O14880 (MGST3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microsomal glutathione S-transferase 3
Short name=Microsomal GST-3
EC=2.5.1.18

Alternative name(s):
Microsomal GST-III

Gene names

Name:

MGST3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Also functions as a glutathione peroxidase.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential. Microsome membrane; Multi-pass membrane protein Potential. Microsome membrane; Lipid-anchor Potential.
Tissue specificity	Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
Sequence similarities	Belongs to the MAPEG family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from Biological aspect of Ancestor. Source: RefGenome lipid metabolic process Traceable author statement Ref.1. Source: ProtInc signal transduction Traceable author statement Ref.1. Source: ProtInc xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	glutathione peroxidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome glutathione transferase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Microsomal glutathione S-transferase 3		PRO_0000217741
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
Amino acid modifications
	Lipidation	150	1	S-palmitoyl cysteine Ref.9
	Lipidation	151	1	S-palmitoyl cysteine Ref.9
Natural variations
	Natural variant	15	1	G → C. Corresponds to variant rs1802087 [ dbSNP | Ensembl ].		VAR_012061
	Natural variant	48	1	P → S. Corresponds to variant rs1802088 [ dbSNP | Ensembl ].		VAR_012062
Experimental info
	Mutagenesis	150	1	C → S: Abolishes S-acylation; when associated with S-151. Ref.9
	Mutagenesis	151	1	C → S: Abolishes S-acylation; when associated with S-150. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	O14880 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B721527247415E78	FASTA	152	16,516
	10 20 30 40 50 60 MAVLSKEYGF VLLTGAASFI MVAHLAINVS KARKKYKVEY PIMYSTDPEN GHIFNCIQRA 70 80 90 100 110 120 HQNTLEVYPP FLFFLAVGGV YHPRIASGLG LAWIVGRVLY AYGYYTGEPS KRSRGALGSI 130 140 150 ALLGLVGTTV CSAFQHLGWV KSGLGSGPKC CH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel microsomal enzyme with glutathione-dependent transferase and peroxidase activities." Jakobsson P.-J., Mancini J.A., Riendeau D., Ford-Hutchinson A.W. J. Biol. Chem. 272:22934-22939(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIEHS SNPs program Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Lung.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Site-specific analysis of protein S-acylation by resin-assisted capture." Forrester M.T., Hess D.T., Thompson J.W., Hultman R., Moseley M.A., Stamler J.S., Casey P.J. J. Lipid Res. 52:393-398(2011) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-150 AND CYS-151, PALMITOYLATION AT CYS-150 AND CYS-151.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF026977 mRNA. Translation: AAB82609.1. CR450359 mRNA. Translation: CAG29355.1. AY388493 Genomic DNA. Translation: AAQ81301.1. AK312103 mRNA. Translation: BAG35039.1. AL451074 Genomic DNA. Translation: CAH74057.1. CH471067 Genomic DNA. Translation: EAW90752.1. BC000505 mRNA. Translation: AAH00505.1. BC003034 mRNA. Translation: AAH03034.1. BC005964 mRNA. Translation: AAH05964.1.
IPI	IPI00024266.
RefSeq	NP_004519.1. NM_004528.3.
UniGene	Hs.191734. Hs.732686.
3D structure databases
ProteinModelPortal	O14880.
SMR	O14880. Positions 11-137.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14880. 2 interactions.
STRING	9606.ENSP00000356859.
PTM databases
PhosphoSite	O14880.
Proteomic databases
PaxDb	O14880.
PRIDE	O14880.
Protocols and materials databases
DNASU	4259.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367884; ENSP00000356859; ENSG00000143198. ENST00000367889; ENSP00000356864; ENSG00000143198.
GeneID	4259.
KEGG	hsa:4259.
UCSC	uc001gdf.3. human.
Organism-specific databases
CTD	4259.
GeneCards	GC01P165600.
HGNC	HGNC:7064. MGST3.
MIM	604564. gene.
neXtProt	NX_O14880.
PharmGKB	PA30793.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG84007.
HOGENOM	HOG000116372.
HOVERGEN	HBG052472.
InParanoid	O14880.
KO	K00799.
OrthoDB	EOG4FN4JZ.
PhylomeDB	O14880.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O14880.
Bgee	O14880.
CleanEx	HS_MGST3.
Genevestigator	O14880.
GermOnline	ENSG00000143198. Homo sapiens.
Family and domain databases
Gene3D	1.20.120.550. 1 hit.
InterPro	IPR023352. MAPEG-like_dom. IPR001129. Membr-assoc_MAPEG. [Graphical view]
Pfam	PF01124. MAPEG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1743186.
ChiTaRS	MGST3. human.
DrugBank	DB00143. Glutathione.
GenomeRNAi	4259.
NextBio	16801.
SOURCE	Search...

Entry information

Entry name

MGST3_HUMAN

Accession

Primary (citable) accession number: O14880
Secondary accession number(s): B2R592, Q6ICN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.