O14907 (TX1B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tax1-binding protein 3

Alternative name(s):
Glutaminase-interacting protein 3
Tax interaction protein 1
Short name=TIP-1
Tax-interacting protein 1

Gene names

Name:	TAX1BP3
Synonyms:	TIP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate a number of protein-protein interactions by competing for PDZ domain binding sites. Binds CTNNB1 and may thereby act as an inhibitor of the Wnt signaling pathway. Competes with LIN7A for KCNJ4 binding, and thereby promotes KCNJ4 internalization. May play a role in the Rho signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6. Ref.8 Ref.9 Ref.13
Subunit structure	Interacts (via its PDZ domain) with GLS2. Interacts (via its PDZ domain) with RTKN (via the C-terminal region); this interaction facilitates Rho-mediated activation of the FOS serum response element (SRE). Interacts (via its PDZ domain) with CTNNB1; this interaction inhibits the transcriptional activity of CTNNB1. Interacts with HTLV-1 TAX protein. Interacts (via PDZ domain) with ARHGEF16. Interacts (via PDZ domain) with KCNJ4 (via C-terminus). Competes with LIN7A for KCNJ4 binding. Ref.1 Ref.3 Ref.8 Ref.9 Ref.13 Ref.14 Ref.15 Ref.17
Subcellular location	Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Recruited to the cell membrane by interaction with membrane proteins. Ref.8 Ref.9
Tissue specificity	Ubiquitous. Detected in brain, heart, kidney, lung, small intestine and skeletal muscle. Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes. Ref.8 Ref.9
Sequence similarities	Contains 1 PDZ (DHR) domain.
Sequence caution	The sequence AAF43104.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from direct assay Ref.8. Source: UniProtKB Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW activation of Cdc42 GTPase activity Inferred from direct assay Ref.13. Source: BHF-UCL negative regulation of Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of protein localization to cell surface Inferred from direct assay Ref.9. Source: UniProtKB
Cellular_component	cytoplasm Non-traceable author statement Ref.8. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 124

124

Tax1-binding protein 3

PRO_0000233943

Regions

Domain

15 – 112

98

PDZ

Amino acid modifications

Modified residue

61

1

Phosphoserine Ref.11

Experimental info

Mutagenesis

20

1

K → A: Abolishes interaction with KCNJ4. Ref.9

Mutagenesis

90

1

H → A: Abolishes interaction with KCNJ4. Ref.9

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

124

Helix Strand Turn

Details...

