ID IFIT3_HUMAN Reviewed; 490 AA. AC O14879; Q99634; Q9BSK7; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1998, sequence version 1. DT 24-JAN-2024, entry version 199. DE RecName: Full=Interferon-induced protein with tetratricopeptide repeats 3; DE Short=IFIT-3; DE AltName: Full=CIG49; DE AltName: Full=ISG-60; DE AltName: Full=Interferon-induced 60 kDa protein; DE Short=IFI-60K; DE AltName: Full=Interferon-induced protein with tetratricopeptide repeats 4; DE Short=IFIT-4; DE AltName: Full=Retinoic acid-induced gene G protein; DE Short=P60; DE Short=RIG-G; GN Name=IFIT3; Synonyms=CIG-49, IFI60, IFIT4, ISG60; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Foreskin; RX PubMed=9391139; DOI=10.1073/pnas.94.25.13985; RA Zhu H., Cong J.-P., Shenk T.; RT "Use of differential display analysis to assess the effect of human RT cytomegalovirus infection on the accumulation of cellular RNAs: induction RT of interferon-responsive RNAs."; RL Proc. Natl. Acad. Sci. U.S.A. 94:13985-13990(1997). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=9828129; DOI=10.1006/geno.1998.5555; RA de Veer M.J., Sim H., Whisstock J.C., Devenish R.J., Ralph S.J.; RT "IFI60/ISG60/IFIT4, a new member of the human IFI54/IFIT2 family of RT interferon-stimulated genes."; RL Genomics 54:267-277(1998). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA]. RA Yu M., Tong J., Mao M., Chen S., Chen Z.; RT "RIG-G, a novel gene induced by ATRA in acute promyelocytic leukemia cells, RT is a new member of the ISG family."; RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., RA Phelan M., Farmer A.; RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Skin; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [6] RP FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH COPS5. RX PubMed=17050680; DOI=10.1073/pnas.0607830103; RA Xiao S., Li D., Zhu H.Q., Song M.G., Pan X.R., Jia P.M., Peng L.L., RA Dou A.X., Chen G.Q., Chen S.J., Chen Z., Tong J.H.; RT "RIG-G as a key mediator of the antiproliferative activity of interferon- RT related pathways through enhancing p21 and p27 proteins."; RL Proc. Natl. Acad. Sci. U.S.A. 103:16448-16453(2006). RN [7] RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=18706081; DOI=10.1186/ar2475; RA Huang X., Shen N., Bao C., Gu Y., Wu L., Chen S.; RT "Interferon-induced protein IFIT4 is associated with systemic lupus RT erythematosus and promotes differentiation of monocytes into dendritic RT cell-like cells."; RL Arthritis Res. Ther. 10:R91-R91(2008). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203 AND SER-237, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007; RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., RA Greff Z., Keri G., Stemmann O., Mann M.; RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the RT kinome across the cell cycle."; RL Mol. Cell 31:438-448(2008). RN [9] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203 AND SER-478, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=18669648; DOI=10.1073/pnas.0805139105; RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., RA Elledge S.J., Gygi S.P.; RT "A quantitative atlas of mitotic phosphorylation."; RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). RN [10] RP FUNCTION. RX PubMed=20686046; DOI=10.1128/jvi.00818-10; RA Schmeisser H., Mejido J., Balinsky C.A., Morrow A.N., Clark C.R., Zhao T., RA Zoon K.C.; RT "Identification of alpha interferon-induced genes associated with antiviral RT activity in Daudi cells and characterization of IFIT3 as a novel antiviral RT gene."; RL J. Virol. 84:10671-10680(2010). RN [11] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=20068231; DOI=10.1126/scisignal.2000475; RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.; RT "Quantitative phosphoproteomics reveals widespread full phosphorylation RT site occupancy during mitosis."; RL Sci. Signal. 3:RA3-RA3(2010). RN [12] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21269460; DOI=10.1186/1752-0509-5-17; RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., RA Bennett K.L., Superti-Furga G., Colinge J.; RT "Initial characterization of the human central proteome."; RL BMC Syst. Biol. 5:17-17(2011). RN [13] RP FUNCTION, AND INTERACTION WITH IFIT2. RX PubMed=21190939; DOI=10.1074/jbc.m110.207068; RA Stawowczyk M., Van Scoy S., Kumar K.P., Reich N.C.; RT "The interferon stimulated gene 54 promotes apoptosis."; RL J. Biol. Chem. 286:7257-7266(2011). RN [14] RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH MAVS; TBK1; TRAF6 AND RP RIGI. RX PubMed=21813773; DOI=10.4049/jimmunol.1100963; RA Liu X.Y., Chen W., Wei B., Shan Y.F., Wang C.; RT "IFN-induced TPR protein IFIT3 potentiates antiviral signaling by bridging RT MAVS and TBK1."; RL J. Immunol. 187:2559-2568(2011). RN [15] RP REVIEW. RX PubMed=20950130; DOI=10.1089/jir.2010.0101; RA Fensterl V., Sen G.C.; RT "The ISG56/IFIT1 gene family."; RL J. Interferon Cytokine Res. 31:71-78(2011). RN [16] RP FUNCTION, AND INTERACTION WITH IFIT1 AND IFIT2. RX PubMed=21642987; DOI=10.1038/ni.2048; RA Pichlmair A., Lassnig C., Eberle C.A., Gorna M.W., Baumann C.L., RA Burkard T.R., Buerckstuemmer T., Stefanovic A., Krieger S., Bennett K.L., RA Ruelicke T., Weber F., Colinge J., Mueller M., Superti-Furga G.; RT "IFIT1 is an antiviral protein that recognizes 5'-triphosphate RNA."; RL Nat. Immunol. 12:624-630(2011). RN [17] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014; RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., RA Ye M., Zou H.; RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver RT phosphoproteome."; RL J. Proteomics 96:253-262(2014). CC -!- FUNCTION: IFN-induced antiviral protein which acts as an inhibitor of CC cellular as well as viral processes, cell migration, proliferation, CC signaling, and viral replication. Enhances MAVS-mediated host antiviral CC responses by serving as an adapter bridging TBK1 to MAVS which leads to CC the activation of TBK1 and phosphorylation of IRF3 and phosphorylated CC IRF3 translocates into nucleus to promote antiviral gene transcription. CC Exhibits an antiproliferative activity via the up-regulation of cell CC cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CC CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in CC the nucleus and exports it to the cytoplasm for ubiquitin-dependent CC degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby CC increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 CC by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively CC regulate the apoptotic effects of IFIT2. {ECO:0000269|PubMed:17050680, CC ECO:0000269|PubMed:20686046, ECO:0000269|PubMed:21190939, CC ECO:0000269|PubMed:21642987, ECO:0000269|PubMed:21813773}. CC -!- SUBUNIT: Component of an interferon-dependent multiprotein complex, at CC least composed of IFIT1, IFIT2 and IFIT3 (PubMed:21642987). Interacts CC with IFIT1 and IFIT2 (PubMed:21190939, PubMed:21642987). Interacts (via CC N-terminus) with MAVS, TBK1, TRAF6 and RIGI (PubMed:21813773). CC Interacts with COPS5 (PubMed:17050680). {ECO:0000269|PubMed:17050680, CC ECO:0000269|PubMed:21190939, ECO:0000269|PubMed:21642987, CC ECO:0000269|PubMed:21813773}. CC -!