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Protein

TATA-binding protein-associated factor 172

Gene

BTAF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1291 – 12988ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • negative regulation of chromatin binding Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-binding protein-associated factor 172 (EC:3.6.4.-)
Alternative name(s):
ATP-dependent helicase BTAF1
B-TFIID transcription factor-associated 170 kDa subunit
TAF(II)170
TBP-associated factor 172
Short name:
TAF-172
Gene namesi
Name:BTAF1
Synonyms:TAF172
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17307. BTAF1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25437.

Polymorphism and mutation databases

BioMutaiBTAF1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18491849TATA-binding protein-associated factor 172PRO_0000074311Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei91 – 911PhosphoserineCombined sources
Modified residuei95 – 951PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14981.
PaxDbiO14981.
PeptideAtlasiO14981.
PRIDEiO14981.

PTM databases

iPTMnetiO14981.
PhosphoSiteiO14981.

Expressioni

Gene expression databases

BgeeiENSG00000095564.
CleanExiHS_BTAF1.
ExpressionAtlasiO14981. baseline and differential.
GenevisibleiO14981. HS.

Organism-specific databases

HPAiHPA042274.
HPA070682.

Interactioni

Subunit structurei

Associates with TBP to form B-TFIID. Binds DRAP1.

Protein-protein interaction databases

BioGridi114506. 41 interactions.
IntActiO14981. 17 interactions.
MINTiMINT-2795412.
STRINGi9606.ENSP00000265990.

