O14817 (TSN4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraspanin-4
Short name=Tspan-4

Alternative name(s):
Novel antigen 2
Short name=NAG-2
Transmembrane 4 superfamily member 7

Gene names

Name:	TSPAN4
Synonyms:	NAG2, TM4SF7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Forms a complex with integrins.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in multiple tissues but is absent in brain, lymphoid cells, and platelets.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein complex assembly Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Tetraspanin-4		PRO_0000219241
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Topological domain	35 – 55	21	Extracellular Potential
	Transmembrane	56 – 76	21	Helical; Potential
	Topological domain	77 – 85	9	Cytoplasmic Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Topological domain	107 – 201	95	Extracellular Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Topological domain	223 – 238	16	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Ref.8
Natural variations
	Natural variant	67	1	I → M in a breast cancer sample; somatic mutation. Ref.9		VAR_036616

Sequences

	Sequence		Length	Mass (Da)
	O14817 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A6B9A5633065A492	FASTA	238	26,118
	10 20 30 40 50 60 MARACLQAVK YLMFAFNLLF WLGGCGVLGV GIWLAATQGS FATLSSSFPS LSAANLLIIT 70 80 90 100 110 120 GAFVMAIGFV GCLGAIKENK CLLLTFFLLL LLVFLLEATI AILFFAYTDK IDRYAQQDLK 130 140 150 160 170 180 KGLHLYGTQG NVGLTNAWSI IQTDFRCCGV SNYTDWFEVY NATRVPDSCC LEFSESCGLH 190 200 210 220 230 APGTWWKAPC YETVKVWLQE NLLAVGIFGL CTALVQILGL TFAMTMYCQV VKADTYCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that complexes with integrins and other TM4SF proteins." Tachibana I., Bodorova J., Berditchevski F., Zutter M.M., Hemler M.E. J. Biol. Chem. 272:29181-29189(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequences and expression of six new members of the tetraspanin/TM4SF family." Todd S.C., Doctor V.S., Levy S. Biochim. Biophys. Acta 1399:101-104(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-161, MASS SPECTROMETRY. Tissue: Liver.
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-67.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022813 mRNA. Translation: AAC51864.1. AF054841 mRNA. Translation: AAC69717.1. AK291089 mRNA. Translation: BAF83778.1. BT006776 mRNA. Translation: AAP35422.1. CR457108 mRNA. Translation: CAG33389.1. CH471158 Genomic DNA. Translation: EAX02409.1. BC000389 mRNA. Translation: AAH00389.1. BC019314 mRNA. Translation: AAH19314.1. BC093031 mRNA. Translation: AAH93031.1.
IPI	IPI00024174.
PIR	A59265.
RefSeq	NP_001020405.1. NM_001025234.1. NP_001020406.1. NM_001025235.1. NP_001020407.1. NM_001025236.1. NP_001020408.1. NM_001025237.1. NP_001020409.1. NM_001025238.1. NP_001020410.1. NM_001025239.1. NP_003262.1. NM_003271.4.
UniGene	Hs.654836.
3D structure databases
ProteinModelPortal	O14817.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000324304.
Proteomic databases
PaxDb	O14817.
PRIDE	O14817.
Protocols and materials databases
DNASU	7106.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000397397; ENSP00000380552; ENSG00000214063. ENST00000397404; ENSP00000380553; ENSG00000214063. ENST00000397406; ENSP00000380554; ENSG00000214063. ENST00000397408; ENSP00000380555; ENSG00000214063. ENST00000397411; ENSP00000380558; ENSG00000214063. ENST00000409543; ENSP00000386513; ENSG00000214063.
GeneID	7106.
KEGG	hsa:7106.
UCSC	uc001lsd.1. human.
Organism-specific databases
CTD	7106.
GeneCards	GC11P000833.
HGNC	HGNC:11859. TSPAN4.
HPA	HPA016435.
MIM	602644. gene.
neXtProt	NX_O14817.
PharmGKB	PA36560.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG290117.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	O14817.
OrthoDB	EOG4KD6MV.
PhylomeDB	O14817.
Gene expression databases
ArrayExpress	O14817.
Bgee	O14817.
CleanEx	HS_TSPAN4.
Genevestigator	O14817.
GermOnline	ENSG00000177769. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TSPAN4. human.
GenomeRNAi	7106.
NextBio	27801.
SOURCE	Search...

Entry information

Entry name

TSN4_HUMAN

Accession

Primary (citable) accession number: O14817
Secondary accession number(s): Q6IAP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.