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Protein

Phospholipase D2

Gene

PLD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.By similarity

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulationi

Stimulated by phosphatidylinositol 4,5-bisphosphate and activated by the ADP-ribosylation factor-1 (ARF-1).

GO - Molecular functioni

GO - Biological processi

  • cell motility Source: GO_Central
  • cytoskeleton organization Source: ProtInc
  • Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  • inositol lipid-mediated signaling Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • phosphatidic acid biosynthetic process Source: Reactome
  • small GTPase mediated signal transduction Source: ProtInc
  • synaptic vesicle recycling Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciZFISH:HS05256-MONOMER.
BRENDAi3.1.4.4. 2681.
4.2.1.1. 2681.
ReactomeiR-HSA-1483148. Synthesis of PG.
R-HSA-1483166. Synthesis of PA.
R-HSA-2029485. Role of phospholipids in phagocytosis.
SignaLinkiO14939.
SIGNORiO14939.

Chemistry databases

SwissLipidsiSLP:000000108.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D2 (EC:3.1.4.4)
Short name:
PLD 2
Short name:
hPLD2
Alternative name(s):
Choline phosphatase 2
PLD1C
Phosphatidylcholine-hydrolyzing phospholipase D2
Gene namesi
Name:PLD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:9068. PLD2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5338.
OpenTargetsiENSG00000129219.
PharmGKBiPA33397.

Chemistry databases

ChEMBLiCHEMBL2734.
DrugBankiDB00122. Choline.
GuidetoPHARMACOLOGYi1434.

Polymorphism and mutation databases

BioMutaiPLD2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188051 – 933Phospholipase D2Add BLAST933

Post-translational modificationi

Phosphorylated by FGR.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14939.
PaxDbiO14939.
PeptideAtlasiO14939.
PRIDEiO14939.

PTM databases

iPTMnetiO14939.
PhosphoSitePlusiO14939.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000129219.
CleanExiHS_PLD2.
ExpressionAtlasiO14939. baseline and differential.
GenevisibleiO14939. HS.

Organism-specific databases

HPAiHPA013397.

Interactioni

Subunit structurei

Interacts with EGFR (By similarity). Interacts with PIP5K1B.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CFL1P235282EBI-1053996,EBI-352733
GRB2P629934EBI-1053996,EBI-401755

Protein-protein interaction databases

BioGridi111354. 44 interactors.
DIPiDIP-38903N.
IntActiO14939. 4 interactors.
MINTiMINT-141629.
STRINGi9606.ENSP00000263088.

Chemistry databases

BindingDBiO14939.

Structurei

3D structure databases

ProteinModelPortaliO14939.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 195PXPROSITE-ProRule annotationAdd BLAST131
Domaini203 – 311PHAdd BLAST109
Domaini437 – 464PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST28
Domaini751 – 778PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni441 – 788CatalyticAdd BLAST348

Sequence similaritiesi

Belongs to the phospholipase D family.Curated
Contains 1 PH domain.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
GeneTreeiENSGT00390000008356.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
InParanoidiO14939.
KOiK01115.
OMAiHHETLNF.
OrthoDBiEOG091G017Y.
PhylomeDBiO14939.
TreeFamiTF300589.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D1/D2.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 2 hits.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform PLD2A (identifier: O14939-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ
60 70 80 90 100
SLKVHPLVFA PGVPVTAQVV GTERYTSGSK VGTCTLYSVR LTHGDFSWTT
110 120 130 140 150
KKKYRHFQEL HRDLLRHKVL MSLLPLARFA VAYSPARDAG NREMPSLPRA
160 170 180 190 200
GPEGSTRHAA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL
210 220 230 240 250
GRKGLEGMIR KRSGGHRVPG LTCCGRDQVC YRWSKRWLVV KDSFLLYMCL
260 270 280 290 300
ETGAISFVQL FDPGFEVQVG KRSTEARHGV RIDTSHRSLI LKCSSYRQAR
310 320 330 340 350
WWAQEITELA QGPGRDFLQL HRHDSYAPPR PGTLARWFVN GAGYFAAVAD
360 370 380 390 400
AILRAQEEIF ITDWWLSPEV YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL
410 420 430 440 450
LFKEVELALG INSGYSKRAL MLLHPNIKVM RHPDQVTLWA HHEKLLVVDQ
460 470 480 490 500
VVAFLGGLDL AYGRWDDLHY RLTDLGDSSE SAASQPPTPR PDSPATPDLS
510 520 530 540 550
HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGL
560 570 580 590 600
PARDLARHFI QRWNFTKTTK AKYKTPTYPY LLPKSTSTAN QLPFTLPGGQ
610 620 630 640 650
CTTVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG
660 670 680 690 700
RTVLNKVGDE IVDRILKAHK QGWCYRVYVL LPLLPGFEGD ISTGGGNSIQ
710 720 730 740 750
AILHFTYRTL CRGEYSILHR LKAAMGTAWR DYISICGLRT HGELGGHPVS
760 770 780 790 800
ELIYIHSKVL IADDRTVIIG SANINDRSLL GKRDSELAVL IEDTETEPSL
810 820 830 840 850
MNGAEYQAGR FALSLRKHCF GVILGANTRP DLDLRDPICD DFFQLWQDMA
860 870 880 890 900
ESNANIYEQI FRCLPSNATR SLRTLREYVA VEPLATVSPP LARSELTQVQ
910 920 930
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT
Length:933
Mass (Da):105,987
Last modified:February 21, 2001 - v2
Checksum:iF25F6B73B45F57ED
GO
Isoform PLD2C (identifier: O14939-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     337-933: Missing.

Show »
Length:336
Mass (Da):38,252
Checksum:iB88330C3765169E3
GO
Isoform PLD2B (identifier: O14939-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     810-821: RFALSLRKHCFG → S

Show »
Length:922
Mass (Da):104,657
Checksum:iC160953BA88F8F19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16D → G in AAB96656 (Ref. 3) Curated1
Sequence conflicti165Y → C in AAB96656 (Ref. 3) Curated1
Sequence conflicti212R → C in AAB96656 (Ref. 3) Curated1
Sequence conflicti268Q → R in AAB96655 (Ref. 3) Curated1
Sequence conflicti325S → R in AAB96656 (Ref. 3) Curated1
Sequence conflicti445L → P in AAB96656 (Ref. 3) Curated1
Sequence conflicti510D → G in AAB96656 (Ref. 3) Curated1
Sequence conflicti566T → I in AAB96655 (Ref. 3) Curated1
Sequence conflicti784D → G in AAB96656 (Ref. 3) Curated1
Sequence conflicti801 – 802MN → ID in AAC24498 (PubMed:9582313).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051704172R → C.2 PublicationsCorresponds to variant rs2286672dbSNPEnsembl.1
Natural variantiVAR_051705577T → I.2 PublicationsCorresponds to variant rs1052748dbSNPEnsembl.1
Natural variantiVAR_061750632E → G.2 PublicationsCorresponds to variant rs17854914dbSNPEnsembl.1
Natural variantiVAR_051706804A → T.Corresponds to variant rs11545163dbSNPEnsembl.1
Natural variantiVAR_036503807Q → E in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_051707821G → R.Corresponds to variant rs3764897dbSNPEnsembl.1
Natural variantiVAR_059774821G → S.1 PublicationCorresponds to variant rs3764897dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005027337 – 933Missing in isoform PLD2C. 1 PublicationAdd BLAST597
Alternative sequenceiVSP_054769810 – 821RFALS…KHCFG → S in isoform PLD2B. 2 PublicationsAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033850 mRNA. Translation: AAD04197.1.
AF035483 mRNA. Translation: AAC24498.1.
AF038440 mRNA. Translation: AAB96655.1.
AF038441 mRNA. Translation: AAB96656.1.
AC233723 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90405.1.
BC015033 mRNA. Translation: AAH15033.1.
BC056871 mRNA. Translation: AAH56871.1.
CCDSiCCDS11057.1. [O14939-1]
CCDS58507.1. [O14939-4]
RefSeqiNP_001230037.1. NM_001243108.1. [O14939-4]
NP_002654.3. NM_002663.4. [O14939-1]
UniGeneiHs.104519.

Genome annotation databases

EnsembliENST00000263088; ENSP00000263088; ENSG00000129219. [O14939-1]
ENST00000572940; ENSP00000459571; ENSG00000129219. [O14939-4]
GeneIDi5338.
KEGGihsa:5338.
UCSCiuc002fzc.4. human. [O14939-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Phospholipase D entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033850 mRNA. Translation: AAD04197.1.
AF035483 mRNA. Translation: AAC24498.1.
AF038440 mRNA. Translation: AAB96655.1.
AF038441 mRNA. Translation: AAB96656.1.
AC233723 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90405.1.
BC015033 mRNA. Translation: AAH15033.1.
BC056871 mRNA. Translation: AAH56871.1.
CCDSiCCDS11057.1. [O14939-1]
CCDS58507.1. [O14939-4]
RefSeqiNP_001230037.1. NM_001243108.1. [O14939-4]
NP_002654.3. NM_002663.4. [O14939-1]
UniGeneiHs.104519.

3D structure databases

ProteinModelPortaliO14939.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111354. 44 interactors.
DIPiDIP-38903N.
IntActiO14939. 4 interactors.
MINTiMINT-141629.
STRINGi9606.ENSP00000263088.

Chemistry databases

BindingDBiO14939.
ChEMBLiCHEMBL2734.
DrugBankiDB00122. Choline.
GuidetoPHARMACOLOGYi1434.
SwissLipidsiSLP:000000108.

PTM databases

iPTMnetiO14939.
PhosphoSitePlusiO14939.

Polymorphism and mutation databases

BioMutaiPLD2.

Proteomic databases

MaxQBiO14939.
PaxDbiO14939.
PeptideAtlasiO14939.
PRIDEiO14939.

Protocols and materials databases

DNASUi5338.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263088; ENSP00000263088; ENSG00000129219. [O14939-1]
ENST00000572940; ENSP00000459571; ENSG00000129219. [O14939-4]
GeneIDi5338.
KEGGihsa:5338.
UCSCiuc002fzc.4. human. [O14939-1]

Organism-specific databases

CTDi5338.
DisGeNETi5338.
GeneCardsiPLD2.
H-InvDBHIX0013451.
HGNCiHGNC:9068. PLD2.
HPAiHPA013397.
MIMi602384. gene.
neXtProtiNX_O14939.
OpenTargetsiENSG00000129219.
PharmGKBiPA33397.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
GeneTreeiENSGT00390000008356.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
InParanoidiO14939.
KOiK01115.
OMAiHHETLNF.
OrthoDBiEOG091G017Y.
PhylomeDBiO14939.
TreeFamiTF300589.

Enzyme and pathway databases

BioCyciZFISH:HS05256-MONOMER.
BRENDAi3.1.4.4. 2681.
4.2.1.1. 2681.
ReactomeiR-HSA-1483148. Synthesis of PG.
R-HSA-1483166. Synthesis of PA.
R-HSA-2029485. Role of phospholipids in phagocytosis.
SignaLinkiO14939.
SIGNORiO14939.

Miscellaneous databases

ChiTaRSiPLD2. human.
GeneWikiiPLD2.
GenomeRNAii5338.
PROiO14939.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000129219.
CleanExiHS_PLD2.
ExpressionAtlasiO14939. baseline and differential.
GenevisibleiO14939. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D1/D2.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 2 hits.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLD2_HUMAN
AccessioniPrimary (citable) accession number: O14939
Secondary accession number(s): I3L2C9
, O43540, O43579, O43580, Q6PGR0, Q96BY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 2, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.