O14874 (BCKD_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 110.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial EC=2.7.11.4 Alternative name(s): Branched-chain alpha-ketoacid dehydrogenase kinase Short name=BCKD-kinase Short name=BCKDHKIN | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 412 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex By similarity. |
| Catalytic activity | ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate. |
| Subunit structure | Monomer By similarity. |
| Subcellular location | |
| Tissue specificity | Ubiquitous. |
| Post-translational modification | |
| Sequence similarities | Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| STAT3 | P40763 | 2 | EBI-1046765,EBI-518675 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 30 | 30 | Mitochondrion By similarity | ||||||
| Chain | 31 – 412 | 382 | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial | PRO_0000023452 | |||||
Regions | |||||||||
| Domain | 159 – 404 | 246 | Histidine kinase | ||||||
Amino acid modifications | |||||||||
| Modified residue | 31 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 33 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 35 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 52 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 184 | 1 | N6-acetyllysine Ref.7 | ||||||
| Modified residue | 192 | 1 | N6-acetyllysine Ref.7 | ||||||
| Modified residue | 233 | 1 | N6-acetyllysine Ref.7 | ||||||
| Modified residue | 245 | 1 | N6-acetyllysine Ref.7 | ||||||
Experimental info | |||||||||
| Sequence conflict | 218 | 1 | V → F in AAB82714. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Chuang J.C., Cox R.P., Chuang D.T. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta. |
| [5] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [6] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [7] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-184; LYS-192; LYS-233 AND LYS-245, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF026548 mRNA. Translation: AAB82714.1. CR542093 mRNA. Translation: CAG46890.1. CH471192 Genomic DNA. Translation: EAW52161.1. BC007363 mRNA. Translation: AAH07363.1. |
| IPI | IPI00298612. |
| RefSeq | NP_001116429.1. NM_001122957.1. NP_005872.2. NM_005881.2. |
| UniGene | Hs.513520. |
3D structure databases | |
| ProteinModelPortal | O14874. |
| SMR | O14874. Positions 68-408. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O14874. 3 interactions. |
| MINT | MINT-2998419. |
| STRING | O14874. |
PTM databases | |
| PhosphoSite | O14874. |
Proteomic databases | |
| PRIDE | O14874. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000219794; ENSP00000219794; ENSG00000103507. ENST00000394951; ENSP00000378405; ENSG00000103507. |
| GeneID | 10295. |
| KEGG | hsa:10295. |
| UCSC | uc002eaw.2. human. |
Organism-specific databases | |
| CTD | 10295. |
| GeneCards | GC16P031117. |
| H-InvDB | HIX0012980. |
| HGNC | HGNC:16902. BCKDK. |
| HPA | HPA017995. |
| neXtProt | NX_O14874. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG15303. |
| GeneTree | ENSGT00550000074574. |
| HOGENOM | HBG281443. |
| HOVERGEN | HBG004829. |
| InParanoid | O14874. |
| OMA | NQSAIDV. |
| OrthoDB | EOG49ZXPB. |
| PhylomeDB | O14874. |
Gene expression databases | |
| ArrayExpress | O14874. |
| Bgee | O14874. |
| CleanEx | HS_BCKDK. |
| Genevestigator | O14874. |
| GermOnline | ENSG00000103507. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR003594. ATPase-like_ATP-bd. IPR018955. BCDHK/PDK_N. IPR004358. Sig_transdc_His_kin-like_C. IPR005467. Sig_transdc_His_kinase_core. [Graphical view] |
| Gene3D | G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:1.20.140.20. BCDHK/PDK_N. 1 hit. |
| KO | K00905. |
| Pfam | PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view] |
| PRINTS | PR00344. BCTRLSENSOR. |
| SMART | SM00387. HATPase_c. 1 hit. [Graphical view] |
| SUPFAM | SSF55874. ATP_bd_ATPase. 1 hit. SSF69012. BCDHK/PDK_N. 1 hit. |
| PROSITE | PS50109. HIS_KIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 39014. |
Entry information
| Entry name | BCKD_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O14874 Secondary accession number(s): Q6FGL4, Q96IN5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| SIMILARITY comments Index of protein domains and families |

Clusters with