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O14841

- OPLA_HUMAN

UniProt

O14841 - OPLA_HUMAN

Protein

5-oxoprolinase

Gene

OPLAH

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 3 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

    Catalytic activityi

    ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

    GO - Molecular functioni

    1. 5-oxoprolinase (ATP-hydrolyzing) activity Source: Reactome
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutathione biosynthetic process Source: Reactome
    2. glutathione derivative biosynthetic process Source: Reactome
    3. small molecule metabolic process Source: Reactome
    4. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_6960. Glutathione synthesis and recycling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-oxoprolinase (EC:3.5.2.9)
    Alternative name(s):
    5-oxo-L-prolinase
    Short name:
    5-OPase
    Pyroglutamase
    Gene namesi
    Name:OPLAH
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:8149. OPLAH.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Pathology & Biotechi

    Involvement in diseasei

    5-oxoprolinase deficiency (OPLAHD) [MIM:260005]: A disorder characterized by calcium oxalate/carbonate urolithiasis, and excessive urinary 5-oxo-L-proline. Affected individuals have recurrent episodes of vomiting, diarrhea, and abdominal pain.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.

    Organism-specific databases

    MIMi260005. phenotype.
    Orphaneti33572. 5-oxoprolinase deficiency.
    PharmGKBiPA31935.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 128812885-oxoprolinasePRO_0000208577Add
    BLAST

    Proteomic databases

    MaxQBiO14841.
    PaxDbiO14841.
    PRIDEiO14841.

    PTM databases

    PhosphoSiteiO14841.

    Expressioni

    Gene expression databases

    CleanExiHS_OPLAH.
    GenevestigatoriO14841.

    Organism-specific databases

    HPAiHPA026562.
    HPA028260.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi117922. 3 interactions.
    IntActiO14841. 1 interaction.
    STRINGi9606.ENSP00000412071.

    Structurei

    3D structure databases

    ProteinModelPortaliO14841.
    SMRiO14841. Positions 450-525.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the oxoprolinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0146.
    HOGENOMiHOG000047200.
    HOVERGENiHBG007870.
    InParanoidiO14841.
    KOiK01469.
    OMAiVEAYMGH.
    PhylomeDBiO14841.

    Family and domain databases

    InterProiIPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view]
    PfamiPF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O14841-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG     50
    IRRILEQEAG MLLPRDQPLD SSHIASIRMG TTVATNALLE RKGERVALLV 100
    TRGFRDLLHI GTQARGDLFD LAVPMPEVLY EEVLEVDERV VLHRGEAGTG 150
    TPVKGRTGDL LEVQQPVDLG ALRGKLEGLL SRGIRSLAVV LMHSYTWAQH 200
    EQQVGVLARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY LTPAIQRYVQ 250
    GFCRGFQGQL KDVQVLFMRS DGGLAPMDTF SGSSAVLSGP AGGVVGYSAT 300
    TYQQEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN 350
    TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR 400
    LLPASFPCIF GPGENQPLSP EASRKALEAV ATEVNSFLTN GPCPASPLSL 450
    EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA 500
    RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLLYA PETFVQLDQR 550
    LSRLEEQCVD ALQAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA 600
    RSPRAGDFGA AFVERYMREF GFVIPERPVV VDDVRVRGTG RSGLRLEDAP 650
    KAQTGPPRVD KMTQCYFEGG YQETPVYLLA ELGYGHKLHG PCLIIDSNST 700
    ILVEPGCQAE VTKTGDICIS VGAEVPGTVG PQLDPIQLSI FSHRFMSIAE 750
    QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV 800
    QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQTRPVFYV 850
    ASRGHHADIG GITPGSMPPH STMLQQEGAV FLSFKLVQGG VFQEEAVTEA 900
    LRAPGKVPNC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ 950
    AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSSEDHM DDGSPIRLRV 1000
    QISLSQGSAV FDFSGTGPEV FGNLNAPRAV TLSALIYCLR CLVGRDIPLN 1050
    QGCLAPVRVV IPRGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACAAS 1100
    QGCMNNVTLG NAHMGYYETV AGGAGAGPSW HGRSGVHSHM TNTRITDPEI 1150
    LESRYPVILR RFELRRGSGG RGRFRGGDGV TRELLFREEA LLSVLTERRA 1200
    FRPYGLHGGE PGARGLNLLI RKNGRTVNLG GKTSVTVYPG DVFCLHTPGG 1250
    GGYGDPEDPA PPPGSPPQAL AFPEHGSVYE YRRAQEAV 1288
    Length:1,288
    Mass (Da):137,457
    Last modified:February 15, 2005 - v3
    Checksum:i999AC0FA497750F2
    GO