Beta strand

9 – 11

3

Beta strand

12 – 19

8

Beta strand

21 – 23

3

Beta strand

26 – 28

3

Beta strand

30 – 35

6

Beta strand

37 – 39

3

Helix

41 – 43

3

Turn

45 – 47

3

Beta strand

48 – 50

3

Beta strand

54 – 60

7

Beta strand

62 – 64

3

Helix

65 – 69

5

Beta strand

76 – 80

5

Beta strand

83 – 85

3

Helix

90 – 97

8

Beta strand

98 – 101

4

Beta strand

103 – 111

9

Beta strand

115 – 118

4

Turn

121 – 123

3

Sequences

	Sequence		Length	Mass (Da)
	O14907 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: FF0BCDF475F682CD	FASTA	124	13,735
	10 20 30 40 50 60 MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV 70 80 90 100 110 120 SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The C-terminus of the HTLV-1 Tax oncoprotein mediates interaction with the PDZ domain of cellular proteins." Rousset R., Fabre S., Desbois C., Bantignies F., Jalinot P. Oncogene 16:643-654(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH HTLV-1 TAX. Tissue: Peripheral blood lymphocyte.
[2]	"The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion." Touchman J.W., Anikster Y., Dietrich N.L., Maduro V.V.B., McDowell G., Shotelersuk V., Bouffard G.G., Beckstrom-Sternberg S.M., Gahl W.A., Green E.D. Genome Res. 10:165-173(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins." Olalla L., Aledo J.C., Bannenberg G., Marquez J. FEBS Lett. 488:116-122(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH GLS2. Tissue: Brain.
[4]	"hPWP1-interacting proteins 2 and 11 (Tax-interacting protein 1)." Honore B. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[8]	"The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element." Reynaud C., Fabre S., Jalinot P. J. Biol. Chem. 275:33962-33968(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RTKN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]	"TIP-1 has PDZ scaffold antagonist activity." Alewine C., Olsen O., Wade J.B., Welling P.A. Mol. Biol. Cell 17:4200-4211(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH KCNJ4, MUTAGENESIS OF LYS-20 AND HIS-90, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42." Oliver A.W., He X., Borthwick K., Donne A.J., Hampson L., Hampson I.N. Br. J. Cancer 104:324-331(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARHGEF16.
[14]	"Solution structure of the human Tax-interacting protein-1." Durney M.A., Birrane G., Anklin C., Soni A., Ladias J.A. J. Biomol. NMR 45:329-334(2009) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, INTERACTION WITH KCNJ4.
[15]	"Molecular mechanism of inward rectifier potassium channel 2.3 regulation by tax-interacting protein-1." Yan X., Zhou H., Zhang J., Shi C., Xie X., Wu Y., Tian C., Shen Y., Long J. J. Mol. Biol. 392:967-976(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS), INTERACTION WITH KCNJ4.
[16]	"The structure of the PDZ domain of TAX1BP." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 13-124.
[17]	"Promiscuous binding at the crossroads of numerous cancer pathways: insight from the binding of glutaminase interacting protein with glutaminase L." Zoetewey D.L., Ovee M., Banerjee M., Bhaskaran R., Mohanty S. Biochemistry 50:3528-3539(2011) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR IN COMPLEX WITH GLS2, INTERACTION WITH GLS2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF028823 mRNA. Translation: AAB84248.2.
AF168787 Genomic DNA. Translation: AAF43104.1. Different initiation.
AF234997 mRNA. Translation: AAG44368.1.
AF277318 mRNA. Translation: AAK69111.1.
AK315408 mRNA. Translation: BAG37800.1.
CH471108 Genomic DNA. Translation: EAW90491.1.
CH471108 Genomic DNA. Translation: EAW90492.1.
BC023980 mRNA. Translation: AAH23980.1.

IPI

IPI00005585.

RefSeq

NP_001191627.1. NM_001204698.1.
NP_055419.1. NM_014604.3.

UniGene

Hs.12956.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KG2	NMR	-	A	2-124	[»]
2L4S	NMR	-	A	1-124	[»]
2L4T	NMR	-	A	1-124	[»]
2VZ5	X-ray	1.74	A	13-124	[»]
3GJ9	X-ray	2.80	A/B	1-124	[»]
3SFJ	X-ray	1.24	A/C	9-112	[»]
4E3B	X-ray	1.50	A/B	11-112	[»]

ProteinModelPortal

O14907.

ModBase

Search...

Protein-protein interaction databases

IntAct

O14907. 8 interactions.

MINT

MINT-142501.

STRING

9606.ENSP00000225525.

PTM databases

PhosphoSite

O14907.

2D gel databases

UCD-2DPAGE

O14907.

Proteomic databases

PaxDb

O14907.

PRIDE

O14907.

Protocols and materials databases

DNASU

30851.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000225525; ENSP00000225525; ENSG00000213977.

GeneID

30851.

KEGG

hsa:30851.

UCSC

uc002fwc.3. human.

Organism-specific databases

CTD

30851.

GeneCards

GC17M003566.

HGNC

HGNC:30684. TAX1BP3.

neXtProt

NX_O14907.

PharmGKB

PA134950693.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG236405.

HOGENOM

HOG000015753.

HOVERGEN

HBG052814.

InParanoid

O14907.

OMA

LRQGDNL.

OrthoDB

EOG4MKNHP.

PhylomeDB

O14907.

Enzyme and pathway databases

BRENDA

3.5.1.2. 2681.

Gene expression databases

Bgee

O14907.

CleanEx

HS_TAX1BP3.

Genevestigator

O14907.

GermOnline

ENSG00000083454. Homo sapiens.

Family and domain databases

InterPro

IPR001478. PDZ.
IPR017268. Tax1-binding_p3.
[Graphical view]

Pfam

PF00595. PDZ. 1 hit.
[Graphical view]

PIRSF

PIRSF037712. Tax1-binding_p3. 1 hit.

SMART

SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.

PROSITE

PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TAX1BP3. human.

EvolutionaryTrace

O14907.

GenomeRNAi

30851.

NextBio

52992.

Entry information

Entry name

TX1B3_HUMAN

Accession

Primary (citable) accession number: O14907
Secondary accession number(s): B2RD53, D3DTJ6, Q7LCQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.