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Protein

Tetraspanin-4

Gene

TSPAN4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • antigen binding Source: UniProtKB
  • integrin binding Source: UniProtKB

GO - Biological processi

  • protein complex assembly Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraspanin-4
Short name:
Tspan-4
Alternative name(s):
Novel antigen 2
Short name:
NAG-2
Transmembrane 4 superfamily member 7
Gene namesi
Name:TSPAN4
Synonyms:NAG2, TM4SF7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11859. TSPAN4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence AnalysisAdd
BLAST
Topological domaini35 – 5521ExtracellularSequence AnalysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence AnalysisAdd
BLAST
Topological domaini77 – 859CytoplasmicSequence Analysis
Transmembranei86 – 10621HelicalSequence AnalysisAdd
BLAST
Topological domaini107 – 20195ExtracellularSequence AnalysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence AnalysisAdd
BLAST
Topological domaini223 – 23816CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • focal adhesion Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36560.

Polymorphism and mutation databases

BioMutaiTSPAN4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Tetraspanin-4PRO_0000219241Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi161 – 1611N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO14817.
PaxDbiO14817.
PRIDEiO14817.

Expressioni

Tissue specificityi

Expressed in multiple tissues but is absent in brain, lymphoid cells, and platelets.

Gene expression databases

BgeeiO14817.
CleanExiHS_TSPAN4.
ExpressionAtlasiO14817. baseline and differential.
GenevisibleiO14817. HS.

Organism-specific databases

HPAiHPA016435.

Interactioni

Subunit structurei

Forms a complex with integrins.

Binary interactionsi

WithEntry#Exp.IntActNotes
NOTCH2NLQ7Z3S93EBI-8652667,EBI-945833

Protein-protein interaction databases

BioGridi112961. 7 interactions.
IntActiO14817. 2 interactions.
STRINGi9606.ENSP00000380552.

Structurei

3D structure databases

ProteinModelPortaliO14817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290117.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiO14817.
KOiK17294.
OrthoDBiEOG7PGDRZ.
PhylomeDBiO14817.
TreeFamiTF352892.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARACLQAVK YLMFAFNLLF WLGGCGVLGV GIWLAATQGS FATLSSSFPS
60 70 80 90 100
LSAANLLIIT GAFVMAIGFV GCLGAIKENK CLLLTFFLLL LLVFLLEATI
110 120 130 140 150
AILFFAYTDK IDRYAQQDLK KGLHLYGTQG NVGLTNAWSI IQTDFRCCGV
160 170 180 190 200
SNYTDWFEVY NATRVPDSCC LEFSESCGLH APGTWWKAPC YETVKVWLQE
210 220 230
NLLAVGIFGL CTALVQILGL TFAMTMYCQV VKADTYCA
Length:238
Mass (Da):26,118
Last modified:January 1, 1998 - v1
Checksum:iA6B9A5633065A492
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671I → M in a breast cancer sample; somatic mutation. 1 Publication
VAR_036616

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022813 mRNA. Translation: AAC51864.1.
AF054841 mRNA. Translation: AAC69717.1.
AK291089 mRNA. Translation: BAF83778.1.
BT006776 mRNA. Translation: AAP35422.1.
CR457108 mRNA. Translation: CAG33389.1.
CH471158 Genomic DNA. Translation: EAX02409.1.
BC000389 mRNA. Translation: AAH00389.1.
BC019314 mRNA. Translation: AAH19314.1.
BC093031 mRNA. Translation: AAH93031.1.
CCDSiCCDS7721.1.
PIRiA59265.
RefSeqiNP_001020405.1. NM_001025234.1.
NP_001020406.1. NM_001025235.1.
NP_001020407.1. NM_001025236.1.
NP_001020408.1. NM_001025237.1.
NP_001020409.1. NM_001025238.1.
NP_001020410.1. NM_001025239.1.
NP_003262.1. NM_003271.4.
XP_005253159.1. XM_005253102.1.
UniGeneiHs.654836.