    Sequence cautioni

    The sequence AAB81519.1 differs from that shown. Reason: Frameshift at positions 1046, 1068 and 1080.
    The sequence CAB46426.2 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1084 – 10896QRVVDV → NAWWMF in AAB81519. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti284 – 2841S → R.
    Corresponds to variant rs3935209 [ dbSNP | Ensembl ].
    VAR_050425

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB122018 mRNA. Translation: BAD13434.1.
    BC142672 mRNA. Translation: AAI42673.1.
    AL096750 mRNA. Translation: CAB46426.2. Frameshift.
    AH005594 Genomic DNA. Translation: AAB81519.1. Frameshift.
    PIRiT12537.
    RefSeqiNP_060040.1. NM_017570.4.
    UniGeneiHs.305882.

    Genome annotation databases

    EnsembliENST00000426825; ENSP00000475943; ENSG00000178814.
    GeneIDi26873.
    KEGGihsa:26873.
    UCSCiuc003zar.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB122018 mRNA. Translation: BAD13434.1 .
    BC142672 mRNA. Translation: AAI42673.1 .
    AL096750 mRNA. Translation: CAB46426.2 . Frameshift.
    AH005594 Genomic DNA. Translation: AAB81519.1 . Frameshift.
    PIRi T12537.
    RefSeqi NP_060040.1. NM_017570.4.
    UniGenei Hs.305882.

    3D structure databases

    ProteinModelPortali O14841.
    SMRi O14841. Positions 450-525.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117922. 3 interactions.
    IntActi O14841. 1 interaction.
    STRINGi 9606.ENSP00000412071.

    Chemistry

    DrugBanki DB00142. L-Glutamic Acid.

    PTM databases

    PhosphoSitei O14841.

    Proteomic databases

    MaxQBi O14841.
    PaxDbi O14841.
    PRIDEi O14841.

    Protocols and materials databases

    DNASUi 26873.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000426825 ; ENSP00000475943 ; ENSG00000178814 .
    GeneIDi 26873.
    KEGGi hsa:26873.
    UCSCi uc003zar.3. human.

    Organism-specific databases

    CTDi 26873.
    GeneCardsi GC08M145106.
    HGNCi HGNC:8149. OPLAH.
    HPAi HPA026562.
    HPA028260.
    MIMi 260005. phenotype.
    614243. gene.
    neXtProti NX_O14841.
    Orphaneti 33572. 5-oxoprolinase deficiency.
    PharmGKBi PA31935.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0146.
    HOGENOMi HOG000047200.
    HOVERGENi HBG007870.
    InParanoidi O14841.
    KOi K01469.
    OMAi VEAYMGH.
    PhylomeDBi O14841.

    Enzyme and pathway databases

    Reactomei REACT_6960. Glutathione synthesis and recycling.

    Miscellaneous databases

    GenomeRNAii 26873.
    NextBioi 49396.
    PROi O14841.
    SOURCEi Search...

    Gene expression databases

    CleanExi HS_OPLAH.
    Genevestigatori O14841.

    Family and domain databases

    InterProi IPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view ]
    Pfami PF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery."
      Watanabe T., Abe K., Ishikawa H., Iijima Y.
      Biol. Pharm. Bull. 27:288-294(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-1288.
      Tissue: Testis.
    4. "Human 5-oxo-L-prolinase partial sequence."
      Debella L.R., Wood S.
      Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 901-1096.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "5-Oxoprolinase deficiency: report of the first human OPLAH mutation."
      Almaghlouth I., Mohamed J., Al-Amoudi M., Al-Ahaidib L., Al-Odaib A., Alkuraya F.
      Clin. Genet. 82:193-196(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INVOLVEMENT IN OPLAHD.

    Entry informationi

    Entry nameiOPLA_HUMAN
    AccessioniPrimary (citable) accession number: O14841
    Secondary accession number(s): A5PKY8, Q75W65, Q9Y4Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3