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O14967

- CLGN_HUMAN

UniProt

O14967 - CLGN_HUMAN

Protein

Calmegin

Gene

CLGN

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions By similarity.By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. unfolded protein binding Source: ProtInc

    GO - Biological processi

    1. binding of sperm to zona pellucida Source: Ensembl
    2. protein complex assembly Source: Ensembl
    3. protein folding Source: InterPro
    4. single fertilization Source: ProtInc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calmegin
    Gene namesi
    Name:CLGN
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:2060. CLGN.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: HPA
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear envelope Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26587.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 610591CalmeginPRO_0000004210Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi151 ↔ 185By similarity
    Disulfide bondi351 ↔ 355By similarity
    Modified residuei560 – 5601Phosphoserine1 Publication
    Modified residuei576 – 5761Phosphoserine1 Publication

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiO14967.
    PaxDbiO14967.
    PeptideAtlasiO14967.
    PRIDEiO14967.

    PTM databases

    PhosphoSiteiO14967.

    Expressioni

    Tissue specificityi

    Detected in testis (at protein level). Detected in testis.1 Publication

    Gene expression databases

    ArrayExpressiO14967.
    BgeeiO14967.
    CleanExiHS_CLGN.
    GenevestigatoriO14967.

    Organism-specific databases

    HPAiCAB020709.
    HPA048761.

    Interactioni

    Subunit structurei

    Interacts with PPIB. Interacts with ADAM2 By similarity. Interacts with PDILT.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi107477. 11 interactions.
    IntActiO14967. 5 interactions.
    STRINGi9606.ENSP00000326699.

    Structurei

    3D structure databases

    ProteinModelPortaliO14967.
    SMRiO14967. Positions 51-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 471452LumenalSequence AnalysisAdd
    BLAST
    Topological domaini493 – 610118CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei472 – 49221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati267 – 280141-1Add
    BLAST
    Repeati284 – 297141-2Add
    BLAST
    Repeati303 – 316141-3Add
    BLAST
    Repeati322 – 335141-4Add
    BLAST
    Repeati339 – 352142-1Add
    BLAST
    Repeati356 – 369142-2Add
    BLAST
    Repeati370 – 383142-3Add
    BLAST
    Repeati384 – 397142-4Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni317 – 35034Interaction with PPIBBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305105.
    HOGENOMiHOG000192436.
    HOVERGENiHBG005407.
    InParanoidiO14967.
    KOiK09551.
    OMAiEEKHAKP.
    OrthoDBiEOG77126Z.
    PhylomeDBiO14967.
    TreeFamiTF300618.

    Family and domain databases

    Gene3Di2.10.250.10. 1 hit.
    2.60.120.200. 2 hits.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O14967-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHFQAFWLCL GLLFISINAE FMDDDVETED FEENSEEIDV NESELSSEIK    50
    YKTPQPIGEV YFAETFDSGR LAGWVLSKAK KDDMDEEISI YDGRWEIEEL 100
    KENQVPGDRG LVLKSRAKHH AISAVLAKPF IFADKPLIVQ YEVNFQDGID 150
    CGGAYIKLLA DTDDLILENF YDKTSYIIMF GPDKCGEDYK LHFIFRHKHP 200
    KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE VLVDQTVVNK 250
    GSLLEDVVPP IKPPKEIEDP NDKKPEEWDE RAKIPDPSAV KPEDWDESEP 300
    AQIEDSSVVK PAGWLDDEPK FIPDPNAEKP DDWNEDTDGE WEAPQILNPA 350
    CRIGCGEWKP PMIDNPKYKG VWRPPLVDNP NYQGIWSPRK IPNPDYFEDD 400
    HPFLLTSFSA LGLELWSMTS DIYFDNFIIC SEKEVADHWA ADGWRWKIMI 450
    ANANKPGVLK QLMAAAEGHP WLWLIYLVTA GVPIALITSF CWPRKVKKKH 500
    KDTEYKKTDI CIPQTKGVLE QEEKEEKAAL EKPMDLEEEK KQNDGEMLEK 550
    EEESEPEEKS EEEIEIIEGQ EESNQSNKSG SEDEMKEADE STGSGDGPIK 600
    SVRKRRVRKD 610
    Length:610
    Mass (Da):70,039
    Last modified:January 1, 1998 - v1
    Checksum:iF024FC4010D42D7E
    GO
    Isoform 2 (identifier: O14967-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         54-211: Missing.
         378-424: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:405
    Mass (Da):46,453
    Checksum:i6E259D1577FA8BC6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti232 – 2321V → A in BAG63520. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti160 – 1601A → S.
    Corresponds to variant rs2567241 [ dbSNP | Ensembl ].
    VAR_024400
    Natural varianti290 – 2901V → I.
    Corresponds to variant rs2175563 [ dbSNP | Ensembl ].
    VAR_033776
    Natural varianti352 – 3521R → W.
    Corresponds to variant rs12513290 [ dbSNP | Ensembl ].
    VAR_048590