Structurei

3D structure databases

ProteinModelPortaliO14981.
SMRiO14981. Positions 282-541, 1259-1800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati385 – 42238HEAT 1Add
BLAST
Repeati426 – 46338HEAT 2Add
BLAST
Repeati513 – 55038HEAT 3Add
BLAST
Repeati554 – 59643HEAT 4Add
BLAST
Repeati818 – 85538HEAT 5Add
BLAST
Repeati872 – 91039HEAT 6Add
BLAST
Repeati1102 – 113938HEAT 7Add
BLAST
Repeati1182 – 121938HEAT 8Add
BLAST
Domaini1278 – 1453176Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1636 – 1790155Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 20717Nuclear localization signalSequence analysisAdd
BLAST
Motifi1404 – 14074DEGH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 8 HEAT repeats.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0387. Eukaryota.
ENOG410XP4Z. LUCA.
GeneTreeiENSGT00630000089754.
HOGENOMiHOG000210415.
HOVERGENiHBG017883.
InParanoidiO14981.
KOiK15192.
OMAiREEDMVD.
PhylomeDBiO14981.
TreeFamiTF300546.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
3.40.50.300. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022707. DUF3535.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF12054. DUF3535. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14981-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVSRLDRLF ILLDTGTTPV TRKAAAQQLG EVVKLHPHEL NNLLSKVLIY
60 70 80 90 100
LRSANWDTRI AAGQAVEAIV KNVPEWNPVP RTRQEPTSES SMEDSPTTER
110 120 130 140 150
LNFDRFDICR LLQHGASLLG SAGAEFEVQD EKSGEVDPKE RIARQRKLLQ
160 170 180 190 200
KKLGLNMGEA IGMSTEELFN DEDLDYTPTS ASFVNKQPTL QAAELIDSEF
210 220 230 240 250
RAGMSNRQKN KAKRMAKLFA KQRSRDAVET NEKSNDSTDG EPEEKRRKIA
260 270 280 290 300
NVVINQSAND SKVLIDNIPD SSSLIEETNE WPLESFCEEL CNDLFNPSWE
310 320 330 340 350
VRHGAGTGLR EILKAHGKSG GKMGDSTLEE MIQQHQEWLE DLVIRLLCVF
360 370 380 390 400
ALDRFGDFVS DEVVAPVRET CAQTLGVVLK HMNETGVHKT VDVLLKLLTQ
410 420 430 440 450
EQWEVRHGGL LGIKYALAVR QDVINTLLPK VLTRIIEGLQ DLDDDVRAVA
460 470 480 490 500
AASLVPVVES LVYLQTQKVP FIINTLWDAL LELDDLTAST NSIMTLLSSL
510 520 530 540 550
LTYPQVQQCS IQQSLTVLVP RVWPFLHHTI SSVRRAALET LFTLLSTQDQ
560 570 580 590 600
NSSSWLIPIL PDMLRHIFQF CVLESSQEIL DLIHKVWMEL LSKASVQYVV
610 620 630 640 650
AAACPWMGAW LCLMMQPSHL PIDLNMLLEV KARAKEKTGG KVRQGQSQNK
660 670 680 690 700
EVLQEYIAGA DTIMEDPATR DFVVMRARMM AAKLLGALCC CICDPGVNVV
710 720 730 740 750
TQEIKPAESL GQLLLFHLNS KSALQRISVA LVICEWAALQ KECKAVTLAV
760 770 780 790 800
QPRLLDILSE HLYYDEIAVP FTRMQNECKQ LISSLADVHI EVGNRVNNNV
810 820 830 840 850
LTIDQASDLV TTVFNEATSS FDLNPQVLQQ LDSKRQQVQM TVTETNQEWQ
860 870 880 890 900
VLQLRVHTFA ACAVVSLQQL PEKLNPIIKP LMETIKKEEN TLVQNYAAQC
910 920 930 940 950
IAKLLQQCTT RTPCPNSKII KNLCSSLCVD PYLTPCVTCP VPTQSGQENS
960 970 980 990 1000
KGSTSEKDGM HHTVTKHRGI ITLYRHQKAA FAITSRRGPT PKAVKAQIAD
1010 1020 1030 1040 1050
LPAGSSGNIL VELDEAQKPY LVQRRGAEFA LTTIVKHFGG EMAVKLPHLW
1060 1070 1080 1090 1100
DAMVGPLRNT IDINNFDGKS LLDKGDSPAQ ELVNSLQVFE TAAASMDSEL
1110 1120 1130 1140 1150
HPLLVQHLPH LYMCLQYPST AVRHMAARCV GVMSKIATME TMNIFLEKVL
1160 1170 1180 1190 1200
PWLGAIDDSV KQEGAIEALA CVMEQLDVGI VPYIVLLVVP VLGRMSDQTD
1210 1220 1230 1240 1250
SVRFMATQCF ATLIRLMPLE AGIPDPPNMS AELIQLKAKE RHFLEQLLDG
1260 1270 1280 1290 1300
KKLENYKIPV PINAELRKYQ QDGVNWLAFL NKYKLHGILC DDMGLGKTLQ
1310 1320 1330 1340 1350
SICILAGDHC HRAQEYARSK LAECMPLPSL VVCPPTLTGH WVDEVGKFCS
1360 1370 1380 1390 1400
REYLNPLHYT GPPTERIRLQ HQVKRHNLIV ASYDVVRNDI DFFRNIKFNY
1410 1420 1430 1440 1450
CILDEGHVIK NGKTKLSKAV KQLTANYRII LSGTPIQNNV LELWSLFDFL
1460 1470 1480 1490 1500
MPGFLGTERQ FAARYGKPIL ASRDARSSSR EQEAGVLAMD ALHRQVLPFL
1510 1520 1530 1540 1550
LRRMKEDVLQ DLPPKIIQDY YCTLSPLQVQ LYEDFAKSRA KCDVDETVSS
1560 1570 1580 1590 1600
ATLSEETEKP KLKATGHVFQ ALQYLRKLCN HPALVLTPQH PEFKTTAEKL
1610 1620 1630 1640 1650
AVQNSSLHDI QHAPKLSALK QLLLDCGLGN GSTSESGTES VVAQHRILIF
1660 1670 1680 1690 1700
CQLKSMLDIV EHDLLKPHLP SVTYLRLDGS IPPGQRHSIV SRFNNDPSID
1710 1720 1730 1740 1750
VLLLTTHVGG LGLNLTGADT VVFVEHDWNP MRDLQAMDRA HRIGQKRVVN
1760 1770 1780 1790 1800
VYRLITRGTL EEKIMGLQKF KMNIANTVIS QENSSLQSMG TDQLLDLFTL
1810 1820 1830 1840
DKDGKAEKAD TSTSGKASMK SILENLSDLW DQEQYDSEYS LENFMHSLK
Length:1,849
Mass (Da):206,887
Last modified:August 1, 1998 - v2
Checksum:iFE3675B75A44F113
GO
Isoform 2 (identifier: O14981-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1172: Missing.

