Reviewed,
UniProtKB/Swiss-Prot O14976 (GAK_HUMAN)
Last modified
June 16, 2009.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cyclin G-associated kinase EC=2.7.11.1 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1311 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Ref.3 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subcellular location | Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network. Cell junction › focal adhesion Probable. Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions. |
| Tissue specificity | Ubiquitous. Highest in testis. |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 C2 tensin-type domain. Contains 1 J domain. Contains 1 phosphatase tensin-type domain. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell cycle |
| Cellular component | Cell junction Cytoplasm Golgi apparatus |
| Coding sequence diversity | Polymorphism |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesionInferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasmInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW heat shock protein bindingInferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1311 | 1311 | Cyclin G-associated kinase | PRO_0000085958 | |||||
Regions | |||||||||
| Domain | 40 – 314 | 275 | Protein kinase | ||||||
| Domain | 399 – 566 | 168 | Phosphatase tensin-type | ||||||
| Domain | 572 – 710 | 139 | C2 tensin-type | ||||||
| Domain | 1247 – 1311 | 65 | J | ||||||
| Compositional bias | 347 – 350 | 4 | Poly-Pro | ||||||
Sites | |||||||||
| Active site | 173 | 1 | Proton acceptor By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 16 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 456 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 770 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 780 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 826 | 1 | Phosphoserine Ref.4 Ref.5 | ||||||
| Modified residue | 829 | 1 | Phosphoserine Ref.4 Ref.5 | ||||||
| Modified residue | 834 | 1 | Phosphoserine Ref.4 Ref.5 | ||||||
| Modified residue | 1096 | 1 | Phosphoserine Ref.4 | ||||||
Natural variations | |||||||||
| Natural variant | 144 | 1 | S → L Ref.7 | VAR_040505 | |||||
| Natural variant | 580 | 1 | V → M Ref.7 | VAR_040506 | |||||
| Natural variant | 787 | 1 | D → Y Ref.7 | VAR_040507 | |||||
| Natural variant | 877 | 1 | Q → R Ref.7 | VAR_040508 | |||||
| Natural variant | 962 | 1 | G → D in a lung neuroendocrine carcinoma sample; somatic mutation. Ref.7 | VAR_040509 | |||||
| Natural variant | 1051 | 1 | T → M Ref.7 | VAR_040510 | |||||
| Natural variant | 1120 | 1 | Q → H Ref.7 | VAR_040511 | |||||
| Natural variant | 1137 | 1 | P → L Ref.7 | VAR_040512 | |||||
| Natural variant | 1168 | 1 | S → N Ref.7 | VAR_040513 | |||||
| Natural variant | 1265 | 1 | K → R: dbSNP rs2306242. Ref.7 | VAR_040514 | |||||
| Natural variant | 1297 | 1 | D → N: dbSNP rs1134921. Ref.7 | VAR_040515 | |||||
Experimental info | |||||||||
| Sequence conflict | 1113 | 1 | P → A in BAA22623. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure, expression, and chromosomal localization of human GAK." Kimura S.H., Tsuruga H., Yabuta N., Endo Y., Nojima H. Genomics 44:179-187(1997) [PubMed: 9299234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Fibroblast. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 981-1311. Tissue: Placenta. |
| [3] | "Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells." Greener T., Zhao X., Nojima H., Eisenberg E., Greene L.E. J. Biol. Chem. 275:1365-1370(2000) [PubMed: 10625686] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [4] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-456; SER-770; SER-780; SER-826; SER-829; SER-834 AND SER-1096, MASS SPECTROMETRY. |
| [5] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-826; SER-829 AND SER-834, MASS SPECTROMETRY. |
| [6] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [7] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-144; MET-580; TYR-787; ARG-877; ASP-962; MET-1051; HIS-1120; LEU-1137; ASN-1168; ARG-1265 AND ASN-1297. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D88435 mRNA. Translation: BAA22623.1. BC000816 mRNA. Translation: AAH00816.1. BC008668 mRNA. Translation: AAH08668.1. | |
| IPI | IPI00298949. |
| RefSeq | NP_005246.2. |
| UniGene | Hs.369607 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NZ6 based on UniProtKB Q27974. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O14976. 13 interactions. |
PTM databases | |
| PhosphoSite | O14976. |
Proteomic databases | |
| PRIDE | O14976. |
Genome annotation databases | |
| Ensembl | ENSG00000178950. Homo sapiens. [Contig view] |
| GeneID | 2580. |
| KEGG | hsa:2580. |
Organism-specific databases | |
| GeneCards | GC04M000832. |
| HGNC | HGNC:4113. GAK. |
| MIM | 602052. gene. |
| PharmGKB | PA28528. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | O14976. |
| HOVERGEN | O14976. |
| OMA | O14976. FTNIKDT. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 247. |
Gene expression databases | |
| ArrayExpress | O14976. |
| Bgee | O14976. |
| CleanEx | HS_GAK. |
| GermOnline | ENSG00000178950. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR014019. Phosphatase_tensin-typ. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR014020. Tensin_phosphatase_C2-dom. [Graphical view] |
| Pfam | PF00226. DnaJ. 1 hit. PF00069. Pkinase. 1 hit. PF10409. PTEN_C2. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00271. DnaJ. 1 hit. [Graphical view] |
| PROSITE | PS51182. C2_TENSIN. 1 hit. PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. PS51181. PPASE_TENSIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 10201. |
| SOURCE | Search... |
Entry information
| Entry name | GAK_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O14976 Secondary accession number(s): Q9BVY6 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


