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O14967

- CLGN_HUMAN

UniProt

O14967 - CLGN_HUMAN

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Protein

Calmegin

Gene

CLGN

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).By similarity

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl
  2. unfolded protein binding Source: ProtInc

GO - Biological processi

  1. binding of sperm to zona pellucida Source: Ensembl
  2. protein complex assembly Source: Ensembl
  3. protein folding Source: InterPro
  4. single fertilization Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Gene namesi
Name:CLGN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:2060. CLGN.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: HPA
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear envelope Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26587.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 610591CalmeginPRO_0000004210Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi151 ↔ 185By similarity
Disulfide bondi351 ↔ 355By similarity
Modified residuei560 – 5601Phosphoserine1 Publication
Modified residuei576 – 5761Phosphoserine1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiO14967.
PaxDbiO14967.
PeptideAtlasiO14967.
PRIDEiO14967.

PTM databases

PhosphoSiteiO14967.

Expressioni

Tissue specificityi

Detected in testis (at protein level). Detected in testis.1 Publication

Gene expression databases

BgeeiO14967.
CleanExiHS_CLGN.
ExpressionAtlasiO14967. baseline and differential.
GenevestigatoriO14967.

Organism-specific databases

HPAiCAB020709.
HPA048761.

Interactioni

Subunit structurei

Interacts with PPIB. Interacts with ADAM2 (By similarity). Interacts with PDILT.By similarity1 Publication

Protein-protein interaction databases

BioGridi107477. 15 interactions.
IntActiO14967. 5 interactions.
STRINGi9606.ENSP00000326699.

Structurei

3D structure databases

ProteinModelPortaliO14967.
SMRiO14967. Positions 51-444.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 471452LumenalSequence AnalysisAdd
BLAST
Topological domaini493 – 610118CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati267 – 280141-1Add
BLAST
Repeati284 – 297141-2Add
BLAST
Repeati303 – 316141-3Add
BLAST
Repeati322 – 335141-4Add
BLAST
Repeati339 – 352142-1Add
BLAST
Repeati356 – 369142-2Add
BLAST
Repeati370 – 383142-3Add
BLAST
Repeati384 – 397142-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni317 – 35034Interaction with PPIBBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG305105.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
HOVERGENiHBG005407.
InParanoidiO14967.
KOiK09551.
OMAiEEKHAKP.
OrthoDBiEOG77126Z.
PhylomeDBiO14967.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O14967-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHFQAFWLCL GLLFISINAE FMDDDVETED FEENSEEIDV NESELSSEIK
60 70 80 90 100
YKTPQPIGEV YFAETFDSGR LAGWVLSKAK KDDMDEEISI YDGRWEIEEL
110 120 130 140 150
KENQVPGDRG LVLKSRAKHH AISAVLAKPF IFADKPLIVQ YEVNFQDGID
160 170 180 190 200
CGGAYIKLLA DTDDLILENF YDKTSYIIMF GPDKCGEDYK LHFIFRHKHP
210 220 230 240 250
KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE VLVDQTVVNK
260 270 280 290 300
GSLLEDVVPP IKPPKEIEDP NDKKPEEWDE RAKIPDPSAV KPEDWDESEP
310 320 330 340 350
AQIEDSSVVK PAGWLDDEPK FIPDPNAEKP DDWNEDTDGE WEAPQILNPA
360 370 380 390 400
CRIGCGEWKP PMIDNPKYKG VWRPPLVDNP NYQGIWSPRK IPNPDYFEDD
410 420 430 440 450
HPFLLTSFSA LGLELWSMTS DIYFDNFIIC SEKEVADHWA ADGWRWKIMI
460 470 480 490 500
ANANKPGVLK QLMAAAEGHP WLWLIYLVTA GVPIALITSF CWPRKVKKKH
510 520 530 540 550
KDTEYKKTDI CIPQTKGVLE QEEKEEKAAL EKPMDLEEEK KQNDGEMLEK
560 570 580 590 600
EEESEPEEKS EEEIEIIEGQ EESNQSNKSG SEDEMKEADE STGSGDGPIK
610
SVRKRRVRKD
Length:610
Mass (Da):70,039
Last modified:January 1, 1998 - v1
Checksum:iF024FC4010D42D7E
GO
Isoform 2 (identifier: O14967-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-211: Missing.
     378-424: Missing.

Note: No experimental confirmation available.

