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Protein

Very long-chain acyl-CoA synthetase

Gene

SLC27A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precursors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication
ATP + a very-long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 233AMPSequence analysisAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • enzyme binding Source: UniProtKB
  • fatty acid transmembrane transporter activity Source: Ensembl
  • long-chain fatty acid-CoA ligase activity Source: UniProtKB
  • phytanate-CoA ligase activity Source: UniProtKB
  • pristanate-CoA ligase activity Source: UniProtKB
  • signaling receptor binding Source: UniProtKB
  • very long-chain fatty acid-CoA ligase activity Source: UniProtKB

GO - Biological processi

  • bile acid biosynthetic process Source: UniProtKB
  • fatty acid alpha-oxidation Source: UniProtKB
  • fatty acid beta-oxidation Source: UniProtKB
  • long-chain fatty acid import Source: UniProtKB
  • long-chain fatty acid metabolic process Source: UniProtKB
  • methyl-branched fatty acid metabolic process Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • protein targeting to peroxisome Source: Reactome
  • very long-chain fatty acid catabolic process Source: Ensembl

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS06695-MONOMER
ReactomeiR-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-HSA-389599 Alpha-oxidation of phytanate
R-HSA-6798695 Neutrophil degranulation
R-HSA-9033241 Peroxisomal protein import

Protein family/group databases

TCDBi4.C.1.1.5 the fatty acid transporter (fat) family

Chemistry databases

SwissLipidsiSLP:000000428
SLP:000000431 [O14975-2]
SLP:000000432 [O14975-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Very long-chain acyl-CoA synthetase (EC:6.2.1.-)
Short name:
VLACS
Short name:
VLCS
Alternative name(s):
Fatty acid transport protein 2
Short name:
FATP-2
Fatty-acid-coenzyme A ligase, very long-chain 1
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Solute carrier family 27 member 2
THCA-CoA ligase
Very long-chain-fatty-acid-CoA ligase
Gene namesi
Name:SLC27A2
Synonyms:ACSVL1, FACVL1, FATP2, VLACS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000140284.10
HGNCiHGNC:10996 SLC27A2
MIMi603247 gene
neXtProtiNX_O14975

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4Lumenal1 Publication4
Transmembranei5 – 27HelicalSequence analysisAdd BLAST23
Topological domaini28 – 106CytoplasmicSequence analysisAdd BLAST79
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 261LumenalSequence analysisAdd BLAST134
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 620CytoplasmicSequence analysisAdd BLAST338

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi11001
OpenTargetsiENSG00000140284
PharmGKBiPA27971

Chemistry databases

ChEMBLiCHEMBL4326

Polymorphism and mutation databases

BioMutaiSLC27A2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932041 – 620Very long-chain acyl-CoA synthetaseAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei577PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO14975
MaxQBiO14975
PaxDbiO14975
PeptideAtlasiO14975
PRIDEiO14975
ProteomicsDBi48348
48349 [O14975-2]

PTM databases

iPTMnetiO14975
PhosphoSitePlusiO14975

Expressioni

Tissue specificityi

Expressed in liver, kidney, placenta and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000140284
CleanExiHS_SLC27A2
ExpressionAtlasiO14975 baseline and differential
GenevisibleiO14975 HS

Organism-specific databases

HPAiHPA026089

Interactioni

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • signaling receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116194, 52 interactors
IntActiO14975, 10 interactors
MINTiO14975
STRINGi9606.ENSP00000267842

Structurei

3D structure databases

ProteinModelPortaliO14975
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179 Eukaryota
ENOG410XQ8T LUCA
GeneTreeiENSGT00550000074420
HOGENOMiHOG000044189
HOVERGENiHBG005642
InParanoidiO14975
KOiK08746
OMAiVGLHGCI
OrthoDBiEOG091G0B76
PhylomeDBiO14975
TreeFamiTF313430

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR030305 FATP2
PANTHERiPTHR43107:SF13 PTHR43107:SF13, 1 hit
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14975-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSAIYTVLA GLLFLPLLVN LCCPYFFQDI GYFLKVAAVG RRVRSYGKRR
60 70 80 90 100
PARTILRAFL EKARQTPHKP FLLFRDETLT YAQVDRRSNQ VARALHDHLG
110 120 130 140 150
LRQGDCVALL MGNEPAYVWL WLGLVKLGCA MACLNYNIRA KSLLHCFQCC
160 170 180 190 200
GAKVLLVSPE LQAAVEEILP SLKKDDVSIY YVSRTSNTDG IDSFLDKVDE
210 220 230 240 250
VSTEPIPESW RSEVTFSTPA LYIYTSGTTG LPKAAMITHQ RIWYGTGLTF
260 270 280 290 300
VSGLKADDVI YITLPFYHSA ALLIGIHGCI VAGATLALRT KFSASQFWDD
310 320 330 340 350
CRKYNVTVIQ YIGELLRYLC NSPQKPNDRD HKVRLALGNG LRGDVWRQFV
360 370 380 390 400
KRFGDICIYE FYAATEGNIG FMNYARKVGA VGRVNYLQKK IITYDLIKYD
410 420 430 440 450
VEKDEPVRDE NGYCVRVPKG EVGLLVCKIT QLTPFNGYAG AKAQTEKKKL
460 470 480 490 500
RDVFKKGDLY FNSGDLLMVD HENFIYFHDR VGDTFRWKGE NVATTEVADT
510 520 530 540 550
VGLVDFVQEV NVYGVHVPDH EGRIGMASIK MKENHEFDGK KLFQHIADYL
560 570 580 590 600
PSYARPRFLR IQDTIEITGT FKHRKMTLVE EGFNPAVIKD ALYFLDDTAK
610 620
MYVPMTEDIY NAISAKTLKL
Length:620
Mass (Da):70,312
Last modified:October 5, 2010 - v2
Checksum:i752E2FFBB2E47C26
GO
Isoform 2 (identifier: O14975-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     230-282: Missing.

Note: No experimental confirmation available.
Show »
Length:567
Mass (Da):64,616
Checksum:iFF3A2B0FD68177B0
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04653348K → Q4 PublicationsCorresponds to variant dbSNP:rs1648348Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042726230 – 282Missing in isoform 2. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88308 mRNA Translation: BAA23644.1
AF096290 mRNA Translation: AAC64973.1
AK223145 mRNA Translation: BAD96865.1
AK290262 mRNA Translation: BAF82951.1
AC009753 Genomic DNA No translation available.
CH471082 Genomic DNA Translation: EAW77386.1
BC057770 mRNA Translation: AAH57770.1
CCDSiCCDS10133.1 [O14975-1]
CCDS53943.1 [O14975-2]
RefSeqiNP_001153101.1, NM_001159629.1 [O14975-2]
NP_003636.2, NM_003645.3 [O14975-1]
UniGeneiHs.11729

Genome annotation databases

EnsembliENST00000267842; ENSP00000267842; ENSG00000140284 [O14975-1]
ENST00000380902; ENSP00000370289; ENSG00000140284 [O14975-2]
GeneIDi11001
KEGGihsa:11001
UCSCiuc001zxw.4 human [O14975-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Entry informationi

Entry nameiS27A2_HUMAN
AccessioniPrimary (citable) accession number: O14975
Secondary accession number(s): A8K2J7, Q53FY6, Q6PF09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 5, 2010
Last modified: June 20, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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