Genome annotation databases

EnsembliENST00000397397; ENSP00000380552; ENSG00000214063.
ENST00000397404; ENSP00000380553; ENSG00000214063.
ENST00000397406; ENSP00000380554; ENSG00000214063.
ENST00000397408; ENSP00000380555; ENSG00000214063.
ENST00000397411; ENSP00000380558; ENSG00000214063.
ENST00000409543; ENSP00000386513; ENSG00000214063.
GeneIDi7106.
KEGGihsa:7106.
UCSCiuc001lsd.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022813 mRNA. Translation: AAC51864.1.
AF054841 mRNA. Translation: AAC69717.1.
AK291089 mRNA. Translation: BAF83778.1.
BT006776 mRNA. Translation: AAP35422.1.
CR457108 mRNA. Translation: CAG33389.1.
CH471158 Genomic DNA. Translation: EAX02409.1.
BC000389 mRNA. Translation: AAH00389.1.
BC019314 mRNA. Translation: AAH19314.1.
BC093031 mRNA. Translation: AAH93031.1.
CCDSiCCDS7721.1.
PIRiA59265.
RefSeqiNP_001020405.1. NM_001025234.1.
NP_001020406.1. NM_001025235.1.
NP_001020407.1. NM_001025236.1.
NP_001020408.1. NM_001025237.1.
NP_001020409.1. NM_001025238.1.
NP_001020410.1. NM_001025239.1.
NP_003262.1. NM_003271.4.
XP_005253159.1. XM_005253102.1.
UniGeneiHs.654836.

3D structure databases

ProteinModelPortaliO14817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112961. 7 interactions.
IntActiO14817. 2 interactions.
STRINGi9606.ENSP00000380552.

Polymorphism and mutation databases

BioMutaiTSPAN4.

Proteomic databases

MaxQBiO14817.
PaxDbiO14817.
PRIDEiO14817.

Protocols and materials databases

DNASUi7106.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397397; ENSP00000380552; ENSG00000214063.
ENST00000397404; ENSP00000380553; ENSG00000214063.
ENST00000397406; ENSP00000380554; ENSG00000214063.
ENST00000397408; ENSP00000380555; ENSG00000214063.
ENST00000397411; ENSP00000380558; ENSG00000214063.
ENST00000409543; ENSP00000386513; ENSG00000214063.
GeneIDi7106.
KEGGihsa:7106.
UCSCiuc001lsd.1. human.

Organism-specific databases

CTDi7106.
GeneCardsiGC11P000843.
HGNCiHGNC:11859. TSPAN4.
HPAiHPA016435.
MIMi602644. gene.
neXtProtiNX_O14817.
PharmGKBiPA36560.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG290117.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiO14817.
KOiK17294.
OrthoDBiEOG7PGDRZ.
PhylomeDBiO14817.
TreeFamiTF352892.

Miscellaneous databases

ChiTaRSiTSPAN4. human.
GeneWikiiTSPAN4.
GenomeRNAii7106.
NextBioi27801.
PROiO14817.
SOURCEiSearch...

Gene expression databases

BgeeiO14817.
CleanExiHS_TSPAN4.
ExpressionAtlasiO14817. baseline and differential.
GenevisibleiO14817. HS.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that complexes with integrins and other TM4SF proteins."
    Tachibana I., Bodorova J., Berditchevski F., Zutter M.M., Hemler M.E.
    J. Biol. Chem. 272:29181-29189(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequences and expression of six new members of the tetraspanin/TM4SF family."
    Todd S.C., Doctor V.S., Levy S.
    Biochim. Biophys. Acta 1399:101-104(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Teratocarcinoma.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  8. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-161.
    Tissue: Liver.
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-67.

Entry informationi

Entry nameiTSN4_HUMAN
AccessioniPrimary (citable) accession number: O14817
Secondary accession number(s): Q6IAP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.