Show »
Length:405
Mass (Da):46,453
Checksum:i6E259D1577FA8BC6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321V → A in BAG63520. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti160 – 1601A → S.
Corresponds to variant rs2567241 [ dbSNP | Ensembl ].
VAR_024400
Natural varianti290 – 2901V → I.
Corresponds to variant rs2175563 [ dbSNP | Ensembl ].
VAR_033776
Natural varianti352 – 3521R → W.
Corresponds to variant rs12513290 [ dbSNP | Ensembl ].
VAR_048590

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 211158Missing in isoform 2. 1 PublicationVSP_055517Add
BLAST
Alternative sequencei378 – 42447Missing in isoform 2. 1 PublicationVSP_055518Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86322 mRNA. Translation: BAA22590.1.
AK093096 mRNA. Translation: BAG52652.1.
AK302149 mRNA. Translation: BAG63520.1.
CH471056 Genomic DNA. Translation: EAX05099.1.
CH471056 Genomic DNA. Translation: EAX05100.1.
CH471056 Genomic DNA. Translation: EAX05101.1.
BC028357 mRNA. Translation: AAH28357.1.
CCDSiCCDS3751.1. [O14967-1]
RefSeqiNP_001124147.1. NM_001130675.1. [O14967-1]
NP_004353.1. NM_004362.2. [O14967-1]
XP_006714125.1. XM_006714062.1. [O14967-1]
UniGeneiHs.86368.

Genome annotation databases

EnsembliENST00000325617; ENSP00000326699; ENSG00000153132. [O14967-1]
ENST00000414773; ENSP00000392782; ENSG00000153132. [O14967-1]
GeneIDi1047.
KEGGihsa:1047.
UCSCiuc003iii.3. human. [O14967-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86322 mRNA. Translation: BAA22590.1 .
AK093096 mRNA. Translation: BAG52652.1 .
AK302149 mRNA. Translation: BAG63520.1 .
CH471056 Genomic DNA. Translation: EAX05099.1 .
CH471056 Genomic DNA. Translation: EAX05100.1 .
CH471056 Genomic DNA. Translation: EAX05101.1 .
BC028357 mRNA. Translation: AAH28357.1 .
CCDSi CCDS3751.1. [O14967-1 ]
RefSeqi NP_001124147.1. NM_001130675.1. [O14967-1 ]
NP_004353.1. NM_004362.2. [O14967-1 ]
XP_006714125.1. XM_006714062.1. [O14967-1 ]
UniGenei Hs.86368.

3D structure databases

ProteinModelPortali O14967.
SMRi O14967. Positions 51-444.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107477. 15 interactions.
IntActi O14967. 5 interactions.
STRINGi 9606.ENSP00000326699.

PTM databases

PhosphoSitei O14967.

Proteomic databases

MaxQBi O14967.
PaxDbi O14967.
PeptideAtlasi O14967.
PRIDEi O14967.

Protocols and materials databases

DNASUi 1047.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000325617 ; ENSP00000326699 ; ENSG00000153132 . [O14967-1 ]
ENST00000414773 ; ENSP00000392782 ; ENSG00000153132 . [O14967-1 ]
GeneIDi 1047.
KEGGi hsa:1047.
UCSCi uc003iii.3. human. [O14967-1 ]

Organism-specific databases

CTDi 1047.
GeneCardsi GC04M141309.
HGNCi HGNC:2060. CLGN.
HPAi CAB020709.
HPA048761.
MIMi 601858. gene.
neXtProti NX_O14967.
PharmGKBi PA26587.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG305105.
GeneTreei ENSGT00430000030841.
HOGENOMi HOG000192436.
HOVERGENi HBG005407.
InParanoidi O14967.
KOi K09551.
OMAi EEKHAKP.
OrthoDBi EOG77126Z.
PhylomeDBi O14967.
TreeFami TF300618.

Miscellaneous databases

ChiTaRSi CLGN. human.
GeneWikii Calmegin.
GenomeRNAii 1047.
NextBioi 35476073.
PROi O14967.
SOURCEi Search...

Gene expression databases

Bgeei O14967.
CleanExi HS_CLGN.
ExpressionAtlasi O14967. baseline and differential.
Genevestigatori O14967.

Family and domain databases

Gene3Di 2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProi IPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view ]
PANTHERi PTHR11073. PTHR11073. 1 hit.
Pfami PF00262. Calreticulin. 1 hit.
[Graphical view ]
PRINTSi PR00626. CALRETICULIN.
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEi PS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone."
    Tanaka H., Ikawa M., Tsuchida J., Nozaki M., Suzuki M., Fujiwara T., Okabe M., Nishimune Y.
    Gene 204:159-163(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-560 AND SER-576, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells."
    van Lith M., Karala A.R., Bown D., Gatehouse J.A., Ruddock L.W., Saunders P.T.K., Benham A.M.
    Mol. Biol. Cell 18:2795-2804(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDILT.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLGN_HUMAN
AccessioniPrimary (citable) accession number: O14967
Secondary accession number(s): B3KS90, B4DXV8, D3DNY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3