O14925 (TIM23_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit Tim23

Gene names

Name:	TIMM23
Synonyms:	TIM23

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Subunit structure	Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50. Interacts directly with TIMM50. The complex interacts with the TIMM44 component of the PAM complex. Ref.6
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the Tim17/Tim22/Tim23 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein metabolic process Traceable author statement. Source: Reactome protein targeting to mitochondrion Traceable author statement. Source: Reactome
Cellular_component	integral to mitochondrial inner membrane Inferred from direct assay Ref.1. Source: BHF-UCL mitochondrial inner membrane presequence translocase complex Inferred from direct assay Ref.1. Source: BHF-UCL mitochondrial intermembrane space Inferred from direct assay Ref.6. Source: MGI
Molecular_function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Mitochondrial import inner membrane translocase subunit Tim23		PRO_0000210302
Regions
	Transmembrane	73 – 93	21	Helical; Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	181 – 197	17	Helical; Potential
Experimental info
	Sequence conflict	27	1	S → P in BAD97051. Ref.3
	Sequence conflict	62	1	Missing in AAH62707. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O14925 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C9A1BBEC703B6838	FASTA	209	21,943
	10 20 30 40 50 60 MEGGGGSGNK TTGGLAGFFG AGGAGYSHAD LAGVPLTGMN PLSPYLNVDP RYLVQDTDEF 70 80 90 100 110 120 ILPTGANKTR GRFELAFFTI GGCCMTGAAF GAMNGLRLGL KETQNMAWSK PRNVQILNMV 130 140 150 160 170 180 TRQGALWANT LGSLALLYSA FGVIIEKTRG AEDDLNTVAA GTMTGMLYKC TGGLRGIARG 190 200 GLTGLTLTSL YALYNNWEHM KGSLLQQSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and structural characterization of the human mitochondrial inner membrane translocase." Bauer M.F., Gempel K., Reichert A.S., Rappold G.A., Lichtner P., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S. J. Mol. Biol. 289:69-82(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of Homo sapiens translocase of inner mitochondrial membrane 23 (Tim23) mRNA." Cao X., Li Q., Jin K., Liu L., Dai S., Wu G., Dong C. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis.
[6]	"Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death." Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A., Fernandes-Alnemri T., Alnemri E.S. J. Biol. Chem. 279:24813-24825(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TIMM50.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030162 mRNA. Translation: AAB84060.1. AY442341 mRNA. Translation: AAR14723.1. AK223331 mRNA. Translation: BAD97051.1. AL450342 Genomic DNA. Translation: CAI14480.1. BC062707 mRNA. Translation: AAH62707.1. BC066951 mRNA. Translation: AAH66951.1.
IPI	IPI00007309.
RefSeq	NP_006318.1. NM_006327.3.
UniGene	Hs.499594. Hs.524308. Hs.661964.
3D structure databases
ProteinModelPortal	O14925.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14925. 2 interactions.
STRING	9606.ENSP00000260867.
PTM databases
PhosphoSite	O14925.
Proteomic databases
PaxDb	O14925.
PRIDE	O14925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000260867; ENSP00000260867; ENSG00000138297.
GeneID	100287932.
KEGG	hsa:100287932.
UCSC	uc001jin.3. human.
Organism-specific databases
CTD	100287932.
GeneCards	GC10M051591.
HGNC	HGNC:17312. TIMM23.
HPA	HPA031408. HPA047180.
MIM	605034. gene.
neXtProt	NX_O14925.
PharmGKB	PA38227.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5596.
HOGENOM	HOG000166435.
HOVERGEN	HBG057526.
InParanoid	O14925.
OMA	RFELAFS.
OrthoDB	EOG4GTKF4.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	O14925.
Bgee	O14925.
CleanEx	HS_TIMM23.
Genevestigator	O14925.
GermOnline	ENSG00000138297. Homo sapiens.
Family and domain databases
InterPro	IPR003397. Tim17/Tim22/Tim23/PMP24. IPR005681. Tim23. [Graphical view]
Pfam	PF02466. Tim17. 1 hit. [Graphical view]
TIGRFAMs	TIGR00983. 3a0801s02tim23. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	100287932.
NextBio	20777681.
SOURCE	Search...

Entry information

Entry name

TIM23_HUMAN

Accession

Primary (citable) accession number: O14925
Secondary accession number(s): Q53FF8, Q5T1E6, Q6P5S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.