Reviewed,
UniProtKB/Swiss-Prot O14874 (BCKD_HUMAN)
Last modified
June 16, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial EC=2.7.11.4 Alternative name(s): Branched-chain alpha-ketoacid dehydrogenase kinase Short name=BCKD-kinase Short name=BCKDHKIN | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 412 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex By similarity. |
| Catalytic activity | ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate. |
| Subunit structure | Monomer By similarity. |
| Subcellular location | |
| Tissue specificity | Ubiquitous. |
| Post-translational modification | Autophosphorylated By similarity. |
| Sequence similarities | Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| BCKDHA | P12694 | 1 | EBI-1046765,EBI-1029053 | |
| CAB39 | Q9Y376 | 1 | EBI-1046765,EBI-306905 | |
| CAB39L | Q9H9S4 | 1 | EBI-1046765,EBI-1047244 | |
| FAM98B | Q52LJ0 | 1 | EBI-1046765,EBI-1043130 | |
| RTCD1 | Q5VSU9 | 1 | EBI-1046765,EBI-1045306 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 30 | 30 | Mitochondrion By similarity | ||||||
| Chain | 31 – 412 | 382 | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial | PRO_0000023452 | |||||
Regions | |||||||||
| Domain | 159 – 404 | 246 | Histidine kinase | ||||||
Amino acid modifications | |||||||||
| Modified residue | 31 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 33 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 35 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 52 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 218 | 1 | V → F in AAB82714. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Chuang J.C., Cox R.P., Chuang D.T. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta. |
| [3] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. |
| [4] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| AF026548 mRNA. Translation: AAB82714.1. BC007363 mRNA. Translation: AAH07363.1. | |
| IPI | IPI00298612. |
| RefSeq | NP_005872.2. |
| UniGene | Hs.513520 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GKZ based on UniProtKB Q00972. |
| SMR | O14874. Positions 68-408. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O14874. 6 interactions. |
PTM databases | |
| PhosphoSite | O14874. |
Proteomic databases | |
| PRIDE | O14874. |
Genome annotation databases | |
| Ensembl | ENSG00000103507. Homo sapiens. [Contig view] |
| GeneID | 10295. |
Organism-specific databases | |
| GeneCards | GC16P031024. |
| HGNC | HGNC:16902. BCKDK. |
| HPA | HPA017995. |
| PharmGKB | PA134899581. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | O14874. |
| HOVERGEN | O14874. |
| OMA | O14874. RIKGFRS. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.4. 247. |
Gene expression databases | |
| ArrayExpress | O14874. |
| Bgee | O14874. |
| CleanEx | HS_BCKDK. |
| GermOnline | ENSG00000103507. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR003594. ATP_bd_ATPase. IPR018955. BCDHK/PDK_mit. IPR004358. Sig_transdc_His_kin-like_C. IPR005467. Sig_transdc_His_kinase_core. [Graphical view] |
| Gene3D | G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. |
| Pfam | PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view] |
| PRINTS | PR00344. BCTRLSENSOR. |
| SMART | SM00387. HATPase_c. 1 hit. [Graphical view] |
| PROSITE | PS50109. HIS_KIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 39014. |
Entry information
| Entry name | BCKD_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O14874 Secondary accession number(s): Q96IN5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


