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Protein

Ubiquitin/ISG15-conjugating enzyme E2 L6

Gene

UBE2L6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3.3 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ISG15 transferase activity Source: Ensembl
  • ubiquitin binding Source: Reactome
  • ubiquitin-protein transferase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
R-HSA-977225 Amyloid fiber formation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiO14933
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin/ISG15-conjugating enzyme E2 L6 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme L6
Retinoic acid-induced gene B protein
Short name:
RIG-B
UbcH8
Ubiquitin carrier protein L6
Ubiquitin-protein ligase L6
Gene namesi
Name:UBE2L6
Synonyms:UBCH8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000156587.15
HGNCiHGNC:12490 UBE2L6
MIMi603890 gene
neXtProtiNX_O14933

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9246
OpenTargetsiENSG00000156587
PharmGKBiPA37139

Polymorphism and mutation databases

BioMutaiUBE2L6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824781 – 153Ubiquitin/ISG15-conjugating enzyme E2 L6Add BLAST153

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiO14933
MaxQBiO14933
PaxDbiO14933
PeptideAtlasiO14933
PRIDEiO14933

PTM databases

iPTMnetiO14933
PhosphoSitePlusiO14933
SwissPalmiO14933

Expressioni

Tissue specificityi

Present in natural killer cells (at protein level).1 Publication

Inductioni

By IFNB1/IFN-beta.1 Publication

Gene expression databases

BgeeiENSG00000156587
CleanExiHS_UBE2L6
ExpressionAtlasiO14933 baseline and differential
GenevisibleiO14933 HS

Organism-specific databases

HPAiCAB073407
HPA003328

Interactioni

Subunit structurei

Interacts with RNF19A, RNF19B and RNF144B. Interacts with FLT3 (tyrosine phosphorylated).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi11467252 interactors.
DIPiDIP-41477N
IntActiO14933 31 interactors.
MINTiO14933
STRINGi9606.ENSP00000287156

Structurei

Secondary structure

1153
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13
Beta strandi22 – 27Combined sources6
Beta strandi34 – 39Combined sources6
Helixi46 – 48Combined sources3
Beta strandi49 – 56Combined sources8
Turni59 – 62Combined sources4
Beta strandi67 – 72Combined sources6
Beta strandi81 – 83Combined sources3
Helixi88 – 90Combined sources3
Turni92 – 94Combined sources3
Helixi101 – 113Combined sources13
Beta strandi117 – 119Combined sources3
Helixi123 – 131Combined sources9
Helixi133 – 147Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WZVX-ray2.10A/B2-153[»]
1WZWX-ray2.40A2-153[»]
2KJHNMR-A2-153[»]
ProteinModelPortaliO14933
SMRiO14933
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14933

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0422 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiO14933
KOiK04553
OMAiSNENWKP
OrthoDBiEOG091G0QDO
PhylomeDBiO14933
TreeFamiTF313043

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.101 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14933-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMASMRVVKE LEDLQKKPPP YLRNLSSDDA NVLVWHALLL PDQPPYHLKA
60 70 80 90 100
FNLRISFPPE YPFKPPMIKF TTKIYHPNVD ENGQICLPII SSENWKPCTK
110 120 130 140 150
TCQVLEALNV LVNRPNIREP LRMDLADLLT QNPELFRKNA EEFTLRFGVD

RPS
Length:153
Mass (Da):17,769
Last modified:June 16, 2009 - v4
Checksum:iD79768775344EFAE
GO
Isoform 2 (identifier: O14933-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.

Show »
Length:87
Mass (Da):10,086
Checksum:iCD4DEC38615607B1
GO

Sequence cautioni

The sequence CAB64566 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0373441 – 66Missing in isoform 2. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243268
, AJ243269, AJ243270, AJ243271 Genomic DNA Translation: CAB64566.1 Different initiation.
AF061736 mRNA Translation: AAD17525.1
AK315755 mRNA Translation: BAG38109.1
CR457126 mRNA Translation: CAG33407.1
AK226165 mRNA No translation available.
AP002893 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW73757.1
CH471076 Genomic DNA Translation: EAW73759.1
BC032491 mRNA Translation: AAH32491.1
AF031141 mRNA Translation: AAB86433.1
CCDSiCCDS7960.1 [O14933-1]
CCDS7961.1 [O14933-2]
RefSeqiNP_004214.1, NM_004223.4 [O14933-1]
NP_937826.1, NM_198183.2 [O14933-2]
UniGeneiHs.425777

Genome annotation databases

EnsembliENST00000287156; ENSP00000287156; ENSG00000156587 [O14933-1]
ENST00000340573; ENSP00000341980; ENSG00000156587 [O14933-2]
GeneIDi9246
KEGGihsa:9246
UCSCiuc001nkn.3 human [O14933-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUB2L6_HUMAN
AccessioniPrimary (citable) accession number: O14933
Secondary accession number(s): A6NDM6
, A8MY53, Q8N5D8, Q9UEZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 177 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome