTRIM66.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12161216Tripartite motif-containing protein 66PRO_0000220375Add
BLAST

Proteomic databases

PaxDbiO15016.
PRIDEiO15016.

PTM databases

PhosphoSiteiO15016.

Expressioni

Gene expression databases

BgeeiO15016.
CleanExiHS_TRIM66.
ExpressionAtlasiO15016. baseline and differential.
GenevisibleiO15016. HS.

Organism-specific databases

HPAiHPA027420.

Interactioni

Subunit structurei

Can form homodimers and heterodimers. Interacts with CBX5, CBX1 and CBX3 via PxVxL motif (By similarity).By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000384876.

Structurei

3D structure databases

ProteinModelPortaliO15016.
SMRiO15016. Positions 3-37, 59-103, 969-1181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1056 – 112873BromoPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili130 – 20071Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi860 – 8645PxVxL motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi384 – 571188Gln-richAdd
BLAST
Compositional biasi453 – 49947Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 4646B box-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri60 – 10142B box-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri970 – 101748PHD-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Bromodomain, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5076.
GeneTreeiENSGT00530000062982.
HOGENOMiHOG000074104.
HOVERGENiHBG057849.
InParanoidiO15016.
KOiK12032.
PhylomeDBiO15016.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15016-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARNCSECKE KRAAHILCTY CNRWLCSSCT EEHRHSPVPG GPFFPRAQKG
60 70 80 90 100
SPGVNGGPGD FTLYCPLHTQ EVLKLFCETC DMLTCHSCLV VEHKEHRCRH
110 120 130 140 150
VEEVLQNQRM LLEGVTTQVA HKKSSLQTSA KQIEDRIFEV KHQHRKVENQ
160 170 180 190 200
IKMAKMVLMN ELNKQANGLI EELEGITNER KRKLEQQLQS IMVLNRQFEH
210 220 230 240 250
VQNFINWAVC SKTSVPFLFS KELIVFQMQR LLETSCNTDP GSPWSIRFTW
260 270 280 290 300
EPNFWTKQLA SLGCITTEGG QMSRADAPAY GGLQGSSPFY QSHQSPVAQQ
310 320 330 340 350
EALSHPSHKF QSPAVCSSSV CCSHCSPVSP SLKGQVPPPS IHPAHSFRQP
360 370 380 390 400
PEMVPQQLGS LQCSALLPRE KELACSPHPP KLLQPWLETQ PPVEQESTSQ
410 420 430 440 450
RLGQQLTSQP VCIVPPQDVQ QGAHAQPTLQ TPSIQVQFGH HQKLKLSHFQ
460 470 480 490 500
QQPQQQLPPP PPPLPHPPPP LPPPPQQPHP PLPPSQHLAS SQHESPPGPA
510 520 530 540 550
CSQNMDIMHH KFELEEMQKD LELLLQAQQP SLQLSQTKSP QHLQQTIVGQ
560 570 580 590 600
INYIVRQPAP VQSQSQEETL QATDEPPASQ GSKPALPLDK NTAAALPQAS
610 620 630 640 650
GEETPLSVPP VDSTIQHSSP NVVRKHSTSL SIMGFSNTLE MELSSTRLER
660 670 680 690 700
PLEPQIQSVS NLTAGAPQAV PSLLSAPPKM VSSLTSVQNQ AMPSLTTSHL
710 720 730 740 750
QTVPSLVHST FQSMPNLISD SPQAMASLAS DHPQAGPSLM SGHTQAVPSL
760 770 780 790 800
ATCPLQSIPP VSDMQPETGS SSSSGRTSGS LCPRDGADPS LENALCKVKL
810 820 830 840 850
EEPINLSVKK PPLAPVVSTS TALQQYQNPK ECENFEQGAL ELDAKENQSI
860 870 880 890 900
RAFNSEHKIP YVRLERLKIC AASSGEMPVF KLKPQKNDQD GSFLLIIECG
910 920 930 940 950
TESSSMSIKV SQDRLSEATQ APGLEGRKVT VTSLAGQRPP EVEGTSPEEH
960 970 980 990 1000
RLIPRTPGAK KGPPAPIENE DFCAVCLNGG ELLCCDRCPK VFHLSCHVPA
1010 1020 1030 1040 1050
LLSFPGGEWV CTLCRSLTQP EMEYDCENAC YNQPGMRASP GLSMYDQKKC
1060 1070 1080 1090 1100
EKLVLSLCCN NLSLPFHEPV SPLARHYYQI IKRPMDLSII RRKLQKKDPA
1110 1120 1130 1140 1150
HYTTPEEVVS DVRLMFWNCA KFNYPDSEVA EAGRCLEVFF EGWLKEIYPE
1160 1170 1180 1190 1200
KRFAQPRQED SDSEEVSSES GCSTPQGFPW PPYMQEGIQP KRRRRHMENE
1210
RAKRMSFRLA NSISQV
Length:1,216
Mass (Da):134,663
Last modified:November 24, 2009 - v4
Checksum:i5E0F4953230617D1
GO
Isoform 2 (identifier: O15016-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: Missing.

