O14842 (FFAR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Free fatty acid receptor 1

Alternative name(s):
G-protein coupled receptor 40

Gene names

Name:	FFAR1
Synonyms:	GPR40

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed abundantly in pancreatic beta cells. Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Lipid-binding
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	energy reserve metabolic process Traceable author statement. Source: Reactome
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	G-protein coupled receptor activity Traceable author statement. Source: ProtInc guanyl-nucleotide exchange factor activity Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Free fatty acid receptor 1		PRO_0000069567
Regions
	Topological domain	1 – 10	10	Extracellular Potential
	Transmembrane	11 – 31	21	Helical; Name=1; Potential
	Topological domain	32 – 42	11	Cytoplasmic
	Transmembrane	43 – 63	21	Helical; Name=2; Potential
	Topological domain	64 – 81	18	Extracellular Potential
	Transmembrane	82 – 102	21	Helical; Name=3; Potential
	Topological domain	103 – 123	21	Cytoplasmic Potential
	Transmembrane	124 – 144	21	Helical; Name=4; Potential
	Topological domain	145 – 178	34	Extracellular Potential
	Transmembrane	179 – 197	19	Helical; Name=5; Potential
	Topological domain	198 – 221	24	Cytoplasmic Potential
	Transmembrane	222 – 242	21	Helical; Name=6; Potential
	Topological domain	243 – 258	16	Extracellular Potential
	Transmembrane	259 – 279	21	Helical; Name=7; Potential
	Topological domain	280 – 300	21	Cytoplasmic Potential
Amino acid modifications
	Modified residue	44	1	Phosphotyrosine By similarity
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	211	1	R → H. Ref.2 Corresponds to variant rs2301151 [ dbSNP | Ensembl ].		VAR_020076
Experimental info
	Sequence conflict	24	1	V → A in AAH95536. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O14842 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 77EF27DACD93E80B	FASTA	300	31,457
	10 20 30 40 50 60 MDLPPQLSFG LYVAAFALGF PLNVLAIRGA TAHARLRLTP SLVYALNLGC SDLLLTVSLP 70 80 90 100 110 120 LKAVEALASG AWPLPASLCP VFAVAHFFPL YAGGGFLAAL SAGRYLGAAF PLGYQAFRRP 130 140 150 160 170 180 CYSWGVCAAI WALVLCHLGL VFGLEAPGGW LDHSNTSLGI NTPVNGSPVC LEAWDPASAG 190 200 210 220 230 240 PARFSLSLLL FFLPLAITAF CYVGCLRALA RSGLTHRRKL RAAWVAGGAL LTLLLCVGPY 250 260 270 280 290 300 NASNVASFLY PNLGGSWRKL GLITGAWSVV LNPLVTGYLG RGPGLKTVCA ARTQGGKSQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1." Sawzdargo M., George S.R., Nguyen T., Xu S., Kolakowski L.F. Jr., O'Dowd B.F. Biochem. Biophys. Res. Commun. 239:543-547(1997) [PubMed: 9344866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-211.
[3]	"The orphan G protein-coupled receptor GPR40 is activated by medium and long-chain fatty acids." Briscoe C.P., Tadayyon M., Andrews J.L., Benson W.G., Chambers J.K., Eilert M.M., Ellis C., Elshourbagy N.A., Goetz A.S., Minnick D.T., Murdock P.R., Sauls H.R. Jr., Shabon U., Spinage L.D., Strum J.C., Szekeres P.G., Tan K.B., Way J.M. , Ignar D.M., Wilson S., Muir A.I. J. Biol. Chem. 278:11303-11311(2003) [PubMed: 12496284] [Abstract] Cited for: FUNCTION.
[4]	"GPR40 gene expression in human pancreas and insulinoma." Tomita T., Masuzaki H., Noguchi M., Iwakura H., Fujikura J., Tanaka T., Ebihara K., Kawamura J., Komoto I., Kawaguchi Y., Fujimoto K., Doi R., Shimada Y., Hosoda K., Imamura M., Nakao K. Biochem. Biophys. Res. Commun. 338:1788-1790(2005) [PubMed: 16289108] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF024687 Genomic DNA. Translation: AAB86710.1. BC095536 mRNA. Translation: AAH95536.1. BC120944 mRNA. Translation: AAI20945.1.
IPI	IPI00024206.
PIR	JC5714.
RefSeq	NP_005294.1. NM_005303.2.
UniGene	Hs.248127.
3D structure databases
ProteinModelPortal	O14842.
SMR	O14842. Positions 91-122.
ModBase	Search...
Protein-protein interaction databases
STRING	O14842.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O14842.
Proteomic databases
PRIDE	O14842.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000246553; ENSP00000246553; ENSG00000126266.
GeneID	2864.
KEGG	hsa:2864.
UCSC	uc002nzc.1. human.
Organism-specific databases
CTD	2864.
GeneCards	GC19P035842.
H-InvDB	HIX0202910.
HGNC	HGNC:4498. FFAR1.
MIM	603820. gene.
neXtProt	NX_O14842.
PharmGKB	PA28887.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07044.
HOGENOM	HBG444550.
HOVERGEN	HBG080696.
InParanoid	O14842.
OMA	CLEAWDP.
OrthoDB	EOG470THV.
PhylomeDB	O14842.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O14842.
Bgee	O14842.
CleanEx	HS_FFAR1.
Genevestigator	O14842.
GermOnline	ENSG00000126266. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR013312. GPR40-rel_recept. IPR013313. GPR40_recept_FA. [Graphical view]
KO	K04325.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01905. FATTYACIDR. PR00237. GPCRRHODOPSN. PR01904. GPR40FAMILY.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00159. Icosapent.
NextBio	11293.
SOURCE	Search...

Entry information

Entry name

FFAR1_HUMAN

Accession

Primary (citable) accession number: O14842
Secondary accession number(s): Q0VAS2, Q4VBL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.