ID   CPLX1_HUMAN             Reviewed;         134 AA.
AC   O14810; A6NI80; B2R4R5; D3DVN3;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-JAN-2012, entry version 85.
DE   RecName: Full=Complexin-1;
DE   AltName: Full=Complexin I;
DE            Short=CPX I;
DE   AltName: Full=Synaphin-2;
GN   Name=CPLX1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   MEDLINE=96006530; PubMed=7553862; DOI=10.1016/0092-8674(95)90239-2;
RA   McMahon H.T., Missler M., Li C., Suedhof T.C.;
RT   "Complexins: cytosolic proteins that regulate SNAP receptor
RT   function.";
RL   Cell 83:111-119(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Cerebellum;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA   Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA   Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA   Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA   Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA   Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA   Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA   Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA   Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA   Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA   Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA   Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA   Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA   Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA   Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA   Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA   Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA   Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA   McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA   Waterston R.H., Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2
RT   and 4.";
RL   Nature 434:724-731(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=9853440; DOI=10.1016/S0140-6736(98)03341-8;
RA   Harrison P.J., Eastwood S.L.;
RT   "Preferential involvement of excitatory neurons in medial temporal
RT   lobe in schizophrenia.";
RL   Lancet 352:1669-1673(1998).
RN   [8]
RP   TISSUE SPECIFICITY.
RX   PubMed=11483314; DOI=10.1016/S0306-4522(01)00141-5;
RA   Eastwood S.L., Cotter D., Harrison P.J.;
RT   "Cerebellar synaptic protein expression in schizophrenia.";
RL   Neuroscience 105:219-229(2001).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RX   PubMed=15526345; DOI=10.1002/pmic.200400848;
RA   Basso M., Giraudo S., Corpillo D., Bergamasco B., Lopiano L.,
RA   Fasano M.;
RT   "Proteome analysis of human substantia nigra in Parkinson's disease.";
RL   Proteomics 4:3943-3952(2004).
RN   [10]
RP   X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 28-83 ALONE AND IN COMPLEX
RP   WITH SNAP25; VAMP2 AND RAT STX1A, SUBUNIT, AND FUNCTION.
RX   PubMed=21785414; DOI=10.1038/nsmb.2101;
RA   Kummel D., Krishnakumar S.S., Radoff D.T., Li F., Giraudo C.G.,
RA   Pincet F., Rothman J.E., Reinisch K.M.;
RT   "Complexin cross-links prefusion SNAREs into a zigzag array.";
RL   Nat. Struct. Mol. Biol. 18:927-933(2011).
CC   -!- FUNCTION: Positively regulates a late step in synaptic vesicle
CC       exocytosis. Organizes the SNAREs into a cross-linked zigzag
CC       topology that, when interposed between the vesicle and plasma
CC       membranes, is incompatible with fusion, thereby preventing SNAREs
CC       from releasing neurotransmitters until an action potential arrives
CC       at the synapse. Also involved in glucose-induced secretion of
CC       insulin by pancreatic beta-cells (By similarity).
CC   -!- SUBUNIT: Binds to the SNARE core complex containing SNAP25, VAMP2
CC       and STX1A.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity).
CC       Note=Enriched at synaptic-releasing sites in mature neurons (By
CC       similarity).
CC   -!- TISSUE SPECIFICITY: Nervous system. In hippocampus and cerebellum,
CC       expressed mainly by inhibitory neurons. Overexpressed in
CC       substantia nigra from patients with Parkinson disease.
CC   -!- SIMILARITY: Belongs to the complexin/synaphin family.
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DR   EMBL; AF022383; AAB72108.1; -; mRNA.
DR   EMBL; BT007029; AAP35676.1; -; mRNA.
DR   EMBL; AK311921; BAG34862.1; -; mRNA.
DR   EMBL; AC139887; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471131; EAW82648.1; -; Genomic_DNA.
DR   EMBL; CH471131; EAW82649.1; -; Genomic_DNA.
DR   EMBL; BC002471; AAH02471.1; -; mRNA.
DR   IPI; IPI00396023; -.
DR   RefSeq; NP_006642.1; NM_006651.3.
DR   UniGene; Hs.478930; -.
DR   PDB; 3RK3; X-ray; 3.50 A; E=28-83.
DR   PDB; 3RL0; X-ray; 3.80 A; g/h/i/j/k/l/m/n=28-83.
DR   PDBsum; 3RK3; -.
DR   PDBsum; 3RL0; -.
DR   ProteinModelPortal; O14810; -.
DR   SMR; O14810; 32-72.
DR   DIP; DIP-56109N; -.
DR   IntAct; O14810; 2.
DR   STRING; O14810; -.
DR   PhosphoSite; O14810; -.
DR   PeptideAtlas; O14810; -.
DR   PRIDE; O14810; -.
DR   Ensembl; ENST00000304062; ENSP00000305613; ENSG00000168993.
DR   GeneID; 10815; -.
DR   KEGG; hsa:10815; -.
DR   UCSC; uc003gbi.1; human.
DR   CTD; 10815; -.
DR   GeneCards; GC04M000778; -.
DR   H-InvDB; HIX0004006; -.
DR   HGNC; HGNC:2309; CPLX1.
DR   MIM; 605032; gene.
DR   neXtProt; NX_O14810; -.
DR   PharmGKB; PA26826; -.
DR   GeneTree; ENSGT00430000033109; -.
DR   HOVERGEN; HBG062792; -.
DR   OMA; SIVDTVM; -.
DR   OrthoDB; EOG44MXTP; -.
DR   Reactome; REACT_13685; Neuronal System.
DR   NextBio; 41085; -.
DR   ArrayExpress; O14810; -.
DR   Bgee; O14810; -.
DR   CleanEx; HS_CPLX1; -.
DR   Genevestigator; O14810; -.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0014047; P:glutamate secretion; TAS:Reactome.
DR   InterPro; IPR008849; Synaphin.
DR   KO; K15294; -.
DR   PANTHER; PTHR16705; Synaphin; 1.
DR   Pfam; PF05835; Synaphin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Coiled coil; Complete proteome; Cytoplasm; Exocytosis;
KW   Neurotransmitter transport; Reference proteome; Transport.
FT   CHAIN         1    134       Complexin-1.
FT                                /FTId=PRO_0000144870.
FT   REGION       48     70       Interaction with the SNARE complex (By
FT                                similarity).
FT   COILED       29     69       Potential.
FT   HELIX        29     70
SQ   SEQUENCE   134 AA;  15030 MW;  A7A52F17F10D28A4 CRC64;
     MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE
     AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV
     IKYLPGPLQD MLKK
//