Note: No experimental confirmation available.
Show »
Length:677
Mass (Da):76,419
Checksum:iDA3558BDAEF2B0D0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11721172Missing in isoform 2. 1 PublicationVSP_056510Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001017 mRNA. Translation: CAA04475.1.
AF038362 mRNA. Translation: AAC04573.1.
AK303554 mRNA. Translation: BAG64578.1.
AL359198 Genomic DNA. No translation available.
AL365398 Genomic DNA. No translation available.
AF166118 Genomic DNA. Translation: AAF37803.1.
CCDSiCCDS7419.1. [O14981-1]
RefSeqiNP_003963.1. NM_003972.2. [O14981-1]
UniGeneiHs.500526.

Genome annotation databases

EnsembliENST00000265990; ENSP00000265990; ENSG00000095564. [O14981-1]
GeneIDi9044.
KEGGihsa:9044.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001017 mRNA. Translation: CAA04475.1.
AF038362 mRNA. Translation: AAC04573.1.
AK303554 mRNA. Translation: BAG64578.1.
AL359198 Genomic DNA. No translation available.
AL365398 Genomic DNA. No translation available.
AF166118 Genomic DNA. Translation: AAF37803.1.
CCDSiCCDS7419.1. [O14981-1]
RefSeqiNP_003963.1. NM_003972.2. [O14981-1]
UniGeneiHs.500526.

3D structure databases

ProteinModelPortaliO14981.
SMRiO14981. Positions 282-541, 1259-1800.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114506. 41 interactions.
IntActiO14981. 17 interactions.
MINTiMINT-2795412.
STRINGi9606.ENSP00000265990.

PTM databases

iPTMnetiO14981.
PhosphoSiteiO14981.

Polymorphism and mutation databases

BioMutaiBTAF1.

Proteomic databases

EPDiO14981.
PaxDbiO14981.
PeptideAtlasiO14981.
PRIDEiO14981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265990; ENSP00000265990; ENSG00000095564. [O14981-1]
GeneIDi9044.
KEGGihsa:9044.

Organism-specific databases

CTDi9044.
GeneCardsiBTAF1.
HGNCiHGNC:17307. BTAF1.
HPAiHPA042274.
HPA070682.
MIMi605191. gene.
neXtProtiNX_O14981.
PharmGKBiPA25437.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0387. Eukaryota.
ENOG410XP4Z. LUCA.
GeneTreeiENSGT00630000089754.
HOGENOMiHOG000210415.
HOVERGENiHBG017883.
InParanoidiO14981.
KOiK15192.
OMAiREEDMVD.
PhylomeDBiO14981.
TreeFamiTF300546.

Miscellaneous databases

ChiTaRSiBTAF1. human.
GeneWikiiBTAF1.
GenomeRNAii9044.
PROiO14981.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000095564.
CleanExiHS_BTAF1.
ExpressionAtlasiO14981. baseline and differential.
GenevisibleiO14981. HS.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
3.40.50.300. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022707. DUF3535.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF12054. DUF3535. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTAF1_HUMAN
AccessioniPrimary (citable) accession number: O14981
Secondary accession number(s): B4E0W6, O43578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.