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O14842 (FFAR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Free fatty acid receptor 1
Alternative name(s):
G-protein coupled receptor 40
Gene names
Name:FFAR1
Synonyms:GPR40
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Ref.3

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed abundantly in pancreatic beta cells. Ref.4

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Free fatty acid receptor 1
PRO_0000069567

Regions

Topological domain1 – 1010Extracellular Potential
Transmembrane11 – 3121Helical; Name=1; Potential
Topological domain32 – 4211Cytoplasmic
Transmembrane43 – 6321Helical; Name=2; Potential
Topological domain64 – 8118Extracellular Potential
Transmembrane82 – 10221Helical; Name=3; Potential
Topological domain103 – 12321Cytoplasmic Potential
Transmembrane124 – 14421Helical; Name=4; Potential
Topological domain145 – 17834Extracellular Potential
Transmembrane179 – 19719Helical; Name=5; Potential
Topological domain198 – 22124Cytoplasmic Potential
Transmembrane222 – 24221Helical; Name=6; Potential
Topological domain243 – 25816Extracellular Potential
Transmembrane259 – 27921Helical; Name=7; Potential
Topological domain280 – 30021Cytoplasmic Potential

Amino acid modifications

Glycosylation1551N-linked (GlcNAc...) Potential

Natural variations

Natural variant2111R → H. Ref.2
Corresponds to variant rs2301151 [ dbSNP | Ensembl ].
VAR_020076

Experimental info

Sequence conflict241V → A in AAH95536. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O14842 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 77EF27DACD93E80B

FASTA30031,457
        10         20         30         40         50         60 
MDLPPQLSFG LYVAAFALGF PLNVLAIRGA TAHARLRLTP SLVYALNLGC SDLLLTVSLP 

        70         80         90        100        110        120 
LKAVEALASG AWPLPASLCP VFAVAHFFPL YAGGGFLAAL SAGRYLGAAF PLGYQAFRRP 

       130        140        150        160        170        180 
CYSWGVCAAI WALVLCHLGL VFGLEAPGGW LDHSNTSLGI NTPVNGSPVC LEAWDPASAG 

       190        200        210        220        230        240 
PARFSLSLLL FFLPLAITAF CYVGCLRALA RSGLTHRRKL RAAWVAGGAL LTLLLCVGPY 

       250        260        270        280        290        300 
NASNVASFLY PNLGGSWRKL GLITGAWSVV LNPLVTGYLG RGPGLKTVCA ARTQGGKSQK 

« Hide

References

« Hide 'large scale' references
[1]"A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1."
Sawzdargo M., George S.R., Nguyen T., Xu S., Kolakowski L.F. Jr., O'Dowd B.F.
Biochem. Biophys. Res. Commun. 239:543-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-211.
[3]"The orphan G protein-coupled receptor GPR40 is activated by medium and long-chain fatty acids."
Briscoe C.P., Tadayyon M., Andrews J.L., Benson W.G., Chambers J.K., Eilert M.M., Ellis C., Elshourbagy N.A., Goetz A.S., Minnick D.T., Murdock P.R., Sauls H.R. Jr., Shabon U., Spinage L.D., Strum J.C., Szekeres P.G., Tan K.B., Way J.M. expand/collapse author list , Ignar D.M., Wilson S., Muir A.I.
J. Biol. Chem. 278:11303-11311(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"GPR40 gene expression in human pancreas and insulinoma."
Tomita T., Masuzaki H., Noguchi M., Iwakura H., Fujikura J., Tanaka T., Ebihara K., Kawamura J., Komoto I., Kawaguchi Y., Fujimoto K., Doi R., Shimada Y., Hosoda K., Imamura M., Nakao K.
Biochem. Biophys. Res. Commun. 338:1788-1790(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF024687 Genomic DNA. Translation: AAB86710.1.
BC095536 mRNA. Translation: AAH95536.1.
BC120944 mRNA. Translation: AAI20945.1.
CCDSCCDS12458.1.
PIRJC5714.
RefSeqNP_005294.1. NM_005303.2.
UniGeneHs.248127.

3D structure databases

ProteinModelPortalO14842.
SMRO14842. Positions 13-241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000246553.

Chemistry

BindingDBO14842.
ChEMBLCHEMBL4422.
DrugBankDB00159. Icosapent.
GuidetoPHARMACOLOGY225.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteO14842.

Proteomic databases

PaxDbO14842.
PRIDEO14842.

Protocols and materials databases

DNASU2864.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000246553; ENSP00000246553; ENSG00000126266.
GeneID2864.
KEGGhsa:2864.
UCSCuc002nzc.2. human.

Organism-specific databases

CTD2864.
GeneCardsGC19P035842.
HGNCHGNC:4498. FFAR1.
MIM603820. gene.
neXtProtNX_O14842.
PharmGKBPA28887.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG150822.
HOGENOMHOG000236290.
HOVERGENHBG080696.
InParanoidO14842.
KOK04325.
OMALHLGCSD.
OrthoDBEOG7GQXWF.
PhylomeDBO14842.
TreeFamTF350010.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.

Gene expression databases

BgeeO14842.
CleanExHS_FFAR1.
GenevestigatorO14842.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
IPR013313. GPR40_recept_FA.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01905. FATTYACIDR.
PR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiFree_fatty_acid_receptor_1.
GenomeRNAi2864.
NextBio11293.
PROO14842.
SOURCESearch...

Entry information

Entry nameFFAR1_HUMAN
AccessionPrimary (citable) accession number: O14842
Secondary accession number(s): Q0VAS2, Q4VBL4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries