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Reviewed, UniProtKB/Swiss-Prot O14933 (UB2L6_HUMAN)

Last modified June 16, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ubiquitin/ISG15-conjugating enzyme E2 L6
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase L6
    Ubiquitin carrier protein L6
    UbcH8
    Retinoic acid-induced gene B protein
      Short name=RIG-B
Gene names
Name: UBE2L6
Synonyms: UBCH8
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length153 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Ref.10

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with RNF19A, RNF19B and RNF144B. Ref.11 Ref.12 Ref.13

Tissue specificity

Present in natural killer cells (at protein level). Ref.13

Induction

By interferon beta. Ref.10

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14933-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14933-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 153153Ubiquitin/ISG15-conjugating enzyme E2 L6
PRO_0000082478

Sites

Active site861Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence1 – 6666Missing in isoform 2.
VSP_037344

Secondary structure

.......................... 153
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 16, 2009. Version 4.
Checksum: D79768775344EFAE

FASTA15317,769
        10         20         30         40         50         60 
MMASMRVVKE LEDLQKKPPP YLRNLSSDDA NVLVWHALLL PDQPPYHLKA FNLRISFPPE 

        70         80         90        100        110        120 
YPFKPPMIKF TTKIYHPNVD ENGQICLPII SSENWKPCTK TCQVLEALNV LVNRPNIREP 

       130        140        150 
LRMDLADLLT QNPELFRKNA EEFTLRFGVD RPS 

« Hide

Isoform 2.

Checksum: CD4DEC38615607B1
Show »

FASTA8710,086

References

« Hide 'large scale' references
[1]"Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12."
Ardley H.C., Rose S.A., Tan N., Leek J.P., Markham A.F., Robinson P.A.
Cytogenet. Cell Genet. 89:137-140(2000) [PubMed: 10894956] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[2]"Human retinoic acid induced gene B (RIG-B) mRNA."
Liu T., Mao M., Zhang J., Wu J., Zhang Q., Fu G., Shen Y., Zhou J., Yu Y., Wang Z., Chen S., Chen Z.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Thymus.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[6]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed: 16554811] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[9]"Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity."
Kumar S., Kao W.H., Howley P.M.
J. Biol. Chem. 272:13548-13554(1997) [PubMed: 9153201] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-153 (ISOFORM 1).
[10]"The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein."
Zhao C., Beaudenon S.L., Kelley M.L., Waddell M.B., Yuan W., Schulman B.A., Huibregtse J.M., Krug R.M.
Proc. Natl. Acad. Sci. U.S.A. 101:7578-7582(2004) [PubMed: 15131269] [Abstract]
Cited for: PROTEIN SEQUENCE OF 140-146, FUNCTION IN ISG15 ATTACHMENT, INDUCTION BY INTERFERON BETA.
[11]"A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity."
Niwa J., Ishigaki S., Doyu M., Suzuki T., Tanaka K., Sobue G.
Biochem. Biophys. Res. Commun. 281:706-713(2001) [PubMed: 11237715] [Abstract]
Cited for: INTERACTION WITH RNF19A.
[12]"The p53-inducible E3 ubiquitin ligase p53RFP induces p53-dependent apoptosis."
Huang J., Xu L.-G., Liu T., Zhai Z., Shu H.-B.
FEBS Lett. 580:940-947(2006) [PubMed: 16427630] [Abstract]
Cited for: INTERACTION WITH RNF144B.
[13]"NK lytic-associated molecule, involved in NK cytotoxic function, is an E3 ligase."
Fortier J.M., Kornbluth J.
J. Immunol. 176:6454-6463(2006) [PubMed: 16709802] [Abstract]
Cited for: INTERACTION WITH RNF19B, TISSUE SPECIFICITY.
[14]"Crystal structure of Ubch8."
Mizushima T., Suzuki M., Teshima N., Yamane T., Murata S., Tanaka K.
Submitted (MAR-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-153.
+Additional computationally mapped references.

Cross-references

Sequence databases

AJ243268 expand/collapse EMBL AC list , AJ243269, AJ243270, AJ243271 Genomic DNA. Translation: CAB64566.1. Different initiation.
AF061736 mRNA. Translation: AAD17525.1.
AK315755 mRNA. Translation: BAG38109.1.
CR457126 mRNA. Translation: CAG33407.1.
AK226165 mRNA. No translation available.
AP002893 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73757.1.
CH471076 Genomic DNA. Translation: EAW73759.1.
BC032491 mRNA. Translation: AAH32491.1.
AF031141 mRNA. Translation: AAB86433.1.
IPIIPI00329563.
RefSeqNP_004214.1.
UniGeneHs.425777

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1WZVX-ray2.10A/B1-152[»]
1WZWX-ray2.40A1-152[»]
ModBaseSearch...

Proteomic databases

PRIDEO14933.

Genome annotation databases

EnsemblENSG00000156587. Homo sapiens. [Contig view]
GeneID9246.
KEGGhsa:9246.

Organism-specific databases

GeneCardsGC11M057075.
HGNCHGNC:12490. UBE2L6.
HPAHPA003328.
MIM603890. gene.
PharmGKBPA37139.
GenAtlasSearch...

Phylogenomic databases

HOVERGENO14933.
OMAO14933. EFTLQFG.

Enzyme and pathway databases

BRENDA6.3.2.19. 247.

Gene expression databases

ArrayExpressO14933.
BgeeO14933.
Q8N5D8.
CleanExHS_UBE2L6.
GermOnlineENSG00000156587. Homo sapiens.

Family and domain databases

InterProIPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]
Gene3DG3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
ProDomPD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00212. UBCc. 1 hit.
[Graphical view]
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio34661.
SOURCESearch...

Entry information

Entry nameUB2L6_HUMAN
AccessionPrimary (citable) accession number: O14933
Secondary accession number(s): A6NDM6 expand/collapse secondary AC list , A8MY53, Q8N5D8, Q9UEZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 16, 2009
Last modified: June 16, 2009
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents