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O14843 (FFAR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Free fatty acid receptor 3
Alternative name(s):
G-protein coupled receptor 41
Gene names
Name:FFAR3
Synonyms:GPR41
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate > acetate > formate. Ref.4 Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Highest level in adipose tissue, and lower expression across all tissues tested. Ref.4

Polymorphism

Sequence differences between FFAR3 and GPR42 are due to polymorphisms. The consensus sequences within the coding regions are identical for the two genes.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Free fatty acid receptor 3
PRO_0000069569

Regions

Topological domain1 – 1919Extracellular Potential
Transmembrane20 – 4021Helical; Name=1; Potential
Topological domain41 – 477Cytoplasmic
Transmembrane48 – 6821Helical; Name=2; Potential
Topological domain69 – 9022Extracellular Potential
Transmembrane91 – 11121Helical; Name=3; Potential
Topological domain112 – 13221Cytoplasmic Potential
Transmembrane133 – 15321Helical; Name=4; Potential
Topological domain154 – 17825Extracellular Potential
Transmembrane179 – 19921Helical; Name=5; Potential
Topological domain200 – 22223Cytoplasmic Potential
Transmembrane223 – 24321Helical; Name=6; Potential
Topological domain244 – 25815Extracellular Potential
Transmembrane259 – 27921Helical; Name=7; Potential
Topological domain280 – 34667Cytoplasmic Potential

Amino acid modifications

Glycosylation1661N-linked (GlcNAc...) Potential

Natural variations

Natural variant441Q → R. Ref.6
VAR_062854
Natural variant451R → C. Ref.6
Corresponds to variant rs423385 [ dbSNP | Ensembl ].
VAR_062855
Natural variant1741R → W Abolishes activation by propionate. Ref.4 Ref.6
VAR_062856
Natural variant2271L → V. Ref.6
Corresponds to variant rs403989 [ dbSNP | Ensembl ].
VAR_062857
Natural variant2561A → V. Ref.6
Corresponds to variant rs424715 [ dbSNP | Ensembl ].
VAR_062858
Natural variant3461S → N. Ref.6
VAR_062962

Sequences

Sequence LengthMass (Da)Tools
O14843 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B3B19D62D11B6BA1

FASTA34638,649
        10         20         30         40         50         60 
MDTGPDQSYF SGNHWFVFSV YLLTFLVGLP LNLLALVVFV GKLQRRPVAV DVLLLNLTAS 

        70         80         90        100        110        120 
DLLLLLFLPF RMVEAANGMH WPLPFILCPL SGFIFFTTIY LTALFLAAVS IERFLSVAHP 

       130        140        150        160        170        180 
LWYKTRPRLG QAGLVSVACW LLASAHCSVV YVIEFSGDIS HSQGTNGTCY LEFRKDQLAI 

       190        200        210        220        230        240 
LLPVRLEMAV VLFVVPLIIT SYCYSRLVWI LGRGGSHRRQ RRVAGLLAAT LLNFLVCFGP 

       250        260        270        280        290        300 
YNVSHVVGYI CGESPAWRIY VTLLSTLNSC VDPFVYYFSS SGFQADFHEL LRRLCGLWGQ 

       310        320        330        340 
WQQESSMELK EQKGGEEQRA DRPAERKTSE HSQGCGTGGQ VACAES 

« Hide

References

« Hide 'large scale' references
[1]"A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1."
Sawzdargo M., George S.R., Nguyen T., Xu S., Kolakowski L.F. Jr., O'Dowd B.F.
Biochem. Biophys. Res. Commun. 239:543-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Ovary.
[4]"The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids."
Brown A.J., Goldsworthy S.M., Barnes A.A., Eilert M.M., Tcheang L., Daniels D., Muir A.I., Wigglesworth M.J., Kinghorn I., Fraser N.J., Pike N.B., Strum J.C., Steplewski K.M., Murdock P.R., Holder J.C., Marshall F.H., Szekeres P.G., Wilson S. expand/collapse author list , Ignar D.M., Foord S.M., Wise A., Dowell S.J.
J. Biol. Chem. 278:11312-11319(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION OF VARIANT TRP-174, TISSUE SPECIFICITY.
[5]"Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation."
Le Poul E., Loison C., Struyf S., Springael J.Y., Lannoy V., Decobecq M.E., Brezillon S., Dupriez V., Vassart G., Van Damme J., Parmentier M., Detheux M.
J. Biol. Chem. 278:25481-25489(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION.
[6]"Sequence polymorphisms provide a common consensus sequence for GPR41 and GPR42."
Liaw C.W., Connolly D.T.
DNA Cell Biol. 28:555-560(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: POLYMORPHISM, VARIANTS ARG-44; CYS-45; TRP-174; VAL-227; VAL-256 AND ASN-346.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF024688 Genomic DNA. Translation: AAB86711.1.
U62631 Genomic DNA. No translation available.
BC035657 mRNA. Translation: AAH35657.1.
BC113695 mRNA. Translation: AAI13696.1.
BC148269 mRNA. Translation: AAI48270.1.
PIRJC5715.
RefSeqNP_005295.1. NM_005304.3.
UniGeneHs.248055.

3D structure databases

ProteinModelPortalO14843.
SMRO14843. Positions 6-290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000246538.

Chemistry

ChEMBLCHEMBL5201.
GuidetoPHARMACOLOGY227.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbO14843.
PRIDEO14843.

Protocols and materials databases

DNASU2865.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000327809; ENSP00000328230; ENSG00000185897.
ENST00000594310; ENSP00000469522; ENSG00000185897.
GeneID2865.
KEGGhsa:2865.
UCSCuc002nzd.3. human.

Organism-specific databases

CTD2865.
GeneCardsGC19P035851.
HGNCHGNC:4499. FFAR3.
HPAHPA044681.
MIM603821. gene.
neXtProtNX_O14843.
PharmGKBPA28888.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47521.
HOGENOMHOG000026778.
HOVERGENHBG051811.
InParanoidO14843.
KOK04326.
OMAYVMEFSG.
OrthoDBEOG7SFHXH.
PhylomeDBO14843.
TreeFamTF350010.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeO14843.
CleanExHS_FFAR3.
GenevestigatorO14843.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiFree_fatty_acid_receptor_3.
GenomeRNAi2865.
NextBio11297.
PROO14843.
SOURCESearch...

Entry information

Entry nameFFAR3_HUMAN
AccessionPrimary (citable) accession number: O14843
Secondary accession number(s): B2RWM8, Q14CM7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries