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Protein

Phospholipase D2

Gene

PLD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.By similarity

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulationi

Stimulated by phosphatidylinositol 4,5-bisphosphate and activated by the ADP-ribosylation factor-1 (ARF-1).

GO - Molecular functioni

  1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  2. phosphatidylinositol binding Source: InterPro
  3. phospholipase D activity Source: ProtInc

GO - Biological processi

  1. cytoskeleton organization Source: ProtInc
  2. Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  3. glycerophospholipid biosynthetic process Source: Reactome
  4. G-protein coupled receptor internalization Source: Ensembl
  5. innate immune response Source: Reactome
  6. lipid catabolic process Source: UniProtKB-KW
  7. phosphatidic acid biosynthetic process Source: Reactome
  8. phosphatidylglycerol biosynthetic process Source: Reactome
  9. phospholipid metabolic process Source: Reactome
  10. small GTPase mediated signal transduction Source: ProtInc
  11. small molecule metabolic process Source: Reactome
  12. synaptic vesicle recycling Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.4. 2681.
4.2.1.1. 2681.
ReactomeiREACT_120906. Synthesis of PA.
REACT_121280. Synthesis of PG.
REACT_160158. Role of phospholipids in phagocytosis.
SignaLinkiO14939.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D2 (EC:3.1.4.4)
Short name:
PLD 2
Short name:
hPLD2
Alternative name(s):
Choline phosphatase 2
PLD1C
Phosphatidylcholine-hydrolyzing phospholipase D2
Gene namesi
Name:PLD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:9068. PLD2.

Subcellular locationi

  1. Membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. brush border membrane Source: Ensembl
  2. endoplasmic reticulum membrane Source: Reactome
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33397.

Chemistry

DrugBankiDB00122. Choline.

Polymorphism and mutation databases

BioMutaiPLD2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Phospholipase D2PRO_0000218805Add
BLAST

Post-translational modificationi

Phosphorylated by FGR.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14939.
PaxDbiO14939.
PRIDEiO14939.

PTM databases

PhosphoSiteiO14939.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiO14939.
CleanExiHS_PLD2.
ExpressionAtlasiO14939. baseline and differential.
GenevestigatoriO14939.

Organism-specific databases

HPAiHPA013397.

Interactioni

Subunit structurei

Interacts with EGFR (By similarity). Interacts with PIP5K1B.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CFL1P235282EBI-1053996,EBI-352733
GRB2P629934EBI-1053996,EBI-401755

Protein-protein interaction databases

BioGridi111354. 27 interactions.
DIPiDIP-38903N.
IntActiO14939. 3 interactions.
MINTiMINT-141629.
STRINGi9606.ENSP00000263088.

Structurei

3D structure databases

ProteinModelPortaliO14939.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 195131PXPROSITE-ProRule annotationAdd
BLAST
Domaini203 – 311109PHAdd
BLAST
Domaini437 – 46428PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini751 – 77828PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni441 – 788348CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the phospholipase D family.Curated
Contains 1 PH domain.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1502.
GeneTreeiENSGT00390000008356.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
InParanoidiO14939.
KOiK01115.
OMAiEPSLMNG.
OrthoDBiEOG7N63KT.
PhylomeDBiO14939.
TreeFamiTF300589.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D1/D2.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform PLD2A (identifier: O14939-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ
60 70 80 90 100
SLKVHPLVFA PGVPVTAQVV GTERYTSGSK VGTCTLYSVR LTHGDFSWTT
110 120 130 140 150
KKKYRHFQEL HRDLLRHKVL MSLLPLARFA VAYSPARDAG NREMPSLPRA
160 170 180 190 200
GPEGSTRHAA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL
210 220 230 240 250
GRKGLEGMIR KRSGGHRVPG LTCCGRDQVC YRWSKRWLVV KDSFLLYMCL
260 270 280 290 300
ETGAISFVQL FDPGFEVQVG KRSTEARHGV RIDTSHRSLI LKCSSYRQAR
310 320 330 340 350
WWAQEITELA QGPGRDFLQL HRHDSYAPPR PGTLARWFVN GAGYFAAVAD
360 370 380 390 400
AILRAQEEIF ITDWWLSPEV YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL
410 420 430 440 450
LFKEVELALG INSGYSKRAL MLLHPNIKVM RHPDQVTLWA HHEKLLVVDQ
460 470 480 490 500
VVAFLGGLDL AYGRWDDLHY RLTDLGDSSE SAASQPPTPR PDSPATPDLS
510 520 530 540 550
HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGL
560 570 580 590 600
PARDLARHFI QRWNFTKTTK AKYKTPTYPY LLPKSTSTAN QLPFTLPGGQ
610 620 630 640 650
CTTVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG
660 670 680 690 700
RTVLNKVGDE IVDRILKAHK QGWCYRVYVL LPLLPGFEGD ISTGGGNSIQ
710 720 730 740 750
AILHFTYRTL CRGEYSILHR LKAAMGTAWR DYISICGLRT HGELGGHPVS
760 770 780 790 800
ELIYIHSKVL IADDRTVIIG SANINDRSLL GKRDSELAVL IEDTETEPSL
810 820 830 840 850
MNGAEYQAGR FALSLRKHCF GVILGANTRP DLDLRDPICD DFFQLWQDMA
860 870 880 890 900
ESNANIYEQI FRCLPSNATR SLRTLREYVA VEPLATVSPP LARSELTQVQ
910 920 930
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT
Length:933
Mass (Da):105,987
Last modified:February 21, 2001 - v2
Checksum:iF25F6B73B45F57ED
GO
Isoform PLD2C (identifier: O14939-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     337-933: Missing.

Show »
Length:336
Mass (Da):38,252
Checksum:iB88330C3765169E3
GO
Isoform PLD2B (identifier: O14939-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     810-821: RFALSLRKHCFG → S

Show »
Length:922
Mass (Da):104,657
Checksum:iC160953BA88F8F19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161D → G in AAB96656 (Ref. 3) Curated
Sequence conflicti165 – 1651Y → C in AAB96656 (Ref. 3) Curated
Sequence conflicti212 – 2121R → C in AAB96656 (Ref. 3) Curated
Sequence conflicti268 – 2681Q → R in AAB96655 (Ref. 3) Curated
Sequence conflicti325 – 3251S → R in AAB96656 (Ref. 3) Curated
Sequence conflicti445 – 4451L → P in AAB96656 (Ref. 3) Curated
Sequence conflicti510 – 5101D → G in AAB96656 (Ref. 3) Curated
Sequence conflicti566 – 5661T → I in AAB96655 (Ref. 3) Curated
Sequence conflicti784 – 7841D → G in AAB96656 (Ref. 3) Curated
Sequence conflicti801 – 8022MN → ID in AAC24498 (PubMed:9582313).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti172 – 1721R → C.2 Publications
Corresponds to variant rs2286672 [ dbSNP | Ensembl ].
VAR_051704
Natural varianti577 – 5771T → I.2 Publications
Corresponds to variant rs1052748 [ dbSNP | Ensembl ].
VAR_051705
Natural varianti632 – 6321E → G.2 Publications
Corresponds to variant rs17854914 [ dbSNP | Ensembl ].
VAR_061750
Natural varianti804 – 8041A → T.
Corresponds to variant rs11545163 [ dbSNP | Ensembl ].
VAR_051706
Natural varianti807 – 8071Q → E in a breast cancer sample; somatic mutation. 1 Publication
VAR_036503
Natural varianti821 – 8211G → R.
Corresponds to variant rs3764897 [ dbSNP | Ensembl ].
VAR_051707
Natural varianti821 – 8211G → S.1 Publication
Corresponds to variant rs3764897 [ dbSNP | Ensembl ].
VAR_059774

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei337 – 933597Missing in isoform PLD2C. 1 PublicationVSP_005027Add
BLAST
Alternative sequencei810 – 82112RFALS…KHCFG → S in isoform PLD2B. 2 PublicationsVSP_054769Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033850 mRNA. Translation: AAD04197.1.
AF035483 mRNA. Translation: AAC24498.1.
AF038440 mRNA. Translation: AAB96655.1.
AF038441 mRNA. Translation: AAB96656.1.
AC233723 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90405.1.
BC015033 mRNA. Translation: AAH15033.1.
BC056871 mRNA. Translation: AAH56871.1.
CCDSiCCDS11057.1. [O14939-1]
CCDS58507.1. [O14939-4]
RefSeqiNP_001230037.1. NM_001243108.1. [O14939-4]
NP_002654.3. NM_002663.4. [O14939-1]
UniGeneiHs.104519.

Genome annotation databases

EnsembliENST00000263088; ENSP00000263088; ENSG00000129219. [O14939-1]
ENST00000572940; ENSP00000459571; ENSG00000129219. [O14939-4]
GeneIDi5338.
KEGGihsa:5338.
UCSCiuc002fzc.3. human. [O14939-1]
uc002fzd.3. human.

Polymorphism and mutation databases

BioMutaiPLD2.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Phospholipase D entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033850 mRNA. Translation: AAD04197.1.
AF035483 mRNA. Translation: AAC24498.1.
AF038440 mRNA. Translation: AAB96655.1.
AF038441 mRNA. Translation: AAB96656.1.
AC233723 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90405.1.
BC015033 mRNA. Translation: AAH15033.1.
BC056871 mRNA. Translation: AAH56871.1.
CCDSiCCDS11057.1. [O14939-1]
CCDS58507.1. [O14939-4]
RefSeqiNP_001230037.1. NM_001243108.1. [O14939-4]
NP_002654.3. NM_002663.4. [O14939-1]
UniGeneiHs.104519.

3D structure databases

ProteinModelPortaliO14939.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111354. 27 interactions.
DIPiDIP-38903N.
IntActiO14939. 3 interactions.
MINTiMINT-141629.
STRINGi9606.ENSP00000263088.

Chemistry

BindingDBiO14939.
ChEMBLiCHEMBL2734.
DrugBankiDB00122. Choline.
GuidetoPHARMACOLOGYi1434.

PTM databases

PhosphoSiteiO14939.

Polymorphism and mutation databases

BioMutaiPLD2.

Proteomic databases

MaxQBiO14939.
PaxDbiO14939.
PRIDEiO14939.

Protocols and materials databases

DNASUi5338.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263088; ENSP00000263088; ENSG00000129219. [O14939-1]
ENST00000572940; ENSP00000459571; ENSG00000129219. [O14939-4]
GeneIDi5338.
KEGGihsa:5338.
UCSCiuc002fzc.3. human. [O14939-1]
uc002fzd.3. human.

Organism-specific databases

CTDi5338.
GeneCardsiGC17P004710.
H-InvDBHIX0013451.
HGNCiHGNC:9068. PLD2.
HPAiHPA013397.
MIMi602384. gene.
neXtProtiNX_O14939.
PharmGKBiPA33397.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1502.
GeneTreeiENSGT00390000008356.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
InParanoidiO14939.
KOiK01115.
OMAiEPSLMNG.
OrthoDBiEOG7N63KT.
PhylomeDBiO14939.
TreeFamiTF300589.

Enzyme and pathway databases

BRENDAi3.1.4.4. 2681.
4.2.1.1. 2681.
ReactomeiREACT_120906. Synthesis of PA.
REACT_121280. Synthesis of PG.
REACT_160158. Role of phospholipids in phagocytosis.
SignaLinkiO14939.

Miscellaneous databases

ChiTaRSiPLD2. human.
GeneWikiiPLD2.
GenomeRNAii5338.
NextBioi20676.
PROiO14939.
SOURCEiSearch...

Gene expression databases

BgeeiO14939.
CleanExiHS_PLD2.
ExpressionAtlasiO14939. baseline and differential.
GenevestigatoriO14939.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D1/D2.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of human PLD2 and the analysis of PLD isoform splice variants."
    Steed P.M., Clark K.L., Boyar W.C., Lasala D.J.
    FASEB J. 12:1309-1317(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PLD2A; PLD2B AND PLD2C), CHARACTERIZATION, VARIANT CYS-172.
    Tissue: Brain.
  2. "Cloning and initial characterization of a human phospholipase D2 (hPLD2). ADP-ribosylation factor regulates hPLD2."
    Lopez I., Arnold R.S., Lambeth J.D.
    J. Biol. Chem. 273:12846-12852(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PLD2A), CHARACTERIZATION, VARIANT ILE-577.
    Tissue: B-cell.
  3. Saqib K.M., Clark J.M., Byrd P.J., Armstrong S.J., Wakelam M.J.O.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PLD2A AND PLD2B), VARIANTS GLY-632 AND SER-821.
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PLD2A), VARIANTS CYS-172; ILE-577 AND GLY-632.
    Tissue: Skin and Uterus.
  7. "Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity."
    Divecha N., Roefs M., Halstead J.R., D'Andrea S., Fernandez-Borga M., Oomen L., Saqib K.M., Wakelam M.J.O., D'Santos C.
    EMBO J. 19:5440-5449(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIP5K1B.
  8. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-807.

Entry informationi

Entry nameiPLD2_HUMAN
AccessioniPrimary (citable) accession number: O14939
Secondary accession number(s): I3L2C9
, O43540, O43579, O43580, Q6PGR0, Q96BY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: April 29, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.