Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cyclin-G-associated kinase

Gene

GAK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin binding Source: ParkinsonsUK-UCL
  • protein serine/threonine kinase activity Source: ProtInc

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • clathrin coat disassembly Source: ParkinsonsUK-UCL
  • clathrin-dependent endocytosis Source: ParkinsonsUK-UCL
  • endoplasmic reticulum organization Source: ParkinsonsUK-UCL
  • establishment of protein localization to Golgi Source: ParkinsonsUK-UCL
  • establishment of protein localization to plasma membrane Source: ParkinsonsUK-UCL
  • Golgi organization Source: ParkinsonsUK-UCL
  • Golgi to lysosome transport Source: ParkinsonsUK-UCL
  • negative regulation of neuron projection development Source: ParkinsonsUK-UCL
  • receptor-mediated endocytosis Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS11332-MONOMER.
ReactomeiR-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-8856828. Clathrin-mediated endocytosis.
SignaLinkiO14976.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:GAK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:4113. GAK.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • focal adhesion Source: UniProtKB-SubCell
  • Golgi apparatus Source: ParkinsonsUK-UCL
  • intracellular membrane-bounded organelle Source: HPA
  • membrane Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • vesicle Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2580.
OpenTargetsiENSG00000178950.
PharmGKBiPA28528.

Chemistry databases

ChEMBLiCHEMBL4355.
GuidetoPHARMACOLOGYi2027.

Polymorphism and mutation databases

BioMutaiGAK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000859582 – 1311Cyclin-G-associated kinaseAdd BLAST1310

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineBy similarity1
Modified residuei16PhosphoserineCombined sources1
Modified residuei456PhosphoserineCombined sources1
Modified residuei770PhosphoserineCombined sources1
Modified residuei776PhosphothreonineCombined sources1
Modified residuei783PhosphoserineCombined sources1
Modified residuei794PhosphothreonineCombined sources1
Modified residuei811PhosphoserineBy similarity1
Modified residuei826PhosphoserineCombined sources1
Modified residuei829PhosphoserineCombined sources1
Modified residuei834PhosphoserineCombined sources1
Modified residuei939PhosphoserineCombined sources1
Modified residuei1096PhosphoserineCombined sources1
Modified residuei1123Omega-N-methylarginineBy similarity1
Modified residuei1176PhosphoserineCombined sources1
Modified residuei1185PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO14976.
MaxQBiO14976.
PaxDbiO14976.
PeptideAtlasiO14976.
PRIDEiO14976.

PTM databases

iPTMnetiO14976.
PhosphoSitePlusiO14976.

Expressioni

Tissue specificityi

Ubiquitous. Highest in testis.

Gene expression databases

BgeeiENSG00000178950.
CleanExiHS_GAK.
ExpressionAtlasiO14976. baseline and differential.
GenevisibleiO14976. HS.

Organism-specific databases

HPAiHPA027405.
HPA027459.
HPA027463.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ATP13A2Q9NQ112EBI-714707,EBI-6308763
HSPA8P111425EBI-714707,EBI-351896
LRRK2Q5S00710EBI-714707,EBI-5323863

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108853. 92 interactors.
IntActiO14976. 80 interactors.
MINTiMINT-5000432.
STRINGi9606.ENSP00000314499.

Chemistry databases

BindingDBiO14976.

Structurei

Secondary structure

11311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni27 – 30Combined sources4
Beta strandi32 – 35Combined sources4
Beta strandi38 – 47Combined sources10
Beta strandi50 – 59Combined sources10
Turni60 – 62Combined sources3
Beta strandi65 – 75Combined sources11
Helixi76 – 92Combined sources17
Beta strandi101 – 107Combined sources7
Turni109 – 111Combined sources3
Beta strandi115 – 124Combined sources10
Beta strandi127 – 129Combined sources3
Helixi130 – 138Combined sources9
Helixi145 – 163Combined sources19
Beta strandi165 – 167Combined sources3
Helixi176 – 178Combined sources3
Beta strandi179 – 181Combined sources3
Beta strandi187 – 189Combined sources3
Helixi208 – 221Combined sources14
Helixi224 – 226Combined sources3
Helixi229 – 232Combined sources4
Helixi242 – 258Combined sources17
Turni262 – 265Combined sources4
Helixi268 – 272Combined sources5
Helixi286 – 288Combined sources3
Helixi289 – 295Combined sources7
Helixi300 – 302Combined sources3
Helixi306 – 319Combined sources14
Helixi329 – 332Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C57X-ray2.55A/B14-351[»]
4C58X-ray2.16A27-351[»]
4C59X-ray2.80A14-351[»]
4O38X-ray2.10A/B12-347[»]
4Y8DX-ray2.10A/B14-351[»]
ProteinModelPortaliO14976.
SMRiO14976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 314Protein kinasePROSITE-ProRule annotationAdd BLAST275
Domaini399 – 566Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST168
Domaini572 – 710C2 tensin-typePROSITE-ProRule annotationAdd BLAST139
Domaini1247 – 1311JPROSITE-ProRule annotationAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi347 – 350Poly-Pro4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0431. Eukaryota.
KOG1989. Eukaryota.
KOG2283. Eukaryota.
ENOG410Y515. LUCA.
GeneTreeiENSGT00760000119113.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiO14976.
KOiK08855.
OMAiKKNNIRA.
OrthoDBiEOG091G06HJ.
PhylomeDBiO14976.
TreeFamiTF105165.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM01326. PTEN_C2. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14976-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLQSALDF LAGPGSLGGA SGRDQSDFVG QTVELGELRL RVRRVLAEGG
60 70 80 90 100
FAFVYEAQDV GSGREYALKR LLSNEEEKNR AIIQEVCFMK KLSGHPNIVQ
110 120 130 140 150
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKKMESR GPLSCDTVLK
160 170 180 190 200
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH
210 220 230 240 250
YPDYSWSAQR RALVEEEITR NTTPMYRTPE IIDLYSNFPI GEKQDIWALG
260 270 280 290 300
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPP HDTQYTVFHS LIRAMLQVNP
310 320 330 340 350
EERLSIAEVV HQLQEIAAAR NVNPKSPITE LLEQNGGYGS ATLSRGPPPP
360 370 380 390 400
VGPAGSGYSG GLALAEYDQP YGGFLDILRG GTERLFTNLK DTSSKVIQSV
410 420 430 440 450
ANYAKGDLDI SYITSRIAVM SFPAEGVESA LKNNIEDVRL FLDSKHPGHY
460 470 480 490 500
AVYNLSPRTY RPSRFHNRVS ECGWAARRAP HLHTLYNICR NMHAWLRQDH
510 520 530 540 550
KNVCVVHCMD GRAASAVAVC SFLCFCRLFS TAEAAVYMFS MKRCPPGIWP
560 570 580 590 600
SHKRYIEYMC DMVAEEPITP HSKPILVRAV VMTPVPLFSK QRSGCRPFCE
610 620 630 640 650
VYVGDERVAS TSQEYDKMRD FKIEDGKAVI PLGVTVQGDV LIVIYHARST
660 670 680 690 700
LGGRLQAKMA SMKMFQIQFH TGFVPRNATT VKFAKYDLDA CDIQEKYPDL
710 720 730 740 750
FQVNLEVEVE PRDRPSREAP PWENSSMRGL NPKILFSSRE EQQDILSKFG
760 770 780 790 800
KPELPRQPGS TAQYDAGAGS PEAEPTDSDS PPSSSADASR FLHTLDWQEE
810 820 830 840 850
KEAETGAENA SSKESESALM EDRDESEVSD EGGSPISSEG QEPRADPEPP
860 870 880 890 900
GLAAGLVQQD LVFEVETPAV LPEPVPQEDG VDLLGLHSEV GAGPAVPPQA
910 920 930 940 950
CKAPSSNTDL LSCLLGPPEA ASQGPPEDLL SEDPLLLASP APPLSVQSTP
960 970 980 990 1000
RGGPPAAADP FGPLLPSSGN NSQPCSNPDL FGEFLNSDSV TVPPSFPSAH
1010 1020 1030 1040 1050
SAPPPSCSAD FLHLGDLPGE PSKMTASSSN PDLLGGWAAW TETAASAVAP
1060 1070 1080 1090 1100
TPATEGPLFS PGGQPAPCGS QASWTKSQNP DPFADLGDLS SGLQGSPAGF
1110 1120 1130 1140 1150
PPGGFIPKTA TTPKGSSSWQ TSRPPAQGAS WPPQAKPPPK ACTQPRPNYA
1160 1170 1180 1190 1200
SNFSVIGARE ERGVRAPSFA QKPKVSENDF EDLLSNQGFS SRSDKKGPKT
1210 1220 1230 1240 1250
IAEMRKQDLA KDTDPLKLKL LDWIEGKERN IRALLSTLHT VLWDGESRWT
1260 1270 1280 1290 1300
PVGMADLVAP EQVKKHYRRA VLAVHPDKAA GQPYEQHAKM IFMELNDAWS
1310
EFENQGSRPL F
Length:1,311
Mass (Da):143,191
Last modified:August 29, 2001 - v2
Checksum:i0ACE45DF57A5F981
GO
Isoform 2 (identifier: O14976-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     49-127: Missing.