- INTERACTION: CC O14879; Q16543: CDC37; NbExp=4; IntAct=EBI-745127, EBI-295634; CC O14879; Q92905: COPS5; NbExp=4; IntAct=EBI-745127, EBI-594661; CC O14879; Q05D60: DEUP1; NbExp=4; IntAct=EBI-745127, EBI-748597; CC O14879; Q0P5U8: FLJ90650; NbExp=3; IntAct=EBI-745127, EBI-10226698; CC O14879; Q96CS2: HAUS1; NbExp=3; IntAct=EBI-745127, EBI-2514791; CC O14879; P09914: IFIT1; NbExp=12; IntAct=EBI-745127, EBI-745117; CC O14879; Q5T764: IFIT1B; NbExp=6; IntAct=EBI-745127, EBI-3507164; CC O14879; P09913: IFIT2; NbExp=6; IntAct=EBI-745127, EBI-3507167; CC O14879; Q8IZ03: IFIT2; NbExp=4; IntAct=EBI-745127, EBI-746217; CC O14879; O14879: IFIT3; NbExp=3; IntAct=EBI-745127, EBI-745127; CC O14879; Q8IXX5: TMEM183A; NbExp=7; IntAct=EBI-745127, EBI-2841953; CC O14879; P09493-10: TPM1; NbExp=4; IntAct=EBI-745127, EBI-12123928; CC O14879; P06753: TPM3; NbExp=5; IntAct=EBI-745127, EBI-355607; CC O14879; Q5VU62: TPM3; NbExp=3; IntAct=EBI-745127, EBI-10184033; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17050680, CC ECO:0000269|PubMed:18706081}. Mitochondrion CC {ECO:0000269|PubMed:21813773}. CC -!- TISSUE SPECIFICITY: Expression significantly higher in peripheral blood CC mononuclear cells (PBMCs) and monocytes from systemic lupus CC erythematosus (SLE) patients than in those from healthy individuals (at CC protein level). Spleen, lung, leukocytes, lymph nodes, placenta, bone CC marrow and fetal liver. {ECO:0000269|PubMed:18706081}. CC -!- INDUCTION: By type I interferons, dsRNAs and viruses. CC {ECO:0000269|PubMed:17050680}. CC -!- SIMILARITY: Belongs to the IFIT family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF026939; AAB95160.1; -; mRNA. DR EMBL; AF083470; AAC63524.1; -; mRNA. DR EMBL; U52513; AAB40606.1; -; mRNA. DR EMBL; BT007284; AAP35948.1; -; mRNA. DR EMBL; BC001383; AAH01383.1; -; mRNA. DR EMBL; BC004977; AAH04977.1; -; mRNA. DR CCDS; CCDS31241.1; -. DR CCDS; CCDS7402.1; -. DR RefSeq; NP_001026853.1; NM_001031683.3. DR RefSeq; NP_001540.2; NM_001549.5. DR PDB; 6C6K; X-ray; 2.54 A; C/D=416-459. DR PDBsum; 6C6K; -. DR AlphaFoldDB; O14879; -. DR SMR; O14879; -. DR BioGRID; 109660; 81. DR DIP; DIP-37890N; -. DR IntAct; O14879; 66. DR MINT; O14879; -. DR STRING; 9606.ENSP00000360876; -. DR GlyCosmos; O14879; 2 sites, 1 glycan. DR GlyGen; O14879; 2 sites, 1 O-linked glycan (2 sites). DR iPTMnet; O14879; -. DR PhosphoSitePlus; O14879; -. DR BioMuta; IFIT3; -. DR EPD; O14879; -. DR jPOST; O14879; -. DR MassIVE; O14879; -. DR PaxDb; 9606-ENSP00000360883; -. DR PeptideAtlas; O14879; -. DR ProteomicsDB; 48279; -. DR Pumba; O14879; -. DR Antibodypedia; 30262; 308 antibodies from 31 providers. DR CPTC; O14879; 1 antibody. DR DNASU; 3437; -. DR Ensembl; ENST00000371811.4; ENSP00000360876.4; ENSG00000119917.15. DR Ensembl; ENST00000371818.9; ENSP00000360883.4; ENSG00000119917.15. DR Ensembl; ENST00000679781.1; ENSP00000505987.1; ENSG00000119917.15. DR GeneID; 3437; -. DR KEGG; hsa:3437; -. DR MANE-Select; ENST00000371818.9; ENSP00000360883.4; NM_001549.6; NP_001540.2. DR AGR; HGNC:5411; -. DR CTD; 3437; -. DR DisGeNET; 3437; -. DR GeneCards; IFIT3; -. DR HGNC; HGNC:5411; IFIT3. DR HPA; ENSG00000119917; Low tissue specificity. DR MIM; 604650; gene. DR neXtProt; NX_O14879; -. DR OpenTargets; ENSG00000119917; -. DR PharmGKB; PA29651; -. DR VEuPathDB; HostDB:ENSG00000119917; -. DR eggNOG; KOG1124; Eukaryota. DR GeneTree; ENSGT00950000182946; -. DR HOGENOM; CLU_043482_0_0_1; -. DR InParanoid; O14879; -. DR OMA; NPYSIES; -. DR OrthoDB; 5401272at2759; -. DR PhylomeDB; O14879; -. DR TreeFam; TF342671; -. DR PathwayCommons; O14879; -. DR Reactome; R-HSA-909733; Interferon alpha/beta signaling. DR SignaLink; O14879; -. DR BioGRID-ORCS; 3437; 10 hits in 1152 CRISPR screens. DR ChiTaRS; IFIT3; human. DR GeneWiki; IFIT3; -. DR GenomeRNAi; 3437; -. DR Pharos; O14879; Tbio. DR PRO; PR:O14879; -. DR Proteomes; UP000005640; Chromosome 10. DR RNAct; O14879; Protein. DR Bgee; ENSG00000119917; Expressed in trigeminal ganglion and 194 other cell types or tissues. DR ExpressionAtlas; O14879; baseline and differential. