ID   CPLX1_HUMAN             Reviewed;         134 AA.
AC   O14810; B2R4R5;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-NOV-2009, entry version 67.
DE   RecName: Full=Complexin-1;
DE   AltName: Full=Complexin I;
DE            Short=CPX I;
DE   AltName: Full=Synaphin-2;
GN   Name=CPLX1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   MEDLINE=96006530; PubMed=7553862; DOI=10.1016/0092-8674(95)90239-2;
RA   McMahon H.T., Missler M., Li C., Suedhof T.C.;
RT   "Complexins: cytosolic proteins that regulate SNAP receptor
RT   function.";
RL   Cell 83:111-119(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Cerebellum;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA   Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA   Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA   Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA   Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA   Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA   Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA   Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA   Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA   Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA   Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA   Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA   Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA   Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA   Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA   Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA   Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA   Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA   McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA   Waterston R.H., Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2
RT   and 4.";
RL   Nature 434:724-731(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=9853440; DOI=10.1016/S0140-6736(98)03341-8;
RA   Harrison P.J., Eastwood S.L.;
RT   "Preferential involvement of excitatory neurons in medial temporal
RT   lobe in schizophrenia.";
RL   Lancet 352:1669-1673(1998).
RN   [8]
RP   TISSUE SPECIFICITY.
RX   PubMed=11483314; DOI=10.1016/S0306-4522(01)00141-5;
RA   Eastwood S.L., Cotter D., Harrison P.J.;
RT   "Cerebellar synaptic protein expression in schizophrenia.";
RL   Neuroscience 105:219-229(2001).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RX   PubMed=15526345; DOI=10.1002/pmic.200400848;
RA   Basso M., Giraudo S., Corpillo D., Bergamasco B., Lopiano L.,
RA   Fasano M.;
RT   "Proteome analysis of human substantia nigra in Parkinson's disease.";
RL   Proteomics 4:3943-3952(2004).
CC   -!- FUNCTION: Positively regulates a late step in synaptic vesicle
CC       exocytosis. Also involved in glucose-induced secretion of insulin
CC       by pancreatic beta-cells (By similarity).
CC   -!- SUBUNIT: Binds to the SNARE core complex containing SNAP25, VAMP2
CC       and STX1A (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity).
CC       Note=Enriched at synaptic-releasing sites in mature neurons (By
CC       similarity).
CC   -!- TISSUE SPECIFICITY: Nervous system. In hippocampus and cerebellum,
CC       expressed mainly by inhibitory neurons. Overexpressed in
CC       substantia nigra from patients with Parkinson disease.
CC   -!- SIMILARITY: Belongs to the complexin/synaphin family.
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DR   EMBL; AF022383; AAB72108.1; -; mRNA.
DR   EMBL; BT007029; AAP35676.1; -; mRNA.
DR   EMBL; AK311921; BAG34862.1; -; mRNA.
DR   EMBL; AC139887; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471131; EAW82648.1; -; Genomic_DNA.
DR   EMBL; BC002471; AAH02471.1; -; mRNA.
DR   IPI; IPI00396023; -.
DR   RefSeq; NP_006642.1; -.
DR   UniGene; Hs.478930; -.
DR   HSSP; Q64276; 1KIL.
DR   PhosphoSite; O14810; -.
DR   PeptideAtlas; O14810; -.
DR   PRIDE; O14810; -.
DR   Ensembl; ENST00000304062; ENSP00000305613; ENSG00000168993; Homo sapiens.
DR   Ensembl; ENST00000445104; ENSP00000414681; ENSG00000168993; Homo sapiens.
DR   GeneID; 10815; -.
DR   KEGG; hsa:10815; -.
DR   UCSC; uc003gbi.1; human.
DR   CTD; 10815; -.
DR   GeneCards; GC04M000771; -.
DR   H-InvDB; HIX0004006; -.
DR   HGNC; HGNC:2309; CPLX1.
DR   MIM; 605032; gene.
DR   PharmGKB; PA26826; -.
DR   HOVERGEN; O14810; -.
DR   Reactome; REACT_13685; Synaptic Transmission.
DR   NextBio; 41085; -.
DR   Bgee; O14810; -.
DR   CleanEx; HS_CPLX1; -.
DR   Genevestigator; O14810; -.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0019905; F:syntaxin binding; IEA:InterPro.
DR   GO; GO:0006887; P:exocytosis; TAS:ProtInc.
DR   GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR   GO; GO:0007268; P:synaptic transmission; TAS:ProtInc.
DR   InterPro; IPR008849; Synaphin.
DR   PANTHER; PTHR16705; Synaphin; 1.
DR   Pfam; PF05835; Synaphin; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Complete proteome; Cytoplasm; Exocytosis;
KW   Neurotransmitter transport; Transport.
FT   CHAIN         1    134       Complexin-1.
FT                                /FTId=PRO_0000144870.
FT   REGION       48     70       Interaction with the SNARE complex (By
FT                                similarity).
FT   COILED       29     69       Potential.
SQ   SEQUENCE   134 AA;  15030 MW;  A7A52F17F10D28A4 CRC64;
     MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE
     AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV
     IKYLPGPLQD MLKK
//