Note: No experimental confirmation available.
Show »
Length:1,232
Mass (Da):134,464
Checksum:i483EE5F6B8416FDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti607R → C in AAH85005 (PubMed:15489334).Curated1
Sequence conflicti1113P → A in BAA22623 (PubMed:9299234).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040505144S → L.1 PublicationCorresponds to variant rs768962219dbSNPEnsembl.1
Natural variantiVAR_040506580V → M.1 PublicationCorresponds to variant rs34255232dbSNPEnsembl.1
Natural variantiVAR_040507787D → Y.1 PublicationCorresponds to variant rs34585705dbSNPEnsembl.1
Natural variantiVAR_040508877Q → R.1 PublicationCorresponds to variant rs149842313dbSNPEnsembl.1
Natural variantiVAR_040509962G → D in a lung neuroendocrine carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_0405101051T → M.1 PublicationCorresponds to variant rs35227944dbSNPEnsembl.1
Natural variantiVAR_0405111120Q → H.1 PublicationCorresponds to variant rs55801437dbSNPEnsembl.1
Natural variantiVAR_0405121137P → L.1 PublicationCorresponds to variant rs56169884dbSNPEnsembl.1
Natural variantiVAR_0405131168S → N.1 PublicationCorresponds to variant rs56326341dbSNPEnsembl.1
Natural variantiVAR_0405141265K → R.1 PublicationCorresponds to variant rs2306242dbSNPEnsembl.1
Natural variantiVAR_0405151297D → N.1 PublicationCorresponds to variant rs1134921dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05447949 – 127Missing in isoform 2. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88435 mRNA. Translation: BAA22623.1.
BC000816 mRNA. Translation: AAH00816.1.
BC008668 mRNA. Translation: AAH08668.1.
BC085005 mRNA. Translation: AAH85005.1.
CCDSiCCDS3340.1. [O14976-1]
CCDS82902.1. [O14976-2]
RefSeqiNP_001305063.1. NM_001318134.1. [O14976-2]
NP_005246.2. NM_005255.3. [O14976-1]
UniGeneiHs.369607.

Genome annotation databases

EnsembliENST00000314167; ENSP00000314499; ENSG00000178950. [O14976-1]
ENST00000511163; ENSP00000421361; ENSG00000178950. [O14976-2]
GeneIDi2580.
KEGGihsa:2580.
UCSCiuc003gbm.6. human. [O14976-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88435 mRNA. Translation: BAA22623.1.
BC000816 mRNA. Translation: AAH00816.1.
BC008668 mRNA. Translation: AAH08668.1.
BC085005 mRNA. Translation: AAH85005.1.
CCDSiCCDS3340.1. [O14976-1]
CCDS82902.1. [O14976-2]
RefSeqiNP_001305063.1. NM_001318134.1. [O14976-2]
NP_005246.2. NM_005255.3. [O14976-1]
UniGeneiHs.369607.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C57X-ray2.55A/B14-351[»]
4C58X-ray2.16A27-351[»]
4C59X-ray2.80A14-351[»]
4O38X-ray2.10A/B12-347[»]
4Y8DX-ray2.10A/B14-351[»]
ProteinModelPortaliO14976.
SMRiO14976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108853. 92 interactors.
IntActiO14976. 80 interactors.
MINTiMINT-5000432.
STRINGi9606.ENSP00000314499.

Chemistry databases

BindingDBiO14976.
ChEMBLiCHEMBL4355.
GuidetoPHARMACOLOGYi2027.

PTM databases

iPTMnetiO14976.
PhosphoSitePlusiO14976.

Polymorphism and mutation databases

BioMutaiGAK.

Proteomic databases

EPDiO14976.
MaxQBiO14976.
PaxDbiO14976.
PeptideAtlasiO14976.
PRIDEiO14976.

Protocols and materials databases

DNASUi2580.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314167; ENSP00000314499; ENSG00000178950. [O14976-1]
ENST00000511163; ENSP00000421361; ENSG00000178950. [O14976-2]
GeneIDi2580.
KEGGihsa:2580.
UCSCiuc003gbm.6. human. [O14976-1]

Organism-specific databases

CTDi2580.
DisGeNETi2580.
GeneCardsiGAK.
H-InvDBHIX0004007.
HGNCiHGNC:4113. GAK.
HPAiHPA027405.
HPA027459.
HPA027463.
MIMi602052. gene.
neXtProtiNX_O14976.
OpenTargetsiENSG00000178950.
PharmGKBiPA28528.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0431. Eukaryota.
KOG1989. Eukaryota.
KOG2283. Eukaryota.
ENOG410Y515. LUCA.
GeneTreeiENSGT00760000119113.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiO14976.
KOiK08855.
OMAiKKNNIRA.
OrthoDBiEOG091G06HJ.
PhylomeDBiO14976.
TreeFamiTF105165.

Enzyme and pathway databases

BioCyciZFISH:HS11332-MONOMER.
ReactomeiR-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-8856828. Clathrin-mediated endocytosis.
SignaLinkiO14976.

Miscellaneous databases

ChiTaRSiGAK. human.
GeneWikiiGAK_(protein).
GenomeRNAii2580.
PROiO14976.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000178950.
CleanExiHS_GAK.
ExpressionAtlasiO14976. baseline and differential.
GenevisibleiO14976. HS.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM01326. PTEN_C2. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAK_HUMAN
AccessioniPrimary (citable) accession number: O14976
Secondary accession number(s): Q5U4P5, Q9BVY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: November 2, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.