Show »
Length:1,214
Mass (Da):134,404
Checksum:iA3973C0EA2D47C3A
GO

Sequence cautioni

The sequence BAA20758.2 differs from that shown. Reason: Frameshift at position 797. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei97 – 982Missing in isoform 2. 1 PublicationVSP_038386

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ400879 Genomic DNA. Translation: CAC35389.1.
AB002296 mRNA. Translation: BAA20758.2. Frameshift.
AC091053 Genomic DNA. No translation available.
AC104360 Genomic DNA. No translation available.
RefSeqiNP_055633.1. NM_014818.1. [O15016-1]
UniGeneiHs.130836.
Hs.716965.

Genome annotation databases

EnsembliENST00000299550; ENSP00000299550; ENSG00000166436. [O15016-1]
GeneIDi9866.
KEGGihsa:9866.
UCSCiuc010rbo.2. human. [O15016-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ400879 Genomic DNA. Translation: CAC35389.1.
AB002296 mRNA. Translation: BAA20758.2. Frameshift.
AC091053 Genomic DNA. No translation available.
AC104360 Genomic DNA. No translation available.
RefSeqiNP_055633.1. NM_014818.1. [O15016-1]
UniGeneiHs.130836.
Hs.716965.

3D structure databases

ProteinModelPortaliO15016.
SMRiO15016. Positions 3-37, 59-103, 969-1181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000384876.

PTM databases

PhosphoSiteiO15016.

Polymorphism and mutation databases

BioMutaiTRIM66.

Proteomic databases

PaxDbiO15016.
PRIDEiO15016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299550; ENSP00000299550; ENSG00000166436. [O15016-1]
GeneIDi9866.
KEGGihsa:9866.
UCSCiuc010rbo.2. human. [O15016-1]

Organism-specific databases

CTDi9866.
GeneCardsiGC11M008595.
H-InvDBHIX0201585.
HGNCiHGNC:29005. TRIM66.
HPAiHPA027420.
MIMi612000. gene.
neXtProtiNX_O15016.
PharmGKBiPA134954583.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5076.
GeneTreeiENSGT00530000062982.
HOGENOMiHOG000074104.
HOVERGENiHBG057849.
InParanoidiO15016.
KOiK12032.
PhylomeDBiO15016.

Miscellaneous databases

ChiTaRSiTRIM66. human.
GenomeRNAii9866.
NextBioi37189.
PROiO15016.
SOURCEiSearch...

Gene expression databases

BgeeiO15016.
CleanExiHS_TRIM66.
ExpressionAtlasiO15016. baseline and differential.
GenevisibleiO15016. HS.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7."
    Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R.
    Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1).
  2. "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-868 (ISOFORM 2).
    Tissue: Brain.
  3. Nagase T., Ishikawa K., Seki N., Nakajima D., Ohira M., Miyajima N., Kotani H., Nomura N., Ohara O.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTRI66_HUMAN
AccessioniPrimary (citable) accession number: O15016
Secondary accession number(s): Q9BQQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 24, 2009
Last modified: June 24, 2015
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.