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei54 – 211158Missing in isoform 2. 1 PublicationVSP_055517Add
    BLAST
    Alternative sequencei378 – 42447Missing in isoform 2. 1 PublicationVSP_055518Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86322 mRNA. Translation: BAA22590.1.
    AK093096 mRNA. Translation: BAG52652.1.
    AK302149 mRNA. Translation: BAG63520.1.
    CH471056 Genomic DNA. Translation: EAX05099.1.
    CH471056 Genomic DNA. Translation: EAX05100.1.
    CH471056 Genomic DNA. Translation: EAX05101.1.
    BC028357 mRNA. Translation: AAH28357.1.
    CCDSiCCDS3751.1.
    RefSeqiNP_001124147.1. NM_001130675.1.
    NP_004353.1. NM_004362.2.
    XP_006714125.1. XM_006714062.1.
    UniGeneiHs.86368.

    Genome annotation databases

    EnsembliENST00000325617; ENSP00000326699; ENSG00000153132. [O14967-1]
    ENST00000414773; ENSP00000392782; ENSG00000153132. [O14967-1]
    GeneIDi1047.
    KEGGihsa:1047.
    UCSCiuc003iii.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86322 mRNA. Translation: BAA22590.1 .
    AK093096 mRNA. Translation: BAG52652.1 .
    AK302149 mRNA. Translation: BAG63520.1 .
    CH471056 Genomic DNA. Translation: EAX05099.1 .
    CH471056 Genomic DNA. Translation: EAX05100.1 .
    CH471056 Genomic DNA. Translation: EAX05101.1 .
    BC028357 mRNA. Translation: AAH28357.1 .
    CCDSi CCDS3751.1.
    RefSeqi NP_001124147.1. NM_001130675.1.
    NP_004353.1. NM_004362.2.
    XP_006714125.1. XM_006714062.1.
    UniGenei Hs.86368.

    3D structure databases

    ProteinModelPortali O14967.
    SMRi O14967. Positions 51-444.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107477. 11 interactions.
    IntActi O14967. 5 interactions.
    STRINGi 9606.ENSP00000326699.

    PTM databases

    PhosphoSitei O14967.

    Proteomic databases

    MaxQBi O14967.
    PaxDbi O14967.
    PeptideAtlasi O14967.
    PRIDEi O14967.

    Protocols and materials databases

    DNASUi 1047.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000325617 ; ENSP00000326699 ; ENSG00000153132 . [O14967-1 ]
    ENST00000414773 ; ENSP00000392782 ; ENSG00000153132 . [O14967-1 ]
    GeneIDi 1047.
    KEGGi hsa:1047.
    UCSCi uc003iii.3. human.

    Organism-specific databases

    CTDi 1047.
    GeneCardsi GC04M141309.
    HGNCi HGNC:2060. CLGN.
    HPAi CAB020709.
    HPA048761.
    MIMi 601858. gene.
    neXtProti NX_O14967.
    PharmGKBi PA26587.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG305105.
    HOGENOMi HOG000192436.
    HOVERGENi HBG005407.
    InParanoidi O14967.
    KOi K09551.
    OMAi EEKHAKP.
    OrthoDBi EOG77126Z.
    PhylomeDBi O14967.
    TreeFami TF300618.

    Miscellaneous databases

    ChiTaRSi CLGN. human.
    GeneWikii Calmegin.
    GenomeRNAii 1047.
    NextBioi 4389.
    PROi O14967.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14967.
    Bgeei O14967.
    CleanExi HS_CLGN.
    Genevestigatori O14967.

    Family and domain databases

    Gene3Di 2.10.250.10. 1 hit.
    2.60.120.200. 2 hits.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone."
      Tanaka H., Ikawa M., Tsuchida J., Nozaki M., Suzuki M., Fujiwara T., Okabe M., Nishimune Y.
      Gene 204:159-163(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-560 AND SER-576, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells."
      van Lith M., Karala A.R., Bown D., Gatehouse J.A., Ruddock L.W., Saunders P.T.K., Benham A.M.
      Mol. Biol. Cell 18:2795-2804(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDILT.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCLGN_HUMAN
    AccessioniPrimary (citable) accession number: O14967
    Secondary accession number(s): B3KS90, B4DXV8, D3DNY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3