O14967 (CLGN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calmegin

Gene names

Name:

CLGN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays an important role in spermatogenesis. Binds calcium ions.
Subunit structure	Interacts with PDILT. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue specificity	Testis.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Chaperone
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	binding of sperm to zona pellucida Inferred from electronic annotation. Source: Compara protein complex assembly Inferred from electronic annotation. Source: Compara protein folding Inferred from electronic annotation. Source: InterPro single fertilization Traceable author statement PubMed 9177349. Source: ProtInc
Cellular_component	endoplasmic reticulum Traceable author statement PubMed 9177349. Source: ProtInc endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Traceable author statement PubMed 9177349. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 610	591	Calmegin		PRO_0000004210
Regions
	Topological domain	20 – 471	452	Lumenal Potential
	Transmembrane	472 – 492	21	Helical; Potential
	Topological domain	493 – 610	118	Cytoplasmic Potential
	Repeat	267 – 280	14	1-1
	Repeat	284 – 297	14	1-2
	Repeat	303 – 316	14	1-3
	Repeat	322 – 335	14	1-4
	Repeat	339 – 352	14	2-1
	Repeat	356 – 369	14	2-2
	Repeat	370 – 383	14	2-3
	Repeat	384 – 397	14	2-4
Amino acid modifications
	Modified residue	560	1	Phosphoserine Ref.5
	Modified residue	576	1	Phosphoserine Ref.5
	Disulfide bond	151 ↔ 185		By similarity
	Disulfide bond	351 ↔ 355		By similarity
Natural variations
	Natural variant	160	1	A → S. Corresponds to variant rs2567241 [ dbSNP | Ensembl ].		VAR_024400
	Natural variant	290	1	V → I. Corresponds to variant rs2175563 [ dbSNP | Ensembl ].		VAR_033776
	Natural variant	352	1	R → W. Corresponds to variant rs12513290 [ dbSNP | Ensembl ].		VAR_048590

Sequences

	Sequence		Length	Mass (Da)
	O14967 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F024FC4010D42D7E	FASTA	610	70,039
	10 20 30 40 50 60 MHFQAFWLCL GLLFISINAE FMDDDVETED FEENSEEIDV NESELSSEIK YKTPQPIGEV 70 80 90 100 110 120 YFAETFDSGR LAGWVLSKAK KDDMDEEISI YDGRWEIEEL KENQVPGDRG LVLKSRAKHH 130 140 150 160 170 180 AISAVLAKPF IFADKPLIVQ YEVNFQDGID CGGAYIKLLA DTDDLILENF YDKTSYIIMF 190 200 210 220 230 240 GPDKCGEDYK LHFIFRHKHP KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE 250 260 270 280 290 300 VLVDQTVVNK GSLLEDVVPP IKPPKEIEDP NDKKPEEWDE RAKIPDPSAV KPEDWDESEP 310 320 330 340 350 360 AQIEDSSVVK PAGWLDDEPK FIPDPNAEKP DDWNEDTDGE WEAPQILNPA CRIGCGEWKP 370 380 390 400 410 420 PMIDNPKYKG VWRPPLVDNP NYQGIWSPRK IPNPDYFEDD HPFLLTSFSA LGLELWSMTS 430 440 450 460 470 480 DIYFDNFIIC SEKEVADHWA ADGWRWKIMI ANANKPGVLK QLMAAAEGHP WLWLIYLVTA 490 500 510 520 530 540 GVPIALITSF CWPRKVKKKH KDTEYKKTDI CIPQTKGVLE QEEKEEKAAL EKPMDLEEEK 550 560 570 580 590 600 KQNDGEMLEK EEESEPEEKS EEEIEIIEGQ EESNQSNKSG SEDEMKEADE STGSGDGPIK 610 SVRKRRVRKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone." Tanaka H., Ikawa M., Tsuchida J., Nozaki M., Suzuki M., Fujiwara T., Okabe M., Nishimune Y. Gene 204:159-163(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-560 AND SER-576, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells." van Lith M., Karala A.R., Bown D., Gatehouse J.A., Ruddock L.W., Saunders P.T.K., Benham A.M. Mol. Biol. Cell 18:2795-2804(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PDILT.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86322 mRNA. Translation: BAA22590.1. AK093096 mRNA. Translation: BAG52652.1. CH471056 Genomic DNA. Translation: EAX05099.1. CH471056 Genomic DNA. Translation: EAX05100.1. CH471056 Genomic DNA. Translation: EAX05101.1. BC028357 mRNA. Translation: AAH28357.1.
IPI	IPI00024776.
RefSeq	NP_001124147.1. NM_001130675.1. NP_004353.1. NM_004362.2.
UniGene	Hs.86368.
3D structure databases
ProteinModelPortal	O14967.
SMR	O14967. Positions 51-444.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14967. 5 interactions.
STRING	9606.ENSP00000326699.
PTM databases
PhosphoSite	O14967.
Proteomic databases
PaxDb	O14967.
PeptideAtlas	O14967.
PRIDE	O14967.
Protocols and materials databases
DNASU	1047.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000325617; ENSP00000326699; ENSG00000153132. ENST00000414773; ENSP00000392782; ENSG00000153132. ENST00000537281; ENSP00000439381; ENSG00000153132.
GeneID	1047.
KEGG	hsa:1047.
UCSC	uc003iii.3. human.
Organism-specific databases
CTD	1047.
GeneCards	GC04M141309.
HGNC	HGNC:2060. CLGN.
HPA	CAB020709.
MIM	601858. gene.
neXtProt	NX_O14967.
PharmGKB	PA26587.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG305105.
HOGENOM	HOG000192436.
HOVERGEN	HBG005407.
InParanoid	O14967.
KO	K09551.
OMA	EEKHAKP.
OrthoDB	EOG48D0V1.
PhylomeDB	O14967.
Gene expression databases
ArrayExpress	O14967.
Bgee	O14967.
CleanEx	HS_CLGN.
Genevestigator	O14967.
GermOnline	ENSG00000153132. Homo sapiens.
Family and domain databases
Gene3D	2.10.250.10. 1 hit. 2.60.120.200. 2 hits.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 2 hits.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CLGN. human.
GenomeRNAi	1047.
NextBio	4389.
SOURCE	Search...

Entry information

Entry name

CLGN_HUMAN

Accession

Primary (citable) accession number: O14967
Secondary accession number(s): B3KS90, D3DNY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.