##gff-version 3 O14862 UniProtKB Chain 1 343 . . . ID=PRO_0000153726;Note=Interferon-inducible protein AIM2 O14862 UniProtKB Domain 1 87 . . . Note=Pyrin;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00061 O14862 UniProtKB Domain 138 337 . . . Note=HIN-200;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00106 O14862 UniProtKB Natural variant 32 32 . . . ID=VAR_022022;Note=E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17726700;Dbxref=dbSNP:rs2276405,PMID:17726700 O14862 UniProtKB Natural variant 304 304 . . . ID=VAR_043379;Note=C->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17726700;Dbxref=dbSNP:rs778047649,PMID:17726700 O14862 UniProtKB Mutagenesis 10 11 . . . Note=Impaired double-stranded DNA (dsDNA)-binding%2C preventing homooligomerization. LL->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 11 11 . . . Note=Impaired homooligomerization. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 14 14 . . . Note=Fails to activate interleukin-1 beta production. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19158679;Dbxref=PMID:19158679 O14862 UniProtKB Mutagenesis 19 23 . . . Note=In Mut2%3B abolished interaction with PYCARD/ASC. Abolished ability to bind double-stranded DNA (dsDNA). DEELD->AAALA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:23530044,ECO:0000269|PubMed:26197926;Dbxref=PMID:23530044,PMID:26197926 O14862 UniProtKB Mutagenesis 21 21 . . . Note=Impaired ability to nucleate with PYCARD/ASC. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 23 23 . . . Note=Impaired homooligomerization. D->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 27 28 . . . Note=In Mut1%3B abolished interaction with PYCARD/ASC. FF->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:23530044;Dbxref=PMID:23530044 O14862 UniProtKB Mutagenesis 27 27 . . . Note=Abolished ability to homooligomerize. F->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24406744;Dbxref=PMID:24406744 O14862 UniProtKB Mutagenesis 27 27 . . . Note=Strongly impaired ability to homooligomerize. F->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24406744;Dbxref=PMID:24406744 O14862 UniProtKB Mutagenesis 27 27 . . . Note=Impaired ability to homooligomerize. F->W%2CY;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24406744;Dbxref=PMID:24406744 O14862 UniProtKB Mutagenesis 36 36 . . . Note=Impaired homooligomerization. A->R%2CD;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 46 46 . . . Note=Impaired homooligomerization. I->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 50 50 . . . Note=Impaired double-stranded DNA (dsDNA)-binding%2C preventing homooligomerization. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 73 73 . . . Note=Impaired ability to form AIM2 inflammasome filaments in response to double-stranded DNA (dsDNA). N->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 75 75 . . . Note=Impaired ability to nucleate with PYCARD/ASC. M->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33980849;Dbxref=PMID:33980849 O14862 UniProtKB Mutagenesis 147 147 . . . Note=Strongly reduced ability to homooligomerize upon double-stranded DNA (dsDNA)-binding. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 160 160 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-K162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 162 162 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 163 163 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 165 165 . . . Note=Impairs DNA binding. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19158679;Dbxref=PMID:19158679 O14862 UniProtKB Mutagenesis 167 167 . . . Note=Strongly reduced ability to homooligomerize upon double-stranded DNA (dsDNA)-binding. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 173 173 . . . Note=Impaired double-stranded DNA (dsDNA)-binding%2C preventing homooligomerization. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 198 198 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 204 204 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 244 244 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 251 251 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 258 258 . . . Note=Impaired double-stranded DNA (dsDNA)-binding%2C preventing homooligomerization. Q->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 272 272 . . . Note=Strongly reduced ability to homooligomerize upon double-stranded DNA (dsDNA)-binding. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26197926;Dbxref=PMID:26197926 O14862 UniProtKB Mutagenesis 309 309 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 311 311 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 335 335 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Mutagenesis 337 337 . . . Note=Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204. Impairs DNA binding%3B when associated with A-160%3B A-162%3B A-163%3B A-198%3B A-204%3B A-244%3B A-251%3B A-309%3B A-311%3B A-355 and A-337. I->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22483801;Dbxref=PMID:22483801 O14862 UniProtKB Sequence conflict 253 253 . . . Note=N->D;Ontology_term=ECO:0000305;evidence=ECO:0000305 O14862 UniProtKB Helix 1 11 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Helix 14 16 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Helix 19 29 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Turn 30 32 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Helix 37 40 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Helix 45 56 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Helix 58 71 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Helix 75 91 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4O7Q O14862 UniProtKB Beta strand 148 150 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN2 O14862 UniProtKB Beta strand 154 161 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 165 169 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 172 182 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 187 192 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Helix 195 199 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 206 214 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 219 221 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 225 229 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Helix 240 247 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Helix 252 256 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 263 275 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 277 286 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 289 296 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Helix 300 302 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 309 319 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 321 327 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5 O14862 UniProtKB Beta strand 333 337 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RN5