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O14967 (CLGN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calmegin
Gene names
Name:CLGN
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions By similarity.

Subunit structure

Interacts with PPIB. Interacts with ADAM2 By similarity. Interacts with PDILT. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Tissue specificity

Detected in testis (at protein level). Detected in testis. Ref.1

Sequence similarities

Belongs to the calreticulin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 610591Calmegin
PRO_0000004210

Regions

Topological domain20 – 471452Lumenal Potential
Transmembrane472 – 49221Helical; Potential
Topological domain493 – 610118Cytoplasmic Potential
Repeat267 – 280141-1
Repeat284 – 297141-2
Repeat303 – 316141-3
Repeat322 – 335141-4
Repeat339 – 352142-1
Repeat356 – 369142-2
Repeat370 – 383142-3
Repeat384 – 397142-4
Region317 – 35034Interaction with PPIB By similarity

Amino acid modifications

Modified residue5601Phosphoserine Ref.5
Modified residue5761Phosphoserine Ref.5
Disulfide bond151 ↔ 185 By similarity
Disulfide bond351 ↔ 355 By similarity

Natural variations

Natural variant1601A → S.
Corresponds to variant rs2567241 [ dbSNP | Ensembl ].
VAR_024400
Natural variant2901V → I.
Corresponds to variant rs2175563 [ dbSNP | Ensembl ].
VAR_033776
Natural variant3521R → W.
Corresponds to variant rs12513290 [ dbSNP | Ensembl ].
VAR_048590

Sequences

Sequence LengthMass (Da)Tools
O14967 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: F024FC4010D42D7E

FASTA61070,039
        10         20         30         40         50         60 
MHFQAFWLCL GLLFISINAE FMDDDVETED FEENSEEIDV NESELSSEIK YKTPQPIGEV 

        70         80         90        100        110        120 
YFAETFDSGR LAGWVLSKAK KDDMDEEISI YDGRWEIEEL KENQVPGDRG LVLKSRAKHH 

       130        140        150        160        170        180 
AISAVLAKPF IFADKPLIVQ YEVNFQDGID CGGAYIKLLA DTDDLILENF YDKTSYIIMF 

       190        200        210        220        230        240 
GPDKCGEDYK LHFIFRHKHP KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE 

       250        260        270        280        290        300 
VLVDQTVVNK GSLLEDVVPP IKPPKEIEDP NDKKPEEWDE RAKIPDPSAV KPEDWDESEP 

       310        320        330        340        350        360 
AQIEDSSVVK PAGWLDDEPK FIPDPNAEKP DDWNEDTDGE WEAPQILNPA CRIGCGEWKP 

       370        380        390        400        410        420 
PMIDNPKYKG VWRPPLVDNP NYQGIWSPRK IPNPDYFEDD HPFLLTSFSA LGLELWSMTS 

       430        440        450        460        470        480 
DIYFDNFIIC SEKEVADHWA ADGWRWKIMI ANANKPGVLK QLMAAAEGHP WLWLIYLVTA 

       490        500        510        520        530        540 
GVPIALITSF CWPRKVKKKH KDTEYKKTDI CIPQTKGVLE QEEKEEKAAL EKPMDLEEEK 

       550        560        570        580        590        600 
KQNDGEMLEK EEESEPEEKS EEEIEIIEGQ EESNQSNKSG SEDEMKEADE STGSGDGPIK 

       610 
SVRKRRVRKD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone."
Tanaka H., Ikawa M., Tsuchida J., Nozaki M., Suzuki M., Fujiwara T., Okabe M., Nishimune Y.
Gene 204:159-163(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-560 AND SER-576, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells."
van Lith M., Karala A.R., Bown D., Gatehouse J.A., Ruddock L.W., Saunders P.T.K., Benham A.M.
Mol. Biol. Cell 18:2795-2804(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PDILT.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86322 mRNA. Translation: BAA22590.1.
AK093096 mRNA. Translation: BAG52652.1.
CH471056 Genomic DNA. Translation: EAX05099.1.
CH471056 Genomic DNA. Translation: EAX05100.1.
CH471056 Genomic DNA. Translation: EAX05101.1.
BC028357 mRNA. Translation: AAH28357.1.
CCDSCCDS3751.1.
RefSeqNP_001124147.1. NM_001130675.1.
NP_004353.1. NM_004362.2.
XP_006714125.1. XM_006714062.1.
UniGeneHs.86368.

3D structure databases

ProteinModelPortalO14967.
SMRO14967. Positions 51-444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107477. 11 interactions.
IntActO14967. 5 interactions.
STRING9606.ENSP00000326699.

PTM databases

PhosphoSiteO14967.

Proteomic databases

MaxQBO14967.
PaxDbO14967.
PeptideAtlasO14967.
PRIDEO14967.

Protocols and materials databases

DNASU1047.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000325617; ENSP00000326699; ENSG00000153132.
ENST00000414773; ENSP00000392782; ENSG00000153132.
ENST00000537281; ENSP00000439381; ENSG00000153132.
GeneID1047.
KEGGhsa:1047.
UCSCuc003iii.3. human.

Organism-specific databases

CTD1047.
GeneCardsGC04M141309.
HGNCHGNC:2060. CLGN.
HPACAB020709.
HPA048761.
MIM601858. gene.
neXtProtNX_O14967.
PharmGKBPA26587.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG305105.
HOGENOMHOG000192436.
HOVERGENHBG005407.
InParanoidO14967.
KOK09551.
OMAEEKHAKP.
OrthoDBEOG77126Z.
PhylomeDBO14967.
TreeFamTF300618.

Gene expression databases

ArrayExpressO14967.
BgeeO14967.
CleanExHS_CLGN.
GenevestigatorO14967.

Family and domain databases

Gene3D2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERPTHR11073. PTHR11073. 1 hit.
PfamPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSPR00626. CALRETICULIN.
SUPFAMSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCLGN. human.
GeneWikiCalmegin.
GenomeRNAi1047.
NextBio4389.
PROO14967.
SOURCESearch...

Entry information

Entry nameCLGN_HUMAN
AccessionPrimary (citable) accession number: O14967
Secondary accession number(s): B3KS90, D3DNY8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM