O14843 (FFAR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Free fatty acid receptor 3

Alternative name(s):
G-protein coupled receptor 41

Gene names

Name:	FFAR3
Synonyms:	GPR41

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate > acetate > formate. Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Highest level in adipose tissue, and lower expression across all tissues tested. Ref.4
Polymorphism	Sequence differences between FFAR3 and GPR42 are due to polymorphisms. The consensus sequences within the coding regions are identical for the two genes.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Lipid-binding
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Free fatty acid receptor 3		PRO_0000069569
Regions
	Topological domain	1 – 19	19	Extracellular Potential
	Transmembrane	20 – 40	21	Helical; Name=1; Potential
	Topological domain	41 – 47	7	Cytoplasmic
	Transmembrane	48 – 68	21	Helical; Name=2; Potential
	Topological domain	69 – 90	22	Extracellular Potential
	Transmembrane	91 – 111	21	Helical; Name=3; Potential
	Topological domain	112 – 132	21	Cytoplasmic Potential
	Transmembrane	133 – 153	21	Helical; Name=4; Potential
	Topological domain	154 – 178	25	Extracellular Potential
	Transmembrane	179 – 199	21	Helical; Name=5; Potential
	Topological domain	200 – 222	23	Cytoplasmic Potential
	Transmembrane	223 – 243	21	Helical; Name=6; Potential
	Topological domain	244 – 258	15	Extracellular Potential
	Transmembrane	259 – 279	21	Helical; Name=7; Potential
	Topological domain	280 – 346	67	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	44	1	Q → R. Ref.6		VAR_062854
	Natural variant	45	1	R → C. Ref.6 Corresponds to variant rs423385 [ dbSNP | Ensembl ].		VAR_062855
	Natural variant	174	1	R → W Abolishes activation by propionate. Ref.4 Ref.6		VAR_062856
	Natural variant	227	1	L → V. Ref.6 Corresponds to variant rs403989 [ dbSNP | Ensembl ].		VAR_062857
	Natural variant	256	1	A → V. Ref.6 Corresponds to variant rs424715 [ dbSNP | Ensembl ].		VAR_062858
	Natural variant	346	1	S → N. Ref.6		VAR_062962

Sequences

	Sequence		Length	Mass (Da)
	O14843 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B3B19D62D11B6BA1	FASTA	346	38,649
	10 20 30 40 50 60 MDTGPDQSYF SGNHWFVFSV YLLTFLVGLP LNLLALVVFV GKLQRRPVAV DVLLLNLTAS 70 80 90 100 110 120 DLLLLLFLPF RMVEAANGMH WPLPFILCPL SGFIFFTTIY LTALFLAAVS IERFLSVAHP 130 140 150 160 170 180 LWYKTRPRLG QAGLVSVACW LLASAHCSVV YVIEFSGDIS HSQGTNGTCY LEFRKDQLAI 190 200 210 220 230 240 LLPVRLEMAV VLFVVPLIIT SYCYSRLVWI LGRGGSHRRQ RRVAGLLAAT LLNFLVCFGP 250 260 270 280 290 300 YNVSHVVGYI CGESPAWRIY VTLLSTLNSC VDPFVYYFSS SGFQADFHEL LRRLCGLWGQ 310 320 330 340 WQQESSMELK EQKGGEEQRA DRPAERKTSE HSQGCGTGGQ VACAES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1." Sawzdargo M., George S.R., Nguyen T., Xu S., Kolakowski L.F. Jr., O'Dowd B.F. Biochem. Biophys. Res. Commun. 239:543-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary.
[4]	"The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids." Brown A.J., Goldsworthy S.M., Barnes A.A., Eilert M.M., Tcheang L., Daniels D., Muir A.I., Wigglesworth M.J., Kinghorn I., Fraser N.J., Pike N.B., Strum J.C., Steplewski K.M., Murdock P.R., Holder J.C., Marshall F.H., Szekeres P.G., Wilson S. , Ignar D.M., Foord S.M., Wise A., Dowell S.J. J. Biol. Chem. 278:11312-11319(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CHARACTERIZATION OF VARIANT TRP-174, TISSUE SPECIFICITY.
[5]	"Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation." Le Poul E., Loison C., Struyf S., Springael J.Y., Lannoy V., Decobecq M.E., Brezillon S., Dupriez V., Vassart G., Van Damme J., Parmentier M., Detheux M. J. Biol. Chem. 278:25481-25489(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CHARACTERIZATION.
[6]	"Sequence polymorphisms provide a common consensus sequence for GPR41 and GPR42." Liaw C.W., Connolly D.T. DNA Cell Biol. 28:555-560(2009) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM, VARIANTS ARG-44; CYS-45; TRP-174; VAL-227; VAL-256 AND ASN-346.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF024688 Genomic DNA. Translation: AAB86711.1. U62631 Genomic DNA. No translation available. BC035657 mRNA. Translation: AAH35657.1. BC113695 mRNA. Translation: AAI13696.1. BC148269 mRNA. Translation: AAI48270.1.
IPI	IPI00024208.
PIR	JC5715.
RefSeq	NP_005295.1. NM_005304.3.
UniGene	Hs.248055.
3D structure databases
ProteinModelPortal	O14843.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000246538.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PaxDb	O14843.
PRIDE	O14843.
Protocols and materials databases
DNASU	2865.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327809; ENSP00000328230; ENSG00000185897. ENST00000594310; ENSP00000469522; ENSG00000185897.
GeneID	2865.
KEGG	hsa:2865.
UCSC	uc002nzd.3. human.
Organism-specific databases
CTD	2865.
GeneCards	GC19P035850.
HGNC	HGNC:4499. FFAR3.
HPA	HPA044681.
MIM	603821. gene.
neXtProt	NX_O14843.
PharmGKB	PA28888.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47521.
HOGENOM	HOG000026778.
HOVERGEN	HBG051811.
InParanoid	O14843.
KO	K04326.
OMA	LEMAVIL.
OrthoDB	EOG4XSKQX.
PhylomeDB	O14843.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	O14843.
CleanEx	HS_FFAR3.
Genevestigator	O14843.
GermOnline	ENSG00000185897. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR013312. GPR40-rel_orph. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01904. GPR40FAMILY.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5201.
GenomeRNAi	2865.
NextBio	11297.
SOURCE	Search...

Entry information

Entry name

FFAR3_HUMAN

Accession

Primary (citable) accession number: O14843
Secondary accession number(s): B2RWM8, Q14CM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.