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0005829; C:cytosol; IDA:HPA. DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR GO; GO:0140374; P:antiviral innate immune response; IDA:UniProt. DR GO; GO:0051607; P:defense response to virus; IBA:GO_Central. DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB. DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:UniProtKB. DR GO; GO:0009615; P:response to virus; IMP:UniProtKB. DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 3. DR InterPro; IPR011990; TPR-like_helical_dom_sf. DR InterPro; IPR019734; TPR_repeat. DR PANTHER; PTHR10271; INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS; 1. DR PANTHER; PTHR10271:SF3; INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS 3; 1. DR Pfam; PF13424; TPR_12; 1. DR Pfam; PF13176; TPR_7; 1. DR Pfam; PF13181; TPR_8; 2. DR SMART; SM00028; TPR; 5. DR SUPFAM; SSF48452; TPR-like; 2. DR PROSITE; PS50005; TPR; 5. DR PROSITE; PS50293; TPR_REGION; 1. DR Genevisible; O14879; HS. PE 1: Evidence at protein level; KW 3D-structure; Antiviral defense; Cytoplasm; Immunity; Innate immunity; KW Mitochondrion; Phosphoprotein; Reference proteome; Repeat; TPR repeat. FT CHAIN 1..490 FT /note="Interferon-induced protein with tetratricopeptide FT repeats 3" FT /id="PRO_0000106349" FT REPEAT 51..84 FT /note="TPR 1" FT REPEAT 94..127 FT /note="TPR 2" FT REPEAT 136..169 FT /note="TPR 3" FT REPEAT 172..206 FT /note="TPR 4" FT REPEAT 207..240 FT /note="TPR 5" FT REPEAT 241..274 FT /note="TPR 6" FT REPEAT 415..448 FT /note="TPR 7" FT REPEAT 450..481 FT /note="TPR 8" FT REGION 467..490 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 471..490 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 203 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:18669648, FT ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231" FT MOD_RES 237 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:18691976" FT MOD_RES 478 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:18669648, FT ECO:0007744|PubMed:24275569" FT CONFLICT 44 FT /note="F -> S (in Ref. 5; AAH04977)" FT /evidence="ECO:0000305" FT CONFLICT 359 FT /note="Q -> QQ (in Ref. 2; AAB40606)" FT /evidence="ECO:0000305" FT CONFLICT 435 FT /note="Missing (in Ref. 2; AAB40606)" FT /evidence="ECO:0000305" FT HELIX 417..428 FT /evidence="ECO:0007829|PDB:6C6K" FT HELIX 431..445 FT /evidence="ECO:0007829|PDB:6C6K" FT TURN 446..448 FT /evidence="ECO:0007829|PDB:6C6K" FT HELIX 453..457 FT /evidence="ECO:0007829|PDB:6C6K" SQ SEQUENCE 490 AA; 55985 MW; B9F042D4DF7151D2 CRC64; MSEVTKNSLE KILPQLKCHF TWNLFKEDSV SRDLEDRVCN QIEFLNTEFK ATMYNLLAYI KHLDGNNEAA LECLRQAEEL IQQEHADQAE IRSLVTWGNY AWVYYHLGRL SDAQIYVDKV KQTCKKFSNP YSIEYSELDC EEGWTQLKCG RNERAKVCFE KALEEKPNNP EFSSGLAIAM YHLDNHPEKQ FSTDVLKQAI ELSPDNQYVK VLLGLKLQKM NKEAEGEQFV EEALEKSPCQ TDVLRSAAKF YRRKGDLDKA IELFQRVLES TPNNGYLYHQ IGCCYKAKVR QMQNTGESEA SGNKEMIEAL KQYAMDYSNK ALEKGLNPLN AYSDLAEFLE TECYQTPFNK EVPDAEKQQS HQRYCNLQKY NGKSEDTAVQ HGLEGLSISK KSTDKEEIKD QPQNVSENLL PQNAPNYWYL QGLIHKQNGD LLQAAKCYEK ELGRLLRDAP SGIGSIFLSA SELEDGSEEM GQGAVSSSPR ELLSNSEQLN //