O14907 (TX1B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tax1-binding protein 3

Alternative name(s):
Glutaminase-interacting protein 3
Tax interaction protein 1
Short name=TIP-1

Gene names

Name:	TAX1BP3
Synonyms:	TIP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the Rho signaling pathway. May act as an inhibitor of the Wnt signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6. Ref.8 Ref.10 UniProtKB Q9DBG9
Subunit structure	Interacts (via its PDZ domain) with GLS2. Interacts (via its PDZ domain) with RTKN (via the C-terminal region); this interaction facilitates Rho-mediated activation of the FOS serum response element (SRE). Interacts (via its PDZ domain) with CTNNB1; this interaction inhibits the transcriptional activity of CTNNB1. Interacts with HTLV-1 TAX protein. Interacts (via PDZ domain) with ARHGEF16. Ref.1 Ref.3 Ref.8 Ref.10
Subcellular location	Cytoplasm. Nucleus Ref.8.
Tissue specificity	Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes. Ref.8
Sequence similarities	Contains 1 PDZ (DHR) domain.
Sequence caution	The sequence AAF43104.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Inferred from direct assay Ref.8. Source: UniProtKB Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW activation of Cdc42 GTPase activity Inferred from direct assay Ref.10. Source: BHF-UCL negative regulation of Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein localization at cell surface Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Non-traceable author statement Ref.8. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein C-terminus binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 124

124

Tax1-binding protein 3

PRO_0000233943

Regions

Domain

15 – 112

98

PDZ

Amino acid modifications

Modified residue

61

1

Phosphoserine Ref.9

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

124

Helix Strand Turn

Details...

Beta strand

13 – 19

7

Helix

41 – 43

3

Turn

66 – 68

3

Beta strand

76 – 80

5

Helix

90 – 97

8

Beta strand

103 – 111

9

Sequences

	Sequence		Length	Mass (Da)
	O14907 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: FF0BCDF475F682CD	FASTA	124	13,735
	10 20 30 40 50 60 MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV 70 80 90 100 110 120 SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The C-terminus of the HTLV-1 Tax oncoprotein mediates interaction with the PDZ domain of cellular proteins." Rousset R., Fabre S., Desbois C., Bantignies F., Jalinot P. Oncogene 16:643-654(1998) [PubMed: 9482110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH HTLV-1 TAX. Tissue: Peripheral blood lymphocyte.
[2]	"The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion." Touchman J.W., Anikster Y., Dietrich N.L., Maduro V.V.B., McDowell G., Shotelersuk V., Bouffard G.G., Beckstrom-Sternberg S.M., Gahl W.A., Green E.D. Genome Res. 10:165-173(2000) [PubMed: 10673275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins." Olalla L., Aledo J.C., Bannenberg G., Marquez J. FEBS Lett. 488:116-122(2001) [PubMed: 11163757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH GLS2. Tissue: Brain.
[4]	"hPWP1-interacting proteins 2 and 11 (Tax-interacting protein 1)." Honore B. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[8]	"The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element." Reynaud C., Fabre S., Jalinot P. J. Biol. Chem. 275:33962-33968(2000) [PubMed: 10940294] [Abstract] Cited for: FUNCTION, INTERACTION WITH RTKN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42." Oliver A.W., He X., Borthwick K., Donne A.J., Hampson L., Hampson I.N. Br. J. Cancer 104:324-331(2011) [PubMed: 21139582] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARHGEF16.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF028823 mRNA. Translation: AAB84248.2.
AF168787 Genomic DNA. Translation: AAF43104.1. Different initiation.
AF234997 mRNA. Translation: AAG44368.1.
AF277318 mRNA. Translation: AAK69111.1.
AK315408 mRNA. Translation: BAG37800.1.
CH471108 Genomic DNA. Translation: EAW90491.1.
CH471108 Genomic DNA. Translation: EAW90492.1.
BC023980 mRNA. Translation: AAH23980.1.

IPI

IPI00005585.

RefSeq

NP_001191627.1. NM_001204698.1.
NP_055419.1. NM_014604.3.

UniGene

Hs.12956.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KG2	NMR	-	A	2-124	[»]
2L4S	NMR	-	A	1-124	[»]
2L4T	NMR	-	A	1-124	[»]
2VZ5	X-ray	1.74	A	13-124	[»]
3GJ9	X-ray	2.80	A/B	1-124	[»]

ProteinModelPortal

O14907.

SMR

O14907. Positions 1-124.

ModBase

Search...

Protein-protein interaction databases

IntAct

O14907. 8 interactions.

MINT

MINT-142501.

STRING

O14907.

PTM databases

PhosphoSite

O14907.

2D gel databases

UCD-2DPAGE

O14907.

Proteomic databases

PRIDE

O14907.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000225525; ENSP00000225525; ENSG00000213977.

GeneID

30851.

KEGG

hsa:30851.

UCSC

uc002fwc.1. human.

Organism-specific databases

CTD

30851.

GeneCards

GC17M003566.

H-InvDB

HIX0013429.

HGNC

HGNC:30684. TAX1BP3.

neXtProt

NX_O14907.

PharmGKB

PA134950693.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG17348.

GeneTree

ENSGT00390000002877.

HOGENOM

HBG382462.

HOVERGEN

HBG052814.

InParanoid

O14907.

OMA

LRQGDNL.

OrthoDB

EOG4MKNHP.

PhylomeDB

O14907.

Enzyme and pathway databases

BRENDA

3.5.1.2. 2681.

Gene expression databases

ArrayExpress

O14907.

Bgee

O14907.

CleanEx

HS_TAX1BP3.

Genevestigator

O14907.

GermOnline

ENSG00000083454. Homo sapiens.

Family and domain databases

InterPro

IPR001478. PDZ/DHR/GLGF.
IPR017268. Tax1-binding_p3.
[Graphical view]

Pfam

PF00595. PDZ. 1 hit.
[Graphical view]

PIRSF

PIRSF037712. Tax1-binding_p3. 1 hit.

SMART

SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.

PROSITE

PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

52992.

Entry information

Entry name

TX1B3_HUMAN

Accession

Primary (citable) accession number: O14907
Secondary accession number(s): B2RD53, D3DTJ6, Q7LCQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.