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O14907 (TX1B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tax1-binding protein 3
Alternative name(s):
Glutaminase-interacting protein 3
Tax interaction protein 1
Short name=TIP-1
Tax-interacting protein 1
Gene names
Name:TAX1BP3
Synonyms:TIP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length124 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May regulate a number of protein-protein interactions by competing for PDZ domain binding sites. Binds CTNNB1 and may thereby act as an inhibitor of the Wnt signaling pathway. Competes with LIN7A for KCNJ4 binding, and thereby promotes KCNJ4 internalization. May play a role in the Rho signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6. Ref.8 Ref.9 Ref.13

Subunit structure

Interacts (via its PDZ domain) with GLS2. Interacts (via its PDZ domain) with RTKN (via the C-terminal region); this interaction facilitates Rho-mediated activation of the FOS serum response element (SRE). Interacts (via its PDZ domain) with CTNNB1; this interaction inhibits the transcriptional activity of CTNNB1. Interacts with HTLV-1 TAX protein. Interacts (via PDZ domain) with ARHGEF16. Interacts (via PDZ domain) with KCNJ4 (via C-terminus). Competes with LIN7A for KCNJ4 binding. Ref.1 Ref.3 Ref.8 Ref.9 Ref.13 Ref.16 Ref.17 Ref.19

Subcellular location

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Recruited to the cell membrane by interaction with membrane proteins. Ref.8 Ref.9

Tissue specificity

Ubiquitous. Detected in brain, heart, kidney, lung, small intestine and skeletal muscle. Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes. Ref.8 Ref.9

Sequence similarities

Contains 1 PDZ (DHR) domain.

Sequence caution

The sequence AAF43104.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

E6P031262EBI-723259,EBI-1177242From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.14
Chain2 – 124123Tax1-binding protein 3
PRO_0000233943

Regions

Domain15 – 11298PDZ

Amino acid modifications

Modified residue21N-acetylserine Ref.14 Ref.15
Modified residue611Phosphoserine Ref.11

Experimental info

Mutagenesis201K → A: Abolishes interaction with KCNJ4. Ref.9
Mutagenesis901H → A: Abolishes interaction with KCNJ4. Ref.9

Secondary structure

................................... 124
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O14907 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: FF0BCDF475F682CD

FASTA12413,735
        10         20         30         40         50         60 
MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV 

        70         80         90        100        110        120 
SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ 


SMLS 

« Hide

References

« Hide 'large scale' references
[1]"The C-terminus of the HTLV-1 Tax oncoprotein mediates interaction with the PDZ domain of cellular proteins."
Rousset R., Fabre S., Desbois C., Bantignies F., Jalinot P.
Oncogene 16:643-654(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH HTLV-1 TAX.
Tissue: Peripheral blood lymphocyte.
[2]"The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion."
Touchman J.W., Anikster Y., Dietrich N.L., Maduro V.V.B., McDowell G., Shotelersuk V., Bouffard G.G., Beckstrom-Sternberg S.M., Gahl W.A., Green E.D.
Genome Res. 10:165-173(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins."
Olalla L., Aledo J.C., Bannenberg G., Marquez J.
FEBS Lett. 488:116-122(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH GLS2.
Tissue: Brain.
[4]"hPWP1-interacting proteins 2 and 11 (Tax-interacting protein 1)."
Honore B.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Tongue.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[8]"The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element."
Reynaud C., Fabre S., Jalinot P.
J. Biol. Chem. 275:33962-33968(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RTKN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]"TIP-1 has PDZ scaffold antagonist activity."
Alewine C., Olsen O., Wade J.B., Welling P.A.
Mol. Biol. Cell 17:4200-4211(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KCNJ4, MUTAGENESIS OF LYS-20 AND HIS-90, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42."
Oliver A.W., He X., Borthwick K., Donne A.J., Hampson L., Hampson I.N.
Br. J. Cancer 104:324-331(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ARHGEF16.
[14]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Solution structure of the human Tax-interacting protein-1."
Durney M.A., Birrane G., Anklin C., Soni A., Ladias J.A.
J. Biomol. NMR 45:329-334(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, INTERACTION WITH KCNJ4.
[17]"Molecular mechanism of inward rectifier potassium channel 2.3 regulation by tax-interacting protein-1."
Yan X., Zhou H., Zhang J., Shi C., Xie X., Wu Y., Tian C., Shen Y., Long J.
J. Mol. Biol. 392:967-976(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS), INTERACTION WITH KCNJ4.
[18]"The structure of the PDZ domain of TAX1BP."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 13-124.
[19]"Promiscuous binding at the crossroads of numerous cancer pathways: insight from the binding of glutaminase interacting protein with glutaminase L."
Zoetewey D.L., Ovee M., Banerjee M., Bhaskaran R., Mohanty S.
Biochemistry 50:3528-3539(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR IN COMPLEX WITH GLS2, INTERACTION WITH GLS2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF028823 mRNA. Translation: AAB84248.2.
AF168787 Genomic DNA. Translation: AAF43104.1. Different initiation.
AF234997 mRNA. Translation: AAG44368.1.
AF277318 mRNA. Translation: AAK69111.1.
AK315408 mRNA. Translation: BAG37800.1.
CH471108 Genomic DNA. Translation: EAW90491.1.
CH471108 Genomic DNA. Translation: EAW90492.1.
BC023980 mRNA. Translation: AAH23980.1.
RefSeqNP_001191627.1. NM_001204698.1.
NP_055419.1. NM_014604.3.
UniGeneHs.12956.
Hs.731607.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KG2NMR-A2-124[»]
2L4SNMR-A1-124[»]
2L4TNMR-A1-124[»]
2VZ5X-ray1.74A13-124[»]
3GJ9X-ray2.80A/B1-124[»]
3SFJX-ray1.24A/C9-112[»]
4E3BX-ray1.50A/B11-112[»]
ProteinModelPortalO14907.
SMRO14907. Positions 9-112.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119061. 15 interactions.
IntActO14907. 10 interactions.
MINTMINT-142501.
STRING9606.ENSP00000225525.

PTM databases

PhosphoSiteO14907.

2D gel databases

UCD-2DPAGEO14907.

Proteomic databases

PaxDbO14907.
PRIDEO14907.

Protocols and materials databases

DNASU30851.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000225525; ENSP00000225525; ENSG00000213977.
GeneID30851.
KEGGhsa:30851.
UCSCuc002fwc.3. human.

Organism-specific databases

CTD30851.
GeneCardsGC17M003566.
HGNCHGNC:30684. TAX1BP3.
neXtProtNX_O14907.
PharmGKBPA134950693.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG236405.
HOGENOMHOG000015753.
HOVERGENHBG052814.
InParanoidO14907.
OMAAVQQSMM.
OrthoDBEOG7S4X7S.
PhylomeDBO14907.
TreeFamTF318964.

Enzyme and pathway databases

BRENDA3.5.1.2. 2681.

Gene expression databases

BgeeO14907.
CleanExHS_TAX1BP3.
GenevestigatorO14907.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
IPR017268. Tax1-binding_p3.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
[Graphical view]
PIRSFPIRSF037712. Tax1-binding_p3. 1 hit.
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTAX1BP3. human.
EvolutionaryTraceO14907.
GeneWikiTAX1BP3.
GenomeRNAi30851.
NextBio52992.
PROO14907.

Entry information

Entry nameTX1B3_HUMAN
AccessionPrimary (citable) accession number: O14907
Secondary accession number(s): B2RD53, D3DTJ6, Q7LCQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM