O14841 (OPLA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-oxoprolinase
EC=3.5.2.9

Alternative name(s):
5-oxo-L-prolinase
Short name=5-OPase
Pyroglutamase

Gene names

Name:

OPLAH

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.
Catalytic activity	ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the oxoprolinase family.
Sequence caution	The sequence AAB81519.1 differs from that shown. Reason: Frameshift at positions 1046, 1068 and 1080. The sequence CAB46426.2 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Traceable author statement. Source: Reactome xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	5-oxoprolinase (ATP-hydrolyzing) activity Traceable author statement. Source: Reactome ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1288	1288	5-oxoprolinase		PRO_0000208577
Natural variations
	Natural variant	284	1	S → R. Corresponds to variant rs3935209 [ dbSNP | Ensembl ].		VAR_050425
Experimental info
	Sequence conflict	1084 – 1089	6	QRVVDV → NAWWMF in AAB81519. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O14841 [UniParc]. Last modified February 15, 2005. Version 3. Checksum: 999AC0FA497750F2	FASTA	1,288	137,457
	10 20 30 40 50 60 MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG IRRILEQEAG 70 80 90 100 110 120 MLLPRDQPLD SSHIASIRMG TTVATNALLE RKGERVALLV TRGFRDLLHI GTQARGDLFD 130 140 150 160 170 180 LAVPMPEVLY EEVLEVDERV VLHRGEAGTG TPVKGRTGDL LEVQQPVDLG ALRGKLEGLL 190 200 210 220 230 240 SRGIRSLAVV LMHSYTWAQH EQQVGVLARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY 250 260 270 280 290 300 LTPAIQRYVQ GFCRGFQGQL KDVQVLFMRS DGGLAPMDTF SGSSAVLSGP AGGVVGYSAT 310 320 330 340 350 360 TYQQEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN TVAAGGGSRL 370 380 390 400 410 420 FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR LLPASFPCIF GPGENQPLSP 430 440 450 460 470 480 EASRKALEAV ATEVNSFLTN GPCPASPLSL EEVAMGFVRV ANEAMCRPIR ALTQARGHDP 490 500 510 520 530 540 SAHVLACFGG AGGQHACAIA RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLLYA 550 560 570 580 590 600 PETFVQLDQR LSRLEEQCVD ALQAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA 610 620 630 640 650 660 RSPRAGDFGA AFVERYMREF GFVIPERPVV VDDVRVRGTG RSGLRLEDAP KAQTGPPRVD 670 680 690 700 710 720 KMTQCYFEGG YQETPVYLLA ELGYGHKLHG PCLIIDSNST ILVEPGCQAE VTKTGDICIS 730 740 750 760 770 780 VGAEVPGTVG PQLDPIQLSI FSHRFMSIAE QMGRILQRTA ISTNIKERLD FSCALFGPDG 790 800 810 820 830 840 GLVSNAPHIP VHLGAMQETV QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW 850 860 870 880 890 900 PGQTRPVFYV ASRGHHADIG GITPGSMPPH STMLQQEGAV FLSFKLVQGG VFQEEAVTEA 910 920 930 940 950 960 LRAPGKVPNC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ AYMGHIQANA 970 980 990 1000 1010 1020 ELAVRDMLRA FGTSRQARGL PLEVSSEDHM DDGSPIRLRV QISLSQGSAV FDFSGTGPEV 1030 1040 1050 1060 1070 1080 FGNLNAPRAV TLSALIYCLR CLVGRDIPLN QGCLAPVRVV IPRGSILDPS PEAAVVGGNV 1090 1100 1110 1120 1130 1140 LTSQRVVDVI LGAFGACAAS QGCMNNVTLG NAHMGYYETV AGGAGAGPSW HGRSGVHSHM 1150 1160 1170 1180 1190 1200 TNTRITDPEI LESRYPVILR RFELRRGSGG RGRFRGGDGV TRELLFREEA LLSVLTERRA 1210 1220 1230 1240 1250 1260 FRPYGLHGGE PGARGLNLLI RKNGRTVNLG GKTSVTVYPG DVFCLHTPGG GGYGDPEDPA 1270 1280 PPPGSPPQAL AFPEHGSVYE YRRAQEAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery." Watanabe T., Abe K., Ishikawa H., Iijima Y. Biol. Pharm. Bull. 27:288-294(2004) [PubMed: 14993790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-1288. Tissue: Testis.
[4]	"Human 5-oxo-L-prolinase partial sequence." Debella L.R., Wood S. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 901-1096.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB122018 mRNA. Translation: BAD13434.1. BC142672 mRNA. Translation: AAI42673.1. AL096750 mRNA. Translation: CAB46426.2. Frameshift. AF024673, AF024672 Genomic DNA. Translation: AAB81519.1. Frameshift.
IPI	IPI00418382.
PIR	T12537.
RefSeq	NP_060040.1. NM_017570.3.
UniGene	Hs.305882.
3D structure databases
ProteinModelPortal	O14841.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14841. 1 interaction.
STRING	O14841.
Proteomic databases
PRIDE	O14841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000426825; ENSP00000412071; ENSG00000178814.
GeneID	26873.
KEGG	hsa:26873.
Organism-specific databases
CTD	26873.
GeneCards	GC08M145106.
H-InvDB	HIX0022242.
HGNC	HGNC:8149. OPLAH.
MIM	614243. gene.
neXtProt	NX_O14841.
PharmGKB	PA31935.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09696.
GeneTree	ENSGT00390000013463.
HOGENOM	HBG285674.
HOVERGEN	HBG007870.
InParanoid	O14841.
OMA	PLTVTDC.
OrthoDB	EOG4N8R3X.
PhylomeDB	O14841.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O14841.
Bgee	O14841.
CleanEx	HS_OPLAH.
Genevestigator	O14841.
GermOnline	ENSG00000178814. Homo sapiens.
Family and domain databases
InterPro	IPR008040. Hydant_A_N. IPR002821. Hydantoinase_A. IPR003692. Hydantoinase_B. [Graphical view]
KO	K01469.
Pfam	PF05378. Hydant_A_N. 1 hit. PF01968. Hydantoinase_A. 1 hit. PF02538. Hydantoinase_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00142. L-Glutamic Acid.
NextBio	49396.
SOURCE	Search...

Entry information

Entry name

OPLA_HUMAN

Accession

Primary (citable) accession number: O14841
Secondary accession number(s): A5PKY8, Q75W65, Q9Y4Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.